LOCUS       sequence-267692-       10816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant nSpyCas9 at amino acid position 
            1029 encoding truncated human cytosine deaminase, hAIDx, at the 
            C-terminus. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: 1029-AIDx-CP
FEATURES             Location/Qualifiers
     source          1..10816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        128..507
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        508..711
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     662..682
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     756..775
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        756..774
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4992..5012
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5028..5048
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5670..5717
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5718..5741
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5751..5807
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6300..6317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6306..6530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6576..7004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6663..6682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6873..6894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7013..7033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7018..7347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7198..7333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7260..7279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7414..8208
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7468..7487)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8078..8097
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8382..8503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8419..8438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8473..8492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8552..8568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8552..8568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8565..8587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8576..8592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8600..8606,8607..8624,8625..8630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8645..8666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8783..8800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8954..9542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9034..9053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9713..10504,10505..10573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10336..10355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10574..10678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10753..10772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
       61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      721 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      781 caagctggct agcgtttaaa cttaagcttg gtaccgccac catgataggc aaggcaaccg
      841 ctaagtattt tttttacagc aacatcatga acttctttaa gaccgagatt accctggcca
      901 acggtgaaat ccgtaaacgc ccgctgattg aaaccaacgg tgaaaccggc gaaatcgtgt
      961 gggacaaggg ccgtgatttt gcgaccgtgc gcaaagtgct gtccatgccg caggtgaaca
     1021 ttgtgaagaa aaccgaggtg cagaccggcg gtttcagcaa ggaaagcatt ctgccgaaac
     1081 gtaacagcga caagctgatc gcccgcaaga aagattggga cccgaagaaa tacggcggtt
     1141 tcgatagccc gaccgtggct tatagcgtgc tggtggtggc caaagtggag aagggtaaaa
     1201 gcaagaaact gaagagcgtg aaagaactgc tgggcatcac cattatggag cgtagcagct
     1261 tcgaaaagaa cccgatcgac tttctggagg cgaagggtta caaggaagtg aagaaagatc
     1321 tgatcattaa gctgccgaaa tacagcctgt ttgagctgga aaatggtcgt aaacgtatgc
     1381 tggccagcgc cggcgagctg caaaagggca acgaactggc gctgccgagc aaatatgtga
     1441 acttcctgta cctggccagc cactatgaga agctgaaagg tagcccggag gacaacgaac
     1501 agaagcagct gtttgtggag cagcacaaac actatctgga tgagatcatt gaacagatca
     1561 gcgagttcag caagcgtgtg attctggcgg acgctaacct ggataaagtg ctgagcgcct
     1621 acaacaaaca ccgtgacaag ccgatccgcg agcaggcgga aaacatcatt cacctgttca
     1681 ccctgaccaa cctgggtgcg ccggccgcgt tcaagtactt tgacaccacc attgatcgta
     1741 aacgctatac cagcaccaag gaggtgctgg atgctaccct gatccaccag agcattaccg
     1801 gcctgtacga aacccgtatt gatctgagcc agctgggcgg tgacggcggt agcggcggta
     1861 gcggcggtag cggcggtagc ggcggtagcg gcggtagcgg cggtgacaag aaatatagca
     1921 tcggtctggc gattggcacc aacagcgtgg gttgggctgt gatcaccgat gagtacaagg
     1981 tgccgagcaa gaagttcaag gtgctgggca acaccgaccg ccacagcatc aagaaaaacc
     2041 tgattggtgc cctgctgttt gatagcggtg aaaccgccga ggccacccgt ctgaagcgca
     2101 ccgcccgtcg ccgttacacc cgtcgtaaaa accgtatctg ctacctgcaa gagattttca
     2161 gcaacgaaat ggccaaagtg gacgatagct tctttcaccg cctggaggag agcttcctgg
     2221 tggaggaaga caagaaacac gagcgtcacc cgatctttgg caacattgtg gatgaggtgg
     2281 cttaccacga aaagtatccg accatctacc acctgcgtaa gaaactggtg gatagcaccg
     2341 acaaggccga tctgcgcctg atctatctgg ctctggccca catgatcaag ttccgtggtc
     2401 actttctgat cgagggcgac ctgaacccgg ataacagcga tgtggacaag ctgttcatcc
     2461 agctggtgca gacctacaac cagctgtttg aggaaaaccc gattaacgcc agcggtgtgg
     2521 acgcgaaggc tatcctgagc gcgcgtctga gcaaaagccg ccgtctggag aacctgatcg
     2581 cgcagctgcc gggtgaaaag aaaaacggtc tgtttggcaa cctgattgct ctgagcctgg
     2641 gcctgacccc gaacttcaaa agcaactttg acctggcgga ggatgctaag ctgcaactga
     2701 gcaaggatac ctatgacgat gacctggaca acctgctggc gcagatcggc gaccagtacg
     2761 ctgatctgtt cctggctgcc aagaacctga gcgatgcgat cctgctgagc gacattctgc
     2821 gtgtgaacac cgagattacc aaggccccgc tgagcgcgag catgatcaaa cgctatgatg
     2881 aacaccacca ggacctgacc ctgctgaaag ctctggtgcg tcagcagctg ccggagaagt
     2941 acaaagaaat cttctttgac cagagcaaga acggttacgc cggctatatt gatggcggtg
     3001 cgagccagga ggagttctac aagtttatca aaccgattct ggagaaaatg gacggcaccg
     3061 aggaactgct ggtgaagctg aaccgtgaag atctgctgcg taaacagcgc accttcgaca
     3121 acggtagcat cccgcaccag attcacctgg gtgagctgca cgccatcctg cgccgtcagg
     3181 aagatttcta cccgtttctg aaggacaacc gtgagaagat cgaaaaaatt ctgaccttcc
     3241 gcattccgta ctatgtgggt ccgctggctc gtggcaacag ccgctttgcc tggatgaccc
     3301 gcaagagcga ggaaaccatc accccgtgga acttcgagga agtggtggac aaaggcgcca
     3361 gcgcgcagag cttcattgag cgtatgacca actttgataa gaacctgccg aacgaaaaag
     3421 tgctgccgaa gcacagcctg ctgtacgagt atttcaccgt gtacaacgaa ctgaccaagg
     3481 tgaaatatgt gaccgagggt atgcgcaagc cggccttcct gagcggcgaa cagaagaaag
     3541 cgatcgtgga cctgctgttt aaaaccaacc gtaaggtgac cgtgaagcag ctgaaagagg
     3601 attatttcaa gaaaattgaa tgctttgaca gcgtggagat cagcggtgtg gaagatcgtt
     3661 ttaacgcgag cctgggcacc taccacgacc tgctgaaaat cattaaggat aaagacttcc
     3721 tggataacga ggaaaacgag gacatcctgg aagatattgt gctgaccctg accctgtttg
     3781 aggaccgtga aatgatcgag gaacgcctga agacctatgc ccacctgttc gatgacaaag
     3841 tgatgaaaca gctgaagcgc cgtcgctaca ccggttgggg ccgtctgagc cgcaaactga
     3901 tcaacggtat tcgcgataaa cagagcggca agaccatcct ggattttctg aagagcgacg
     3961 gtttcgcgaa ccgtaacttt atgcagctga ttcacgatga cagcctgacc ttcaaagagg
     4021 acatccagaa ggcgcaggtg agcggtcagg gcgatagcct gcacgaacat attgctaacc
     4081 tggccggtag cccggccatc aagaagggca tcctgcaaac cgtgaaggtg gtggacgagc
     4141 tggtgaaagt gatgggtcgt cacaagccgg aaaacatcgt gattgagatg gcccgcgaaa
     4201 accagaccac ccagaaaggc cagaagaaca gccgtgagcg catgaagcgt atcgaggaag
     4261 gcatcaagga actgggcagc cagatcctga aggagcaccc ggtggaaaac acccagctgc
     4321 aaaacgagaa actgtacctg tactacctgc aaaacggtcg tgacatgtat gtggatcagg
     4381 aactggacat taaccgcctg agcgattatg acgtggatgc gatcgtgccg cagagcttcc
     4441 tgaaggatga cagcattgac aacaaagtgc tgacccgtag cgataaaaac cgcggcaaga
     4501 gcgacaacgt gccgagcgag gaagtggtga agaaaatgaa aaactactgg cgtcagctgc
     4561 tgaacgccaa actgatcacc cagcgtaagt ttgacaacct gaccaaagct gagcgcggcg
     4621 gtctgagcga actggataaa gccggtttca ttaagcgtca gctggtggaa acccgccaga
     4681 tcaccaagca cgtggcgcag attctggaca gccgcatgaa caccaagtat gacgagaacg
     4741 ataaactgat ccgtgaagtg aaagtgatca ccctgaagag caaactggtg agcgattttc
     4801 gtaaagactt ccagttttac aaggtgcgcg agatcaacaa ctaccaccac gctcacgacg
     4861 cctatctgaa cgcggtggtg ggcaccgccc tgatcaagaa gtacccgaag ctggagagcg
     4921 agttcgtgta cggcgactac aaggtgtatg atgtgcgtaa gatgatcgcc aaaagcgagc
     4981 aggaaattgg cccgaagaaa aagcgtaaag tgggtagcag cggcagcccg aaaaagaaac
     5041 gcaaagtggg tagcgacgct ctggatgact tcgacctgga tatgctgggc agcgacgccc
     5101 tggatgactt tggcggtggc agcatggata gcctgctgat gaaccgtcgc aaattcctgt
     5161 atcagtttaa gaacgtgcgt tgggctaaag gccgtcgcga aacctacctg tgctatgtgg
     5221 tgaaacgtcg cgatagcgcc accagcttca gcctggactt tggttacctg cgcaacaaga
     5281 acggctgcca cgtggagctg ctgttcctgc gttacattag cgactgggac ctggacccag
     5341 gccgttgcta tcgcgtgacc tggtttacca gctggagccc gtgctacgat tgcgctcgtc
     5401 atgtggcgga cttcctgcgt ggtaacccaa acctgagcct gcgtatcttc accgcgcgcc
     5461 tgtacttttg cgaggaccgt aaggctgagc cggaaggtct gcgtcgcctg caccgtgcgg
     5521 gtgtgcagat cgcgatcatg accttcaaag attactttta ttgctggaac accttcgtgg
     5581 agaaccacga acgcaccttt aaggcctggg agggtctgca cgaaaacagc gtgcgtctga
     5641 gccgccagct gcgccgcatc ctgctgccaa agcgtccggc ggctaccaag aaagcgggcc
     5701 aggctaagaa aaagaaagac tacaaagatg acgatgacaa gggtagcggc gctaccaact
     5761 tcagcctgct gaagcaggcg ggcgacgtgg aggaaaatcc gggcccgatg gctaaaccgc
     5821 tgagccagga ggaaagcacc ctgatcgagc gcgctaccgc caccattaac agcatcccga
     5881 ttagcgaaga ctatagcgtg gccagcgccg cgctgagcag cgatggtcgt attttcaccg
     5941 gcgtgaacgt gtaccacttt accggtggcc cgtgcgccga gctggtggtg ctgggcaccg
     6001 ccgccgccgc cgccgccggc aacctgacct gcatcgtggc gattggtaac gaaaatcgtg
     6061 gcatcctgag cccgtgcggc cgttgccgcc aggtgctgct ggacctgcat ccgggcatca
     6121 aggcgattgt gaaggatagc gacggccaac cgaccgccgt gggtattcgt gaactgctgc
     6181 catccggcta tgtttgggag ggctaaggat ccactagtcc agtgtggtgg aattctgcag
     6241 atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc
     6301 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     6361 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     6421 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     6481 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     6541 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     6601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     7021 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7081 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     7141 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     7201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7261 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     7321 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     7381 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     7441 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7501 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7561 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7621 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7681 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7741 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7801 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7861 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7921 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     7981 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8041 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8101 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8161 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8221 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     8281 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8341 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8521 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8581 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8641 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8701 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8761 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8821 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8881 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8941 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9001 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9061 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9121 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9181 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9241 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9301 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9361 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9421 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9481 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9601 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9661 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9721 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9781 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9841 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9901 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9961 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10021 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10081 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10141 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10201 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10261 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10321 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10381 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10441 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10501 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10561 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10621 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10681 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10741 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    10801 ttgtgtgttg gaggtc
//
