LOCUS       sequence-267753-        9146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF5B 376-963.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963
FEATURES             Location/Qualifiers
     source          1..9146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    25..36
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        40..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..697
                     /label=chicken beta-actin promoter
     primer_bind     1638..1660
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1736..2620
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             3077..3202
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          5038..5170
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5153..5172
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5179..5767
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5232..5252)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5650..5661)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5794..5811)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5800..5911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6007..6027)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6007..6025)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6046..6062)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6168..6187)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6233..6288
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6287..6306)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6649..6665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6673..6689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6697..6703,6704..6721,6722..6727))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6742..6763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6822..7017
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6868..7003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7023..7157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7060..7079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7114..7133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7225..7242)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7396..7984)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7476..7495)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8155..8946,8947..9015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8778..8797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9016..9120)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagagtcatc ctcatcgtcg acattgatta ttgactagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      601 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      661 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
      721 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
      781 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
      841 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
      901 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      961 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1021 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1081 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1141 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     1201 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     1261 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1321 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1381 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1441 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1501 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1561 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     1621 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta ggctgctgaa
     1681 gcggtagcat atgctcccag aaggcgcgcc taggaccatg gctagcggag gatccgaaat
     1741 cggtactggc tttccattcg acccccatta tgtggaagtc ctgggcgagc gcatgcacta
     1801 cgtcgatgtt ggtccgcgcg atggcacccc tgtgctgttc ctgcacggta acccgacctc
     1861 ctcctacgtg tggcgcaaca tcatcccgca tgttgcaccg acccatcgct gcattgctcc
     1921 agacctgatc ggtatgggca aatccgacaa accagacctg ggttatttct tcgacgacca
     1981 cgtccgcttc atggatgcct tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat
     2041 tcacgactgg ggctccgctc tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa
     2101 aggtattgca tttatggagt tcatccgccc tatcccgacc tgggacgaat ggccagaatt
     2161 tgcccgcgag accttccagg ccttccgcac caccgacgtc ggccgcaagc tgatcatcga
     2221 tcagaacgtt tttatcgagg gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt
     2281 cgagatggac cattaccgcg agccgttcct gaatcctgtt gaccgcgagc cactgtggcg
     2341 cttcccaaac gagctgccaa tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga
     2401 atacatggac tggctgcacc agtcccctgt cccgaagctg ctgttctggg gcaccccagg
     2461 cgttctgatc ccaccggccg aagccgctcg cctggccaaa agcctgccta actgcaaggc
     2521 tgtggacatc ggcccgggtc tgaatctgct gcaagaagac aacccggacc tgatcggcag
     2581 cgagatcgcg cgctggctgt ctactctgga gatttccggg ctgtacggtg ctggagcaga
     2641 tctgggtgcc ggagctggcg ccggggctgg agcaggctct cgctttcagt gcagaatctg
     2701 catgcgaaat ttcagcgacc ggtccaacct gtctcgacat attcggaccc acacaggcga
     2761 aaaacctttt gcctgtgaca tctgtgggcg caagttcgcc atttcaagta acctcaattc
     2821 acataccaag atccatacag ggtctcaaaa gccatttcag tgccgcatct gtatgaggaa
     2881 tttcagcaga agcgataacc ttgcacgcca catccgcacg cacactggtg agaagccttt
     2941 cgcatgtgac atttgcggac gcaagtttgc aacatccggc aatctgacca ggcatacgaa
     3001 gattcatctc cggggttccc agctgcccgg gatcgtcgac gcccctgaac agcgggaagg
     3061 cgcaagccaa gtcagcgtct ctgtaacctt cgaggacgtc gcagtattgt tcacacgaga
     3121 cgaatggaag aaactggacc tgagccagcg aagtctctac agggaagtga tgctggagaa
     3181 ttattcaaac ctggcttcta tggcaggcgg cggctctggc ggcggatcag gaggcggcct
     3241 gtacattgat gagcagtttg acaaagagaa agctaatttg gaagccttca cagcggataa
     3301 agatattgct attaccagtg ataaaccagc tgctgcagtc ggaatggctg gtagttttac
     3361 cgatgctgaa agaagaaagt gtgaagaaga acttgctaaa ttgtataaac aacttgatga
     3421 caaggatgaa gagattaacc aacaaagcca attggtagag aaattgaaga cacaaatgct
     3481 ggatcaggaa gagcttctgg catcaaccag aagggatcaa gataatatgc aagctgaact
     3541 gaatcgcctc caagcagaaa atgatgcttc taaagaagaa gtcaaagaag ttttacaggc
     3601 cttagaggaa ctggctgtta attatgatca gaagtctcag gaagttgaag acaaaacaaa
     3661 ggaatatgaa ttgcttagtg atgaattgaa tcaaaaatct gcaactttag caagtattga
     3721 tgctgagctt cagaagctga aggaaatgac caaccaccag aagaaacgag cagctgaaat
     3781 gatggcatca ttattaaaag accttgcaga aataggaatt gctgtgggga ataacgatgt
     3841 gaagcaacca gaaggaactg gtatgataga tgaagagttt actgttgcaa gactctacat
     3901 tagcaaaatg aaatcagaag taaagaccat ggtgaaacgc tgcaaacagc tagaaagcac
     3961 acagactgag agcaacaaaa aaatggaaga aaatgagaaa gagttagcag catgccagct
     4021 tcggatctcc caacatgaag ccaaaatcaa gtcactgact gagtaccttc agaatgtaga
     4081 acaaaagaag aggcagctgg aggagtctgt tgattccctt ggtgaggagc tagtccaact
     4141 ccgagcacaa gagaaagtcc atgaaatgga aaaagagcac ttgaacaagg ttcagactgc
     4201 aaatgaagtc aagcaagctg ttgagcagca gatccagagt cacagagaaa cccaccaaaa
     4261 acaaatcagt agtttgcgag atgaagtgga ggcaaaggaa aagctaatca ctgacctcca
     4321 agaccaaaac cagaagatgg tgttggagca ggaacggcta agggtggagc atgagaggct
     4381 gaaggctaca gaccaagaga agagcaggaa gctgcacgag ctcacggtta tgcaagacag
     4441 acgagaacaa gcaagacaag acttgaaggg tttggaggag accgtggcaa aagaacttca
     4501 gactttacac aacctgcgta agctctttgt tcaggacttg gctaccaggg tgaaaaagag
     4561 cgccgaggtc gactctgacg acactggcgg cagtgctgca cagaagcaga aaatctcctt
     4621 ccttgaaaac aaccttgaac agctcaccaa agtgcacaag cagttggtac gcgataatgc
     4681 agatcttcgc tgtgagcttc ctaagttaga gaaacggctt agagctactg cagaaagagt
     4741 gaaagctttg gagtcagccc tgaaagaagc caaagaaaat gcatctcgag accgtaaacg
     4801 ctatcagcaa gaagtagacc ggataaagga agcagtcagg tcaaagaaca tggccagaag
     4861 gggacattct gcccagattg caaagccgat ccgtcctgga cagcatccag cggcctcgcc
     4921 aactcacccg ggcacagttc gtggaggagg ctcatttgtt cagaacaacc agccagtggg
     4981 gcttcgtggt ggtggaggca agcagtcgta agtctagata gggactcgag gccgcaggta
     5041 agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag
     5101 agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt
     5161 ctctccacag gtgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     5221 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     5281 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     5341 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     5401 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5461 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5521 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5581 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5641 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5701 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5761 tccccgcctg gaattcgagc tcggtacgat cagcctcgac tgtgccttct agttgccagc
     5821 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5881 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5941 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcccagctt
     6001 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     6061 tgtgtgaaat tgttatccgc taattcactc ctcaggtgca ggctgcctat cagaaggtgg
     6121 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcgatct ttttccctct
     6181 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
     6241 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
     6301 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
     6361 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
     6421 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
     6481 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
     6541 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
     6601 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
     6661 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6721 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6781 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca
     6841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7021 ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7081 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7141 cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9121 cacatttccc cgaaaagtgc cacctg
//
