LOCUS       sequence-267735-       10765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding miniABEMax (evolved Tada monomer with V82G) at the 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10765)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 V82G ABEMax Strategy 3
FEATURES             Location/Qualifiers
     source          1..10765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     770..789
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        770..788
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5006..5026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5042..5062
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5657..5704
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5714..5770
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6263..6280)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6269..6493
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6539..6967
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6626..6645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6836..6857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6976..6996)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6981..7310
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7161..7296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7223..7242
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7377..8171
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7431..7450)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8041..8060
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8345..8466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8382..8401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8436..8455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8515..8531)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8515..8531)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8528..8550)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8539..8555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8563..8569,8570..8587,8588..8593))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8608..8629
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8746..8763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8917..9505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8997..9016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9676..10467,10468..10536))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10299..10318
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10537..10641)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10716..10735)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
       61 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      121 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      181 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      241 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      301 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      361 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      421 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      481 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      541 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      601 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      661 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      721 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      781 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgat
      841 aggcaaggca accgctaagt atttttttta cagcaacatc atgaacttct ttaagaccga
      901 gattaccctg gccaacggtg aaatccgtaa acgcccgctg attgaaacca acggtgaaac
      961 cggcgaaatc gtgtgggaca agggccgtga ttttgcgacc gtgcgcaaag tgctgtccat
     1021 gccgcaggtg aacattgtga agaaaaccga ggtgcagacc ggcggtttca gcaaggaaag
     1081 cattctgccg aaacgtaaca gcgacaagct gatcgcccgc aagaaagatt gggacccgaa
     1141 gaaatacggc ggtttcgata gcccgaccgt ggcttatagc gtgctggtgg tggccaaagt
     1201 ggagaagggt aaaagcaaga aactgaagag cgtgaaagaa ctgctgggca tcaccattat
     1261 ggagcgtagc agcttcgaaa agaacccgat cgactttctg gaggcgaagg gttacaagga
     1321 agtgaagaaa gatctgatca ttaagctgcc gaaatacagc ctgtttgagc tggaaaatgg
     1381 tcgtaaacgt atgctggcca gcgccggcga gctgcaaaag ggcaacgaac tggcgctgcc
     1441 gagcaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga aaggtagccc
     1501 ggaggacaac gaacagaagc agctgtttgt ggagcagcac aaacactatc tggatgagat
     1561 cattgaacag atcagcgagt tcagcaagcg tgtgattctg gcggacgcta acctggataa
     1621 agtgctgagc gcctacaaca aacaccgtga caagccgatc cgcgagcagg cggaaaacat
     1681 cattcacctg ttcaccctga ccaacctggg tgcgccggcc gcgttcaagt actttgacac
     1741 caccattgat cgtaaacgct ataccagcac caaggaggtg ctggatgcta ccctgatcca
     1801 ccagagcatt accggcctgt acgaaacccg tattgatctg agccagctgg gcggtgacgg
     1861 cggtagcggc ggtagcggcg gtagcggcgg tagcggcggt agcggcggta gcggcggtga
     1921 caagaaatat agcatcggtc tggcgattgg caccaacagc gtgggttggg ctgtgatcac
     1981 cgatgagtac aaggtgccga gcaagaagtt caaggtgctg ggcaacaccg accgccacag
     2041 catcaagaaa aacctgattg gtgccctgct gtttgatagc ggtgaaaccg ccgaggccac
     2101 ccgtctgaag cgcaccgccc gtcgccgtta cacccgtcgt aaaaaccgta tctgctacct
     2161 gcaagagatt ttcagcaacg aaatggccaa agtggacgat agcttctttc accgcctgga
     2221 ggagagcttc ctggtggagg aagacaagaa acacgagcgt cacccgatct ttggcaacat
     2281 tgtggatgag gtggcttacc acgaaaagta tccgaccatc taccacctgc gtaagaaact
     2341 ggtggatagc accgacaagg ccgatctgcg cctgatctat ctggctctgg cccacatgat
     2401 caagttccgt ggtcactttc tgatcgaggg cgacctgaac ccggataaca gcgatgtgga
     2461 caagctgttc atccagctgg tgcagaccta caaccagctg tttgaggaaa acccgattaa
     2521 cgccagcggt gtggacgcga aggctatcct gagcgcgcgt ctgagcaaaa gccgccgtct
     2581 ggagaacctg atcgcgcagc tgccgggtga aaagaaaaac ggtctgtttg gcaacctgat
     2641 tgctctgagc ctgggcctga ccccgaactt caaaagcaac tttgacctgg cggaggatgc
     2701 taagctgcaa ctgagcaagg atacctatga cgatgacctg gacaacctgc tggcgcagat
     2761 cggcgaccag tacgctgatc tgttcctggc tgccaagaac ctgagcgatg cgatcctgct
     2821 gagcgacatt ctgcgtgtga acaccgagat taccaaggcc ccgctgagcg cgagcatgat
     2881 caaacgctat gatgaacacc accaggacct gaccctgctg aaagctctgg tgcgtcagca
     2941 gctgccggag aagtacaaag aaatcttctt tgaccagagc aagaacggtt acgccggcta
     3001 tattgatggc ggtgcgagcc aggaggagtt ctacaagttt atcaaaccga ttctggagaa
     3061 aatggacggc accgaggaac tgctggtgaa gctgaaccgt gaagatctgc tgcgtaaaca
     3121 gcgcaccttc gacaacggta gcatcccgca ccagattcac ctgggtgagc tgcacgccat
     3181 cctgcgccgt caggaagatt tctacccgtt tctgaaggac aaccgtgaga agatcgaaaa
     3241 aattctgacc ttccgcattc cgtactatgt gggtccgctg gctcgtggca acagccgctt
     3301 tgcctggatg acccgcaaga gcgaggaaac catcaccccg tggaacttcg aggaagtggt
     3361 ggacaaaggc gccagcgcgc agagcttcat tgagcgtatg accaactttg ataagaacct
     3421 gccgaacgaa aaagtgctgc cgaagcacag cctgctgtac gagtatttca ccgtgtacaa
     3481 cgaactgacc aaggtgaaat atgtgaccga gggtatgcgc aagccggcct tcctgagcgg
     3541 cgaacagaag aaagcgatcg tggacctgct gtttaaaacc aaccgtaagg tgaccgtgaa
     3601 gcagctgaaa gaggattatt tcaagaaaat tgaatgcttt gacagcgtgg agatcagcgg
     3661 tgtggaagat cgttttaacg cgagcctggg cacctaccac gacctgctga aaatcattaa
     3721 ggataaagac ttcctggata acgaggaaaa cgaggacatc ctggaagata ttgtgctgac
     3781 cctgaccctg tttgaggacc gtgaaatgat cgaggaacgc ctgaagacct atgcccacct
     3841 gttcgatgac aaagtgatga aacagctgaa gcgccgtcgc tacaccggtt ggggccgtct
     3901 gagccgcaaa ctgatcaacg gtattcgcga taaacagagc ggcaagacca tcctggattt
     3961 tctgaagagc gacggtttcg cgaaccgtaa ctttatgcag ctgattcacg atgacagcct
     4021 gaccttcaaa gaggacatcc agaaggcgca ggtgagcggt cagggcgata gcctgcacga
     4081 acatattgct aacctggccg gtagcccggc catcaagaag ggcatcctgc aaaccgtgaa
     4141 ggtggtggac gagctggtga aagtgatggg tcgtcacaag ccggaaaaca tcgtgattga
     4201 gatggcccgc gaaaaccaga ccacccagaa aggccagaag aacagccgtg agcgcatgaa
     4261 gcgtatcgag gaaggcatca aggaactggg cagccagatc ctgaaggagc acccggtgga
     4321 aaacacccag ctgcaaaacg agaaactgta cctgtactac ctgcaaaacg gtcgtgacat
     4381 gtatgtggat caggaactgg acattaaccg cctgagcgat tatgacgtgg atgcgatcgt
     4441 gccgcagagc ttcctgaagg atgacagcat tgacaacaaa gtgctgaccc gtagcgataa
     4501 aaaccgcggc aagagcgaca acgtgccgag cgaggaagtg gtgaagaaaa tgaaaaacta
     4561 ctggcgtcag ctgctgaacg ccaaactgat cacccagcgt aagtttgaca acctgaccaa
     4621 agctgagcgc ggcggtctga gcgaactgga taaagccggt ttcattaagc gtcagctggt
     4681 ggaaacccgc cagatcacca agcacgtggc gcagattctg gacagccgca tgaacaccaa
     4741 gtatgacgag aacgataaac tgatccgtga agtgaaagtg atcaccctga agagcaaact
     4801 ggtgagcgat tttcgtaaag acttccagtt ttacaaggtg cgcgagatca acaactacca
     4861 ccacgctcac gacgcctatc tgaacgcggt ggtgggcacc gccctgatca agaagtaccc
     4921 gaagctggag agcgagttcg tgtacggcga ctacaaggtg tatgatgtgc gtaagatgat
     4981 cgccaaaagc gagcaggaaa ttggcccgaa gaaaaagcgt aaagtgggta gcagcggcag
     5041 cccgaaaaag aaacgcaaag tgggtagcga cgctctggat gacttcgacc tggatatgct
     5101 gggcagcgac gccctggatg actttggcgg tggcagctct gaggtggagt tttcccacga
     5161 gtactggatg agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc
     5221 tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat
     5281 cggcctgcac gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt
     5341 catgcagaac tacagactga ttgacgccac cctgtacggc acattcgagc cttgcgtgat
     5401 gtgcgccggc gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc
     5461 aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg
     5521 cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt
     5581 tcggatgcct agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgacggcgg
     5641 cgatagcggt ggtagcaagc gtccggcggc taccaagaaa gcgggccagg ctaagaaaaa
     5701 gaaaggtagc ggcgctacca acttcagcct gctgaagcag gcgggcgacg tggaggaaaa
     5761 tccgggcccg atggctaaac cgctgagcca ggaggaaagc accctgatcg agcgcgctac
     5821 cgccaccatt aacagcatcc cgattagcga agactatagc gtggccagcg ccgcgctgag
     5881 cagcgatggt cgtattttca ccggcgtgaa cgtgtaccac tttaccggtg gcccgtgcgc
     5941 cgagctggtg gtgctgggca ccgccgccgc cgccgccgcc ggcaacctga cctgcatcgt
     6001 ggcgattggt aacgaaaatc gtggcatcct gagcccgtgc ggccgttgcc gccaggtgct
     6061 gctggacctg catccgggca tcaaggcgat tgtgaaggat agcgacggcc aaccgaccgc
     6121 cgtgggtatt cgtgaactgc tgccatccgg ctatgtttgg gagggctaag gatccactag
     6181 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
     6241 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6301 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6361 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6421 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6481 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6721 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6841 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6901 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6961 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     7021 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     7081 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     7141 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     7201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     7261 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7321 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     7381 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7441 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7501 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7561 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7621 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7681 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7741 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7801 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7861 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7921 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7981 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     8041 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     8101 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8161 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8221 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8281 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8341 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8461 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     8521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8641 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8701 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     9361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    10021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    10081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    10681 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
    10741 ttaagccagt atctgctccc tgctt
//
