LOCUS       sequence-267732-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E125 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E125 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     782..801
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        782..800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1204..1224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1240..1260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4690..4737
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4738..4761
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4771..4827
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4828..5226
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5297..5314)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5303..5527
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5573..6001
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5660..5679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5870..5891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6010..6030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6015..6344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6195..6330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6257..6276
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6411..7205
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6465..6484)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7075..7094
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7379..7500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7416..7435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7470..7489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7549..7565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7549..7565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7562..7584)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7573..7589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7597..7603,7604..7621,7622..7627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7642..7663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7780..7797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7951..8539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8031..8050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8710..9501,9502..9570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9333..9352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9571..9675)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9750..9769)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
       61 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      121 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa
      781 ttaatacgac tcactatagg gagacccaag ctggctagcg ccaccatgaa gcgaaattac
      841 attctggggc tggccattgg cattacatca gtgggctatg gcatcattga ctacgagaca
      901 agggacgtga tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga gaacaatgag
      961 ggccggagat ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca cagaatccag
     1021 agagtgaaga agctgctgtt cgattacaac ctgctgaccg accactccga gctgtctggc
     1081 atcaatcctt atgaggccag agtgaagggc ctgtcccaga agctgtctga ggaggagttt
     1141 agcgccgccc tgctgcacct ggcaaagagg agaggcgtgc acaacgtgaa tgaggtggag
     1201 gagccgaaga agaaacgtaa ggtgggtagc agcggtagcc cgaaaaagaa gcgcaaagtg
     1261 ggcagcgacg cgctggacga tttcgatctg gacatgctgg gcagcgatgc gctggatgat
     1321 ttcggcggtg gcagctctga ggtggagttt tcccacgagt actggatgag acatgccctg
     1381 accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg
     1441 aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc
     1501 catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt
     1561 gacgccaccc tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc catgatccac
     1621 tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc
     1681 ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc
     1741 ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc
     1801 aatgctcaga agaaggccca gagctccacc gacggcggta gcggcggtag cggtggtagc
     1861 ggcggcagcg gtggtagcgg tggcagcggt ggtgaggaca ccggcaacga gctgtccaca
     1921 aaggagcaga tcagccgcaa ttccaaggcc ctggaggaga agtatgtggc cgagctgcag
     1981 ctggagcggc tgaagaagga tggcgaggtg aggggctcca tcaatcgctt caagacctct
     2041 gactacgtga aggaggccaa gcagctgctg aaggtgcaga aggcctacca ccagctggat
     2101 cagtccttta tcgatacata tatcgacctg ctggagacaa ggcgcacata ctatgaggga
     2161 ccaggagagg gctctccctt cggctggaag gacatcaagg agtggtacga gatgctgatg
     2221 ggccactgca cctattttcc agaggagctg agaagcgtga agtacgccta taacgccgat
     2281 ctgtacaacg ccctgaatga cctgaacaac ctggtcatca ccagggatga gaacgagaag
     2341 ctggagtact atgagaagtt ccagatcatc gagaacgtgt tcaagcagaa gaagaagcct
     2401 acactgaagc agatcgccaa ggagatcctg gtgaacgagg aggacatcaa gggctaccgc
     2461 gtgacctcca caggcaagcc agagttcacc aatctgaagg tgtatcacga tatcaaggac
     2521 atcacagccc ggaaggagat catcgagaac gccgagctgc tggatcagat cgccaagatc
     2581 ctgaccatct atcagagctc cgaggacatc caggaggagc tgaccaacct gaatagcgag
     2641 ctgacacagg aggagatcga gcagatcagc aatctgaagg gctacaccgg cacacacaac
     2701 ctgagcctga aggccatcaa tctgatcctg gatgagctgt ggcacacaaa cgacaatcag
     2761 atcgccatct ttaaccggct gaagctggtg ccaaagaagg tggacctgtc ccagcagaag
     2821 gagatcccaa ccacactggt ggacgatttc atcctgtctc ccgtggtgaa gcggagcttc
     2881 atccagagca tcaaagtgat caacgccatc atcaagaagt acggcctgcc caatgatatc
     2941 atcatcgagc tggccaggga gaagaactcc aaggacgccc agaagatgat caatgagatg
     3001 cagaagagga accgccagac caatgagcgg atcgaggaga tcatcagaac cacaggcaag
     3061 gagaacgcca agtacctgat cgagaagatc aagctgcacg atatgcagga gggcaagtgt
     3121 ctgtattctc tggaggccat ccctctggag gacctgctga acaatccatt caactacgag
     3181 gtggatcaca tcatcccccg gagcgtgagc ttcgacaatt cttttaacaa taaggtgctg
     3241 gtgaagcagg aggagaacag caagaagggc aataggaccc ctttccagta cctgtctagc
     3301 tccgattcta agatcagcta cgagacattc aagaagcaca tcctgaatct ggccaagggc
     3361 aagggccgca tcagcaagac caagaaggag tacctgctgg aggagcggga catcaacaga
     3421 ttctccgtgc agaaggactt catcaaccgg aatctggtgg acaccagata cgccacacgc
     3481 ggcctgatga atctgctgcg gtcttatttc agagtgaaca atctggatgt gaaggtgaag
     3541 agcatcaacg gcggcttcac ctcctttctg cggagaaagt ggaagtttaa gaaggagcgc
     3601 aacaagggct ataagcacca cgccgaggat gccctgatca tcgccaatgc cgacttcatc
     3661 tttaaggagt ggaagaagct ggacaaggcc aagaaagtga tggagaacca gatgttcgag
     3721 gagaagcagg ccgagagcat gcccgagatc gagacagagc aggagtacaa ggagattttc
     3781 atcacacctc accagatcaa gcacatcaag gacttcaagg actacaagta ttctcacagg
     3841 gtggataaga agcccaaccg cgagctgatc aatgacaccc tgtatagcac acggaaggac
     3901 gataagggca ataccctgat cgtgaacaat ctgaacggcc tgtacgacaa ggataatgac
     3961 aagctgaaga agctgatcaa caagtctccc gagaagctgc tgatgtacca ccacgatcct
     4021 cagacatatc agaagctgaa gctgatcatg gagcagtacg gcgacgagaa gaacccactg
     4081 tataagtact atgaggagac aggcaactac ctgacaaagt atagcaagaa ggataatggc
     4141 cccgtgatca agaagatcaa gtactatggc aacaagctga atgcccacct ggacatcacc
     4201 gacgattacc ctaactctcg caataaggtg gtgaagctga gcctgaagcc ataccggttc
     4261 gacgtgtacc tggacaacgg cgtgtataag tttgtgacag tgaagaatct ggatgtgatc
     4321 aagaaggaga actactatga ggtgaacagc aagtgctacg aggaggccaa gaagctgaag
     4381 aagatcagca accaggccga gttcatcgcc tctttttaca acaatgacct gatcaagatc
     4441 aatggcgagc tgtatagagt gatcggcgtg aacaatgatc tgctgaacag aatcgaagtg
     4501 aatatgatcg acatcaccta cagggagtat ctggagaaca tgaatgataa gaggccccct
     4561 cgcatcatca agaccatcgc ctctaagaca cagagcatca agaagtacag cacagacatc
     4621 ctggggaacc tgtatgaagt caagagcaag aaacatcctc agattatcaa gaaaggctct
     4681 ggtggttcta agcgacctgc cgccacaaag aaggctggac aggctaagaa gaagaaagat
     4741 tacaaagacg atgacgataa gggatccggc gcaacaaact tctctctgct gaaacaagcc
     4801 ggagatgtcg aagagaatcc tggaccgatg gccaagcctt tgtctcaaga agaatccacc
     4861 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     4921 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     4981 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     5041 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     5101 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     5161 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     5221 ggctaagaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     5281 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5341 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5401 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5461 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5521 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     5581 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5641 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5701 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5761 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5821 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5881 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5941 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6001 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6061 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6121 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6181 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6241 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     6301 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     6361 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     6421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     6601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7201 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7261 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7321 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7381 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7441 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7501 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7561 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7621 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7681 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7741 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7801 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7861 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7921 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7981 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8041 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8101 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8161 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8221 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8281 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8341 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8401 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8461 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8521 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8581 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8641 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8701 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8761 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8821 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8881 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8941 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9001 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9061 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9121 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9181 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9241 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9301 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9361 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9421 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9481 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9541 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9601 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9661 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9721 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9781 cagtatc
//
