LOCUS       sequence-267772-        8424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human progesterone receptor-B in
            pCMV5 with 5 restriction sites deleted in polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: p5M-hPR-B
FEATURES             Location/Qualifiers
     source          1..8424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     RBS             4380..4391
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     polyA_signal    4448..5070
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4647..4666)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5094..5114)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5099..5428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5279..5414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5466..5485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5467..5485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5499..5515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5499..5515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5512..5534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5523..5539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5547..5553,5554..5571,5572..5577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5592..5613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5730..5747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5901..6489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5981..6000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6660..7451,7452..7520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7283..7302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7521..7625)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7652..8107
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7739..7758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7949..7970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8233..8255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8247..8264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8248..8264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattccac catgactgag ctgaaggcaa agggtccccg ggctccccac gtggcgggcg
      661 gcccgccctc ccccgaggtc ggatccccac tgctgtgtcg cccagccgca ggtccgttcc
      721 cggggagcca gacctcggac accttgcctg aagtttcggc catacctatc tccctggacg
      781 ggctactctt ccctcggccc tgccagggac aggacccctc cgacgaaaag acgcaggacc
      841 agcagtcgct gtcggacgtg gagggcgcat attccagagc tgaagctaca aggggtgctg
      901 gaggcagcag ttctagtccc ccagaaaagg acagcggact gctggacagt gtcttggaca
      961 ctctgttggc gccctcaggt cccgggcaga gccaacccag ccctcccgcc tgcgaggtca
     1021 ccagctcttg gtgcctgttt ggccccgaac ttcccgaaga tccaccggct gcccccgcca
     1081 cccagcgggt gttgtccccg ctcatgagcc ggtccgggtg caaggttgga gacagctccg
     1141 ggacggcagc tgcccataaa gtgctgcccc ggggcctgtc accagcccgg cagctgctgc
     1201 tcccggcctc tgagagccct cactggtccg gggccccagt gaagccgtct ccgcaggccg
     1261 ctgcggtgga ggttgaggag gaggatggct ctgagtccga ggagtctgcg ggtccgcttc
     1321 tgaagggcaa acctcgggct ctgggtggcg cggcggctgg aggaggagcc gcggctgtcc
     1381 cgccgggggc ggcagcagga ggcgtcgccc tggtccccaa ggaagattcc cgcttctcag
     1441 cgcccagggt cgccctggtg gagcaggacg cgccgatggc gcccgggcgc tccccgctgg
     1501 ccaccacggt gatggatttc atccacgtgc ctatcctgcc tctcaatcac gccttattgg
     1561 cagcccgcac tcggcagctg ctggaagacg aaagttacga cggcggggcc ggggctgcca
     1621 gcgcctttgc cccgccgcgg agttcaccct gtgcctcgtc caccccggtc gctgtaggcg
     1681 acttccccga ctgcgcgtac ccgcccgacg ccgagcccaa ggacgacgcg taccctctct
     1741 atagcgactt ccagccgccc gctctaaaga taaaggagga ggaggaaggt gcggaggcct
     1801 ccgcgcgctc cccgcgttcc taccttgtgg ccggtgccaa ccccgcagcc ttcccggatt
     1861 tcccgttggg gccaccgccc ccgctgccgc cgcgagcgac cccatccaga cccggggaag
     1921 cggcggtgac ggccgcaccc gccagtgcct cagtctcgtc tgcgtcctcc tcggggtcga
     1981 ccctggagtg catcctgtac aaagcggagg gcgcgccgcc ccagcagggc ccgttcgcgc
     2041 cgccgccctg caaggcgccg ggcgcgagcg gctgcctgct cccgcgggac ggcctgccct
     2101 ccacctccgc ctctgccgcc gccgccgggg cggcccccgc gctctaccct gcactcggcc
     2161 tcaacgggct cccgcagctc ggctaccagg ccgccgtgct caaggagggc ctgccgcagg
     2221 tctacccgcc ctatctcaac tacctgaggc cggattcaga agccagccag agcccacaat
     2281 acagcttcga gtcattacct cagaagattt gtttaatctg tggggatgaa gcatcaggct
     2341 gtcattatgg tgtccttacc tgtgggagct gtaaggtctt ctttaagagg gcaatggaag
     2401 ggcagcacaa ctacttatgt gctggaagaa atgactgcat cgttgataaa atccgcagaa
     2461 aaaactgccc agcatgtcgc cttagaaagt gctgtcaggc tggcatggtc cttggaggtc
     2521 gaaaatttaa aaagttcaat aaagtcagag ttgtgagagc actggatgct gttgctctcc
     2581 cacagccatt gggcgttcca aatgaaagcc aagccctaag ccagagattc actttttcac
     2641 caggtcaaga catacagttg attccaccac tgatcaacct gttaatgagc attgaaccag
     2701 atgtgatcta tgcaggacat gacaacacaa aacctgacac ctccagttct ttgctgacaa
     2761 gtcttaatca actaggcgag aggcaacttc tttcagtagt caagtggtct aaatcattgc
     2821 caggttttcg aaacttacat attgatgacc agataactct cattcagtat tcttggatga
     2881 gcttaatggt gtttggtcta ggatggagat cctacaaaca tgtcagtggg cagatgctgt
     2941 attttgcacc tgatctaata ctaaatgaac agcggatgaa agaatcatca ttctattcat
     3001 tatgccttac catgtggcag atcccacagg agtttgtcaa gcttcaagtt agccaagaag
     3061 agttcctctg tatgaaagta ttgttacttc ttaatacaat tcctttggaa gggctacgaa
     3121 gtcaaaccca gtttgaggag atgaggtcaa gctacattag agagctcatc aaggcaattg
     3181 gtttgaggca aaaaggagtt gtgtcgagct cacagcgttt ctatcaactt acaaaacttc
     3241 ttgataactt gcatgatctt gtcaaacagc ttcatctgta ctgcttgaat acatttatcc
     3301 agtcccgggc actgagtgtt gaatttccag aaatgatgtc tgaagttatt gctgcacaat
     3361 tacccaagat attggcaggg atggtgaaac cccttctctt tcataaaaag tgaatgtcat
     3421 ctttttcttt taaagaatta aattttgtgg catgtctttt tgttttggtc aggattatga
     3481 ggtcttgagt ttttataatg ttcttctgaa agccttacat ttataacatc atagtgtgta
     3541 aatttaaaag aaaaattgtg aggttctaat tattttcttt tataaagtat aattagaatg
     3601 tttaactgtt ttgtttaccc atattttctt gaagaattta caagattgaa aaagtactaa
     3661 aattgttaaa gtaaactata tcttatccat attatttcat accatgtagg tgaggatttt
     3721 taacttttgc atctaacaaa tcatcgactt aagagaaaaa atcttacatg taataacaca
     3781 aagctattat atgttatttc taggtaactc cctttgtgtc aattatattt ccaaaaatga
     3841 acctttaaaa tggtatgcaa aattttgtct atatatattt gtgtgaggag gaaattcata
     3901 actttcctca gattttcaaa agtattttta atgcaaaaaa tgtagaaaga gtttaaaacc
     3961 actaaaatag attgatgttc ttcaaactag gcaaaacaac tcatatgtta agaccatttt
     4021 ccagattgga aacacaaatc tcttaggaag ttaataagta gattcatatc attatacaaa
     4081 tagtattgtg ggttttgtag gtttttaaaa taaccttttt tggggagaga attgtcctct
     4141 aatgaggtat tgcgagtgga cataagaaat cagaagatta tggcctaact gtactcctta
     4201 ccaactgtgg catgctgaaa gttagtcact cttactgatt ctcaattctc tcacctttga
     4261 aagtagtaaa atatctttcc tgccaattgc tcctttgggt cagagcttat taacatcttt
     4321 tcaaatcaaa ggaaagaaga aagggagagg aggaggaggg agatatcaat tcacatacct
     4381 ttctcctctt tatcctccac tatcatgaat tcagatctgg taccacgcgt atcgataagc
     4441 tgatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc tggaagttgc
     4501 cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
     4561 ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg ggcaagttgg
     4621 gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg cagtggcaca
     4681 atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     4741 cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt ttttttggta
     4801 gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc aggtgatcta
     4861 cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc ccttccctgt
     4921 ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc ataggctacc
     4981 tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc tctcatgcgt
     5041 tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc tgtggaatgt
     5101 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5161 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5221 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5281 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5341 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5401 cttttttgga ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt
     5461 taattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt tcctgtgtga
     5521 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5581 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5641 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7621 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     7681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     8041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     8101 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8161 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8221 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgatctata
     8281 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
     8341 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
     8401 catgtccaat atgaccgcca tgtt
//
