LOCUS       sequence-267695-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 208 of nSpyCas9 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: AIDx-ID-208
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        121..500
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        501..704
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     655..675
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     749..768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      803..812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1433..1453
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1469..1489
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5654..5701
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5702..5725
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5735..5791
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6296..6313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6302..6526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6572..7000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6659..6678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6869..6890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7009..7029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7014..7343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7194..7329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7256..7275
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7410..8204
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7464..7483)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8074..8093
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8378..8499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8415..8434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8469..8488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8548..8564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8548..8564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8561..8583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8572..8588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8596..8602,8603..8620,8621..8626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8641..8662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8779..8796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8950..9538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9030..9049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9709..10500,10501..10569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10332..10351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10570..10674)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10749..10768)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
       61 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      121 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      181 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      241 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      301 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      361 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      421 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      481 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      541 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      601 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      661 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      721 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      781 gctagcgttt aaacttaagc ttgccaccat ggataagaaa tatagcatcg gcctggcaat
      841 cggcaccaat agcgtgggct gggctgtgat taccgacgaa tacaaagttc cgtccaagaa
      901 gttcaaggtg ctgggtaaca ccgaccgcca cagcatcaag aaaaacctga ttggtgccct
      961 gctgtttgat agcggtgaaa ccgccgaggc tacccgtctg aagcgcaccg cccgtcgccg
     1021 ttatacccgt cgtaaaaacc gtatctgcta cctgcaagag attttcagca acgaaatggc
     1081 caaagtggac gatagcttct ttcaccgcct ggaggagagc ttcctggtgg aggaagacaa
     1141 gaaacacgag cgtcacccga tctttggcaa cattgtggat gaggtggctt atcacgaaaa
     1201 gtacccgacc atctatcacc tgcgtaagaa actggtggat agcaccgaca aggccgatct
     1261 gcgcctgatc tacctggcgc tggctcacat gatcaagttc cgtggtcact ttctgatcga
     1321 gggcgacctg aacccggata acagcgatgt ggacaagctg ttcatccagc tggtgcagac
     1381 ctacaaccag ctgtttgagg aaaacccgat taacgccagc ggtgtggacg ctccgaagaa
     1441 aaagcgtaaa gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc
     1501 cctggacgat ttcgatctgg acatgctggg cagcgacgcg ctggacgatt ttggcggtgg
     1561 cagcatggat agcctgctga tgaaccgccg taaattcctg taccagttta agaacgtgcg
     1621 ttgggctaaa ggtcgccgtg aaacctacct gtgctatgtg gtgaaacgcc gtgatagcgc
     1681 caccagcttc agcctggact ttggttatct gcgcaacaag aacggctgcc acgtggagct
     1741 gctgttcctg cgttatatca gcgattggga cctggaccca ggccgttgct accgtgtgac
     1801 ctggttcacc agctggagcc cgtgctatga ttgcgctcgt cacgtggccg actttctgcg
     1861 cggcaacccg aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg
     1921 taaggctgag ccggaaggtc tgcgccgtct gcaccgtgcg ggtgtgcaga tcgcgatcat
     1981 gaccttcaaa gattactttt attgctggaa caccttcgtg gagaaccacg aacgtacctt
     2041 taaggcgtgg gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgtat
     2101 cctgctgccg ggtggcagcg gtggcagcgg tggcagcggt ggcagcggtg gcagcggtgg
     2161 cagcggtggc gctaaggcca tcctgagcgc gcgtctgagc aaaagccgcc gtctggagaa
     2221 cctgattgcg cagctgccgg gtgaaaagaa aaacggtctg tttggcaacc tgattgctct
     2281 gagcctgggc ctgaccccga acttcaaaag caactttgac ctggccgagg atgcgaagct
     2341 gcaactgagc aaggatacct acgacgatga cctggacaac ctgctggcgc agatcggcga
     2401 ccagtatgct gatctgttcc tggccgcgaa gaacctgagc gatgcgatcc tgctgagcga
     2461 cattctgcgt gtgaacaccg agattaccaa ggcgccgctg agcgccagca tgatcaaacg
     2521 ctacgatgaa caccaccagg acctgaccct gctgaaagct ctggtgcgtc agcagctgcc
     2581 ggagaagtat aaagaaatct tctttgacca gagcaagaac ggttacgccg gctatattga
     2641 tggtggcgcg agccaggagg agttctacaa gtttatcaaa ccgattctgg agaagatgga
     2701 cggcaccgag gaactgctgg tgaagctgaa ccgtgaagat ctgctgcgta aacagcgcac
     2761 cttcgacaac ggtagcatcc cgcaccagat tcacctgggt gagctgcacg ccatcctgcg
     2821 ccgtcaggaa gatttctacc cgtttctgaa ggacaaccgt gagaagatcg aaaaaattct
     2881 gaccttccgc attccgtact atgtgggtcc gctggctcgt ggcaacagcc gctttgcctg
     2941 gatgacccgc aagagcgagg aaaccatcac cccgtggaac ttcgaggaag tggtggacaa
     3001 aggcgccagc gcccagagct tcattgagcg tatgaccaac tttgataaga acctgccgaa
     3061 cgaaaaagtg ctgccgaagc acagcctgct gtacgagtat ttcaccgtgt ataacgaact
     3121 gaccaaggtg aaatacgtga ccgagggtat gcgcaagccg gccttcctga gcggcgaaca
     3181 gaagaaagcg atcgtggacc tgctgtttaa aaccaaccgt aaggtgaccg tgaagcagct
     3241 gaaagaggat tacttcaaga aaattgaatg cttcgacagc gtggagatca gcggtgtgga
     3301 agatcgtttt aacgcgagcc tgggcaccta tcacgacctg ctgaaaatca ttaaggacaa
     3361 agatttcctg gataacgagg aaaacgagga catcctggaa gatattgtgc tgaccctgac
     3421 cctgtttgag gaccgtgaaa tgatcgagga acgcctgaag acctacgccc acctgttcga
     3481 tgacaaagtg atgaaacagc tgaagcgccg tcgctatacc ggttggggcc gtctgagccg
     3541 caaactgatc aacggtattc gcgataaaca gagcggcaag accatcctgg attttctgaa
     3601 gagcgacggt ttcgcgaacc gtaactttat gcagctgatt cacgatgaca gcctgacctt
     3661 caaagaggac atccagaagg cgcaggtgag cggtcagggc gatagcctgc acgaacacat
     3721 cgctaacctg gccggtagcc cggccatcaa gaagggcatc ctgcaaaccg tgaaggtggt
     3781 ggacgagctg gtgaaagtga tgggtcgtca caaaccggaa aacatcgtga ttgagatggc
     3841 ccgcgaaaac cagaccaccc agaaaggcca gaagaacagc cgtgagcgca tgaagcgtat
     3901 cgaggaaggc atcaaggaac tgggcagcca gatcctgaag gagcacccgg tggaaaacac
     3961 ccagctgcaa aacgagaaac tgtatctgta ctacctgcaa aacggtcgtg acatgtacgt
     4021 ggatcaggag ctggacatta accgcctgag cgactacgat gtggacgcga tcgtgccgca
     4081 gagcttcctg aaggatgaca gcattgacaa caaagtgctg acccgtagcg ataaaaaccg
     4141 cggcaagagc gacaacgtgc cgagcgagga agtggtgaag aaaatgaaaa actactggcg
     4201 tcagctgctg aacgccaagc tgatcaccca gcgtaaattt gacaacctga ccaaggctga
     4261 gcgcggtggc ctgagcgaac tggataaagc cggtttcatt aagcgtcagc tggtggaaac
     4321 ccgccagatc accaagcacg tggcgcagat tctggacagc cgcatgaaca ccaaatacga
     4381 cgagaacgat aagctgatcc gtgaagtgaa agtgattacc ctgaagagca aactggtgag
     4441 cgattttcgt aaggacttcc agttttacaa agtgcgcgag atcaacaact atcaccacgc
     4501 gcacgacgct tacctgaacg ctgtggtggg caccgccctg atcaagaagt atccgaagct
     4561 ggagagcgag ttcgtgtacg gcgattataa agtgtacgac gtgcgtaaaa tgatcgccaa
     4621 gagcgagcag gaaattggca aagcgaccgc taagtatttc ttttacagca acatcatgaa
     4681 cttctttaag accgagatca ccctggccaa cggtgaaatc cgtaaacgcc cgctgattga
     4741 aaccaacggt gaaaccggcg aaatcgtgtg ggataagggc cgtgactttg cgaccgtgcg
     4801 caaagtgctg tccatgccgc aggtgaacat tgtgaagaaa accgaggtgc agaccggtgg
     4861 cttcagcaag gaaagcattc tgccgaaacg taacagcgac aagctgatcg cccgcaagaa
     4921 agattgggac ccgaagaaat atggtggctt cgatagccca accgtggcct acagcgtgct
     4981 ggtggtggct aaagtggaga agggtaaaag caagaaactg aaaagcgtga aggaactgct
     5041 gggcatcacc attatggagc gtagcagctt cgaaaagaac ccgatcgact ttctggaggc
     5101 gaaaggttac aaggaagtga agaaagatct gatcatcaag ctgccgaagt atagcctgtt
     5161 tgagctggaa aatggtcgta aacgtatgct ggccagcgcc ggcgagctgc aaaagggcaa
     5221 cgaactggcg ctgccgagca aatacgtgaa cttcctgtat ctggccagcc actacgagaa
     5281 gctgaaaggt agcccggagg acaacgaaca gaagcagctg tttgtggagc agcacaaaca
     5341 ctacctggat gagatcattg aacagattag cgagttcagc aagcgtgtga tcctggctga
     5401 tgccaacctg gacaaagtgc tgagcgccta taacaaacac cgtgacaagc cgatccgcga
     5461 gcaggccgaa aacatcattc acctgtttac cctgaccaac ctgggcgcgc cggctgcctt
     5521 caaatatttt gataccacca ttgaccgtaa gcgttacacc agcaccaaag aggtgctgga
     5581 tgctaccctg atccaccaga gcattaccgg tctgtacgaa acccgtatcg atctgagcca
     5641 gctgggtggc gacaagcgcc cggcggctac caagaaagcc ggtcaggcga agaaaaagaa
     5701 agactacaag gatgacgatg acaagggtag cggcgctacc aacttcagcc tgctgaagca
     5761 ggcgggcgac gtggaggaaa atccgggccc gatggctaaa ccgctgagcc aggaggaaag
     5821 caccctgatc gagcgcgcga ccgctaccat taacagcatc ccgattagcg aagattatag
     5881 cgtggccagc gccgcgctga gcagcgatgg tcgtatcttc accggcgtga acgtgtatca
     5941 ctttaccggt ggcccgtgcg ccgagctggt ggtgctgggc accgccgccg ccgccgccgc
     6001 cggcaacctg acctgcatcg tggcgattgg taacgaaaat cgtggcatcc tgagcccgtg
     6061 cggccgttgc cgccaggtgc tgctggacct gcatccgggc atcaaggcga ttgtgaagga
     6121 tagcgacggc caaccgaccg ccgtgggtat tcgtgaactg ctgccatccg gctatgtttg
     6181 ggaaggctaa ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
     6241 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     6301 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6361 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6421 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6481 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6541 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6601 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6661 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6721 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6781 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6841 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6901 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6961 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7021 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7081 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7141 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7201 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7261 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7321 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7381 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7441 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7501 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7561 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7621 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7681 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7741 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7801 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7861 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7921 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7981 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8041 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8101 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8161 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8221 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8281 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8341 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8401 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8461 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     8521 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9361 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10681 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    10741 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    10801 gtgttggagg tcgctgagt
//
