LOCUS       sequence-267733-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (M732 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 M732 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        175..554
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        555..758
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     709..729
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     803..822
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        803..821
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3043..3063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3079..3099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4711..4758
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4759..4782
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4792..4848
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4849..5247
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5318..5335)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5324..5548
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5594..6022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5681..5700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5891..5912
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6031..6051)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6036..6365
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6216..6351
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6278..6297
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6432..7226
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6486..6505)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7096..7115
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7400..7521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7437..7456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7491..7510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7570..7586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7570..7586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7583..7605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7594..7610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7618..7624,7625..7642,7643..7648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7663..7684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7801..7818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7972..8560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8052..8071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8731..9522,9523..9591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9354..9373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9592..9696)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9771..9787,1..3))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
       61 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      121 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      181 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      241 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      301 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      361 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      421 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      481 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      541 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      601 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      661 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      721 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      781 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      841 gccaccatga agcgaaatta cattctgggg ctggccattg gcattacatc agtgggctat
      901 ggcatcattg actacgagac aagggacgtg atcgacgccg gcgtgagact gttcaaggag
      961 gccaacgtgg agaacaatga gggccggaga tccaagaggg gagcaaggcg cctgaagcgg
     1021 agaaggcgcc acagaatcca gagagtgaag aagctgctgt tcgattacaa cctgctgacc
     1081 gaccactccg agctgtctgg catcaatcct tatgaggcca gagtgaaggg cctgtcccag
     1141 aagctgtctg aggaggagtt tagcgccgcc ctgctgcacc tggcaaagag gagaggcgtg
     1201 cacaacgtga atgaggtgga ggaggacacc ggcaacgagc tgtccacaaa ggagcagatc
     1261 agccgcaatt ccaaggccct ggaggagaag tatgtggccg agctgcagct ggagcggctg
     1321 aagaaggatg gcgaggtgag gggctccatc aatcgcttca agacctctga ctacgtgaag
     1381 gaggccaagc agctgctgaa ggtgcagaag gcctaccacc agctggatca gtcctttatc
     1441 gatacatata tcgacctgct ggagacaagg cgcacatact atgagggacc aggagagggc
     1501 tctcccttcg gctggaagga catcaaggag tggtacgaga tgctgatggg ccactgcacc
     1561 tattttccag aggagctgag aagcgtgaag tacgcctata acgccgatct gtacaacgcc
     1621 ctgaatgacc tgaacaacct ggtcatcacc agggatgaga acgagaagct ggagtactat
     1681 gagaagttcc agatcatcga gaacgtgttc aagcagaaga agaagcctac actgaagcag
     1741 atcgccaagg agatcctggt gaacgaggag gacatcaagg gctaccgcgt gacctccaca
     1801 ggcaagccag agttcaccaa tctgaaggtg tatcacgata tcaaggacat cacagcccgg
     1861 aaggagatca tcgagaacgc cgagctgctg gatcagatcg ccaagatcct gaccatctat
     1921 cagagctccg aggacatcca ggaggagctg accaacctga atagcgagct gacacaggag
     1981 gagatcgagc agatcagcaa tctgaagggc tacaccggca cacacaacct gagcctgaag
     2041 gccatcaatc tgatcctgga tgagctgtgg cacacaaacg acaatcagat cgccatcttt
     2101 aaccggctga agctggtgcc aaagaaggtg gacctgtccc agcagaagga gatcccaacc
     2161 acactggtgg acgatttcat cctgtctccc gtggtgaagc ggagcttcat ccagagcatc
     2221 aaagtgatca acgccatcat caagaagtac ggcctgccca atgatatcat catcgagctg
     2281 gccagggaga agaactccaa ggacgcccag aagatgatca atgagatgca gaagaggaac
     2341 cgccagacca atgagcggat cgaggagatc atcagaacca caggcaagga gaacgccaag
     2401 tacctgatcg agaagatcaa gctgcacgat atgcaggagg gcaagtgtct gtattctctg
     2461 gaggccatcc ctctggagga cctgctgaac aatccattca actacgaggt ggatcacatc
     2521 atcccccgga gcgtgagctt cgacaattct tttaacaata aggtgctggt gaagcaggag
     2581 gagaacagca agaagggcaa taggacccct ttccagtacc tgtctagctc cgattctaag
     2641 atcagctacg agacattcaa gaagcacatc ctgaatctgg ccaagggcaa gggccgcatc
     2701 agcaagacca agaaggagta cctgctggag gagcgggaca tcaacagatt ctccgtgcag
     2761 aaggacttca tcaaccggaa tctggtggac accagatacg ccacacgcgg cctgatgaat
     2821 ctgctgcggt cttatttcag agtgaacaat ctggatgtga aggtgaagag catcaacggc
     2881 ggcttcacct cctttctgcg gagaaagtgg aagtttaaga aggagcgcaa caagggctat
     2941 aagcaccacg ccgaggatgc cctgatcatc gccaatgccg acttcatctt taaggagtgg
     3001 aagaagctgg acaaggccaa gaaagtgatg gagaaccaga tgccgaagaa gaaacgtaag
     3061 gtgggtagca gcggtagccc gaaaaagaag cgcaaagtgg gcagcgacgc gctggacgat
     3121 ttcgatctgg acatgctggg cagcgatgcg ctggatgatt tcggcggtgg cagctctgag
     3181 gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc
     3241 gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag
     3301 ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg
     3361 agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacggcaca
     3421 ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg
     3481 tttggcgtga ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac
     3541 cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc
     3601 ctgctgtgct atttctttcg gatgcctaga caggtgttca atgctcagaa gaaggcccag
     3661 agctccaccg acggcggtag cggcggtagc ggtggtagcg gcggcagcgg tggtagcggt
     3721 ggcagcggtg gtatgttcga ggagaagcag gccgagagca tgcccgagat cgagacagag
     3781 caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag
     3841 gactacaagt attctcacag ggtggataag aagcccaacc gcgagctgat caatgacacc
     3901 ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc
     3961 ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg
     4021 ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac
     4081 ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag
     4141 tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg
     4201 aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg
     4261 agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca
     4321 gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac
     4381 gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac
     4441 aacaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat
     4501 ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac
     4561 atgaatgata agaggccccc tcgcatcatc aagaccatcg cctctaagac acagagcatc
     4621 aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct
     4681 cagattatca agaaaggctc tggtggttct aagcgacctg ccgccacaaa gaaggctgga
     4741 caggctaaga agaagaaaga ttacaaagac gatgacgata agggatccgg cgcaacaaac
     4801 ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgat ggccaagcct
     4861 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
     4921 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
     4981 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
     5041 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
     5101 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
     5161 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
     5221 ccctctggtt atgtgtggga gggctaagaa ttctgcagat atccagcaca gtggcggccg
     5281 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     5341 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     5401 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     5461 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     5521 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5581 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6001 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6061 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6181 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6241 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6301 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6361 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     6421 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     6481 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     6541 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     6601 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6661 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6721 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6781 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6841 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6901 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     6961 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     7021 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     7081 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     7141 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     7201 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     7261 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7321 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7381 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7441 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7501 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7561 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7621 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     7681 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     7741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8401 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9601 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9661 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9721 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     9781 tctgatg
//
