LOCUS       sequence-267752-        9335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF5A 378-1027.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
FEATURES             Location/Qualifiers
     source          1..9335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..36
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..697
                     /label=chicken beta-actin promoter
     primer_bind     1638..1660
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1736..2620
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             3077..3202
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          5227..5359
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5342..5361
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5368..5956
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5421..5441)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5839..5850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5983..6000)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5989..6100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6196..6216)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6196..6214)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6235..6251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6357..6376)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6422..6477
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6476..6495)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6838..6854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6862..6878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6886..6892,6893..6910,6911..6916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6931..6952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7011..7206
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7057..7192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7212..7346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7249..7268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7303..7322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7414..7431)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7585..8173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7665..7684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8344..9135,9136..9204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8967..8986
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9205..9309)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagagtcatc ctcatcgtcg acattgatta ttgactagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      601 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      661 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
      721 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      781 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
      841 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
      901 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1021 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1081 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1141 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     1201 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     1261 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1321 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1381 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1441 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1501 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1561 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     1621 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta ggctgctgaa
     1681 gcggtagcat atgctcccag aaggcgcgcc taggaccatg gctagcggag gatccgaaat
     1741 cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta
     1801 cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc
     1861 ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc
     1921 agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca
     1981 cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat
     2041 tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa
     2101 aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt
     2161 tgcccgcgag accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga
     2221 tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt
     2281 cgagatggac cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg
     2341 cttcccaaac gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga
     2401 atacatggac tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg
     2461 cgttctgatc ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc
     2521 tgtggacatc ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag
     2581 cgagatcgcg cgctggctgt ctactctgga gatttccggg ctgtacggtg ctggagcaga
     2641 tctgggtgcc ggagctggcg ccggggctgg agcaggctct cgctttcagt gcagaatctg
     2701 catgcgaaat ttcagcgacc ggtccaacct gtctcgacat attcggaccc acacaggcga
     2761 aaaacctttt gcctgtgaca tctgtgggcg caagttcgcc atttcaagta acctcaattc
     2821 acataccaag atccatacag ggtctcaaaa gccatttcag tgccgcatct gtatgaggaa
     2881 tttcagcaga agcgataacc ttgcacgcca catccgcacg cacactggtg agaagccttt
     2941 cgcatgtgac atttgcggac gcaagtttgc aacatccggc aatctgacca ggcatacgaa
     3001 gattcatctc cggggttccc agctgcccgg gatcgtcgac gcccctgaac agcgggaagg
     3061 cgcaagccaa gtcagcgtct ctgtaacctt cgaggacgtc gcagtattgt tcacacgaga
     3121 cgaatggaag aaactggacc tgagccagcg aagtctctac agggaagtga tgctggagaa
     3181 ttattcaaac ctggcttcta tggcaggcgg cggctctggc ggcggatcag gaggcggcct
     3241 gtacatagag actgagcgcc tggctggaga ggactcagct ctgggagctg agctctgcga
     3301 ggagacccct gtgaatgaca actcatccat tgtggtacgc atcgcacctg aggaaaggca
     3361 gaaatatgag gaagagatcc gccgtctcta caagcagctt gatgacaagg atgatgagat
     3421 caaccagcag agccagctca ttgagaagct gaagcagcag atgctggacc aggaagagct
     3481 gctcgtgtcc actcggggag acaacgagaa ggtccagcgg gagcttagcc acctgcagtc
     3541 cgagaacgat gctgcgaagg acgaggtgaa ggaagtgctg caggccctag aggagctggc
     3601 ggtcaactac gaccagaagt cccaggaggt ggaggagaag agccagcaga accagctgct
     3661 ggtggacgag ctgtcccaga aagtggccac catgctgtcc ctggagtccg agctacagcg
     3721 gctccaggag gtcagtggac accagcgaaa gcggatcgct gaggtgctga atgggctgat
     3781 gagggacctg agtgagttca gtgtcatcgt gggcaacggc gagattaagc tgcccgtgga
     3841 gatcagtggg gccatcgagg aggagttcac ggtggcccgg ctctacatca gcaagatcaa
     3901 gtcggaggtg aagtccgtgg ttaagcgatg tcggcagctg gagaacctcc aggtggagtg
     3961 tcatcgcaag atggaggtga ccggtaggga gctgtcatct tgccaactgc tcatctcaca
     4021 gcatgaggcc aagatccgtt cactcacgga gtacatgcag actgtggagt tgaagaaacg
     4081 gcacctggaa gagtcctacg actccctgag cgatgagctt gccaggctgc aggcgcacga
     4141 aacggtacac gaggtagctc tgaaagacaa ggagccagac acacaggacg cggaggaggt
     4201 gaagaaggcc ctggaactac agatggagaa tcaccgtgag gcccatcacc ggcagctggc
     4261 ccgcctccgg gatgagatta atgagaaaca gaaaaccatt gatgagctga aagacctgaa
     4321 ccagaagctc cagttagagc tggagaagct tcaggccgac tatgagaggc tgaagaatga
     4381 agagaacgag aagagcgcca agctccagga gctgacattt ctgtatgagc gacatgagca
     4441 gtccaagcag gacctcaagg ggctggagga gacagttgcc cgtgaactcc agaccctcca
     4501 caaccttcgc aagctgttcg ttcaagacgt cacgactcga gtcaagaaaa gtgcagaaat
     4561 ggagcccgag gacagtgggg ggattcattc ccaaaagcag aagatctcct ttcttgagaa
     4621 caacctggaa cagcttacaa aggttcacaa acagctggta cgtgacaatg cagatctgcg
     4681 ttgtgagctt cctaaattgg aaaaacgact tcgggctacg gctgagagag ttaaggccct
     4741 ggagggtgca ctgaaggagg ccaaggaggg cgctatgaag gacaagcgta gataccagca
     4801 ggaggtggac cgcatcaaag aagccgtgcg gtacaagagc tccggcaagc ggggccattc
     4861 tgcccagatc gctaagcctg tgaggcctgg ccactatcct gcctcctcac ccaccaaccc
     4921 ctacggcacc cggagccccg agtgtatcag ctacaccaac aacctcttcc agaactacca
     4981 gaacctgcac ctgcaggctg cgcctagctc cacttcagat atgtactttg ccagcagcgg
     5041 agccacatct gttgccccct tggcttccta ccagaaggcc aacatggaca atggaaatgc
     5101 cacagatatc aacgacaaca ggagtgacct gccgtgtggc tatgaggctg aggaccaggc
     5161 caagcttttc cctctccacc aagagacagc agccagctaa gtctagatag ggactcgagg
     5221 ccgcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     5281 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     5341 tttgcctttc tctccacagg tgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     5401 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5461 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5521 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5581 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5641 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5701 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5761 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5821 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5881 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5941 tgggccgcct ccccgcctgg aattcgagct cggtacgatc agcctcgact gtgccttcta
     6001 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6061 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6121 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6181 gcccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
     6241 gctgtttcct gtgtgaaatt gttatccgct aattcactcc tcaggtgcag gctgcctatc
     6301 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatcgatctt
     6361 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     6421 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     6481 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     6541 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6601 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6661 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6721 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6781 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     6841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     7021 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7201 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7261 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7321 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     7381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9301 ggttccgcgc acatttcccc gaaaagtgcc acctg
//
