LOCUS       sequence-267750-        9145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, copper-inducible expression of the reporter fluorophor
            mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning 
            of GOI downstream of the PpetE:BCD2 expression module..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
  TITLE     High density cultivation for efficient sesquiterpenoid biosynthesis 
            in Synechocystis sp. PCC 6803.
  JOURNAL   Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w.
  PUBMED    32246065
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-3"; volume: "10(1):5932. doi"; pages: " 
            10.1038/s41598-020-62681-w"
COMMENT     Sequence Label: pSHDY*in_PpetE:BCD2:mVenus
FEATURES             Location/Qualifiers
     source          1..9145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(160..975)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(160..975)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     883..902
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(976..1067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1093..1110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1154..1945)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2151..2172
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2175..2246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2262..2289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2803..2811
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      3557..3628
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3644..3671
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3678..3698
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(3899..4750)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4737..5576)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(6087..7058)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            8297..8384
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      8726..9120
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 ggtaccgagt cgatgtcgac taacgaccct gccctgaacc gacgacaagc tgacgaccgg
       61 gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      121 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
      181 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      241 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      301 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      361 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      421 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      481 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
      541 gcggtcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      601 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      661 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      721 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      781 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      841 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      901 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
      961 ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gcatgcagcg
     1081 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactct cgagagcggc
     1141 cgaagcttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     1201 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     1261 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     1321 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     1381 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     1441 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     1501 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     1561 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     1621 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc ataacaatgg
     1681 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     1741 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     1801 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     1861 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     1921 cttcggcgat caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct
     1981 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     2041 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
     2101 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag gaattcgcgg
     2161 ccgcttctag agatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2221 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2281 ctttctgcgt ttttacccta cacttaaacc ccatctcaat acttcccatc aaatccaacc
     2341 taactcatac attccaaact aatctaactt cttccatcat taaccacacc tctctatcat
     2401 cacctattcc cctctatctc cctcaaattc tttcacatat taaaaactta ctcttcctcc
     2461 tttcctcctt aaaatactaa ataactctta aaatagaagg gatagcaagc taatttttat
     2521 gacggcgatc gccaaaaaca aagaaaattc agcaattacc gtgggtagca aaaaatcccc
     2581 atctaaagtt cagtaaatat agctagaaca accaagcatt ttcggcaaag tactattcag
     2641 atagaacgag aaatgagctt gttctatccg cccggggctg aggctgtata atctacgacg
     2701 ggctgtcaaa cattgtgata ccatgggcag aagggcccaa gttcacttaa aaaggagatc
     2761 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
     2821 gctagcaaag gagaggaact gtttaccggt gtggtaccca ttttagtaga attggatggt
     2881 gatgtgaacg gccacaagtt cagcgtttcc ggggaaggcg aaggggatgc aacctatgga
     2941 aagctaactt tgaaactcat ttgcactacc ggtaaactcc ccgtcccctg gcctacgtta
     3001 gtcacgaccc tcggttatgg tctgcaatgt tttgcccgtt atcccgatca tatgaaacaa
     3061 cacgatttct ttaaatctgc catgccagag ggatatgttc aagaacggac aattttcttt
     3121 aaggatgatg gtaattataa aacccgtgca gaagtcaaat ttgaaggtga tacactcgtg
     3181 aatcgcatcg aactaaaagg tattgacttt aaggaggatg gaaacattct cggccataag
     3241 ttagaataca attacaacag ccataatgtg tacattactg cggataaaca aaaaaacggt
     3301 atcaaagcca actttaaaat tcgacataac atcgaagatg gtggcgttca gttagcggat
     3361 cactaccaac aaaatactcc cattggagac ggaccagtcc tgctgcctga taaccattat
     3421 ctgtcttatc aatccaaact gtccaaggat ccgaatgaaa agcgggatca catggttctg
     3481 ttggagtttg tgaccgcggc aggtattact ctcggaatgg acgaactata caaataatag
     3541 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3601 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     3661 gcctttctgc gtttatatac tagtagcggc cgctgcagga gcagaagagc atacatctgg
     3721 aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc
     3781 aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga
     3841 aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt ggggggggtc
     3901 agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc
     3961 agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg
     4021 tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc
     4081 cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc
     4141 tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg
     4201 cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt
     4261 actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct
     4321 tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg
     4381 ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga
     4441 cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt
     4501 ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca
     4561 gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt
     4621 catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc
     4681 cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct gaggctgtgc ttgttcttag
     4741 gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc
     4801 accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg
     4861 ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc
     4921 tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag
     4981 ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca
     5041 tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg
     5101 acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc
     5161 agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc
     5221 atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc
     5281 cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc
     5341 gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga
     5401 tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt accggcacca
     5461 ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc
     5521 ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt agccattgat
     5581 tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc ccgccctgcg
     5641 ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga
     5701 acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct tggcgtacag
     5761 cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc atatcagtca
     5821 ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg
     5881 tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc cttgtttgac
     5941 gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag gtagaccgac
     6001 cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta ctcatagtat
     6061 aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc cctgtccatg
     6121 cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc
     6181 agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc
     6241 tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc actgcggccc
     6301 cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc
     6361 ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta
     6421 agctgccgct cgggcagttc gaggctggcc agcctgcggg ccttctcctg ctgccgctgg
     6481 gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt ggcggtcttg
     6541 cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc
     6601 ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct ggccgggtcg
     6661 gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc gcctgcggcg
     6721 tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac cagggcaggc
     6781 tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc
     6841 agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg ggcattcatc
     6901 cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg gttcatcatc
     6961 tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat ggccttgagc
     7021 tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga
     7081 tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca
     7141 gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc cagtgccacc
     7201 cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct acctcccgca
     7261 actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc agccactccg
     7321 ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct tcgtcggcca
     7381 gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg atgcctctgg
     7441 atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc ctgtaagtcg
     7501 atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg
     7561 ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag gcgctcgatg
     7621 ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggccagcccg ctctaatgcc
     7681 cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc
     7741 gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac
     7801 tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag gtggcagtgc
     7861 gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgctc ggcaccggtc
     7921 aggtgctggg cgaactcgga cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc
     7981 agggcaaatt cgacctcctt gaacagccgc ccattggcgc gttcatacag gtcggcagca
     8041 tcccagtagt cggcgggccg ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag
     8101 acttcatcca tgtcgcgggc atacttgcct tcgcgctgga tgtagtcggc cttggccctg
     8161 gccgattggc cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg
     8221 cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc ctcggagagc gcaccgcccg
     8281 aagggtggcc gttaggccag tttctcgaag agaaaccggt aagtgcgccc tcccctacaa
     8341 agtagggtcg ggattgccgc cgctgtgcct ccatgatagc ctacgagaca gcacattaac
     8401 aatggggtgt caagatggtt aaggggagca acaaggcggc ggatcggctg gccaagctcg
     8461 aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc
     8521 aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt ttggccaagg
     8581 tgaacagcag cgagtggccg gaggatcggc tcatggcggc aatggatgcg taccttgaac
     8641 gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa
     8701 tgatcgaccg agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg
     8761 aaaggccgct aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt
     8821 ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag
     8881 cgcagcgaat agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg
     8941 ccccacaagg gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat
     9001 ttttccaaca ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag
     9061 ttattgcagg ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg
     9121 tgacagttag tacgggagtg acggg
//
