LOCUS       sequence-267707-       11350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding ABEMax (wildtype Tada monomer coupled with evolved 
            Tada monomer) at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11350)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP ABEMax strategy 1
FEATURES             Location/Qualifiers
     source          1..11350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        167..546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     701..721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     795..814
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        795..813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1956..1976
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1992..2012
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6276..6323
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6324..6347
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6357..6413
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6873..6890)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6879..7103
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7149..7577
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7236..7255)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7446..7467
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7586..7606)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7591..7920
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7771..7906
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7833..7852
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7987..8781
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8041..8060)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8651..8670
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8955..9076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8992..9011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9046..9065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9125..9141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9125..9141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9138..9160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9149..9165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9173..9179,9180..9197,9198..9203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9218..9239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9356..9373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9527..10115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9607..9626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10286..11077,11078..11146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10909..10928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11147..11251)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11326..11345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
       61 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
      121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      541 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      661 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      721 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
      781 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
      841 ttaagcttgg taccgccacc atgtctgaag tcgagtttag ccacgagtat tggatgaggc
      901 acgcactgac cctggcaaag cgagcatggg atgaaagaga agtccccgtg ggcgccgtgc
      961 tggtgcacaa caatagagtg atcggagagg gatggaacag gccaatcggc cgccacgacc
     1021 ctaccgcaca cgcagagatc atggcactga ggcagggagg cctggtcatg cagaattacc
     1081 gcctgatcga tgccaccctg tatgtgacac tggagccatg cgtgatgtgc gcaggagcaa
     1141 tgatccacag caggatcgga agagtggtgt tcggagcacg ggacgccaag accggcgcag
     1201 caggctccct gatggatgtg ctgcaccacc ccggcatgaa ccaccgggtg gagatcacag
     1261 agggaatcct ggcagacgag tgcgccgccc tgctgagcga tttctttaga atgcggagac
     1321 aggagatcaa ggcccagaag aaggcacaga gctccaccga ctctggagga tctagcggag
     1381 gatcctctgg aagcgagaca ccaggcacaa gcgagtccgc cacaccagag agctccggcg
     1441 gctcctccgg aggatcctct gaggtggagt tttcccacga gtactggatg agacatgccc
     1501 tgaccctggc caagagggca cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc
     1561 tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag
     1621 cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga
     1681 ttgacgccac cctgtacgtg acattcgagc cttgcgtgat gtgcgccggc gccatgatcc
     1741 actctaggat cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct
     1801 ccctgatgga cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa
     1861 tcctggcaga tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt
     1921 tcaatgctca gaagaaggcc cagagctcca ccgacccgaa gaaaaagcgt aaagtgggta
     1981 gcagcggcag cccgaaaaag aaacgcaaag tgggtagcga cgctctggat gacttcgacc
     2041 tggatatgct gggcagcgac gccctggatg actttggcgg tggcagcata ggcaaggcaa
     2101 ccgctaagta ttttttttac agcaacatca tgaacttctt taagaccgag attaccctgg
     2161 ccaacggtga aatccgtaaa cgcccgctga ttgaaaccaa cggtgaaacc ggcgaaatcg
     2221 tgtgggacaa gggccgtgat tttgcgaccg tgcgcaaagt gctgtccatg ccgcaggtga
     2281 acattgtgaa gaaaaccgag gtgcagaccg gcggtttcag caaggaaagc attctgccga
     2341 aacgtaacag cgacaagctg atcgcccgca agaaagattg ggacccgaag aaatacggcg
     2401 gtttcgatag cccgaccgtg gcttatagcg tgctggtggt ggccaaagtg gagaagggta
     2461 aaagcaagaa actgaagagc gtgaaagaac tgctgggcat caccattatg gagcgtagca
     2521 gcttcgaaaa gaacccgatc gactttctgg aggcgaaggg ttacaaggaa gtgaagaaag
     2581 atctgatcat taagctgccg aaatacagcc tgtttgagct ggaaaatggt cgtaaacgta
     2641 tgctggccag cgccggcgag ctgcaaaagg gcaacgaact ggcgctgccg agcaaatatg
     2701 tgaacttcct gtacctggcc agccactatg agaagctgaa aggtagcccg gaggacaacg
     2761 aacagaagca gctgtttgtg gagcagcaca aacactatct ggatgagatc attgaacaga
     2821 tcagcgagtt cagcaagcgt gtgattctgg cggacgctaa cctggataaa gtgctgagcg
     2881 cctacaacaa acaccgtgac aagccgatcc gcgagcaggc ggaaaacatc attcacctgt
     2941 tcaccctgac caacctgggt gcgccggccg cgttcaagta ctttgacacc accattgatc
     3001 gtaaacgcta taccagcacc aaggaggtgc tggatgctac cctgatccac cagagcatta
     3061 ccggcctgta cgaaacccgt attgatctga gccagctggg cggtgacggc ggtagcggcg
     3121 gtagcggcgg tagcggcggt agcggcggta gcggcggtag cggcggtgac aagaaatata
     3181 gcatcggtct ggcgattggc accaacagcg tgggttgggc tgtgatcacc gatgagtaca
     3241 aggtgccgag caagaagttc aaggtgctgg gcaacaccga ccgccacagc atcaagaaaa
     3301 acctgattgg tgccctgctg tttgatagcg gtgaaaccgc cgaggccacc cgtctgaagc
     3361 gcaccgcccg tcgccgttac acccgtcgta aaaaccgtat ctgctacctg caagagattt
     3421 tcagcaacga aatggccaaa gtggacgata gcttctttca ccgcctggag gagagcttcc
     3481 tggtggagga agacaagaaa cacgagcgtc acccgatctt tggcaacatt gtggatgagg
     3541 tggcttacca cgaaaagtat ccgaccatct accacctgcg taagaaactg gtggatagca
     3601 ccgacaaggc cgatctgcgc ctgatctatc tggctctggc ccacatgatc aagttccgtg
     3661 gtcactttct gatcgagggc gacctgaacc cggataacag cgatgtggac aagctgttca
     3721 tccagctggt gcagacctac aaccagctgt ttgaggaaaa cccgattaac gccagcggtg
     3781 tggacgcgaa ggctatcctg agcgcgcgtc tgagcaaaag ccgccgtctg gagaacctga
     3841 tcgcgcagct gccgggtgaa aagaaaaacg gtctgtttgg caacctgatt gctctgagcc
     3901 tgggcctgac cccgaacttc aaaagcaact ttgacctggc ggaggatgct aagctgcaac
     3961 tgagcaagga tacctatgac gatgacctgg acaacctgct ggcgcagatc ggcgaccagt
     4021 acgctgatct gttcctggct gccaagaacc tgagcgatgc gatcctgctg agcgacattc
     4081 tgcgtgtgaa caccgagatt accaaggccc cgctgagcgc gagcatgatc aaacgctatg
     4141 atgaacacca ccaggacctg accctgctga aagctctggt gcgtcagcag ctgccggaga
     4201 agtacaaaga aatcttcttt gaccagagca agaacggtta cgccggctat attgatggcg
     4261 gtgcgagcca ggaggagttc tacaagttta tcaaaccgat tctggagaaa atggacggca
     4321 ccgaggaact gctggtgaag ctgaaccgtg aagatctgct gcgtaaacag cgcaccttcg
     4381 acaacggtag catcccgcac cagattcacc tgggtgagct gcacgccatc ctgcgccgtc
     4441 aggaagattt ctacccgttt ctgaaggaca accgtgagaa gatcgaaaaa attctgacct
     4501 tccgcattcc gtactatgtg ggtccgctgg ctcgtggcaa cagccgcttt gcctggatga
     4561 cccgcaagag cgaggaaacc atcaccccgt ggaacttcga ggaagtggtg gacaaaggcg
     4621 ccagcgcgca gagcttcatt gagcgtatga ccaactttga taagaacctg ccgaacgaaa
     4681 aagtgctgcc gaagcacagc ctgctgtacg agtatttcac cgtgtacaac gaactgacca
     4741 aggtgaaata tgtgaccgag ggtatgcgca agccggcctt cctgagcggc gaacagaaga
     4801 aagcgatcgt ggacctgctg tttaaaacca accgtaaggt gaccgtgaag cagctgaaag
     4861 aggattattt caagaaaatt gaatgctttg acagcgtgga gatcagcggt gtggaagatc
     4921 gttttaacgc gagcctgggc acctaccacg acctgctgaa aatcattaag gataaagact
     4981 tcctggataa cgaggaaaac gaggacatcc tggaagatat tgtgctgacc ctgaccctgt
     5041 ttgaggaccg tgaaatgatc gaggaacgcc tgaagaccta tgcccacctg ttcgatgaca
     5101 aagtgatgaa acagctgaag cgccgtcgct acaccggttg gggccgtctg agccgcaaac
     5161 tgatcaacgg tattcgcgat aaacagagcg gcaagaccat cctggatttt ctgaagagcg
     5221 acggtttcgc gaaccgtaac tttatgcagc tgattcacga tgacagcctg accttcaaag
     5281 aggacatcca gaaggcgcag gtgagcggtc agggcgatag cctgcacgaa catattgcta
     5341 acctggccgg tagcccggcc atcaagaagg gcatcctgca aaccgtgaag gtggtggacg
     5401 agctggtgaa agtgatgggt cgtcacaagc cggaaaacat cgtgattgag atggcccgcg
     5461 aaaaccagac cacccagaaa ggccagaaga acagccgtga gcgcatgaag cgtatcgagg
     5521 aaggcatcaa ggaactgggc agccagatcc tgaaggagca cccggtggaa aacacccagc
     5581 tgcaaaacga gaaactgtac ctgtactacc tgcaaaacgg tcgtgacatg tatgtggatc
     5641 aggaactgga cattaaccgc ctgagcgatt atgacgtgga tgcgatcgtg ccgcagagct
     5701 tcctgaagga tgacagcatt gacaacaaag tgctgacccg tagcgataaa aaccgcggca
     5761 agagcgacaa cgtgccgagc gaggaagtgg tgaagaaaat gaaaaactac tggcgtcagc
     5821 tgctgaacgc caaactgatc acccagcgta agtttgacaa cctgaccaaa gctgagcgcg
     5881 gcggtctgag cgaactggat aaagccggtt tcattaagcg tcagctggtg gaaacccgcc
     5941 agatcaccaa gcacgtggcg cagattctgg acagccgcat gaacaccaag tatgacgaga
     6001 acgataaact gatccgtgaa gtgaaagtga tcaccctgaa gagcaaactg gtgagcgatt
     6061 ttcgtaaaga cttccagttt tacaaggtgc gcgagatcaa caactaccac cacgctcacg
     6121 acgcctatct gaacgcggtg gtgggcaccg ccctgatcaa gaagtacccg aagctggaga
     6181 gcgagttcgt gtacggcgac tacaaggtgt atgatgtgcg taagatgatc gccaaaagcg
     6241 agcaggaaat tggcggcggc gatagcggtg gtagcaagcg tccggcggct accaagaaag
     6301 cgggccaggc taagaaaaag aaagactaca aagatgacga tgacaagggt agcggcgcta
     6361 ccaacttcag cctgctgaag caggcgggcg acgtggagga aaatccgggc ccgatggcta
     6421 aaccgctgag ccaggaggaa agcaccctga tcgagcgcgc taccgccacc attaacagca
     6481 tcccgattag cgaagactat agcgtggcca gcgccgcgct gagcagcgat ggtcgtattt
     6541 tcaccggcgt gaacgtgtac cactttaccg gtggcccgtg cgccgagctg gtggtgctgg
     6601 gcaccgccgc cgccgccgcc gccggcaacc tgacctgcat cgtggcgatt ggtaacgaaa
     6661 atcgtggcat cctgagcccg tgcggccgtt gccgccaggt gctgctggac ctgcatccgg
     6721 gcatcaaggc gattgtgaag gatagcgacg gccaaccgac cgccgtgggt attcgtgaac
     6781 tgctgccatc cggctatgtt tgggagggct aagatatcca gcacagtggc ggccgctcga
     6841 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6901 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6961 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     7021 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     7081 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     7141 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7201 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7261 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7321 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7381 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7441 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7501 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7561 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7681 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7741 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7801 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7861 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7921 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     7981 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     8041 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     8101 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     8161 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     8221 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     8281 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     8341 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     8401 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     8461 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     8521 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     8581 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     8641 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     8701 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     8761 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     8821 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8881 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8941 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9001 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9061 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9121 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9181 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9241 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     9301 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     9361 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     9421 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     9481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     9541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     9601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     9661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9901 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9961 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10021 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10081 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10141 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10201 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10261 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    10321 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    10381 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10441 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10501 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10561 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10621 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10681 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10741 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10801 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10861 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10921 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10981 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11041 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11101 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11161 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11221 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11281 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    11341 tgccgcatag
//
