LOCUS       sequence-267694-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: AIDx-ID-202
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        152..531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        532..735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     686..706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     780..799
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        780..798
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1446..1466
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1482..1502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5685..5732
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5733..5756
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5766..5822
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6327..6344)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6333..6557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6603..7031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6690..6709)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6900..6921
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7040..7060)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7045..7374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7225..7360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7287..7306
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7441..8235
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7495..7514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8105..8124
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8409..8530
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8446..8465)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8500..8519
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8579..8595)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8579..8595)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8592..8614)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8603..8619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8627..8633,8634..8651,8652..8657))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8672..8693
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8810..8827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8981..9569)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9061..9080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9740..10531,10532..10600))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10363..10382
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10601..10705)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10780..10799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
       61 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      121 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      181 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      241 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      301 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      361 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      421 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      481 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      541 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      601 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      661 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      721 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      781 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca
      841 tggataagaa atatagcatc ggcctggcaa tcggcaccaa tagcgtgggc tgggctgtga
      901 ttaccgacga atacaaagtt ccgtccaaga agttcaaggt gctgggtaac accgaccgcc
      961 acagcatcaa gaaaaacctg attggtgccc tgctgtttga tagcggtgaa accgccgagg
     1021 ctacccgtct gaagcgcacc gcccgtcgcc gttatacccg tcgtaaaaac cgtatctgct
     1081 acctgcaaga gattttcagc aacgaaatgg ccaaagtgga cgatagcttc tttcaccgcc
     1141 tggaggagag cttcctggtg gaggaagaca agaaacacga gcgtcacccg atctttggca
     1201 acattgtgga tgaggtggct tatcacgaaa agtacccgac catctatcac ctgcgtaaga
     1261 aactggtgga tagcaccgac aaggccgatc tgcgcctgat ctacctggcg ctggctcaca
     1321 tgatcaagtt ccgtggtcac tttctgatcg agggcgacct gaacccggat aacagcgatg
     1381 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgtttgag gaaaacccga
     1441 ttaacccgaa gaaaaagcgt aaagtgggta gcagcggcag cccgaaaaag aaacgcaaag
     1501 tgggtagcga cgccctggac gatttcgatc tggacatgct gggcagcgac gcgctggacg
     1561 attttggcgg tggcagcatg gatagcctgc tgatgaaccg ccgtaaattc ctgtaccagt
     1621 ttaagaacgt gcgttgggct aaaggtcgcc gtgaaaccta cctgtgctat gtggtgaaac
     1681 gccgtgatag cgccaccagc ttcagcctgg actttggtta tctgcgcaac aagaacggct
     1741 gccacgtgga gctgctgttc ctgcgttata tcagcgattg ggacctggac ccaggccgtt
     1801 gctaccgtgt gacctggttc accagctgga gcccgtgcta tgattgcgct cgtcacgtgg
     1861 ccgactttct gcgcggcaac ccgaacctga gcctgcgtat cttcaccgcg cgcctgtact
     1921 tttgcgagga ccgtaaggct gagccggaag gtctgcgccg tctgcaccgt gcgggtgtgc
     1981 agatcgcgat catgaccttc aaagattact tttattgctg gaacaccttc gtggagaacc
     2041 acgaacgtac ctttaaggcg tgggagggtc tgcacgaaaa cagcgtgcgt ctgagccgcc
     2101 agctgcgccg tatcctgctg ccgggtggca gcggtggcag cggtggcagc ggtggcagcg
     2161 gtggcagcgg tggcagcggt ggcaatgcca gcggtgtgga cgctaaggcc atcctgagcg
     2221 cgcgtctgag caaaagccgc cgtctggaga acctgattgc gcagctgccg ggtgaaaaga
     2281 aaaacggtct gtttggcaac ctgattgctc tgagcctggg cctgaccccg aacttcaaaa
     2341 gcaactttga cctggccgag gatgcgaagc tgcaactgag caaggatacc tacgacgatg
     2401 acctggacaa cctgctggcg cagatcggcg accagtatgc tgatctgttc ctggccgcga
     2461 agaacctgag cgatgcgatc ctgctgagcg acattctgcg tgtgaacacc gagattacca
     2521 aggcgccgct gagcgccagc atgatcaaac gctacgatga acaccaccag gacctgaccc
     2581 tgctgaaagc tctggtgcgt cagcagctgc cggagaagta taaagaaatc ttctttgacc
     2641 agagcaagaa cggttacgcc ggctatattg atggtggcgc gagccaggag gagttctaca
     2701 agtttatcaa accgattctg gagaagatgg acggcaccga ggaactgctg gtgaagctga
     2761 accgtgaaga tctgctgcgt aaacagcgca ccttcgacaa cggtagcatc ccgcaccaga
     2821 ttcacctggg tgagctgcac gccatcctgc gccgtcagga agatttctac ccgtttctga
     2881 aggacaaccg tgagaagatc gaaaaaattc tgaccttccg cattccgtac tatgtgggtc
     2941 cgctggctcg tggcaacagc cgctttgcct ggatgacccg caagagcgag gaaaccatca
     3001 ccccgtggaa cttcgaggaa gtggtggaca aaggcgccag cgcccagagc ttcattgagc
     3061 gtatgaccaa ctttgataag aacctgccga acgaaaaagt gctgccgaag cacagcctgc
     3121 tgtacgagta tttcaccgtg tataacgaac tgaccaaggt gaaatacgtg accgagggta
     3181 tgcgcaagcc ggccttcctg agcggcgaac agaagaaagc gatcgtggac ctgctgttta
     3241 aaaccaaccg taaggtgacc gtgaagcagc tgaaagagga ttacttcaag aaaattgaat
     3301 gcttcgacag cgtggagatc agcggtgtgg aagatcgttt taacgcgagc ctgggcacct
     3361 atcacgacct gctgaaaatc attaaggaca aagatttcct ggataacgag gaaaacgagg
     3421 acatcctgga agatattgtg ctgaccctga ccctgtttga ggaccgtgaa atgatcgagg
     3481 aacgcctgaa gacctacgcc cacctgttcg atgacaaagt gatgaaacag ctgaagcgcc
     3541 gtcgctatac cggttggggc cgtctgagcc gcaaactgat caacggtatt cgcgataaac
     3601 agagcggcaa gaccatcctg gattttctga agagcgacgg tttcgcgaac cgtaacttta
     3661 tgcagctgat tcacgatgac agcctgacct tcaaagagga catccagaag gcgcaggtga
     3721 gcggtcaggg cgatagcctg cacgaacaca tcgctaacct ggccggtagc ccggccatca
     3781 agaagggcat cctgcaaacc gtgaaggtgg tggacgagct ggtgaaagtg atgggtcgtc
     3841 acaaaccgga aaacatcgtg attgagatgg cccgcgaaaa ccagaccacc cagaaaggcc
     3901 agaagaacag ccgtgagcgc atgaagcgta tcgaggaagg catcaaggaa ctgggcagcc
     3961 agatcctgaa ggagcacccg gtggaaaaca cccagctgca aaacgagaaa ctgtatctgt
     4021 actacctgca aaacggtcgt gacatgtacg tggatcagga gctggacatt aaccgcctga
     4081 gcgactacga tgtggacgcg atcgtgccgc agagcttcct gaaggatgac agcattgaca
     4141 acaaagtgct gacccgtagc gataaaaacc gcggcaagag cgacaacgtg ccgagcgagg
     4201 aagtggtgaa gaaaatgaaa aactactggc gtcagctgct gaacgccaag ctgatcaccc
     4261 agcgtaaatt tgacaacctg accaaggctg agcgcggtgg cctgagcgaa ctggataaag
     4321 ccggtttcat taagcgtcag ctggtggaaa cccgccagat caccaagcac gtggcgcaga
     4381 ttctggacag ccgcatgaac accaaatacg acgagaacga taagctgatc cgtgaagtga
     4441 aagtgattac cctgaagagc aaactggtga gcgattttcg taaggacttc cagttttaca
     4501 aagtgcgcga gatcaacaac tatcaccacg cgcacgacgc ttacctgaac gctgtggtgg
     4561 gcaccgccct gatcaagaag tatccgaagc tggagagcga gttcgtgtac ggcgattata
     4621 aagtgtacga cgtgcgtaaa atgatcgcca agagcgagca ggaaattggc aaagcgaccg
     4681 ctaagtattt cttttacagc aacatcatga acttctttaa gaccgagatc accctggcca
     4741 acggtgaaat ccgtaaacgc ccgctgattg aaaccaacgg tgaaaccggc gaaatcgtgt
     4801 gggataaggg ccgtgacttt gcgaccgtgc gcaaagtgct gtccatgccg caggtgaaca
     4861 ttgtgaagaa aaccgaggtg cagaccggtg gcttcagcaa ggaaagcatt ctgccgaaac
     4921 gtaacagcga caagctgatc gcccgcaaga aagattggga cccgaagaaa tatggtggct
     4981 tcgatagccc aaccgtggcc tacagcgtgc tggtggtggc taaagtggag aagggtaaaa
     5041 gcaagaaact gaaaagcgtg aaggaactgc tgggcatcac cattatggag cgtagcagct
     5101 tcgaaaagaa cccgatcgac tttctggagg cgaaaggtta caaggaagtg aagaaagatc
     5161 tgatcatcaa gctgccgaag tatagcctgt ttgagctgga aaatggtcgt aaacgtatgc
     5221 tggccagcgc cggcgagctg caaaagggca acgaactggc gctgccgagc aaatacgtga
     5281 acttcctgta tctggccagc cactacgaga agctgaaagg tagcccggag gacaacgaac
     5341 agaagcagct gtttgtggag cagcacaaac actacctgga tgagatcatt gaacagatta
     5401 gcgagttcag caagcgtgtg atcctggctg atgccaacct ggacaaagtg ctgagcgcct
     5461 ataacaaaca ccgtgacaag ccgatccgcg agcaggccga aaacatcatt cacctgttta
     5521 ccctgaccaa cctgggcgcg ccggctgcct tcaaatattt tgataccacc attgaccgta
     5581 agcgttacac cagcaccaaa gaggtgctgg atgctaccct gatccaccag agcattaccg
     5641 gtctgtacga aacccgtatc gatctgagcc agctgggtgg cgacaagcgc ccggcggcta
     5701 ccaagaaagc cggtcaggcg aagaaaaaga aagactacaa ggatgacgat gacaagggta
     5761 gcggcgctac caacttcagc ctgctgaagc aggcgggcga cgtggaggaa aatccgggcc
     5821 cgatggctaa accgctgagc caggaggaaa gcaccctgat cgagcgcgcg accgctacca
     5881 ttaacagcat cccgattagc gaagattata gcgtggccag cgccgcgctg agcagcgatg
     5941 gtcgtatctt caccggcgtg aacgtgtatc actttaccgg tggcccgtgc gccgagctgg
     6001 tggtgctggg caccgccgcc gccgccgccg ccggcaacct gacctgcatc gtggcgattg
     6061 gtaacgaaaa tcgtggcatc ctgagcccgt gcggccgttg ccgccaggtg ctgctggacc
     6121 tgcatccggg catcaaggcg attgtgaagg atagcgacgg ccaaccgacc gccgtgggta
     6181 ttcgtgaact gctgccatcc ggctatgttt gggaaggcta aggtaccgag ctcggatcca
     6241 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     6301 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6541 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6601 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6961 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7021 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7081 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7141 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7201 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     7321 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7381 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     7441 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     7501 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     7561 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     7621 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     7681 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     7741 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     7801 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     7861 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     7921 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     7981 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     8041 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     8101 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     8161 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     8221 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     8281 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     8341 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     8401 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8461 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8521 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     8581 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     8641 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     8701 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     8761 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     8881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     8941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     9721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     9781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     9841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     9901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    10021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    10081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    10141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    10201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    10261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    10321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    10381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    10441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    10501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    10561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    10621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    10681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    10741 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
    10801 atagttaagc cagtatctg
//
