LOCUS       sequence-267734-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E743 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E743 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        89..468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        469..672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     623..643
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     717..736
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        717..735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2990..3010
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3026..3046
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4625..4672
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4673..4696
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4706..4762
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4763..5161
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5232..5249)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5238..5462
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5508..5936
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5595..5614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5805..5826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5945..5965)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5950..6279
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6130..6265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6192..6211
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6346..7140
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6400..6419)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7010..7029
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7314..7435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7351..7370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7405..7424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7484..7500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7484..7500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7497..7519)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7508..7524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7532..7538,7539..7556,7557..7562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7577..7598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7715..7732)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7886..8474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7966..7985)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8645..9436,9437..9505))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9268..9287
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9506..9610)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9685..9704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
       61 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      121 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      181 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      241 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      301 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      361 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      421 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      481 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      541 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      601 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      661 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      721 acgactcact atagggagac ccaagctggc tagcgccacc atgaagcgaa attacattct
      781 ggggctggcc attggcatta catcagtggg ctatggcatc attgactacg agacaaggga
      841 cgtgatcgac gccggcgtga gactgttcaa ggaggccaac gtggagaaca atgagggccg
      901 gagatccaag aggggagcaa ggcgcctgaa gcggagaagg cgccacagaa tccagagagt
      961 gaagaagctg ctgttcgatt acaacctgct gaccgaccac tccgagctgt ctggcatcaa
     1021 tccttatgag gccagagtga agggcctgtc ccagaagctg tctgaggagg agtttagcgc
     1081 cgccctgctg cacctggcaa agaggagagg cgtgcacaac gtgaatgagg tggaggagga
     1141 caccggcaac gagctgtcca caaaggagca gatcagccgc aattccaagg ccctggagga
     1201 gaagtatgtg gccgagctgc agctggagcg gctgaagaag gatggcgagg tgaggggctc
     1261 catcaatcgc ttcaagacct ctgactacgt gaaggaggcc aagcagctgc tgaaggtgca
     1321 gaaggcctac caccagctgg atcagtcctt tatcgataca tatatcgacc tgctggagac
     1381 aaggcgcaca tactatgagg gaccaggaga gggctctccc ttcggctgga aggacatcaa
     1441 ggagtggtac gagatgctga tgggccactg cacctatttt ccagaggagc tgagaagcgt
     1501 gaagtacgcc tataacgccg atctgtacaa cgccctgaat gacctgaaca acctggtcat
     1561 caccagggat gagaacgaga agctggagta ctatgagaag ttccagatca tcgagaacgt
     1621 gttcaagcag aagaagaagc ctacactgaa gcagatcgcc aaggagatcc tggtgaacga
     1681 ggaggacatc aagggctacc gcgtgacctc cacaggcaag ccagagttca ccaatctgaa
     1741 ggtgtatcac gatatcaagg acatcacagc ccggaaggag atcatcgaga acgccgagct
     1801 gctggatcag atcgccaaga tcctgaccat ctatcagagc tccgaggaca tccaggagga
     1861 gctgaccaac ctgaatagcg agctgacaca ggaggagatc gagcagatca gcaatctgaa
     1921 gggctacacc ggcacacaca acctgagcct gaaggccatc aatctgatcc tggatgagct
     1981 gtggcacaca aacgacaatc agatcgccat ctttaaccgg ctgaagctgg tgccaaagaa
     2041 ggtggacctg tcccagcaga aggagatccc aaccacactg gtggacgatt tcatcctgtc
     2101 tcccgtggtg aagcggagct tcatccagag catcaaagtg atcaacgcca tcatcaagaa
     2161 gtacggcctg cccaatgata tcatcatcga gctggccagg gagaagaact ccaaggacgc
     2221 ccagaagatg atcaatgaga tgcagaagag gaaccgccag accaatgagc ggatcgagga
     2281 gatcatcaga accacaggca aggagaacgc caagtacctg atcgagaaga tcaagctgca
     2341 cgatatgcag gagggcaagt gtctgtattc tctggaggcc atccctctgg aggacctgct
     2401 gaacaatcca ttcaactacg aggtggatca catcatcccc cggagcgtga gcttcgacaa
     2461 ttcttttaac aataaggtgc tggtgaagca ggaggagaac agcaagaagg gcaataggac
     2521 ccctttccag tacctgtcta gctccgattc taagatcagc tacgagacat tcaagaagca
     2581 catcctgaat ctggccaagg gcaagggccg catcagcaag accaagaagg agtacctgct
     2641 ggaggagcgg gacatcaaca gattctccgt gcagaaggac ttcatcaacc ggaatctggt
     2701 ggacaccaga tacgccacac gcggcctgat gaatctgctg cggtcttatt tcagagtgaa
     2761 caatctggat gtgaaggtga agagcatcaa cggcggcttc acctcctttc tgcggagaaa
     2821 gtggaagttt aagaaggagc gcaacaaggg ctataagcac cacgccgagg atgccctgat
     2881 catcgccaat gccgacttca tctttaagga gtggaagaag ctggacaagg ccaagaaagt
     2941 gatggagaac cagatgttcg aggagaagca ggccgagagc atgcccgagc cgaagaagaa
     3001 acgtaaggtg ggtagcagcg gtagcccgaa aaagaagcgc aaagtgggca gcgacgcgct
     3061 ggacgatttc gatctggaca tgctgggcag cgatgcgctg gatgatttcg gcggtggcag
     3121 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     3181 ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     3241 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     3301 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     3361 cggcacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     3421 cgtggtgttt ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct
     3481 gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     3541 tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa
     3601 ggcccagagc tccaccgacg gcggtagcgg cggtagcggt ggtagcggcg gcagcggtgg
     3661 tagcggtggc agcggtggtg agatcgagac agagcaggag tacaaggaga ttttcatcac
     3721 acctcaccag atcaagcaca tcaaggactt caaggactac aagtattctc acagggtgga
     3781 taagaagccc aaccgcgagc tgatcaatga caccctgtat agcacacgga aggacgataa
     3841 gggcaatacc ctgatcgtga acaatctgaa cggcctgtac gacaaggata atgacaagct
     3901 gaagaagctg atcaacaagt ctcccgagaa gctgctgatg taccaccacg atcctcagac
     3961 atatcagaag ctgaagctga tcatggagca gtacggcgac gagaagaacc cactgtataa
     4021 gtactatgag gagacaggca actacctgac aaagtatagc aagaaggata atggccccgt
     4081 gatcaagaag atcaagtact atggcaacaa gctgaatgcc cacctggaca tcaccgacga
     4141 ttaccctaac tctcgcaata aggtggtgaa gctgagcctg aagccatacc ggttcgacgt
     4201 gtacctggac aacggcgtgt ataagtttgt gacagtgaag aatctggatg tgatcaagaa
     4261 ggagaactac tatgaggtga acagcaagtg ctacgaggag gccaagaagc tgaagaagat
     4321 cagcaaccag gccgagttca tcgcctcttt ttacaacaat gacctgatca agatcaatgg
     4381 cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg aacagaatcg aagtgaatat
     4441 gatcgacatc acctacaggg agtatctgga gaacatgaat gataagaggc cccctcgcat
     4501 catcaagacc atcgcctcta agacacagag catcaagaag tacagcacag acatcctggg
     4561 gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt atcaagaaag gctctggtgg
     4621 ttctaagcga cctgccgcca caaagaaggc tggacaggct aagaagaaga aagattacaa
     4681 agacgatgac gataagggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga
     4741 tgtcgaagag aatcctggac cgatggccaa gcctttgtct caagaagaat ccaccctcat
     4801 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4861 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     4921 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     4981 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5041 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5101 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5161 agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     5221 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5281 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5341 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5401 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5461 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5521 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5581 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5641 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5701 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5761 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5821 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5881 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5941 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6001 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6061 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6121 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6181 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6241 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6301 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6361 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6421 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6481 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6541 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6601 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6661 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6721 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6781 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6841 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6901 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6961 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7021 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7081 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7141 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7201 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7261 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7321 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7381 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7441 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7501 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7561 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7621 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7681 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7741 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7801 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7861 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7921 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7981 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8101 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8161 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8221 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8281 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8341 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8401 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8461 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8521 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8581 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8641 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8701 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8761 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8821 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8881 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8941 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9001 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9061 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9121 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9181 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9241 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9301 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9361 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9421 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9481 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9541 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9601 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     9661 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9721 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     9781 aacaagg
//
