LOCUS       sequence-267730-       20247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Drosophila attB-targeting plasmid that contains UAS driving 
            nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, 
            mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp 
            recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20247)
  AUTHORS   Veling MW, Li Y, Veling MT, Litts C, Michki N, Liu H, Ye B, Cai D
  TITLE     Identification of Neuronal Lineages in the Drosophila Peripheral 
            Nervous System with a ""Digital"" Multi-spectral Lineage Tracing 
            System.
  JOURNAL   Cell Rep. 2019 Dec 3;29(10):3303-3312.e3. doi: 
            10.1016/j.celrep.2019.10.124.
  PUBMED    31801091
REFERENCE   2  (bases 1 to 20247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.10"; journalName: "Cell Rep"; date: 
            "2019-12-3- 3"; volume: "29"; issue: "10"; pages: "3303-3312.e3"
COMMENT     Sequence Label: pDC-UAS-nBitbow1.0(miniW)
FEATURES             Location/Qualifiers
     source          1..20247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6513..6607
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6653..6747
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6778..7016
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    7026..7059
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     CDS             complement(7098..7127)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(7137..7850,7851..7853,7854..7856))
                     /codon_start=1
                     /product="photostable yellow fluorescent protein with a 
                     large Stokes shift, excitable with violet light (Ai et al.,
                     2008)"
                     /label=mAmetrine1.1
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVRGEGEGDATNGKLTL
                     KFICTSGELPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNAWDAYITADKQKNGIK
                     ANFKIEHNVKDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     7790..7811
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7853..7862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7875..8252)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     protein_bind    complement(8305..8338)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    9132..9226
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    9272..9366
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        9397..9635
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    9645..9678
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(9715..9744)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(9771..10475)
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             complement(9771..10475)
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             complement(10545..11249)
                     /codon_start=1
                     /product="improved version of the oligomeric far-red 
                     fluorescent protein TurboFP635/Katushka (Luker et al., 
                     2015)"
                     /label=Katushka2S
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     CDS             complement(10545..11249)
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHAQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     regulatory      11246..11255
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(11268..11645)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     protein_bind    complement(11698..11731)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    12522..12616
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    12662..12756
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        12787..13025
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    13035..13068
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(13105..13134)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(13143..13853)
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      13589..13598
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13850..13859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(13872..14249)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     protein_bind    complement(14302..14335)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    15076..15170
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    15216..15310
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        15341..15579
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             complement(15658..15687)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(15697..16407)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     regulatory      16404..16413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(16426..16803)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     protein_bind    17683..17777
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    17823..17917
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        17948..18186
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             complement(18260..18289)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(18298..18954)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     regulatory      18951..18960
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(18973..19350)
                     /codon_start=1
                     /gene="HIST1H2BB"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPSKSAPAPKKGSKKAITKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     primer_bind     complement(20193..20211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgacgc tagtcctaac atgcctgcag gtcggagtac tgtcctccga gcggagtact
     6541 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     6601 ctccgagcgg agactctagc cctagcgacg tcaagcttgc atgcctgcag gtcggagtac
     6661 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     6721 cctccgagcg gagtactgtc ctccgagcgg agactctagc cctagcgacg tcgagcgccg
     6781 gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga
     6841 acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc
     6901 tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa
     6961 ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata
     7021 gatcagaagt tcctattctt caaatagtat aggaacttcg atctcgatgc cgtgcggcca
     7081 tactagtcga acctatgcag atcctcttct gagatgagtt tttgttcgcg gccgctttac
     7141 ttgtacagct cgtccatgcc gggagttatc ccggcggcgg taacgaactc cagcaggacc
     7201 atgtgatcgc gctgctcgtt ggggtctttg aacagcttgc tctggaagct caggtagtgg
     7261 ttgtcaggca gcagcacgga gccgtcgccg atgggggtgt tctgctggta cgcgtcggcg
     7321 agctgcacgc cgccgtcctt gacgttgtgc tcgatcttga agttcgcttt gatgccgttc
     7381 ttctgcttgt cggccgtgat atacgcgtcc cacgcgttca tgttgtactc cagcttgtgc
     7441 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     7501 gtgtcgccct cgaacttcac ctcggcgcgg gtcctgtagc tgccgtcgtc cttgaaggag
     7561 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     7621 atgtggtcgg ggtagcgggc gaagcactgc acgccgtaag acagggtggt cacgagggtg
     7681 ggccagggca cgggcagctc gccggaggtg cagatgaact tcagggtcag cttgccgttg
     7741 gtggcatcgc cctcgccctc gccgcgcacg ctgaacctgt ggccgtttac gtcgccgtcc
     7801 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct gaccatggtg
     7861 gcgaccggtg gatccttaga gctagtgtac ttggtaactg ccttagtgcc ctcggacaca
     7921 gcatgcttag ccagctcccc aggcagcagc aggcgcacag ccgtctgaat ctccctggag
     7981 gtgatggtcg agcgcttatt gtagtgagcc aggcgagaag cctcgcccgc gatgcgctcg
     8041 aagatgtcgt tgacgaagga gttcatgatc cccatggcct tggatgagat gccggtgtcg
     8101 gggtggacct gcttcagaac cttgtacaca tagatagaat agctctcctt gcggctgcgc
     8161 ttacgcttct taccatcctt cttctgcgcc ttagtgatag ccttcttaga acccttttta
     8221 ggggctggag cagacttaga gggttcaggc atggtggcgg taccctcgag ccagatctgc
     8281 tagcactagg gtctggtcgg cctggaagtt cctatactat ttgaagaata ggaacttcgt
     8341 cgagggtacc tctagaatga atcgttttta aaataacaaa tcaattgttt tataatattc
     8401 gtacgattct ttgattatgt aataaaatgt gatcattagg aagattacga aaaatataaa
     8461 aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa
     8521 taaacccatg attatttgat taaaattgtt gttttctttg ttcatagaca atagtgtgtt
     8581 ttgcctaaac gtgtactgca taaactccat gcgagtgtat agcgagctag tggctaacgc
     8641 ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa
     8701 catttggccg tcggaattaa cttctaaaga tgccacataa tctaataaat gaaatagaga
     8761 ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg gcataaactc
     8821 tatgatttct ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg
     8881 cgacatgtcg tcgggaaact cgcgcggaaa catgttgttg taaccgaacg ggtcccatag
     8941 cgccaaaacc aaatctgcca gcgtcaatag aatgagcacg atgccgacaa tggagctggc
     9001 ttggatagcg attcgagtta ggcatacgtt ccacacctag cttggttagc ctagccgggt
     9061 cctcaacgac aggagcacga tcatgcgcac ccgtggccag ggccgacgct agtcctaaca
     9121 tgcctgcagg tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt
     9181 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcc
     9241 ctagcgacgt caagcttgca tgcctgcagg tcggagtact gtcctccgag cggagtactg
     9301 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     9361 tccgagcgga gactctagcc ctagcgacgt cgagcgccgg agtataaata gaggcgcttc
     9421 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
     9481 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
     9541 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
     9601 aagaagtaat tattgaatac aagaagagaa ctctgaatag atcagaagtt cctattctct
     9661 agaaggtata ggaacttcga tctcgatgcc gtgctagaac tagtcgaacc tatgcagatc
     9721 ctcttctgag atgagttttt gttcgcggcc gctcaagatc tgagtccgga gctgtgcccc
     9781 agtttgctag gcaggtcgca gtacctggcc acagccatct cgtgctgctc gacgtaggtc
     9841 tctttgtcgg cctccttgat tctttccagt ctcctgtcca cgaagtagaa gccgggcatc
     9901 ttgaggttct tagcgggttt cttggatctg tatgtggtct tgagggagca gtgcaggtag
     9961 cccccgccca cgagcttcag ggccatctgg gcatggcctc tcaggccgct gtcagcgggg
    10021 tacagcatct cggtgctggc ctcccagccg agtgttttct tctgcatcac agggccgttg
    10081 gatgggaagt tcaccccgtt gatcttgacg ttgtagatga ggcagccgtt ctggaggctg
    10141 gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgatcct ctcccatgtg
    10201 aagccctcag ggaaggactg cttaaagaag tcggggatgc cctgggtgtg gttgataaag
    10261 gttttgctgc cgtacatgaa gctggtagcc aggatgtcga aggcgaaggg gagagggccg
    10321 ccctcgacca ccttgatctt catggtctgg gtgccctcgt agggcttgcc ttcgccctcg
    10381 gatgtgcact tgaagtggtg gtcgttcacg gtgccctcca tgtacagttt catgtgcatg
    10441 ttctcggtga tcagcacgct atcctcaccc accatggcca tgttgttgtc ctcggaggag
    10501 gcggtaccgg agctgccgct gccggtgctg ccggtgccat gcccgctgtg ccccagtttg
    10561 ctaggcaggt cgcagtacct ggccacagcc atctcgtgct gctcgacgta ggtctctttg
    10621 tcggcctcct tgattctttc cagtctcctg tccacgaagt agaagccggg catcttgagg
    10681 ttcttagcgg gtttcttgga tctgtatgtg gtcttgaggg agcagtgcag gtagcccccg
    10741 cccacgagct tcagggccat ctgggcatgg cctctcaggc cgctgtcagc ggggtacagc
    10801 atctcggtgc tggcctccca gccgagtgtt ttcttctgca tcacagggcc gttggatggg
    10861 aagttcaccc cgttgatctt gacgttgtag atgaggcagc cgttctggag gctggtgtcc
    10921 tgggtagcgg tcagcacgcc cccgtcttcg tatgtggtga tcctctccca tgtgaagccc
    10981 tcagggaagg actgcttaaa gaagtcgggg atgccctggg tgtggttgat aaaggttttg
    11041 ctgccgtaca tgaagctggt agccaggatg tcgaaggcga aggggagagg gccgccctcg
    11101 accaccttga tcttcatggt ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg
    11161 cacttgaagt ggtggtcgtt cacggtgccc tccatgtaca gtttcatgtg catgttctcg
    11221 gtgatcagca cgctatcctc acccaccatg gtggcgaccg gtggatcctt agagctagtg
    11281 tacttggtaa ctgccttagt gccctcggac acagcatgct tagccagctc cccaggcagc
    11341 agcaggcgca cagccgtctg aatctccctg gaggtgatgg tcgagcgctt attgtagtga
    11401 gccaggcgag aagcctcgcc cgcgatgcgc tcgaagatgt cgttgacgaa ggagttcatg
    11461 atccccatgg ccttggatga gatgccggtg tcggggtgga cctgcttcag aaccttgtac
    11521 acatagatag aatagctctc cttgcggctg cgcttacgct tcttaccatc cttcttctgc
    11581 gccttagtga tagccttctt agaacccttt ttaggggctg gagcagactt agagggttca
    11641 ggcatggtgg cggtaccctc gagccagatc tgctagcaga tccgtctggt cggcctggaa
    11701 gttcctatac cttctagaga ataggaactt cgtcgagggt acctctagaa tgaatcgttt
    11761 ttaaaataac aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa
    11821 tgtgatcatt aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa
    11881 tgctgtaaac gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt
    11941 gttgttttct ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc
    12001 catgcgagtg tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa
    12061 atcctcaatt tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa
    12121 agatgccaca taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc
    12181 gaccatttcc gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc
    12241 gaaactctca aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg
    12301 aaacatgttg ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa
    12361 tagaatgagc acgatgccga caatggagct ggcttggata gcgattcgag ttcatccaat
    12421 taacaccccg ccaagtgagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
    12481 ccgtggccag ggccgacgct agtcctaaca tgcctgcagg tcggagtact gtcctccgag
    12541 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
    12601 agtactgtcc tccgagcgga gactctagcc ctagcgacgt caagcttgca tgcctgcagg
    12661 tcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
    12721 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactctagcc ctagcgacgt
    12781 cgagcgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
    12841 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
    12901 taaacaatct gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa
    12961 gtaaatcaac tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa
    13021 ctctgaatag atcagaagtt cctattctct aaaaagtata ggaacttcga tctcgatgcc
    13081 gtagatcgac tagtcgaacc tatgcagatc ctcttctgag atgagttttt gttcgcggcc
    13141 gcttacttgt acagctcgtc catgcccatc acatcggtaa aggccttttg ccactccttg
    13201 aagttgagct cggtcttgga gtgcttgagc tccgtcttac ggaacacgta catcggctgg
    13261 ttcttcagat agttagccgc cattggcttg gcaaaggtgt aggtggtccg cgcagtgctc
    13321 cggtagcgct tgccatttcc agtggtgtaa ctccacttaa aggtactgat gatggttttg
    13381 tcgttggggt aagtcttctt cgacctgcac cagtccgcag cggtcagcga gttggtcatc
    13441 acaggaccgt cagcagggaa accagtcccc ttcacctggg cctctccttt gatgtggctt
    13501 ccctcgtagg tgtagcggta gttaacagta agggaggcac catcttcaaa ctgcattgtg
    13561 cgatggactt ggtagccgga gccatctacc atggcggcct ggaaaggcga catcccgtca
    13621 gggtagggca ggtactgatg gaagccatac ccgatatgag ggaccagaat ccagggggag
    13681 aactggaggt cacccttggt ggacttcagg tttaactcct cataaccatc atttggattg
    13741 ccggtgccct gacccaccat gtcaaagtcc acaccgttga tggagccaaa gatgtgtaac
    13801 tcatgtgtcg ctgggagaga ggccatgtta tcctcctcgc ccttgctcac catggtggcg
    13861 accggtggat ccttagagct agtgtacttg gtaactgcct tagtgccctc ggacacagca
    13921 tgcttagcca gctccccagg cagcagcagg cgcacagccg tctgaatctc cctggaggtg
    13981 atggtcgagc gcttattgta gtgagccagg cgagaagcct cgcccgcgat gcgctcgaag
    14041 atgtcgttga cgaaggagtt catgatcccc atggccttgg atgagatgcc ggtgtcgggg
    14101 tggacctgct tcagaacctt gtacacatag atagaatagc tctccttgcg gctgcgctta
    14161 cgcttcttac catccttctt ctgcgcctta gtgatagcct tcttagaacc ctttttaggg
    14221 gctggagcag acttagaggg ttcaggcatg gtggcggtac cctcgagcca gatctgctag
    14281 catcgaggtc tggtcggcct ggaagttcct atacttttta gagaatagga acttcgtcga
    14341 gggtacctct agaatgaatc gtttttaaaa taacaaatca attgttttat aatattcgta
    14401 cgattctttg attatgtaat aaaatgtgat cattaggaag attacgaaaa atataaaaaa
    14461 tatgagttct gtgtgtataa caaatgctgt aaacgccaca attgtgtttg ttgcaaataa
    14521 acccatgatt atttgattaa aattgttgtt ttctttgttc atagacaata gtgtgttttg
    14581 cctaaacgtg tactgcataa actccatgcg agtgtatagc gagctagtgg ctaacgcttg
    14641 ccccaccaaa gtagattcgt caaaatcctc aatttcatca ccctcctcca agtttaacat
    14701 ttggccgtcg gaattaactt ctaaagatgc cacataatct aataaatgaa atagagattc
    14761 aaacgtggcg tcatcgtccg tttcgaccat ttccgaaaag aactcgggca taaactctat
    14821 gatttctctg gacgtggtgt tgtcgaaact ctcaaagtac gcagtcagga acgtgcgcga
    14881 catgtcgtcg ggaaactcgc gcggaaacat gttgttgtaa ccgaacgggt cccatagcgc
    14941 caaaaccaaa tctgccagcg tcaatagaat gagcacgatg ccgacaatgg agctggcttg
    15001 gatagcgatt cgagttaacg taccctacaa catgcaaacc cgccgtttga cgctagtcct
    15061 aacatgcctg caggtcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
    15121 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactct
    15181 agccctagcg acgtcaagct tgcatgcctg caggtcggag tactgtcctc cgagcggagt
    15241 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
    15301 gtcctccgag cggagactct agccctagcg acgtcgagcg ccggagtata aatagaggcg
    15361 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
    15421 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
    15481 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
    15541 tgccaagaag taattattga atacaagaag agaactctga atagatcaga agttcctatt
    15601 ctatcagaag tataggaact tcgattcgat gccgtaccgg cactagtcga acctatgcag
    15661 atcctcttct gagatgagtt tttgttcgcg gccgctttac ttgtacagct cgtccatgcc
    15721 gtcggtggag ttgcgggcca cggcgctctc gtaaacggtc accttgttgt agtccttgtc
    15781 gtggttcagg atctcgatgc ggtggtccac aaagtgatag tcgggcagct tcaccgcctt
    15841 cttggccctg tagatggtct tgaagtcaac gcggtggtgg ccgccgccct ccagcagcag
    15901 cttgtgcttg acgtcgccct tcagcacgcc gtcgcgcacg tacatcctct cggtggaggc
    15961 gtcccagccg gtggtcttct tctgcatcac ggggccgttg ggggggaagt tctcgccctt
    16021 gaggtgtatc tcgtagatga aggagtcctc ctccatggag atgtcggact tcaccttcac
    16081 gatgcccttg tcctcgaagg tcatggtgcg ctcccaagag tagccctcgg ggaaggactg
    16141 cttgaagtag ttggggatgt cgtcggggta cttggtgaag gccctgttgc cgtaggcgaa
    16201 cgcggtggtc agaatgtcgt aggagaaggg caggggggct ccctccttca cctccaggtt
    16261 gatggtgttg gtgccgtcgt agggcttgcc ctcgccctcg ccctcgatca cgaaggcgtg
    16321 gccattcacg ttgccctcca tcttcagctt gatcttcatg tcgggcttga ttacgcccat
    16381 tgtggtctcc tcgcccttgc tgaccatggt ggcgaccggt ggatccttag agctagtgta
    16441 cttggtaact gccttagtgc cctcggacac agcatgctta gccagctccc caggcagcag
    16501 caggcgcaca gccgtctgaa tctccctgga ggtgatggtc gagcgcttat tgtagtgagc
    16561 caggcgagaa gcctcgcccg cgatgcgctc gaagatgtcg ttgacgaagg agttcatgat
    16621 ccccatggcc ttggatgaga tgccggtgtc ggggtggacc tgcttcagaa ccttgtacac
    16681 atagatagaa tagctctcct tgcggctgcg cttacgcttc ttaccatcct tcttctgcgc
    16741 cttagtgata gccttcttag aacccttttt aggggctgga gcagacttag agggttcagg
    16801 catggtggcg gtaccctcga gccagatctg ctagcgagtc cggtctggtc ggctggaagt
    16861 tcctatactt ctgatagaat aggaacttcg tcgagggtac ctctagaatg aatcgttttt
    16921 aaaataacaa atcaattgtt ttataatatt cgtacgattc tttgattatg taataaaatg
    16981 tgatcattag gaagattacg aaaaatataa aaaatatgag ttctgtgtgt ataacaaatg
    17041 ctgtaaacgc cacaattgtg tttgttgcaa ataaacccat gattatttga ttaaaattgt
    17101 tgttttcttt gttcatagac aatagtgtgt tttgcctaaa cgtgtactgc ataaactcca
    17161 tgcgagtgta tagcgagcta gtggctaacg cttgccccac caaagtagat tcgtcaaaat
    17221 cctcaatttc atcaccctcc tccaagttta acatttggcc gtcggaatta acttctaaag
    17281 atgccacata atctaataaa tgaaatagag attcaaacgt ggcgtcatcg tccgtttcga
    17341 ccatttccga aaagaactcg ggcataaact ctatgatttc tctggacgtg gtgttgtcga
    17401 aactctcaaa gtacgcagtc aggaacgtgc gcgacatgtc gtcgggaaac tcgcgcggaa
    17461 acatgttgtt gtaaccgaac gggtcccata gcgccaaaac caaatctgcc agcgtcaata
    17521 gaatgagcac gatgccgaca atggagctgg cttggatagc gattcgagtt aggcatacgt
    17581 tccacaccta gcttggttag cctagccggg tcctcaacga caggagcacg atcatgcgca
    17641 cccgtggcca gggccgacgc tagtcctaac atgcctgcag gtcggagtac tgtcctccga
    17701 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
    17761 gagtactgtc ctccgagcgg agactctagc cctagcgacg tcaagcttgc atgcctgcag
    17821 gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
    17881 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactctagc cctagcgacg
    17941 tcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
    18001 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
    18061 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
    18121 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
    18181 actctgaata gatccgaagt tcctattctc atataagtat aggaacttcg atctcgatgc
    18241 cgtactagtc gaacctatgc agatcctctt ctgagatgag tttttgttcg cggccgctta
    18301 ggagtgggcc acggcgtcct ctacctgctc agtaatgttg ccttcggttt tcctgacgag
    18361 gcgatggccg atgtaatggt ctcctggcat ttcaagaatc tcttttgccg ccttgtaagt
    18421 agtcttcatt tggcatttgt gattgccgcc tccttcaagt tttaggtaca tcgtaacatc
    18481 acccttcaga actccgtcgc tggcagtaat tttctcggtt gatggctccc aatcaacact
    18541 ttggttttgc atgataggac catcggcagg aaagttaacc ccagtaaatt tggatttgtg
    18601 gtagaaggtg tttcctctaa ggcttatatg cgcactgact gaagcggacc caccatcttc
    18661 gaactccaac gacctttccc atgacaggcc ttcaggaaat gcttgtttga aatagtctgg
    18721 tatctcttct ggatatttgg taaaaactct gtggccgtaa cagaacacgt gggacactaa
    18781 gtcaaacgcg aaaggcattg gcccgccctc ggccattgtg acgcgtagtg tcatctcttg
    18841 atgtccctcg taaggtctgc ctgtgccttc accttcaatt gtgaactcat gcccattgac
    18901 ggagccgtcc atgtagtacc tcatcttcat ctcgggcttg atcacgctca ccatggtggc
    18961 gaccggtgga tccttagagc tagtgtactt ggtaactgcc ttagtgccct cggacacagc
    19021 atgcttagcc agctccccag gcagcagcag gcgcacagcc gtctgaatct ccctggaggt
    19081 gatggtcgag cgcttattgt agtgagccag gcgagaagcc tcgcccgcga tgcgctcgaa
    19141 gatgtcgttg acgaaggagt tcatgatccc catggccttg gatgagatgc cggtgtcggg
    19201 gtggacctgc ttcagaacct tgtacacata gatagaatag ctctccttgc ggctgcgctt
    19261 acgcttctta ccatccttct tctgcgcctt agtgatagcc ttcttagaac cctttttagg
    19321 ggctggagca gacttagagg gttcaggcat ggtggcggta ccctcgagcc agatctgcta
    19381 gcgtctggtc ggcctggaag ttcctatact tatatgagaa taggaacttc gtcgagggta
    19441 cctctagaat gaatcgtttt taaaataaca aatcaattgt tttataatat tcgtacgatt
    19501 ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata aaaaatatga
    19561 gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca aataaaccca
    19621 tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg ttttgcctaa
    19681 acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac gcttgcccca
    19741 ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt aacatttggc
    19801 cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga gattcaaacg
    19861 tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac tctatgattt
    19921 ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg cgcgacatgt
    19981 cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat agcgccaaaa
    20041 ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg gcttggatag
    20101 cgattcgagt taacgtaccc tacaacatgc aaacccgccg tttaaacaac gagcttcatc
    20161 ctactccaac cggaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
    20221 taatgtcatg ataataatgg tttctta
//
