LOCUS       sequence-267749-        3555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For making TNKS-2-M1054V RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Williams C
  TITLE     Williams Lab Plasmids 2020
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEMHE-TNKS-2-M1054V
FEATURES             Location/Qualifiers
     source          1..3555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1612..1623
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 accggtgcca ccatggacta caaagacgat gacgacaagc ttgcggccgc gtcgggtcgc
       61 cgctgcgccg gcgggggagc ggcctgcgcg agcgccgcgg ccgaggccgt ggagccggcc
      121 gcccgagagc tgttcgaggc gtgccgcaac ggggacgtgg aacgagtcaa gaggctggtg
      181 acgcctgaga aggtgaacag ccgcgacacg gcgggcagga aatccacccc gctgcacttc
      241 gccgcaggtt ttgggcggaa agacgtagtt gaatatttgc ttcagaatgg tgcaaatgtc
      301 caagcacgtg atgatggggg ccttattcct cttcataatg catgctcttt tggtcatgct
      361 gaagtagtca atctcctttt gcgacatggt gcagacccca atgctcgaga taattggaat
      421 tatactcctc tccatgaagc tgcaattaaa ggaaagattg atgtttgcat tgtgctgtta
      481 cagcatggag ctgagccaac catccgaaat acagatggaa ggacagcatt ggatttagca
      541 gatccatctg ccaaagcagt gcttactggt gaatataaga aagatgaact cttagaaagt
      601 gccaggagtg gcaatgaaga aaaaatgatg gctctactca caccattaaa tgtcaactgc
      661 cacgcaagtg atggcagaaa gtcaactcca ttacatttgg cagcaggata taacagagta
      721 aagattgtac agctgttact gcaacatgga gctgatgtcc atgctaaaga taaaggtgat
      781 ctggtaccat tacacaatgc ctgttcttat ggtcattatg aagtaactga acttttggtc
      841 aagcatggtg cctgtgtaaa tgcaatggac ttgtggcaat tcactcctct tcatgaggca
      901 gcttctaaga acagggttga agtatgttct cttctcttaa gttatggtgc agacccaaca
      961 ctgctcaatt gtcacaataa aagtgctata gacttggctc ccacaccaca gttaaaagaa
     1021 agattagcat atgaatttaa aggccactcg ttgctgcaag ctgcacgaga agctgatgtt
     1081 actcgaatca aaaaacatct ctctctggaa atggtgaatt tcaagcatcc tcaaacacat
     1141 gaaacagcat tgcattgtgc tgctgcatct ccatatccca aaagaaagca aatatgtgaa
     1201 ctgttgctaa gaaaaggagc aaacatcaat gaaaagacta aagaattctt gactcctctg
     1261 cacgtggcat ctgagaaagc tcataatgat gttgttgaag tagtggtgaa acatgaagca
     1321 aaggttaatg ctctggataa tcttggtcag acttctctac acagagctgc atattgtggt
     1381 catctacaaa cctgccgcct actcctgagc tatgggtgtg atcctaacat tatatccctt
     1441 cagggcttta ctgctttaca gatgggaaat gaaaatgtac agcaactcct ccaagagggt
     1501 atctcattag gtaattcaga ggcagacaga caattgctgg aagctgcaaa ggctggagat
     1561 gtcgaaactg taaaaaaact gtgtactgtt cagagtgtca actgcagaga cattgaaggg
     1621 cgtcagtcta caccacttca ttttgcagct gggtataaca gagtgtccgt ggtggaatat
     1681 ctgctacagc atggagctga tgtgcatgct aaagataaag gaggccttgt acctttgcac
     1741 aatgcatgtt cttatggaca ttatgaagtt gcagaacttc ttgttaaaca tggagcagta
     1801 gttaatgtag ctgatttatg gaaatttaca cctttacatg aagcagcagc aaaaggaaaa
     1861 tatgaaattt gcaaacttct gctccagcat ggtgcagacc ctacaaaaaa aaacagggat
     1921 ggaaatactc ctttggatct tgttaaagat ggagatacag atattcaaga tctgcttagg
     1981 ggagatgcag ctttgctaga tgctgccaag aagggttgtt tagccagagt gaagaagttg
     2041 tcttctcctg ataatgtaaa ttgccgcgat acccaaggca gacattcaac acctttacat
     2101 ttagcagctg gttataataa tttagaagtt gcagagtatt tgttacaaca cggagctgat
     2161 gtgaatgccc aagacaaagg aggacttatt cctttacata atgcagcatc ttacgggcat
     2221 gtagatgtag cagctctact aataaagtat aatgcatgtg tcaatgccac ggacaaatgg
     2281 gctttcacac ctttgcacga agcagcccaa aagggacgaa cacagctttg tgctttgttg
     2341 ctagcccatg gagctgaccc gactcttaaa aatcaggaag gacaaacacc tttagattta
     2401 gtttcagcgg atgatgtcag cgctcttctg acagcagcca tgcccccatc tgctctgccc
     2461 tcttgttaca agcctcaagt gctcaatggt gtgagaagcc caggagccac tgcagatgct
     2521 ctctcttcag gtccatctag cccatcaagc ctttctgcag ccagcagtct tgacaactta
     2581 tctgggagtt tttcagaact gtcttcagta gttagttcaa gtggaacaga gggtgcttcc
     2641 agtttggaga aaaaggaggt tccaggagta gattttagca taactcaatt cgtaaggaat
     2701 cttggacttg agcacctaat ggatatattt gagagagaac agatcacttt ggatgtatta
     2761 gttgagatgg ggcacaagga gctgaaggag attggaatca atgcttatgg acataggcac
     2821 aaactaatta aaggagtcga gagacttatc tccggacaac aaggtcttaa cccatattta
     2881 actttgaaca cctctggtag tggaacaatt cttatagatc tgtctcctga tgataaagag
     2941 tttcagtctg tggaggaaga gatgcaaagt acagttcgag agcacagaga tggaggtcat
     3001 gcaggtggaa tcttcaacag atacaatatt ctcaagattc agaaggtttg taacaagaaa
     3061 ctatgggaaa gatacactca ccggagaaaa gaagtttctg aagaaaacca caaccatgcc
     3121 aatgaacgaa tgctatttca tgggtctcct tttgtgaatg caattatcca caaaggcttt
     3181 gatgaaaggc atgcgtacat aggtggtatg tttggagctg gcatttattt tgctgaaaac
     3241 tcttccaaaa gcaatcaata tgtatatgga attggaggag gtactgggtg tccagttcac
     3301 aaagacagat cttgttacat ttgccacagg cagctgctct tttgccgggt aaccttggga
     3361 aagtctttcc tgcagttcag tgcaatgaaa atggcacatt ctcctccagg tcatcactca
     3421 gtcactggta ggcccagtgt aaatggccta gcattagctg aatatgttat ttacagagga
     3481 gaacaggctt atcctgagta tttaattact taccagatta tgaggcctga aggtatggtc
     3541 gatggataat ctaga
//
