LOCUS       sequence-267696-       11080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 1058 of nSpyCas9 with uracil DNA 
            glycosylase inhibitor (UGI) at the C terminus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: AIDx-ID-1058
FEATURES             Location/Qualifiers
     source          1..11080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     725..744
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        725..743
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3959..3979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3995..4015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5642..5890
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5891..5938
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5939..5962
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5972..6028
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6533..6550)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6539..6763
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6809..7237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6896..6915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7106..7127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7246..7266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7251..7580
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7431..7566
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7493..7512
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7647..8441
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7701..7720)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8311..8330
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8615..8736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8652..8671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8706..8725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8785..8801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8785..8801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8798..8820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8809..8825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8833..8839,8840..8857,8858..8863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8878..8899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9016..9033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9187..9775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9267..9286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9946..10737,10738..10806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10569..10588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10807..10911)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10986..11005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
       61 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      721 aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc
      781 caccatggat aaaaaatata gcattggtct ggcgattggc accaactccg tgggctgggc
      841 tgtgattacc gatgaataca aggttccgag caagaagttt aaggtgctgg gtaacaccga
      901 ccgtcacagc atcaagaaga acctgattgg cgctctgctg tttgacagcg gcgagaccgc
      961 ggaagccacc cgcctgaagc gcaccgcgcg tcgccgttat acccgtcgta agaaccgcat
     1021 ttgctatctg caagaaatct tcagcaacga gatggcgaaa gtggatgaca gctttttcca
     1081 ccgcctggaa gagagctttc tggtggagga agataagaag cacgagcgtc acccgatctt
     1141 cggtaacatt gtggacgagg tggcctatca cgaaaagtat ccgaccatct accacctgcg
     1201 taaaaaactg gtggatagca ccgacaaagc tgatctgcgc ctgatctatc tggctctggc
     1261 gcacatgatc aaattccgcg gccacttcct gattgagggt gacctgaacc cggacaacag
     1321 cgatgtggat aaactgttta ttcagctggt gcagacctat aaccagctgt ttgaagagaa
     1381 cccgatcaac gcgagcggcg tggacgcgaa agctattctg agcgcgcgtc tgagcaagag
     1441 ccgtcgtctg gaaaacctga tcgcgcagct gccgggcgag aagaaaaacg gtctgtttgg
     1501 caacctgatt gccctgagcc tgggtctgac cccgaacttt aagagcaact tcgacctggc
     1561 ggaggacgcg aaactgcaac tgagcaaaga tacctatgac gacgacctgg ataacctgct
     1621 ggcgcagatc ggcgatcagt atgctgacct gtttctggcg gccaaaaacc tgagcgacgc
     1681 gattctgctg agcgacatcc tgcgtgtgaa caccgagatt accaaagcgc cgctgagcgc
     1741 cagcatgatt aagcgttacg acgaacacca ccaggacctg accctgctga aagccctggt
     1801 gcgccagcag ctgccggaga agtacaaaga aatctttttc gatcagagca aaaacggtta
     1861 tgccggttat attgacggtg gcgcgagcca ggaggagttt tataagttca ttaagccgat
     1921 tctggaaaag atggacggca ccgaggagct gctggtgaag ctgaaccgcg aagacctgct
     1981 gcgcaaacag cgtacctttg ataacggtag cattccgcac cagattcacc tgggcgaact
     2041 gcacgcgatt ctgcgtcgcc aggaggactt ctatccgttc ctgaaggata accgtgagaa
     2101 aattgagaag attctgacct tccgtatccc gtactatgtg ggcccgctgg ctcgtggtaa
     2161 cagccgtttc gcttggatga cccgtaaaag cgaggagacc attaccccgt ggaacttcga
     2221 ggaagtggtg gacaaaggtg ccagcgcgca gagctttatc gagcgcatga ccaacttcga
     2281 caaaaacctg ccgaacgaaa aagtgctgcc gaaacacagc ctgctgtatg aatacttcac
     2341 cgtgtataac gaactgacca aggtgaagta tgtgaccgaa ggtatgcgca agccggcttt
     2401 tctgagcggc gagcagaaga aggcgattgt ggacctgctg ttcaagacca accgtaaagt
     2461 gaccgtgaag cagctgaagg aggactactt caagaaaatt gaatgctttg acagcgtgga
     2521 gatcagcggc gtggaggacc gcttcaacgc gagcctgggc acctaccacg atctgctgaa
     2581 gatcattaag gataaagatt ttctggacaa cgaggaaaac gaagacatcc tggaggatat
     2641 tgtgctgacc ctgaccctgt ttgaggatcg tgaaatgatt gaagaacgcc tgaagaccta
     2701 tgctcacctg ttcgatgata aagtgatgaa acagctgaag cgtcgtcgtt ataccggttg
     2761 gggccgtctg agccgcaaac tgatcaacgg cattcgtgac aaacagagcg gcaagaccat
     2821 cctggatttt ctgaaaagcg atggctttgc gaaccgtaac tttatgcagc tgattcacga
     2881 tgatagcctg accttcaagg aggacattca gaaggcgcag gtgagcggcc agggtgacag
     2941 cctgcacgag cacattgcga acctggcggg cagcccggcg attaagaaag gcattctgca
     3001 aaccgtgaaa gtggtggatg aactggtgaa agtgatgggc cgccacaagc cggagaacat
     3061 tgtgatcgag atggcccgtg agaaccagac cacccagaaa ggccagaaga acagccgcga
     3121 gcgcatgaag cgtattgaag agggtatcaa ggagctgggc agccagatcc tgaaggaaca
     3181 cccggtggaa aacacccagc tgcaaaacga gaaactgtat ctgtactacc tgcaaaacgg
     3241 ccgtgacatg tatgtggatc aggaactgga catcaaccgc ctgagcgact acgacgtgga
     3301 tgcgatcgtg ccgcagagct tcctgaaaga cgacagcatt gataacaagg tgctgacccg
     3361 tagcgacaag aaccgtggca aaagcgataa cgtgccgagc gaggaggtgg tgaaaaagat
     3421 gaaaaactat tggcgccagc tgctgaacgc gaagctgatt acccagcgca agttcgacaa
     3481 cctgaccaag gctgagcgtg gtggcctgag cgagctggac aaggctggct ttatcaaacg
     3541 ccagctggtg gagacccgcc agatcaccaa gcacgtggcg cagatcctgg atagccgtat
     3601 gaacaccaaa tacgatgaga acgataagct gatccgcgag gtgaaagtga tcaccctgaa
     3661 gagcaagctg gtgagcgatt ttcgcaagga cttccagttc tacaaggtgc gcgaaatcaa
     3721 caactaccac cacgcgcacg atgcgtatct gaacgcggtg gtgggcaccg ctctgatcaa
     3781 gaaatatccg aagctggaga gcgagttcgt gtacggtgac tataaagtgt acgacgtgcg
     3841 caaaatgatt gccaagagcg agcaggagat tggtaaagct accgctaaat atttctttta
     3901 cagcaacatc atgaacttct ttaagaccga gatcaccctg gccaacggtg aaatccgtcc
     3961 gaagaagaaa cgtaaggtgg gtagcagcgg tagcccgaaa aagaagcgca aagtgggcag
     4021 cgacgcgctg gacgatttcg atctggacat gctgggcagc gatgcgctgg atgatttcgg
     4081 cggtggcagc atggatagcc tgctgatgaa ccgtcgcaag ttcctgtacc agttcaaaaa
     4141 cgtgcgttgg gcgaagggtc gtcgtgagac ctacctgtgc tacgtggtga agcgtcgtga
     4201 cagcgcgacc agcttcagcc tggattttgg ctatctgcgt aacaaaaacg gttgccacgt
     4261 ggaactgctg tttctgcgct acattagcga ctgggacctg gaccccggtc gctgctaccg
     4321 cgtgacctgg tttaccagct ggagcccgtg ctatgattgc gcgcgtcacg tggccgattt
     4381 cctgcgcggt aacccgaacc tgagcctgcg cattttcacc gcgcgcctgt atttctgcga
     4441 ggatcgcaag gcggagccgg agggcctgcg tcgtctgcac cgtgctggtg tgcagattgc
     4501 gattatgacc tttaaggact acttttattg ctggaacacc tttgtggaga accacgagcg
     4561 caccttcaaa gcgtgggagg gtctgcacga gaacagcgtg cgtctgagcc gccagctgcg
     4621 tcgcatcctg ctgccgggcg gtagcggcgg tagcggtggt agcggcggca gcggtggtag
     4681 cggtggcagc ggtggtcgca aacgcccgct gatcgaaacc aacggcgaaa ccggtgaaat
     4741 cgtgtgggat aagggtcgcg atttcgcgac cgtgcgcaag gtgctgagca tgccgcaggt
     4801 gaacatcgtg aagaagaccg aagtgcagac cggcggtttt agcaaggaaa gcatcctgcc
     4861 gaagcgcaac agcgataagc tgatcgcgcg taagaaggac tgggacccga aaaagtatgg
     4921 tggcttcgac agcccgaccg tggcgtacag cgtgctggtg gtggccaagg tggaaaaggg
     4981 caaaagcaaa aaactgaaaa gcgtgaagga gctgctgggt attaccatta tggaacgtag
     5041 cagctttgag aagaacccga tcgacttcct ggaggcgaag ggttacaagg aagtgaagaa
     5101 agatctgatc atcaaactgc cgaagtacag cctgtttgag ctggaaaacg gccgtaagcg
     5161 tatgctggcg agcgcgggtg agctgcaaaa aggtaacgag ctggcgctgc cgagcaagta
     5221 cgtgaacttt ctgtacctgg cgagccacta tgaaaaactg aaaggcagcc cggaggacaa
     5281 cgagcagaag cagctgtttg tggagcagca caagcactat ctggacgaaa tcatcgaaca
     5341 gatcagcgag ttcagcaaac gcgtgattct ggccgacgcc aacctggaca aagtgctgag
     5401 cgcctacaac aagcaccgcg ataaaccgat tcgcgaacag gccgagaaca tcattcacct
     5461 gtttaccctg accaacctgg gcgcgccggc ggcgttcaaa tactttgata ccaccattga
     5521 tcgcaaacgc tacaccagca ccaaggaagt gctggatgcg accctgatcc accagagcat
     5581 taccggtctg tacgaaaccc gcatcgatct gagccagctg ggcggcgata gcggtggtag
     5641 caccaacctg agcgacatca ttgagaaaga gaccggcaag cagctggtaa tccaggaaag
     5701 cattctgatg ctgccggaag aagtggagga agtgattggt aacaaaccgg aaagcgacat
     5761 cctggtgcac accgcttatg acgagagcac cgacgaaaac gtgatgctgc tgaccagcga
     5821 cgcgccggag tataagccgt gggcgctggt aattcaggat agcaacggcg aaaacaaaat
     5881 caagatgctg aagcgtccgg cggcgaccaa aaaggccggc caggcgaaga agaagaagga
     5941 ctacaaggat gacgatgaca aaggcagcgg cgccaccaac ttcagcctgc tgaagcaggc
     6001 cggtgacgtg gaagaaaacc ccggcccgat ggcgaagccg ctgagccagg aggaaagcac
     6061 cctgattgag cgcgccaccg cgaccatcaa cagcattccg attagcgaag actacagcgt
     6121 ggcgagcgcc gctctgagca gcgatggccg tatcttcacc ggcgtgaacg tgtaccactt
     6181 taccggcggt ccgtgcgcgg aactggtggt gctgggcacc gcggcggctg cggctgcggg
     6241 taacctgacc tgcatcgtgg ccattggtaa cgaaaaccgc ggcattctga gcccgtgcgg
     6301 ccgttgccgc caggtgctgc tggacctgca ccccggtatc aaagcgattg tgaaagatag
     6361 cgatggtcaa ccgaccgctg tgggtatccg tgaactgctg ccatccggct atgtttggga
     6421 gggctaaggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca
     6481 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     6541 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6601 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6661 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6721 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     6781 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     6841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     6901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7201 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     7261 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7321 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7381 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7441 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7501 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7561 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     7621 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     7681 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     7741 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     7801 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     7861 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     7921 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     7981 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     8041 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     8101 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     8161 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     8221 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     8281 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     8341 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     8401 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     8461 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     8521 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     8581 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     8641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8701 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     8761 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8821 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8881 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8941 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     9001 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9061 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9121 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9181 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9241 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9301 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9361 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9421 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9481 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9541 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9601 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     9661 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9721 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9781 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9841 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9901 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9961 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    10021 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    10081 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    10141 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    10201 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    10261 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    10321 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10381 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10441 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10501 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10561 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10621 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10681 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10741 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10801 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10861 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10921 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
    10981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
    11041 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
//
