LOCUS       sequence-267699-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of rat cytosine deaminase, 
            rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA 
            glycosylase inhibitor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 APOBEC3 no UGI
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(19..38)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        210..589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        590..793
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     744..764
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     838..857
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        838..856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      892..901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4072..4092
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4108..4128
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4204..4890
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             5896..5943
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5944..5967
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5977..6033
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6538..6555)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6544..6768
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6814..7242
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6901..6920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7111..7132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7251..7271)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7256..7585
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7436..7571
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7498..7517
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7652..8446
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7706..7725)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8316..8335
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8620..8741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8657..8676)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8711..8730
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8790..8806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8790..8806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8803..8825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8814..8830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8838..8844,8845..8862,8863..8868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8883..8904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9021..9038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9192..9780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9272..9291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9951..10742,10743..10811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10574..10593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10812..10916)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
       61 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      121 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      181 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      421 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      481 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      541 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      601 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      661 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      721 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      781 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
      841 tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct tgccaccatg
      901 gataaaaaat atagcattgg tctggcgatt ggcaccaact ccgtgggctg ggctgtgatt
      961 accgatgaat acaaggttcc gagcaagaag tttaaggtgc tgggtaacac cgaccgtcac
     1021 agcatcaaga agaacctgat tggcgctctg ctgtttgaca gcggcgagac cgcggaagcc
     1081 acccgcctga agcgcaccgc gcgtcgccgt tatacccgtc gtaagaaccg catttgctat
     1141 ctgcaagaaa tcttcagcaa cgagatggcg aaagtggatg acagcttttt ccaccgcctg
     1201 gaagagagct ttctggtgga ggaagataag aagcacgagc gtcacccgat cttcggtaac
     1261 attgtggacg aggtggccta tcacgaaaag tatccgacca tctaccacct gcgtaaaaaa
     1321 ctggtggata gcaccgacaa agctgatctg cgcctgatct atctggctct ggcgcacatg
     1381 atcaaattcc gcggccactt cctgattgag ggtgacctga acccggacaa cagcgatgtg
     1441 gataaactgt ttattcagct ggtgcagacc tataaccagc tgtttgaaga gaacccgatc
     1501 aacgcgagcg gcgtggacgc gaaagctatt ctgagcgcgc gtctgagcaa gagccgtcgt
     1561 ctggaaaacc tgatcgcgca gctgccgggc gagaagaaaa acggtctgtt tggcaacctg
     1621 attgccctga gcctgggtct gaccccgaac tttaagagca acttcgacct ggcggaggac
     1681 gcgaaactgc aactgagcaa agatacctat gacgacgacc tggataacct gctggcgcag
     1741 atcggcgatc agtatgctga cctgtttctg gcggccaaaa acctgagcga cgcgattctg
     1801 ctgagcgaca tcctgcgtgt gaacaccgag attaccaaag cgccgctgag cgccagcatg
     1861 attaagcgtt acgacgaaca ccaccaggac ctgaccctgc tgaaagccct ggtgcgccag
     1921 cagctgccgg agaagtacaa agaaatcttt ttcgatcaga gcaaaaacgg ttatgccggt
     1981 tatattgacg gtggcgcgag ccaggaggag ttttataagt tcattaagcc gattctggaa
     2041 aagatggacg gcaccgagga gctgctggtg aagctgaacc gcgaagacct gctgcgcaaa
     2101 cagcgtacct ttgataacgg tagcattccg caccagattc acctgggcga actgcacgcg
     2161 attctgcgtc gccaggagga cttctatccg ttcctgaagg ataaccgtga gaaaattgag
     2221 aagattctga ccttccgtat cccgtactat gtgggcccgc tggctcgtgg taacagccgt
     2281 ttcgcttgga tgacccgtaa aagcgaggag accattaccc cgtggaactt cgaggaagtg
     2341 gtggacaaag gtgccagcgc gcagagcttt atcgagcgca tgaccaactt cgacaaaaac
     2401 ctgccgaacg aaaaagtgct gccgaaacac agcctgctgt atgaatactt caccgtgtat
     2461 aacgaactga ccaaggtgaa gtatgtgacc gaaggtatgc gcaagccggc ttttctgagc
     2521 ggcgagcaga agaaggcgat tgtggacctg ctgttcaaga ccaaccgtaa agtgaccgtg
     2581 aagcagctga aggaggacta cttcaagaaa attgaatgct ttgacagcgt ggagatcagc
     2641 ggcgtggagg accgcttcaa cgcgagcctg ggcacctacc acgatctgct gaagatcatt
     2701 aaggataaag attttctgga caacgaggaa aacgaagaca tcctggagga tattgtgctg
     2761 accctgaccc tgtttgagga tcgtgaaatg attgaagaac gcctgaagac ctatgctcac
     2821 ctgttcgatg ataaagtgat gaaacagctg aagcgtcgtc gttataccgg ttggggccgt
     2881 ctgagccgca aactgatcaa cggcattcgt gacaaacaga gcggcaagac catcctggat
     2941 tttctgaaaa gcgatggctt tgcgaaccgt aactttatgc agctgattca cgatgatagc
     3001 ctgaccttca aggaggacat tcagaaggcg caggtgagcg gccagggtga cagcctgcac
     3061 gagcacattg cgaacctggc gggcagcccg gcgattaaga aaggcattct gcaaaccgtg
     3121 aaagtggtgg atgaactggt gaaagtgatg ggccgccaca agccggagaa cattgtgatc
     3181 gagatggccc gtgagaacca gaccacccag aaaggccaga agaacagccg cgagcgcatg
     3241 aagcgtattg aagagggtat caaggagctg ggcagccaga tcctgaagga acacccggtg
     3301 gaaaacaccc agctgcaaaa cgagaaactg tatctgtact acctgcaaaa cggccgtgac
     3361 atgtatgtgg atcaggaact ggacatcaac cgcctgagcg actacgacgt ggatgcgatc
     3421 gtgccgcaga gcttcctgaa agacgacagc attgataaca aggtgctgac ccgtagcgac
     3481 aagaaccgtg gcaaaagcga taacgtgccg agcgaggagg tggtgaaaaa gatgaaaaac
     3541 tattggcgcc agctgctgaa cgcgaagctg attacccagc gcaagttcga caacctgacc
     3601 aaggctgagc gtggtggcct gagcgagctg gacaaggctg gctttatcaa acgccagctg
     3661 gtggagaccc gccagatcac caagcacgtg gcgcagatcc tggatagccg tatgaacacc
     3721 aaatacgatg agaacgataa gctgatccgc gaggtgaaag tgatcaccct gaagagcaag
     3781 ctggtgagcg attttcgcaa ggacttccag ttctacaagg tgcgcgaaat caacaactac
     3841 caccacgcgc acgatgcgta tctgaacgcg gtggtgggca ccgctctgat caagaaatat
     3901 ccgaagctgg agagcgagtt cgtgtacggt gactataaag tgtacgacgt gcgcaaaatg
     3961 attgccaaga gcgagcagga gattggtaaa gctaccgcta aatatttctt ttacagcaac
     4021 atcatgaact tctttaagac cgagatcacc ctggccaacg gtgaaatccg tccgaagaag
     4081 aaacgtaagg tgggtagcag cggtagcccg aaaaagaagc gcaaagtggg cagcgacgcg
     4141 ctggacgatt tcgatctgga catgctgggc agcgatgcgc tggatgattt cggcggtggc
     4201 agcatgagct cagagactgg cccagtggct gtggacccca cattgagacg gcggatcgag
     4261 ccccatgagt ttgaggtatt cttcgatccg agagagctcc gcaaggagac ctgcctgctt
     4321 tacgaaatta attggggggg ccggcactcc atttggcgac atacatcaca gaacactaac
     4381 aagcacgtcg aagtcaactt catcgagaag ttcacgacag aaagatattt ctgtccgaac
     4441 acaaggtgca gcattacctg gtttctcagc tggagcccat gcggcgaatg tagtagggcc
     4501 atcactgaat tcctgtcaag gtatccccac gtcactctgt ttatttacat cgcaaggctg
     4561 taccaccacg ctgacccccg caatcgacaa ggcctgcggg atttgatctc ttcaggtgtg
     4621 actatccaaa ttatgactga gcaggagtca ggatactgct ggagaaactt tgtgaattat
     4681 agcccgagta atgaagccca ctggcctagg tatccccatc tgtgggtacg actgtacgtt
     4741 cttgaactgt actgcatcat actgggcctg cctccttgtc tcaacattct gagaaggaag
     4801 cagccacagc tgacattctt taccatcgct cttcagtctt gtcattacca gcgactgccc
     4861 ccacacattc tctgggccac cgggttgaaa ggcggtagcg gcggtagcgg tggtagcggc
     4921 ggcagcggtg gtagcggtgg cagcggtggt cgcaaacgcc cgctgatcga aaccaacggc
     4981 gaaaccggtg aaatcgtgtg ggataagggt cgcgatttcg cgaccgtgcg caaggtgctg
     5041 agcatgccgc aggtgaacat cgtgaagaag accgaagtgc agaccggcgg ttttagcaag
     5101 gaaagcatcc tgccgaagcg caacagcgat aagctgatcg cgcgtaagaa ggactgggac
     5161 ccgaaaaagt atggtggctt cgacagcccg accgtggcgt acagcgtgct ggtggtggcc
     5221 aaggtggaaa agggcaaaag caaaaaactg aaaagcgtga aggagctgct gggtattacc
     5281 attatggaac gtagcagctt tgagaagaac ccgatcgact tcctggaggc gaagggttac
     5341 aaggaagtga agaaagatct gatcatcaaa ctgccgaagt acagcctgtt tgagctggaa
     5401 aacggccgta agcgtatgct ggcgagcgcg ggtgagctgc aaaaaggtaa cgagctggcg
     5461 ctgccgagca agtacgtgaa ctttctgtac ctggcgagcc actatgaaaa actgaaaggc
     5521 agcccggagg acaacgagca gaagcagctg tttgtggagc agcacaagca ctatctggac
     5581 gaaatcatcg aacagatcag cgagttcagc aaacgcgtga ttctggccga cgccaacctg
     5641 gacaaagtgc tgagcgccta caacaagcac cgcgataaac cgattcgcga acaggccgag
     5701 aacatcattc acctgtttac cctgaccaac ctgggcgcgc cggcggcgtt caaatacttt
     5761 gataccacca ttgatcgcaa acgctacacc agcaccaagg aagtgctgga tgcgaccctg
     5821 atccaccaga gcattaccgg tctgtacgaa acccgcatcg atctgagcca gctgggcggc
     5881 gatagcggtg gtagcaagcg tccggcggcg accaaaaagg ccggccaggc gaagaagaag
     5941 aaggactaca aggatgacga tgacaaaggc agcggcgcca ccaacttcag cctgctgaag
     6001 caggccggtg acgtggaaga aaaccccggc ccgatggcga agccgctgag ccaggaggaa
     6061 agcaccctga ttgagcgcgc caccgcgacc atcaacagca ttccgattag cgaagactac
     6121 agcgtggcga gcgccgctct gagcagcgat ggccgtatct tcaccggcgt gaacgtgtac
     6181 cactttaccg gcggtccgtg cgcggaactg gtggtgctgg gcaccgcggc ggctgcggct
     6241 gcgggtaacc tgacctgcat cgtggccatt ggtaacgaaa accgcggcat tctgagcccg
     6301 tgcggccgtt gccgccaggt gctgctggac ctgcaccccg gtatcaaagc gattgtgaaa
     6361 gatagcgatg gtcaaccgac cgctgtgggt atccgtgaac tgctgccatc cggctatgtt
     6421 tgggagggct aaggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat
     6481 atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     6541 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6601 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6661 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6721 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6781 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6841 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6901 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6961 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7021 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7081 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7141 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7201 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     7261 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7321 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7381 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     7441 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7501 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     7561 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     7621 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     7681 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     7741 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7801 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     7861 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7921 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7981 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     8041 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     8101 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     8161 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     8221 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     8281 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     8341 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     8401 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     8461 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     8521 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8581 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     8641 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8701 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     8761 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8821 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8881 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8941 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9001 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9061 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9601 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10921 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cc
//
