LOCUS       sequence-267714-       11305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding de-coupled ABEMax (wildtype Tada monomer at N 
            terminus, evolved Tada monomer at C terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP ABEMax strategy 2
FEATURES             Location/Qualifiers
     source          1..11305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        538..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     692..712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     786..805
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        786..804
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1353..1373
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1389..1409
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5652..5699
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6303..6359
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6819..6836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6825..7049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7095..7523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7182..7201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7392..7413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7532..7552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7537..7866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7717..7852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7779..7798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7933..8727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7987..8006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8597..8616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8901..9022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8938..8957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8992..9011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9071..9087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9071..9087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9084..9106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9095..9111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9119..9125,9126..9143,9144..9149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9164..9185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9302..9319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9473..10061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9553..9572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10232..11023,11024..11092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10855..10874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11093..11197)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11272..11291)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
       61 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      121 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      541 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      661 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      721 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
      781 gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg
      841 gtaccgccac catgtctgag gtggagtttt cccacgagta ctggatgaga catgccctga
      901 ccctggccaa gagggcacgc gatgagaggg aggtgcctgt gggagccgtg ctggtgctga
      961 acaatagagt gatcggcgag ggctggaaca gagccatcgg cctgcacgac ccaacagccc
     1021 atgccgaaat tatggccctg agacagggcg gcctggtcat gcagaactac agactgattg
     1081 acgccaccct gtacgtgaca ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact
     1141 ctaggatcgg ccgcgtggtg tttggcgtga ggaacgcaaa aaccggcgcc gcaggctccc
     1201 tgatggacgt gctgcactac cccggcatga atcaccgcgt cgaaattacc gagggaatcc
     1261 tggcagatga atgtgccgcc ctgctgtgct atttctttcg gatgcctaga caggtgttca
     1321 atgctcagaa gaaggcccag agctccaccg acccgaagaa aaagcgtaaa gtgggtagca
     1381 gcggcagccc gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac ttcgacctgg
     1441 atatgctggg cagcgacgcc ctggatgact ttggcggtgg cagcataggc aaggcaaccg
     1501 ctaagtattt tttttacagc aacatcatga acttctttaa gaccgagatt accctggcca
     1561 acggtgaaat ccgtaaacgc ccgctgattg aaaccaacgg tgaaaccggc gaaatcgtgt
     1621 gggacaaggg ccgtgatttt gcgaccgtgc gcaaagtgct gtccatgccg caggtgaaca
     1681 ttgtgaagaa aaccgaggtg cagaccggcg gtttcagcaa ggaaagcatt ctgccgaaac
     1741 gtaacagcga caagctgatc gcccgcaaga aagattggga cccgaagaaa tacggcggtt
     1801 tcgatagccc gaccgtggct tatagcgtgc tggtggtggc caaagtggag aagggtaaaa
     1861 gcaagaaact gaagagcgtg aaagaactgc tgggcatcac cattatggag cgtagcagct
     1921 tcgaaaagaa cccgatcgac tttctggagg cgaagggtta caaggaagtg aagaaagatc
     1981 tgatcattaa gctgccgaaa tacagcctgt ttgagctgga aaatggtcgt aaacgtatgc
     2041 tggccagcgc cggcgagctg caaaagggca acgaactggc gctgccgagc aaatatgtga
     2101 acttcctgta cctggccagc cactatgaga agctgaaagg tagcccggag gacaacgaac
     2161 agaagcagct gtttgtggag cagcacaaac actatctgga tgagatcatt gaacagatca
     2221 gcgagttcag caagcgtgtg attctggcgg acgctaacct ggataaagtg ctgagcgcct
     2281 acaacaaaca ccgtgacaag ccgatccgcg agcaggcgga aaacatcatt cacctgttca
     2341 ccctgaccaa cctgggtgcg ccggccgcgt tcaagtactt tgacaccacc attgatcgta
     2401 aacgctatac cagcaccaag gaggtgctgg atgctaccct gatccaccag agcattaccg
     2461 gcctgtacga aacccgtatt gatctgagcc agctgggcgg tgacggcggt agcggcggta
     2521 gcggcggtag cggcggtagc ggcggtagcg gcggtagcgg cggtgacaag aaatatagca
     2581 tcggtctggc gattggcacc aacagcgtgg gttgggctgt gatcaccgat gagtacaagg
     2641 tgccgagcaa gaagttcaag gtgctgggca acaccgaccg ccacagcatc aagaaaaacc
     2701 tgattggtgc cctgctgttt gatagcggtg aaaccgccga ggccacccgt ctgaagcgca
     2761 ccgcccgtcg ccgttacacc cgtcgtaaaa accgtatctg ctacctgcaa gagattttca
     2821 gcaacgaaat ggccaaagtg gacgatagct tctttcaccg cctggaggag agcttcctgg
     2881 tggaggaaga caagaaacac gagcgtcacc cgatctttgg caacattgtg gatgaggtgg
     2941 cttaccacga aaagtatccg accatctacc acctgcgtaa gaaactggtg gatagcaccg
     3001 acaaggccga tctgcgcctg atctatctgg ctctggccca catgatcaag ttccgtggtc
     3061 actttctgat cgagggcgac ctgaacccgg ataacagcga tgtggacaag ctgttcatcc
     3121 agctggtgca gacctacaac cagctgtttg aggaaaaccc gattaacgcc agcggtgtgg
     3181 acgcgaaggc tatcctgagc gcgcgtctga gcaaaagccg ccgtctggag aacctgatcg
     3241 cgcagctgcc gggtgaaaag aaaaacggtc tgtttggcaa cctgattgct ctgagcctgg
     3301 gcctgacccc gaacttcaaa agcaactttg acctggcgga ggatgctaag ctgcaactga
     3361 gcaaggatac ctatgacgat gacctggaca acctgctggc gcagatcggc gaccagtacg
     3421 ctgatctgtt cctggctgcc aagaacctga gcgatgcgat cctgctgagc gacattctgc
     3481 gtgtgaacac cgagattacc aaggccccgc tgagcgcgag catgatcaaa cgctatgatg
     3541 aacaccacca ggacctgacc ctgctgaaag ctctggtgcg tcagcagctg ccggagaagt
     3601 acaaagaaat cttctttgac cagagcaaga acggttacgc cggctatatt gatggcggtg
     3661 cgagccagga ggagttctac aagtttatca aaccgattct ggagaaaatg gacggcaccg
     3721 aggaactgct ggtgaagctg aaccgtgaag atctgctgcg taaacagcgc accttcgaca
     3781 acggtagcat cccgcaccag attcacctgg gtgagctgca cgccatcctg cgccgtcagg
     3841 aagatttcta cccgtttctg aaggacaacc gtgagaagat cgaaaaaatt ctgaccttcc
     3901 gcattccgta ctatgtgggt ccgctggctc gtggcaacag ccgctttgcc tggatgaccc
     3961 gcaagagcga ggaaaccatc accccgtgga acttcgagga agtggtggac aaaggcgcca
     4021 gcgcgcagag cttcattgag cgtatgacca actttgataa gaacctgccg aacgaaaaag
     4081 tgctgccgaa gcacagcctg ctgtacgagt atttcaccgt gtacaacgaa ctgaccaagg
     4141 tgaaatatgt gaccgagggt atgcgcaagc cggccttcct gagcggcgaa cagaagaaag
     4201 cgatcgtgga cctgctgttt aaaaccaacc gtaaggtgac cgtgaagcag ctgaaagagg
     4261 attatttcaa gaaaattgaa tgctttgaca gcgtggagat cagcggtgtg gaagatcgtt
     4321 ttaacgcgag cctgggcacc taccacgacc tgctgaaaat cattaaggat aaagacttcc
     4381 tggataacga ggaaaacgag gacatcctgg aagatattgt gctgaccctg accctgtttg
     4441 aggaccgtga aatgatcgag gaacgcctga agacctatgc ccacctgttc gatgacaaag
     4501 tgatgaaaca gctgaagcgc cgtcgctaca ccggttgggg ccgtctgagc cgcaaactga
     4561 tcaacggtat tcgcgataaa cagagcggca agaccatcct ggattttctg aagagcgacg
     4621 gtttcgcgaa ccgtaacttt atgcagctga ttcacgatga cagcctgacc ttcaaagagg
     4681 acatccagaa ggcgcaggtg agcggtcagg gcgatagcct gcacgaacat attgctaacc
     4741 tggccggtag cccggccatc aagaagggca tcctgcaaac cgtgaaggtg gtggacgagc
     4801 tggtgaaagt gatgggtcgt cacaagccgg aaaacatcgt gattgagatg gcccgcgaaa
     4861 accagaccac ccagaaaggc cagaagaaca gccgtgagcg catgaagcgt atcgaggaag
     4921 gcatcaagga actgggcagc cagatcctga aggagcaccc ggtggaaaac acccagctgc
     4981 aaaacgagaa actgtacctg tactacctgc aaaacggtcg tgacatgtat gtggatcagg
     5041 aactggacat taaccgcctg agcgattatg acgtggatgc gatcgtgccg cagagcttcc
     5101 tgaaggatga cagcattgac aacaaagtgc tgacccgtag cgataaaaac cgcggcaaga
     5161 gcgacaacgt gccgagcgag gaagtggtga agaaaatgaa aaactactgg cgtcagctgc
     5221 tgaacgccaa actgatcacc cagcgtaagt ttgacaacct gaccaaagct gagcgcggcg
     5281 gtctgagcga actggataaa gccggtttca ttaagcgtca gctggtggaa acccgccaga
     5341 tcaccaagca cgtggcgcag attctggaca gccgcatgaa caccaagtat gacgagaacg
     5401 ataaactgat ccgtgaagtg aaagtgatca ccctgaagag caaactggtg agcgattttc
     5461 gtaaagactt ccagttttac aaggtgcgcg agatcaacaa ctaccaccac gctcacgacg
     5521 cctatctgaa cgcggtggtg ggcaccgccc tgatcaagaa gtacccgaag ctggagagcg
     5581 agttcgtgta cggcgactac aaggtgtatg atgtgcgtaa gatgatcgcc aaaagcgagc
     5641 aggaaattgg caagcgtccg gcggctacca agaaagcggg ccaggctaag aaaaagaaat
     5701 ctggaggatc tagcggagga tcctctggaa gcgagacacc aggcacaagc gagtccgcca
     5761 caccagagag ctccggcggc tcctccggag gatcctctga agtcgagttt agccacgagt
     5821 attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga gaagtccccg
     5881 tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac aggccaatcg
     5941 gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga ggcctggtca
     6001 tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca tgcgtgatgt
     6061 gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca cgggacgcca
     6121 agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg aaccaccggg
     6181 tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc gatttcttta
     6241 gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc gacggtagcg
     6301 gcgctaccaa cttcagcctg ctgaagcagg cgggcgacgt ggaggaaaat ccgggcccga
     6361 tggctaaacc gctgagccag gaggaaagca ccctgatcga gcgcgctacc gccaccatta
     6421 acagcatccc gattagcgaa gactatagcg tggccagcgc cgcgctgagc agcgatggtc
     6481 gtattttcac cggcgtgaac gtgtaccact ttaccggtgg cccgtgcgcc gagctggtgg
     6541 tgctgggcac cgccgccgcc gccgccgccg gcaacctgac ctgcatcgtg gcgattggta
     6601 acgaaaatcg tggcatcctg agcccgtgcg gccgttgccg ccaggtgctg ctggacctgc
     6661 atccgggcat caaggcgatt gtgaaggata gcgacggcca accgaccgcc gtgggtattc
     6721 gtgaactgct gccatccggc tatgtttggg agggctaaga tatccagcac agtggcggcc
     6781 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     6841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7081 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7861 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     7921 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7981 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8041 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8101 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8161 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8221 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8281 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8341 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8401 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8461 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8521 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8581 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8641 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8701 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     8761 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8821 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8881 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9001 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9061 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10081 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10141 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10201 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10261 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10321 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10381 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10441 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10501 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10561 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10621 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10681 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10741 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10801 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10861 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10921 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10981 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11041 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11101 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11161 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11221 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
    11281 ctctgatgcc gcatagttaa gccag
//
