LOCUS       sequence-267701-       11086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSpyCas9 encoding truncated human cytosine deaminase 
            hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) 
            at the C terminus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 AIDx with UGI
FEATURES             Location/Qualifiers
     source          1..11086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        519..722
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     673..693
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     767..786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        767..785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             833..1084
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5273..5293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5309..5329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5951..5998
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5999..6022
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6032..6088
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6581..6598)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6587..6811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6857..7285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6944..6963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7154..7175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7294..7314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7299..7628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7479..7614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7541..7560
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7695..8489
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7749..7768)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8359..8378
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8663..8784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8700..8719)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8754..8773
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8833..8849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8833..8849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8846..8868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8857..8873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8881..8887,8888..8905,8906..8911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8926..8947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9064..9081)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9235..9823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9315..9334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9994..10785,10786..10854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10617..10636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10855..10959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11034..11053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
       61 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      121 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      181 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      241 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      301 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      361 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      421 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      481 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      541 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      601 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      661 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      721 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      781 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgcca ccatgaccaa
      841 cctgagcgac atcattgaga aagagaccgg caagcagctg gtaatccagg aaagcattct
      901 gatgctgccg gaagaagtgg aggaagtgat tggtaacaaa ccggaaagcg acatcctggt
      961 gcacaccgct tatgacgaga gcaccgacga aaacgtgatg ctgctgacca gcgacgcgcc
     1021 ggagtataag ccgtgggcgc tggtaattca ggatagcaac ggcgaaaaca aaatcaagat
     1081 gctgggcggc gatagcggtg gtagcatagg caaggcaacc gctaagtatt ttttttacag
     1141 caacatcatg aacttcttta agaccgagat taccctggcc aacggtgaaa tccgtaaacg
     1201 cccgctgatt gaaaccaacg gtgaaaccgg cgaaatcgtg tgggacaagg gccgtgattt
     1261 tgcgaccgtg cgcaaagtgc tgtccatgcc gcaggtgaac attgtgaaga aaaccgaggt
     1321 gcagaccggc ggtttcagca aggaaagcat tctgccgaaa cgtaacagcg acaagctgat
     1381 cgcccgcaag aaagattggg acccgaagaa atacggcggt ttcgatagcc cgaccgtggc
     1441 ttatagcgtg ctggtggtgg ccaaagtgga gaagggtaaa agcaagaaac tgaagagcgt
     1501 gaaagaactg ctgggcatca ccattatgga gcgtagcagc ttcgaaaaga acccgatcga
     1561 ctttctggag gcgaagggtt acaaggaagt gaagaaagat ctgatcatta agctgccgaa
     1621 atacagcctg tttgagctgg aaaatggtcg taaacgtatg ctggccagcg ccggcgagct
     1681 gcaaaagggc aacgaactgg cgctgccgag caaatatgtg aacttcctgt acctggccag
     1741 ccactatgag aagctgaaag gtagcccgga ggacaacgaa cagaagcagc tgtttgtgga
     1801 gcagcacaaa cactatctgg atgagatcat tgaacagatc agcgagttca gcaagcgtgt
     1861 gattctggcg gacgctaacc tggataaagt gctgagcgcc tacaacaaac accgtgacaa
     1921 gccgatccgc gagcaggcgg aaaacatcat tcacctgttc accctgacca acctgggtgc
     1981 gccggccgcg ttcaagtact ttgacaccac cattgatcgt aaacgctata ccagcaccaa
     2041 ggaggtgctg gatgctaccc tgatccacca gagcattacc ggcctgtacg aaacccgtat
     2101 tgatctgagc cagctgggcg gtgacggcgg tagcggcggt agcggcggta gcggcggtag
     2161 cggcggtagc ggcggtagcg gcggtgacaa gaaatatagc atcggtctgg cgattggcac
     2221 caacagcgtg ggttgggctg tgatcaccga tgagtacaag gtgccgagca agaagttcaa
     2281 ggtgctgggc aacaccgacc gccacagcat caagaaaaac ctgattggtg ccctgctgtt
     2341 tgatagcggt gaaaccgccg aggccacccg tctgaagcgc accgcccgtc gccgttacac
     2401 ccgtcgtaaa aaccgtatct gctacctgca agagattttc agcaacgaaa tggccaaagt
     2461 ggacgatagc ttctttcacc gcctggagga gagcttcctg gtggaggaag acaagaaaca
     2521 cgagcgtcac ccgatctttg gcaacattgt ggatgaggtg gcttaccacg aaaagtatcc
     2581 gaccatctac cacctgcgta agaaactggt ggatagcacc gacaaggccg atctgcgcct
     2641 gatctatctg gctctggccc acatgatcaa gttccgtggt cactttctga tcgagggcga
     2701 cctgaacccg gataacagcg atgtggacaa gctgttcatc cagctggtgc agacctacaa
     2761 ccagctgttt gaggaaaacc cgattaacgc cagcggtgtg gacgcgaagg ctatcctgag
     2821 cgcgcgtctg agcaaaagcc gccgtctgga gaacctgatc gcgcagctgc cgggtgaaaa
     2881 gaaaaacggt ctgtttggca acctgattgc tctgagcctg ggcctgaccc cgaacttcaa
     2941 aagcaacttt gacctggcgg aggatgctaa gctgcaactg agcaaggata cctatgacga
     3001 tgacctggac aacctgctgg cgcagatcgg cgaccagtac gctgatctgt tcctggctgc
     3061 caagaacctg agcgatgcga tcctgctgag cgacattctg cgtgtgaaca ccgagattac
     3121 caaggccccg ctgagcgcga gcatgatcaa acgctatgat gaacaccacc aggacctgac
     3181 cctgctgaaa gctctggtgc gtcagcagct gccggagaag tacaaagaaa tcttctttga
     3241 ccagagcaag aacggttacg ccggctatat tgatggcggt gcgagccagg aggagttcta
     3301 caagtttatc aaaccgattc tggagaaaat ggacggcacc gaggaactgc tggtgaagct
     3361 gaaccgtgaa gatctgctgc gtaaacagcg caccttcgac aacggtagca tcccgcacca
     3421 gattcacctg ggtgagctgc acgccatcct gcgccgtcag gaagatttct acccgtttct
     3481 gaaggacaac cgtgagaaga tcgaaaaaat tctgaccttc cgcattccgt actatgtggg
     3541 tccgctggct cgtggcaaca gccgctttgc ctggatgacc cgcaagagcg aggaaaccat
     3601 caccccgtgg aacttcgagg aagtggtgga caaaggcgcc agcgcgcaga gcttcattga
     3661 gcgtatgacc aactttgata agaacctgcc gaacgaaaaa gtgctgccga agcacagcct
     3721 gctgtacgag tatttcaccg tgtacaacga actgaccaag gtgaaatatg tgaccgaggg
     3781 tatgcgcaag ccggccttcc tgagcggcga acagaagaaa gcgatcgtgg acctgctgtt
     3841 taaaaccaac cgtaaggtga ccgtgaagca gctgaaagag gattatttca agaaaattga
     3901 atgctttgac agcgtggaga tcagcggtgt ggaagatcgt tttaacgcga gcctgggcac
     3961 ctaccacgac ctgctgaaaa tcattaagga taaagacttc ctggataacg aggaaaacga
     4021 ggacatcctg gaagatattg tgctgaccct gaccctgttt gaggaccgtg aaatgatcga
     4081 ggaacgcctg aagacctatg cccacctgtt cgatgacaaa gtgatgaaac agctgaagcg
     4141 ccgtcgctac accggttggg gccgtctgag ccgcaaactg atcaacggta ttcgcgataa
     4201 acagagcggc aagaccatcc tggattttct gaagagcgac ggtttcgcga accgtaactt
     4261 tatgcagctg attcacgatg acagcctgac cttcaaagag gacatccaga aggcgcaggt
     4321 gagcggtcag ggcgatagcc tgcacgaaca tattgctaac ctggccggta gcccggccat
     4381 caagaagggc atcctgcaaa ccgtgaaggt ggtggacgag ctggtgaaag tgatgggtcg
     4441 tcacaagccg gaaaacatcg tgattgagat ggcccgcgaa aaccagacca cccagaaagg
     4501 ccagaagaac agccgtgagc gcatgaagcg tatcgaggaa ggcatcaagg aactgggcag
     4561 ccagatcctg aaggagcacc cggtggaaaa cacccagctg caaaacgaga aactgtacct
     4621 gtactacctg caaaacggtc gtgacatgta tgtggatcag gaactggaca ttaaccgcct
     4681 gagcgattat gacgtggatg cgatcgtgcc gcagagcttc ctgaaggatg acagcattga
     4741 caacaaagtg ctgacccgta gcgataaaaa ccgcggcaag agcgacaacg tgccgagcga
     4801 ggaagtggtg aagaaaatga aaaactactg gcgtcagctg ctgaacgcca aactgatcac
     4861 ccagcgtaag tttgacaacc tgaccaaagc tgagcgcggc ggtctgagcg aactggataa
     4921 agccggtttc attaagcgtc agctggtgga aacccgccag atcaccaagc acgtggcgca
     4981 gattctggac agccgcatga acaccaagta tgacgagaac gataaactga tccgtgaagt
     5041 gaaagtgatc accctgaaga gcaaactggt gagcgatttt cgtaaagact tccagtttta
     5101 caaggtgcgc gagatcaaca actaccacca cgctcacgac gcctatctga acgcggtggt
     5161 gggcaccgcc ctgatcaaga agtacccgaa gctggagagc gagttcgtgt acggcgacta
     5221 caaggtgtat gatgtgcgta agatgatcgc caaaagcgag caggaaattg gcccgaagaa
     5281 aaagcgtaaa gtgggtagca gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc
     5341 tctggatgac ttcgacctgg atatgctggg cagcgacgcc ctggatgact ttggcggtgg
     5401 cagcatggat agcctgctga tgaaccgtcg caaattcctg tatcagttta agaacgtgcg
     5461 ttgggctaaa ggccgtcgcg aaacctacct gtgctatgtg gtgaaacgtc gcgatagcgc
     5521 caccagcttc agcctggact ttggttacct gcgcaacaag aacggctgcc acgtggagct
     5581 gctgttcctg cgttacatta gcgactggga cctggaccca ggccgttgct atcgcgtgac
     5641 ctggtttacc agctggagcc cgtgctacga ttgcgctcgt catgtggcgg acttcctgcg
     5701 tggtaaccca aacctgagcc tgcgtatctt caccgcgcgc ctgtactttt gcgaggaccg
     5761 taaggctgag ccggaaggtc tgcgtcgcct gcaccgtgcg ggtgtgcaga tcgcgatcat
     5821 gaccttcaaa gattactttt attgctggaa caccttcgtg gagaaccacg aacgcacctt
     5881 taaggcctgg gagggtctgc acgaaaacag cgtgcgtctg agccgccagc tgcgccgcat
     5941 cctgctgcca aagcgtccgg cggctaccaa gaaagcgggc caggctaaga aaaagaaaga
     6001 ctacaaagat gacgatgaca agggtagcgg cgctaccaac ttcagcctgc tgaagcaggc
     6061 gggcgacgtg gaggaaaatc cgggcccgat ggctaaaccg ctgagccagg aggaaagcac
     6121 cctgatcgag cgcgctaccg ccaccattaa cagcatcccg attagcgaag actatagcgt
     6181 ggccagcgcc gcgctgagca gcgatggtcg tattttcacc ggcgtgaacg tgtaccactt
     6241 taccggtggc ccgtgcgccg agctggtggt gctgggcacc gccgccgccg ccgccgccgg
     6301 caacctgacc tgcatcgtgg cgattggtaa cgaaaatcgt ggcatcctga gcccgtgcgg
     6361 ccgttgccgc caggtgctgc tggacctgca tccgggcatc aaggcgattg tgaaggatag
     6421 cgacggccaa ccgaccgccg tgggtattcg tgaactgctg ccatccggct atgtttggga
     6481 gggctaagga tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg
     6541 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     6601 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     6661 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     6721 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     6781 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     6841 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6901 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6961 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     7021 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     7081 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     7141 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     7201 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     7261 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     7321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7381 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7561 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7621 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     7681 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     7741 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     7801 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     7861 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     7921 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     7981 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8041 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8101 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8161 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8221 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     8281 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8341 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     8401 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     8461 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     8521 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     8581 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8641 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8701 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8761 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8821 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     8881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     8941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     9061 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     9121 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     9181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     9241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     9301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     9361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     9421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     9481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     9541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     9601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     9661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     9721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     9781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     9841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     9901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     9961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    10021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    10081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    10141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    10201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    10261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    10321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    10381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    10441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    10501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    10561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    10621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    10681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    10741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    10801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    10861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    10921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    10981 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
    11041 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtg
//
