LOCUS       sequence-267715-       10771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSpyCas9 with N-terminal miniABEMax (V82G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: miniABEMax V82G wt
FEATURES             Location/Qualifiers
     source          1..10771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        514..717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     668..688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     762..781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        762..780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             858..878
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1473..1496,1497..1499,1500..3986,3987..3989,
                     3990..5573)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5586..5606
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5619..5666
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5667..5690
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5700..5756
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6261..6278)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6267..6491
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6537..6965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6624..6643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6834..6855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6974..6994)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6979..7308
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7159..7294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7221..7240
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7375..8169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7429..7448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8039..8058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8343..8464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8380..8399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8434..8453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8513..8529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8513..8529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8526..8548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8537..8553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8561..8567,8568..8585,8586..8591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8606..8627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8744..8761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8915..9503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8995..9014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9674..10465,10466..10534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10297..10316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10535..10639)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10714..10733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
       61 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      121 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      721 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      781 gagacccaag ctggctagcg tttaaactta agcttgccac catgaaacgg acagccgacg
      841 gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc tgaggtggag ttttcccacg
      901 agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc
      961 ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca
     1021 tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg
     1081 tcatgcagaa ctacagactg attgacgcca ccctgtacgg cacattcgag ccttgcgtga
     1141 tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaacg
     1201 caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc
     1261 gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct
     1321 ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc accgactccg
     1381 gaggatctag cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac
     1441 ctgaaagcag cgggggcagc agcggggggt cagataaaaa gtatagcatt ggtctggcga
     1501 ttggcaccaa cagcgtgggc tgggccgtga ttaccgatga gtacaaagtt ccgtccaaga
     1561 agttcaaggt gctgggtaac accgaccgcc acagcatcaa gaaaaacctg attggtgccc
     1621 tgctgtttga tagcggtgaa accgccgagg ctacccgtct gaagcgcacc gcccgtcgcc
     1681 gttatacccg tcgtaaaaac cgtatctgct acctgcaaga gattttcagc aacgaaatgg
     1741 ccaaagtgga cgatagcttc tttcaccgcc tggaggagag cttcctggtg gaggaagaca
     1801 agaaacacga gcgtcacccg atctttggca acattgtgga tgaggtggct tatcacgaaa
     1861 agtacccgac catctatcac ctgcgtaaga aactggtgga tagcaccgac aaggccgatc
     1921 tgcgcctgat ctacctggcg ctggctcaca tgatcaagtt ccgtggtcac tttctgatcg
     1981 agggcgacct gaacccggat aacagcgatg tggacaagct gttcatccag ctggtgcaga
     2041 cctataacca gctgtttgag gaaaacccga ttaacgccag cggtgtggac gccaaggcga
     2101 tcctgagcgc gcgtctgagc aaaagccgcc gtctggagaa cctgatcgcg cagctgccgg
     2161 gtgaaaagaa aaacggtctg tttggcaacc tgattgctct gagcctgggc ctgaccccga
     2221 acttcaaaag caactttgac ctggccgagg atgcgaagct gcaactgagc aaggatacct
     2281 acgacgatga cctggacaac ctgctggcgc agatcggcga ccagtatgct gatctgttcc
     2341 tggccgcgaa gaacctgagc gatgcgatcc tgctgagcga cattctgcgt gtgaacaccg
     2401 agattaccaa ggctccgctg agcgccagca tgatcaaacg ctacgatgaa caccaccagg
     2461 acctgaccct gctgaaagct ctggtgcgtc agcagctgcc ggagaagtat aaagaaatct
     2521 tctttgacca gagcaagaac ggttacgccg gctatattga tggcggtgcg agccaggagg
     2581 agttctacaa gtttatcaaa ccgattctgg agaaaatgga cggcaccgag gaactgctgg
     2641 tgaagctgaa ccgtgaagat ctgctgcgta aacagcgcac ctttgacaac ggtagcatcc
     2701 cgcaccagat tcacctgggt gagctgcacg ccatcctgcg ccgtcaggaa gatttctacc
     2761 cgtttctgaa ggacaaccgt gagaagatcg aaaaaattct gaccttccgc attccgtact
     2821 atgtgggtcc gctggctcgt ggcaacagcc gctttgcctg gatgacccgc aagagcgagg
     2881 aaaccatcac cccgtggaac ttcgaggaag tggtggacaa aggcgccagc gcccagagct
     2941 tcattgagcg tatgaccaac tttgataaga acctgccgaa cgaaaaagtg ctgccgaagc
     3001 acagcctgct gtacgagtat ttcaccgtgt ataacgaact gaccaaggtg aaatacgtga
     3061 ccgagggtat gcgcaagccg gccttcctga gcggcgaaca gaagaaagcg atcgtggacc
     3121 tgctgtttaa aaccaaccgt aaggtgaccg tgaagcagct gaaagaggat tacttcaaga
     3181 aaattgaatg cttcgacagc gtggagatca gcggtgtgga agatcgtttt aacgcgagcc
     3241 tgggcaccta tcacgacctg ctgaaaatca ttaaggataa agacttcctg gataacgagg
     3301 aaaacgagga catcctggaa gatattgtgc tgaccctgac cctgtttgag gaccgtgaaa
     3361 tgatcgagga acgcctgaag acctacgccc acctgttcga tgacaaagtg atgaaacagc
     3421 tgaagcgccg tcgctatacc ggttggggcc gtctgagccg caaactgatc aacggtattc
     3481 gcgataaaca gagcggcaag accatcctgg attttctgaa gagcgacggt ttcgcgaacc
     3541 gtaactttat gcagctgatt cacgatgaca gcctgacctt caaagaggac atccagaagg
     3601 cgcaggtgag cggtcagggc gatagcctgc acgaacacat cgctaacctg gccggtagcc
     3661 cggccatcaa gaagggcatc ctgcaaaccg tgaaggtggt ggacgagctg gtgaaagtga
     3721 tgggtcgtca caagccggaa aacatcgtga ttgagatggc ccgcgaaaac cagaccaccc
     3781 agaaaggcca gaagaacagc cgtgagcgca tgaagcgtat cgaggaaggc atcaaggaac
     3841 tgggcagcca gatcctgaag gagcacccgg tggaaaacac ccagctgcaa aacgagaaac
     3901 tgtatctgta ctacctgcaa aacggtcgtg acatgtacgt ggatcaggag ctggacatta
     3961 accgcctgag cgattacgac gtggatgcga tcgtgccgca gagcttcctg aaggatgaca
     4021 gcattgacaa caaagtgctg acccgtagcg ataaaaaccg cggcaagagc gacaacgtgc
     4081 cgagcgagga agtggtgaag aaaatgaaaa actactggcg tcagctgctg aacgccaaac
     4141 tgatcaccca gcgtaagttt gacaacctga ccaaagctga gcgcggcggt ctgagcgaac
     4201 tggataaagc cggtttcatt aagcgtcagc tggtggaaac ccgccagatc accaagcacg
     4261 tggcgcagat tctggacagc cgcatgaaca ccaagtacga cgagaacgat aaactgatcc
     4321 gtgaagtgaa agtgatcacc ctgaagagca aactggtgag cgattttcgt aaagacttcc
     4381 agttttacaa ggtgcgcgag atcaacaact atcaccacgc gcacgacgct tacctgaacg
     4441 ccgtggtggg caccgccctg atcaagaagt atccgaagct ggagagcgag ttcgtgtacg
     4501 gcgattataa agtgtacgac gtgcgtaaaa tgatcgcgaa gagcgagcag gaaattggca
     4561 aagcgaccgc taagtatttc ttttacagca acatcatgaa cttctttaag accgagatca
     4621 ccctggccaa cggtgaaatc cgtaaacgcc cgctgattga aaccaacggt gaaaccggcg
     4681 aaatcgtgtg ggataagggc cgtgactttg cgaccgtgcg caaagtgctg tccatgccgc
     4741 aggtgaacat tgtgaagaaa accgaggtgc agaccggcgg tttcagcaag gaaagcattc
     4801 tgccgaaacg taacagcgac aagctgatcg cccgcaagaa agactgggac ccgaagaagt
     4861 atggcggttt cgatagccca accgtggcct acagcgtgct ggtggtggct aaagtggaga
     4921 agggtaaaag caagaaactg aaaagcgtga aggaactgct gggcatcacc attatggagc
     4981 gtagcagctt cgaaaagaac ccgatcgact ttctggaggc gaaaggttac aaggaagtga
     5041 agaaagatct gatcatcaag ctgccgaagt atagcctgtt tgagctggaa aatggtcgta
     5101 aacgtatgct ggccagcgcc ggcgagctgc aaaagggcaa cgaactggcg ctgccgagca
     5161 aatacgtgaa cttcctgtat ctggccagcc actacgagaa gctgaaaggt agcccggagg
     5221 acaacgaaca gaagcagctg tttgtggagc agcacaaaca ctacctggat gagatcattg
     5281 aacagattag cgagttcagc aagcgtgtga tcctggctga tgccaacctg gacaaagtgc
     5341 tgagcgccta taacaaacac cgtgacaagc cgatccgcga gcaggcggaa aacatcattc
     5401 acctgttcac cctgaccaac ctgggtgcgc cggctgcctt caagtatttt gataccacca
     5461 ttgaccgtaa acgctacacc agcaccaagg aggtgctgga tgctaccctg atccaccaga
     5521 gcattaccgg cctgtacgaa acccgtatcg atctgagcca gctgggcggt gactctggtg
     5581 gttctcccaa gaagaagagg aaagtctctg gtggttctaa gcgtccggcg gctaccaaga
     5641 aagccggtca ggcgaagaaa aagaaagact acaaagatga cgatgacaag ggtagcggcg
     5701 ctaccaactt cagcctgctg aagcaggcgg gcgacgtgga ggaaaatccg ggcccgatgg
     5761 ctaaaccgct gagccaggag gaaagcaccc tgatcgagcg cgcgaccgct accattaaca
     5821 gcatcccgat tagcgaagat tatagcgtgg ccagcgccgc gctgagcagc gatggtcgta
     5881 tcttcaccgg cgtgaacgtg tatcacttta ccggtggccc gtgcgccgag ctggtggtgc
     5941 tgggcaccgc cgccgccgcc gccgccggca acctgacctg catcgtggcg attggtaacg
     6001 aaaatcgtgg catcctgagc ccgtgcggcc gttgccgcca ggtgctgctg gacctgcatc
     6061 cgggcatcaa ggcgattgtg aaggatagcg acggccaacc gaccgctgtg ggtattcgtg
     6121 aactgctgcc atccggctat gtttgggaag gctaaggtac cgagctcgga tccactagtc
     6181 cagtgtggtg gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     6241 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6301 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6361 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6421 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6481 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6961 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7021 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7081 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7141 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7201 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7261 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7321 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     7381 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7441 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7501 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7561 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7621 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7681 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7741 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7801 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7861 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     7921 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7981 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     8041 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     8101 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     8161 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     8221 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     8281 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     8341 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8461 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8521 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8581 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8701 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9541 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9601 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10681 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    10741 aagccagtat ctgctccctg cttgtgtgtt g
//
