LOCUS       sequence-267731-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (F733 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 F733 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        172..551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        552..755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     706..726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     800..819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        800..818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3043..3063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3079..3099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4708..4755
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4756..4779
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4789..4845
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4846..5244
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5315..5332)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5321..5545
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5591..6019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5678..5697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5888..5909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6028..6048)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6033..6362
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6213..6348
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6275..6294
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6429..7223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6483..6502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7093..7112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7397..7518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7434..7453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7488..7507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7567..7583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7567..7583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7580..7602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7591..7607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7615..7621,7622..7639,7640..7645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7660..7681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7798..7815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7969..8557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8049..8068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8728..9519,9520..9588))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9351..9370
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9589..9693)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9768..9787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
       61 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      721 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
      781 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgcc
      841 accatgaagc gaaattacat tctggggctg gccattggca ttacatcagt gggctatggc
      901 atcattgact acgagacaag ggacgtgatc gacgccggcg tgagactgtt caaggaggcc
      961 aacgtggaga acaatgaggg ccggagatcc aagaggggag caaggcgcct gaagcggaga
     1021 aggcgccaca gaatccagag agtgaagaag ctgctgttcg attacaacct gctgaccgac
     1081 cactccgagc tgtctggcat caatccttat gaggccagag tgaagggcct gtcccagaag
     1141 ctgtctgagg aggagtttag cgccgccctg ctgcacctgg caaagaggag aggcgtgcac
     1201 aacgtgaatg aggtggagga ggacaccggc aacgagctgt ccacaaagga gcagatcagc
     1261 cgcaattcca aggccctgga ggagaagtat gtggccgagc tgcagctgga gcggctgaag
     1321 aaggatggcg aggtgagggg ctccatcaat cgcttcaaga cctctgacta cgtgaaggag
     1381 gccaagcagc tgctgaaggt gcagaaggcc taccaccagc tggatcagtc ctttatcgat
     1441 acatatatcg acctgctgga gacaaggcgc acatactatg agggaccagg agagggctct
     1501 cccttcggct ggaaggacat caaggagtgg tacgagatgc tgatgggcca ctgcacctat
     1561 tttccagagg agctgagaag cgtgaagtac gcctataacg ccgatctgta caacgccctg
     1621 aatgacctga acaacctggt catcaccagg gatgagaacg agaagctgga gtactatgag
     1681 aagttccaga tcatcgagaa cgtgttcaag cagaagaaga agcctacact gaagcagatc
     1741 gccaaggaga tcctggtgaa cgaggaggac atcaagggct accgcgtgac ctccacaggc
     1801 aagccagagt tcaccaatct gaaggtgtat cacgatatca aggacatcac agcccggaag
     1861 gagatcatcg agaacgccga gctgctggat cagatcgcca agatcctgac catctatcag
     1921 agctccgagg acatccagga ggagctgacc aacctgaata gcgagctgac acaggaggag
     1981 atcgagcaga tcagcaatct gaagggctac accggcacac acaacctgag cctgaaggcc
     2041 atcaatctga tcctggatga gctgtggcac acaaacgaca atcagatcgc catctttaac
     2101 cggctgaagc tggtgccaaa gaaggtggac ctgtcccagc agaaggagat cccaaccaca
     2161 ctggtggacg atttcatcct gtctcccgtg gtgaagcgga gcttcatcca gagcatcaaa
     2221 gtgatcaacg ccatcatcaa gaagtacggc ctgcccaatg atatcatcat cgagctggcc
     2281 agggagaaga actccaagga cgcccagaag atgatcaatg agatgcagaa gaggaaccgc
     2341 cagaccaatg agcggatcga ggagatcatc agaaccacag gcaaggagaa cgccaagtac
     2401 ctgatcgaga agatcaagct gcacgatatg caggagggca agtgtctgta ttctctggag
     2461 gccatccctc tggaggacct gctgaacaat ccattcaact acgaggtgga tcacatcatc
     2521 ccccggagcg tgagcttcga caattctttt aacaataagg tgctggtgaa gcaggaggag
     2581 aacagcaaga agggcaatag gacccctttc cagtacctgt ctagctccga ttctaagatc
     2641 agctacgaga cattcaagaa gcacatcctg aatctggcca agggcaaggg ccgcatcagc
     2701 aagaccaaga aggagtacct gctggaggag cgggacatca acagattctc cgtgcagaag
     2761 gacttcatca accggaatct ggtggacacc agatacgcca cacgcggcct gatgaatctg
     2821 ctgcggtctt atttcagagt gaacaatctg gatgtgaagg tgaagagcat caacggcggc
     2881 ttcacctcct ttctgcggag aaagtggaag tttaagaagg agcgcaacaa gggctataag
     2941 caccacgccg aggatgccct gatcatcgcc aatgccgact tcatctttaa ggagtggaag
     3001 aagctggaca aggccaagaa agtgatggag aaccagatgt tcccgaagaa gaaacgtaag
     3061 gtgggtagca gcggtagccc gaaaaagaag cgcaaagtgg gcagcgacgc gctggacgat
     3121 ttcgatctgg acatgctggg cagcgatgcg ctggatgatt tcggcggtgg cagctctgag
     3181 gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc
     3241 gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag
     3301 ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg
     3361 agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacggcaca
     3421 ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg
     3481 tttggcgtga ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac
     3541 cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc
     3601 ctgctgtgct atttctttcg gatgcctaga caggtgttca atgctcagaa gaaggcccag
     3661 agctccaccg acggcggtag cggcggtagc ggtggtagcg gcggcagcgg tggtagcggt
     3721 ggcagcggtg gtttcgagga gaagcaggcc gagagcatgc ccgagatcga gacagagcag
     3781 gagtacaagg agattttcat cacacctcac cagatcaagc acatcaagga cttcaaggac
     3841 tacaagtatt ctcacagggt ggataagaag cccaaccgcg agctgatcaa tgacaccctg
     3901 tatagcacac ggaaggacga taagggcaat accctgatcg tgaacaatct gaacggcctg
     3961 tacgacaagg ataatgacaa gctgaagaag ctgatcaaca agtctcccga gaagctgctg
     4021 atgtaccacc acgatcctca gacatatcag aagctgaagc tgatcatgga gcagtacggc
     4081 gacgagaaga acccactgta taagtactat gaggagacag gcaactacct gacaaagtat
     4141 agcaagaagg ataatggccc cgtgatcaag aagatcaagt actatggcaa caagctgaat
     4201 gcccacctgg acatcaccga cgattaccct aactctcgca ataaggtggt gaagctgagc
     4261 ctgaagccat accggttcga cgtgtacctg gacaacggcg tgtataagtt tgtgacagtg
     4321 aagaatctgg atgtgatcaa gaaggagaac tactatgagg tgaacagcaa gtgctacgag
     4381 gaggccaaga agctgaagaa gatcagcaac caggccgagt tcatcgcctc tttttacaac
     4441 aatgacctga tcaagatcaa tggcgagctg tatagagtga tcggcgtgaa caatgatctg
     4501 ctgaacagaa tcgaagtgaa tatgatcgac atcacctaca gggagtatct ggagaacatg
     4561 aatgataaga ggccccctcg catcatcaag accatcgcct ctaagacaca gagcatcaag
     4621 aagtacagca cagacatcct ggggaacctg tatgaagtca agagcaagaa acatcctcag
     4681 attatcaaga aaggctctgg tggttctaag cgacctgccg ccacaaagaa ggctggacag
     4741 gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc aacaaacttc
     4801 tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatggc caagcctttg
     4861 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     4921 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     4981 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     5041 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     5101 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     5161 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     5221 tctggttatg tgtgggaggg ctaagaattc tgcagatatc cagcacagtg gcggccgctc
     5281 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5341 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5401 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5461 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     5521 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     5581 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5641 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5701 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5761 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5821 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5881 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5941 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6001 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6061 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6121 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6181 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     6241 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     6301 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     6361 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     6421 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     6481 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     6541 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     6601 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     6661 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     6721 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     6781 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     6841 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6901 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6961 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7021 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7081 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7141 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7201 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     7261 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     7321 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     7381 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7441 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7501 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     7561 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7621 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7681 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7741 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7801 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7861 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7921 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7981 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8041 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8101 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8161 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8221 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8281 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8341 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8401 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8461 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8521 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8581 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8641 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8701 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8761 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8821 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8881 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8941 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9001 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9061 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9121 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9181 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9241 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9301 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9361 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9421 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9481 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9541 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9601 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9661 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9721 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     9781 gatgccg
//
