LOCUS       sequence-267737-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (D127 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 D127 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..31)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        203..582
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        583..786
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     737..757
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     831..850
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        831..849
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1256..1276
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1292..1312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4739..4786
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4787..4810
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4820..4876
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4877..5275
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5346..5363)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5352..5576
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5622..6050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5709..5728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5919..5940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6059..6079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6064..6393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6244..6379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6306..6325
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6460..7254
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6514..6533)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7124..7143
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7428..7549
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7465..7484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7519..7538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7598..7614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7598..7614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7611..7633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7622..7638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7646..7652,7653..7670,7671..7676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7691..7712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7829..7846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8000..8588)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8080..8099)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8759..9550,9551..9619))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9382..9401
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9620..9724)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
       61 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
      121 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
      181 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      241 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      301 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      361 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      421 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      481 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      541 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      601 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      661 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      721 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      781 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      841 cactataggg agacccaagc tggctagcgc caccatgaag cgaaattaca ttctggggct
      901 ggccattggc attacatcag tgggctatgg catcattgac tacgagacaa gggacgtgat
      961 cgacgccggc gtgagactgt tcaaggaggc caacgtggag aacaatgagg gccggagatc
     1021 caagagggga gcaaggcgcc tgaagcggag aaggcgccac agaatccaga gagtgaagaa
     1081 gctgctgttc gattacaacc tgctgaccga ccactccgag ctgtctggca tcaatcctta
     1141 tgaggccaga gtgaagggcc tgtcccagaa gctgtctgag gaggagttta gcgccgccct
     1201 gctgcacctg gcaaagagga gaggcgtgca caacgtgaat gaggtggagg aggacccgaa
     1261 gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag aagcgcaaag tgggcagcga
     1321 cgcgctggac gatttcgatc tggacatgct gggcagcgat gcgctggatg atttcggcgg
     1381 tggcagctct gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc
     1441 caagagggca cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag
     1501 agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga
     1561 aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac
     1621 cctgtacggc acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat
     1681 cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga
     1741 cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
     1801 tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca
     1861 gaagaaggcc cagagctcca ccgacggcgg tagcggcggt agcggtggta gcggcggcag
     1921 cggtggtagc ggtggcagcg gtggtgacac cggcaacgag ctgtccacaa aggagcagat
     1981 cagccgcaat tccaaggccc tggaggagaa gtatgtggcc gagctgcagc tggagcggct
     2041 gaagaaggat ggcgaggtga ggggctccat caatcgcttc aagacctctg actacgtgaa
     2101 ggaggccaag cagctgctga aggtgcagaa ggcctaccac cagctggatc agtcctttat
     2161 cgatacatat atcgacctgc tggagacaag gcgcacatac tatgagggac caggagaggg
     2221 ctctcccttc ggctggaagg acatcaagga gtggtacgag atgctgatgg gccactgcac
     2281 ctattttcca gaggagctga gaagcgtgaa gtacgcctat aacgccgatc tgtacaacgc
     2341 cctgaatgac ctgaacaacc tggtcatcac cagggatgag aacgagaagc tggagtacta
     2401 tgagaagttc cagatcatcg agaacgtgtt caagcagaag aagaagccta cactgaagca
     2461 gatcgccaag gagatcctgg tgaacgagga ggacatcaag ggctaccgcg tgacctccac
     2521 aggcaagcca gagttcacca atctgaaggt gtatcacgat atcaaggaca tcacagcccg
     2581 gaaggagatc atcgagaacg ccgagctgct ggatcagatc gccaagatcc tgaccatcta
     2641 tcagagctcc gaggacatcc aggaggagct gaccaacctg aatagcgagc tgacacagga
     2701 ggagatcgag cagatcagca atctgaaggg ctacaccggc acacacaacc tgagcctgaa
     2761 ggccatcaat ctgatcctgg atgagctgtg gcacacaaac gacaatcaga tcgccatctt
     2821 taaccggctg aagctggtgc caaagaaggt ggacctgtcc cagcagaagg agatcccaac
     2881 cacactggtg gacgatttca tcctgtctcc cgtggtgaag cggagcttca tccagagcat
     2941 caaagtgatc aacgccatca tcaagaagta cggcctgccc aatgatatca tcatcgagct
     3001 ggccagggag aagaactcca aggacgccca gaagatgatc aatgagatgc agaagaggaa
     3061 ccgccagacc aatgagcgga tcgaggagat catcagaacc acaggcaagg agaacgccaa
     3121 gtacctgatc gagaagatca agctgcacga tatgcaggag ggcaagtgtc tgtattctct
     3181 ggaggccatc cctctggagg acctgctgaa caatccattc aactacgagg tggatcacat
     3241 catcccccgg agcgtgagct tcgacaattc ttttaacaat aaggtgctgg tgaagcagga
     3301 ggagaacagc aagaagggca ataggacccc tttccagtac ctgtctagct ccgattctaa
     3361 gatcagctac gagacattca agaagcacat cctgaatctg gccaagggca agggccgcat
     3421 cagcaagacc aagaaggagt acctgctgga ggagcgggac atcaacagat tctccgtgca
     3481 gaaggacttc atcaaccgga atctggtgga caccagatac gccacacgcg gcctgatgaa
     3541 tctgctgcgg tcttatttca gagtgaacaa tctggatgtg aaggtgaaga gcatcaacgg
     3601 cggcttcacc tcctttctgc ggagaaagtg gaagtttaag aaggagcgca acaagggcta
     3661 taagcaccac gccgaggatg ccctgatcat cgccaatgcc gacttcatct ttaaggagtg
     3721 gaagaagctg gacaaggcca agaaagtgat ggagaaccag atgttcgagg agaagcaggc
     3781 cgagagcatg cccgagatcg agacagagca ggagtacaag gagattttca tcacacctca
     3841 ccagatcaag cacatcaagg acttcaagga ctacaagtat tctcacaggg tggataagaa
     3901 gcccaaccgc gagctgatca atgacaccct gtatagcaca cggaaggacg ataagggcaa
     3961 taccctgatc gtgaacaatc tgaacggcct gtacgacaag gataatgaca agctgaagaa
     4021 gctgatcaac aagtctcccg agaagctgct gatgtaccac cacgatcctc agacatatca
     4081 gaagctgaag ctgatcatgg agcagtacgg cgacgagaag aacccactgt ataagtacta
     4141 tgaggagaca ggcaactacc tgacaaagta tagcaagaag gataatggcc ccgtgatcaa
     4201 gaagatcaag tactatggca acaagctgaa tgcccacctg gacatcaccg acgattaccc
     4261 taactctcgc aataaggtgg tgaagctgag cctgaagcca taccggttcg acgtgtacct
     4321 ggacaacggc gtgtataagt ttgtgacagt gaagaatctg gatgtgatca agaaggagaa
     4381 ctactatgag gtgaacagca agtgctacga ggaggccaag aagctgaaga agatcagcaa
     4441 ccaggccgag ttcatcgcct ctttttacaa caatgacctg atcaagatca atggcgagct
     4501 gtatagagtg atcggcgtga acaatgatct gctgaacaga atcgaagtga atatgatcga
     4561 catcacctac agggagtatc tggagaacat gaatgataag aggccccctc gcatcatcaa
     4621 gaccatcgcc tctaagacac agagcatcaa gaagtacagc acagacatcc tggggaacct
     4681 gtatgaagtc aagagcaaga aacatcctca gattatcaag aaaggctctg gtggttctaa
     4741 gcgacctgcc gccacaaaga aggctggaca ggctaagaag aagaaagatt acaaagacga
     4801 tgacgataag ggatccggcg caacaaactt ctctctgctg aaacaagccg gagatgtcga
     4861 agagaatcct ggaccgatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     4921 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     4981 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     5041 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     5101 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     5161 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     5221 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagaatt
     5281 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     5341 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5401 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5461 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5521 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5581 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5641 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5701 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5761 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5821 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5881 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5941 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6001 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6061 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6121 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6181 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6301 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6361 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6421 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     6481 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6541 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6601 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6661 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6721 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6781 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6841 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6901 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6961 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7021 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7081 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7141 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7201 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7261 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7321 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7381 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7441 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7501 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7561 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8581 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8641 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8701 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8761 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8821 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8881 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8941 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9001 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9061 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9541 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9601 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9661 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9721 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     9781 cctatgg
//
