LOCUS       sequence-267693-       10756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of wildtype nSpyCas9 encoding truncated human cytosine 
            deaminase, hAIDx, at the C-terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: SpyCas9-AIDx
FEATURES             Location/Qualifiers
     source          1..10756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        171..550
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        551..754
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     705..725
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     799..818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        799..817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      853..862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(859..885,886..888,889..3375,3376..3378,3379..4962)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4963..4983
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4999..5019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5641..5688
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5689..5712
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5722..5778
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6283..6300)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6289..6513
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6559..6987
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6646..6665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6856..6877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6996..7016)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7001..7330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7181..7316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7243..7262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7397..8191
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7451..7470)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8061..8080
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8365..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8402..8421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8456..8475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8535..8551)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8535..8551)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8548..8570)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8559..8575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8583..8589,8590..8607,8608..8613))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8628..8649
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8766..8783)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8937..9525)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9017..9036)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9696..10487,10488..10556))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10319..10338
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10557..10661)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10736..10755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
       61 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      121 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      181 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      721 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      781 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt
      841 aaacttaagc ttgccaccat ggataaaaag tatagcattg gtctggcgat tggcaccaac
      901 agcgtgggct gggccgtgat taccgatgag tacaaagttc cgtccaagaa gttcaaggtg
      961 ctgggtaaca ccgaccgcca cagcatcaag aaaaacctga ttggtgccct gctgtttgat
     1021 agcggtgaaa ccgccgaggc tacccgtctg aagcgcaccg cccgtcgccg ttatacccgt
     1081 cgtaaaaacc gtatctgcta cctgcaagag attttcagca acgaaatggc caaagtggac
     1141 gatagcttct ttcaccgcct ggaggagagc ttcctggtgg aggaagacaa gaaacacgag
     1201 cgtcacccga tctttggcaa cattgtggat gaggtggctt atcacgaaaa gtacccgacc
     1261 atctatcacc tgcgtaagaa actggtggat agcaccgaca aggccgatct gcgcctgatc
     1321 tacctggcgc tggctcacat gatcaagttc cgtggtcact ttctgatcga gggcgacctg
     1381 aacccggata acagcgatgt ggacaagctg ttcatccagc tggtgcagac ctataaccag
     1441 ctgtttgagg aaaacccgat taacgccagc ggtgtggacg ccaaggcgat cctgagcgcg
     1501 cgtctgagca aaagccgccg tctggagaac ctgatcgcgc agctgccggg tgaaaagaaa
     1561 aacggtctgt ttggcaacct gattgctctg agcctgggcc tgaccccgaa cttcaaaagc
     1621 aactttgacc tggccgagga tgcgaagctg caactgagca aggataccta cgacgatgac
     1681 ctggacaacc tgctggcgca gatcggcgac cagtatgctg atctgttcct ggccgcgaag
     1741 aacctgagcg atgcgatcct gctgagcgac attctgcgtg tgaacaccga gattaccaag
     1801 gctccgctga gcgccagcat gatcaaacgc tacgatgaac accaccagga cctgaccctg
     1861 ctgaaagctc tggtgcgtca gcagctgccg gagaagtata aagaaatctt ctttgaccag
     1921 agcaagaacg gttacgccgg ctatattgat ggcggtgcga gccaggagga gttctacaag
     1981 tttatcaaac cgattctgga gaaaatggac ggcaccgagg aactgctggt gaagctgaac
     2041 cgtgaagatc tgctgcgtaa acagcgcacc tttgacaacg gtagcatccc gcaccagatt
     2101 cacctgggtg agctgcacgc catcctgcgc cgtcaggaag atttctaccc gtttctgaag
     2161 gacaaccgtg agaagatcga aaaaattctg accttccgca ttccgtacta tgtgggtccg
     2221 ctggctcgtg gcaacagccg ctttgcctgg atgacccgca agagcgagga aaccatcacc
     2281 ccgtggaact tcgaggaagt ggtggacaaa ggcgccagcg cccagagctt cattgagcgt
     2341 atgaccaact ttgataagaa cctgccgaac gaaaaagtgc tgccgaagca cagcctgctg
     2401 tacgagtatt tcaccgtgta taacgaactg accaaggtga aatacgtgac cgagggtatg
     2461 cgcaagccgg ccttcctgag cggcgaacag aagaaagcga tcgtggacct gctgtttaaa
     2521 accaaccgta aggtgaccgt gaagcagctg aaagaggatt acttcaagaa aattgaatgc
     2581 ttcgacagcg tggagatcag cggtgtggaa gatcgtttta acgcgagcct gggcacctat
     2641 cacgacctgc tgaaaatcat taaggataaa gacttcctgg ataacgagga aaacgaggac
     2701 atcctggaag atattgtgct gaccctgacc ctgtttgagg accgtgaaat gatcgaggaa
     2761 cgcctgaaga cctacgccca cctgttcgat gacaaagtga tgaaacagct gaagcgccgt
     2821 cgctataccg gttggggccg tctgagccgc aaactgatca acggtattcg cgataaacag
     2881 agcggcaaga ccatcctgga ttttctgaag agcgacggtt tcgcgaaccg taactttatg
     2941 cagctgattc acgatgacag cctgaccttc aaagaggaca tccagaaggc gcaggtgagc
     3001 ggtcagggcg atagcctgca cgaacacatc gctaacctgg ccggtagccc ggccatcaag
     3061 aagggcatcc tgcaaaccgt gaaggtggtg gacgagctgg tgaaagtgat gggtcgtcac
     3121 aagccggaaa acatcgtgat tgagatggcc cgcgaaaacc agaccaccca gaaaggccag
     3181 aagaacagcc gtgagcgcat gaagcgtatc gaggaaggca tcaaggaact gggcagccag
     3241 atcctgaagg agcacccggt ggaaaacacc cagctgcaaa acgagaaact gtatctgtac
     3301 tacctgcaaa acggtcgtga catgtacgtg gatcaggagc tggacattaa ccgcctgagc
     3361 gattacgacg tggatgcgat cgtgccgcag agcttcctga aggatgacag cattgacaac
     3421 aaagtgctga cccgtagcga taaaaaccgc ggcaagagcg acaacgtgcc gagcgaggaa
     3481 gtggtgaaga aaatgaaaaa ctactggcgt cagctgctga acgccaaact gatcacccag
     3541 cgtaagtttg acaacctgac caaagctgag cgcggcggtc tgagcgaact ggataaagcc
     3601 ggtttcatta agcgtcagct ggtggaaacc cgccagatca ccaagcacgt ggcgcagatt
     3661 ctggacagcc gcatgaacac caagtacgac gagaacgata aactgatccg tgaagtgaaa
     3721 gtgatcaccc tgaagagcaa actggtgagc gattttcgta aagacttcca gttttacaag
     3781 gtgcgcgaga tcaacaacta tcaccacgcg cacgacgctt acctgaacgc cgtggtgggc
     3841 accgccctga tcaagaagta tccgaagctg gagagcgagt tcgtgtacgg cgattataaa
     3901 gtgtacgacg tgcgtaaaat gatcgcgaag agcgagcagg aaattggcaa agcgaccgct
     3961 aagtatttct tttacagcaa catcatgaac ttctttaaga ccgagatcac cctggccaac
     4021 ggtgaaatcc gtaaacgccc gctgattgaa accaacggtg aaaccggcga aatcgtgtgg
     4081 gataagggcc gtgactttgc gaccgtgcgc aaagtgctgt ccatgccgca ggtgaacatt
     4141 gtgaagaaaa ccgaggtgca gaccggcggt ttcagcaagg aaagcattct gccgaaacgt
     4201 aacagcgaca agctgatcgc ccgcaagaaa gactgggacc cgaagaagta tggcggtttc
     4261 gatagcccaa ccgtggccta cagcgtgctg gtggtggcta aagtggagaa gggtaaaagc
     4321 aagaaactga aaagcgtgaa ggaactgctg ggcatcacca ttatggagcg tagcagcttc
     4381 gaaaagaacc cgatcgactt tctggaggcg aaaggttaca aggaagtgaa gaaagatctg
     4441 atcatcaagc tgccgaagta tagcctgttt gagctggaaa atggtcgtaa acgtatgctg
     4501 gccagcgccg gcgagctgca aaagggcaac gaactggcgc tgccgagcaa atacgtgaac
     4561 ttcctgtatc tggccagcca ctacgagaag ctgaaaggta gcccggagga caacgaacag
     4621 aagcagctgt ttgtggagca gcacaaacac tacctggatg agatcattga acagattagc
     4681 gagttcagca agcgtgtgat cctggctgat gccaacctgg acaaagtgct gagcgcctat
     4741 aacaaacacc gtgacaagcc gatccgcgag caggcggaaa acatcattca cctgttcacc
     4801 ctgaccaacc tgggtgcgcc ggctgccttc aagtattttg ataccaccat tgaccgtaaa
     4861 cgctacacca gcaccaagga ggtgctggat gctaccctga tccaccagag cattaccggc
     4921 ctgtacgaaa cccgtatcga tctgagccag ctgggcggtg acccgaagaa aaagcgtaaa
     4981 gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac
     5041 ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagcatggat
     5101 agcctgctga tgaaccgtcg caaattcctg taccagttta agaacgtgcg ttgggctaaa
     5161 ggccgtcgcg aaacctatct gtgctacgtg gtgaaacgtc gcgatagcgc caccagcttc
     5221 agcctggact ttggttatct gcgcaacaag aacggctgcc acgtggagct gctgttcctg
     5281 cgttatatta gcgactggga cctggaccca ggccgttgct accgtgtgac ctggtttacc
     5341 agctggagcc cgtgctatga ttgcgcccgt catgtggctg acttcctgcg tggtaaccca
     5401 aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg taaggctgaa
     5461 ccagaaggtc tgcgtcgcct gcaccgtgct ggcgtgcaga tcgccatcat gaccttcaaa
     5521 gattactttt attgctggaa caccttcgtg gagaaccacg aacgcacctt taaggcctgg
     5581 gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgcat cctgctgcca
     5641 aagcgtccgg cggctaccaa gaaagccggt caggcgaaga aaaagaaaga ctacaaagat
     5701 gacgatgaca agggtagcgg cgctaccaac ttcagcctgc tgaagcaggc gggcgacgtg
     5761 gaggaaaatc cgggcccgat ggctaaaccg ctgagccagg aggaaagcac cctgatcgag
     5821 cgcgcgaccg ctaccattaa cagcatcccg attagcgaag attatagcgt ggccagcgcc
     5881 gcgctgagca gcgatggtcg tatcttcacc ggcgtgaacg tgtatcactt taccggtggc
     5941 ccgtgcgccg agctggtggt gctgggcacc gccgccgccg ccgccgccgg caacctgacc
     6001 tgcatcgtgg cgattggtaa cgaaaatcgt ggcatcctga gcccgtgcgg ccgttgccgc
     6061 caggtgctgc tggacctgca tccgggcatc aaggcgattg tgaaggatag cgacggccaa
     6121 ccgaccgctg tgggtattcg tgaactgctg ccatccggct atgtttggga aggctaaggt
     6181 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
     6241 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     6301 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6361 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6421 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6481 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6541 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6601 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6661 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6721 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6781 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6841 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6901 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6961 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     7021 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7081 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     7141 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     7201 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7261 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7321 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     7381 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     7441 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     7501 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7561 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7621 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7681 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7741 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7801 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7861 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7921 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7981 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     8041 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     8101 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     8161 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     8221 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8281 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8341 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8461 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8521 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8581 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8641 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8701 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8761 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8821 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8881 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8941 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9001 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9061 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9121 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9181 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9241 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9301 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9361 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9421 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9481 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9541 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9601 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9661 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9721 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9781 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9841 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9901 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9961 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10021 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10081 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10141 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10201 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10261 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10321 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10381 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10441 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10501 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10561 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10621 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10681 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
    10741 tctgctctga tgccgc
//
