LOCUS       sequence-267718-       10372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresion of SaCas9 nickase (D10A) with ABEMax at the N terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: ABEMax nSaCas9
FEATURES             Location/Qualifiers
     source          1..10372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     639..659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..752
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        733..751
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             813..833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5193..5213
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5226..5273
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5274..5297
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5307..5363
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5364..5762
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5833..5850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5839..6063
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6109..6537
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6196..6215)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6406..6427
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6546..6566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6551..6880
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6731..6866
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6793..6812
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6947..7741
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7001..7020)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7611..7630
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7915..8036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7952..7971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8006..8025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8085..8101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8085..8101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8098..8120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8109..8125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8133..8139,8140..8157,8158..8163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8178..8199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8316..8333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8487..9075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8567..8586)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9246..10037,10038..10106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9869..9888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10107..10211)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10286..10305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc gccaccatga
      781 aacggacagc cgacggaagc gagttcgagt caccaaagaa gaagcggaaa gtctctgaag
      841 tcgagtttag ccacgagtat tggatgaggc acgcactgac cctggcaaag cgagcatggg
      901 atgaaagaga agtccccgtg ggcgccgtgc tggtgcacaa caatagagtg atcggagagg
      961 gatggaacag gccaatcggc cgccacgacc ctaccgcaca cgcagagatc atggcactga
     1021 ggcagggagg cctggtcatg cagaattacc gcctgatcga tgccaccctg tatgtgacac
     1081 tggagccatg cgtgatgtgc gcaggagcaa tgatccacag caggatcgga agagtggtgt
     1141 tcggagcacg ggacgccaag accggcgcag caggctccct gatggatgtg ctgcaccacc
     1201 ccggcatgaa ccaccgggtg gagatcacag agggaatcct ggcagacgag tgcgccgccc
     1261 tgctgagcga tttctttaga atgcggagac aggagatcaa ggcccagaag aaggcacaga
     1321 gctccaccga ctctggagga tctagcggag gatcctctgg aagcgagaca ccaggcacaa
     1381 gcgagtccgc cacaccagag agctccggcg gctcctccgg aggatcctct gaggtggagt
     1441 tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca cgcgatgaga
     1501 gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc gagggctgga
     1561 acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc ctgagacagg
     1621 gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacgtg acattcgagc
     1681 cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg gtgtttggcg
     1741 tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac taccccggca
     1801 tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc gccctgctgt
     1861 gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc cagagctcca
     1921 ccgactccgg aggatctagc ggaggctcct ctggctctga gacacctggc acaagcgaga
     1981 gcgcaacacc tgaaagcagc gggggcagca gcggggggtc agggaagcga aattacattc
     2041 tggggctggc cattggcatt acatcagtgg gctatggcat cattgactac gagacaaggg
     2101 acgtgatcga cgccggcgtg agactgttca aggaggccaa cgtggagaac aatgagggcc
     2161 ggagatccaa gaggggagca aggcgcctga agcggagaag gcgccacaga atccagagag
     2221 tgaagaagct gctgttcgat tacaacctgc tgaccgacca ctccgagctg tctggcatca
     2281 atccttatga ggccagagtg aagggcctgt cccagaagct gtctgaggag gagtttagcg
     2341 ccgccctgct gcacctggca aagaggagag gcgtgcacaa cgtgaatgag gtggaggagg
     2401 acaccggcaa cgagctgtcc acaaaggagc agatcagccg caattccaag gccctggagg
     2461 agaagtatgt ggccgagctg cagctggagc ggctgaagaa ggatggcgag gtgaggggct
     2521 ccatcaatcg cttcaagacc tctgactacg tgaaggaggc caagcagctg ctgaaggtgc
     2581 agaaggccta ccaccagctg gatcagtcct ttatcgatac atatatcgac ctgctggaga
     2641 caaggcgcac atactatgag ggaccaggag agggctctcc cttcggctgg aaggacatca
     2701 aggagtggta cgagatgctg atgggccact gcacctattt tccagaggag ctgagaagcg
     2761 tgaagtacgc ctataacgcc gatctgtaca acgccctgaa tgacctgaac aacctggtca
     2821 tcaccaggga tgagaacgag aagctggagt actatgagaa gttccagatc atcgagaacg
     2881 tgttcaagca gaagaagaag cctacactga agcagatcgc caaggagatc ctggtgaacg
     2941 aggaggacat caagggctac cgcgtgacct ccacaggcaa gccagagttc accaatctga
     3001 aggtgtatca cgatatcaag gacatcacag cccggaagga gatcatcgag aacgccgagc
     3061 tgctggatca gatcgccaag atcctgacca tctatcagag ctccgaggac atccaggagg
     3121 agctgaccaa cctgaatagc gagctgacac aggaggagat cgagcagatc agcaatctga
     3181 agggctacac cggcacacac aacctgagcc tgaaggccat caatctgatc ctggatgagc
     3241 tgtggcacac aaacgacaat cagatcgcca tctttaaccg gctgaagctg gtgccaaaga
     3301 aggtggacct gtcccagcag aaggagatcc caaccacact ggtggacgat ttcatcctgt
     3361 ctcccgtggt gaagcggagc ttcatccaga gcatcaaagt gatcaacgcc atcatcaaga
     3421 agtacggcct gcccaatgat atcatcatcg agctggccag ggagaagaac tccaaggacg
     3481 cccagaagat gatcaatgag atgcagaaga ggaaccgcca gaccaatgag cggatcgagg
     3541 agatcatcag aaccacaggc aaggagaacg ccaagtacct gatcgagaag atcaagctgc
     3601 acgatatgca ggagggcaag tgtctgtatt ctctggaggc catccctctg gaggacctgc
     3661 tgaacaatcc attcaactac gaggtggatc acatcatccc ccggagcgtg agcttcgaca
     3721 attcttttaa caataaggtg ctggtgaagc aggaggagaa cagcaagaag ggcaatagga
     3781 cccctttcca gtacctgtct agctccgatt ctaagatcag ctacgagaca ttcaagaagc
     3841 acatcctgaa tctggccaag ggcaagggcc gcatcagcaa gaccaagaag gagtacctgc
     3901 tggaggagcg ggacatcaac agattctccg tgcagaagga cttcatcaac cggaatctgg
     3961 tggacaccag atacgccaca cgcggcctga tgaatctgct gcggtcttat ttcagagtga
     4021 acaatctgga tgtgaaggtg aagagcatca acggcggctt cacctccttt ctgcggagaa
     4081 agtggaagtt taagaaggag cgcaacaagg gctataagca ccacgccgag gatgccctga
     4141 tcatcgccaa tgccgacttc atctttaagg agtggaagaa gctggacaag gccaagaaag
     4201 tgatggagaa ccagatgttc gaggagaagc aggccgagag catgcccgag atcgagacag
     4261 agcaggagta caaggagatt ttcatcacac ctcaccagat caagcacatc aaggacttca
     4321 aggactacaa gtattctcac agggtggata agaagcccaa ccgcgagctg atcaatgaca
     4381 ccctgtatag cacacggaag gacgataagg gcaataccct gatcgtgaac aatctgaacg
     4441 gcctgtacga caaggataat gacaagctga agaagctgat caacaagtct cccgagaagc
     4501 tgctgatgta ccaccacgat cctcagacat atcagaagct gaagctgatc atggagcagt
     4561 acggcgacga gaagaaccca ctgtataagt actatgagga gacaggcaac tacctgacaa
     4621 agtatagcaa gaaggataat ggccccgtga tcaagaagat caagtactat ggcaacaagc
     4681 tgaatgccca cctggacatc accgacgatt accctaactc tcgcaataag gtggtgaagc
     4741 tgagcctgaa gccataccgg ttcgacgtgt acctggacaa cggcgtgtat aagtttgtga
     4801 cagtgaagaa tctggatgtg atcaagaagg agaactacta tgaggtgaac agcaagtgct
     4861 acgaggaggc caagaagctg aagaagatca gcaaccaggc cgagttcatc gcctcttttt
     4921 acaacaatga cctgatcaag atcaatggcg agctgtatag agtgatcggc gtgaacaatg
     4981 atctgctgaa cagaatcgaa gtgaatatga tcgacatcac ctacagggag tatctggaga
     5041 acatgaatga taagaggccc cctcgcatca tcaagaccat cgcctctaag acacagagca
     5101 tcaagaagta cagcacagac atcctgggga acctgtatga agtcaagagc aagaaacatc
     5161 ctcagattat caagaaaggc tctggtggtt ctcccaagaa gaagaggaaa gtctctggtg
     5221 gttctaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca
     5281 aagacgatga cgataaggga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     5341 atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa tccaccctca
     5401 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     5461 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     5521 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5581 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5641 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5701 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     5761 aagaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     5821 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5881 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5941 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6001 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6061 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6121 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6181 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6241 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6301 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6361 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6421 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6481 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6541 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6721 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6781 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6841 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6901 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6961 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7021 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7081 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7141 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7201 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7261 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7321 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7381 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7441 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7501 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7561 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7621 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7681 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7741 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7801 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7861 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7921 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7981 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8041 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8101 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8161 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8221 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8281 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8341 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8401 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8641 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8701 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8761 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8821 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8881 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8941 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9001 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9061 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9121 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9181 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9241 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9301 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9361 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9421 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9481 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9541 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9601 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9661 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9721 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9781 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9841 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9901 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9961 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10021 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10081 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10141 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10201 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    10261 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10321 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa at
//
