LOCUS       sequence-267788-       10184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, copper-inducible expression of (-)-alpha- bisabolol 
            synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 
            6803. The MrBBS CDS is fused to a C-termial StrepII-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
  TITLE     High density cultivation for efficient sesquiterpenoid biosynthesis 
            in Synechocystis sp. PCC 6803.
  JOURNAL   Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w.
  PUBMED    32246065
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-3"; volume: "10(1):5932. doi"; pages: " 
            10.1038/s41598-020-62681-w"
COMMENT     Sequence Label: pSHDY*in_PpetE:BCD2:MrBBS:Strep
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(160..975)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(160..975)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     883..902
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(976..1067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1093..1110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1154..1945)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVMHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2151..2172
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2175..2246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2262..2289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2803..2811
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4552..4575
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      4596..4667
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4683..4710
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4717..4737
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(4938..5789)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(5776..6615)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7126..8097)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            9336..9423
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      9765..10159
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 ggtaccgagt cgatgtcgac taacgaccct gccctgaacc gacgacaagc tgacgaccgg
       61 gtctccgcaa gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      121 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
      181 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      241 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      301 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      361 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      421 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      481 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
      541 gcggtcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      601 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      661 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      721 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      781 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      841 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      901 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
      961 ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1021 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gcatgcagcg
     1081 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactct cgagagcggc
     1141 cgaagcttag acattatttg ccgactacct tggtgatctc gcctttcacg tagtggacaa
     1201 attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca agataagcct
     1261 gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg
     1321 cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg
     1381 taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg
     1441 tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg
     1501 ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa
     1561 tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa
     1621 attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc ataacaatgg
     1681 tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt
     1741 cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat
     1801 caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca
     1861 gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata
     1921 cttcggcgat caccgcttcc ctcatgatgt ttaactttgt tttagggcga ctgccctgct
     1981 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     2041 gcttggatgc ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa
     2101 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag gaattcgcgg
     2161 ccgcttctag agatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2221 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2281 ctttctgcgt ttttacccta cacttaaacc ccatctcaat acttcccatc aaatccaacc
     2341 taactcatac attccaaact aatctaactt cttccatcat taaccacacc tctctatcat
     2401 cacctattcc cctctatctc cctcaaattc tttcacatat taaaaactta ctcttcctcc
     2461 tttcctcctt aaaatactaa ataactctta aaatagaagg gatagcaagc taatttttat
     2521 gacggcgatc gccaaaaaca aagaaaattc agcaattacc gtgggtagca aaaaatcccc
     2581 atctaaagtt cagtaaatat agctagaaca accaagcatt ttcggcaaag tactattcag
     2641 atagaacgag aaatgagctt gttctatccg cccggggctg aggctgtata atctacgacg
     2701 ggctgtcaaa cattgtgata ccatgggcag aagggcccaa gttcacttaa aaaggagatc
     2761 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
     2821 gctagcttat ccgtatccac tccgtctttc agttcctccc ctctgagtag tgtcaataag
     2881 aactctacaa agcagcacgt aacgcgtaat agcgtgattt ttcacgattc catttggggg
     2941 gaccagtttc tggagtataa ggaaaaattc aacgtcgcta cagagaaaca gctcatcgaa
     3001 gaactcaaag aagaagtgcg aaatgagtta atgattcggg cgtgcaatga ggcctctcgg
     3061 tacattaagc taattcaact cattgatgtt gtggaacgtt taggtttggc ttaccatttt
     3121 gaaaaggaga tcgaggagag tctccaacac atctacgtaa cttatggaca taaatggaca
     3181 aattacaata atattgaaag cctaagtctg tggtttcgac tgctccgaca gaacggtttc
     3241 aacgtatcct ccgacatttt tgaaaatcat atcgatgaga agggaaactt ccaagagagc
     3301 ctatgcaatg acccccaagg aatgctcgct ctgtatgaag ccgcctatat gcgagttgag
     3361 ggggagatca ttctcgacaa agccctggaa tttaccaaac tccatttggg gattattagt
     3421 aacgatccta gctgcgattc tagcttacgt accgaaatta agcaggccct caaacaacct
     3481 ttgcgacgac gtttacctcg cttagaggcc gttcggtaca ttgccatcta tcaacaaaaa
     3541 gcgtctcact ccgaagtatt gttaaagttg gctaaactgg actttaacgt gctccaagag
     3601 atgcacaagg atgagctaag tcagatctgt aagtggtgga aggacttaga catccgtaat
     3661 aaactaccat acgtccgtga ccggctcatc gaagggtact tttggatttt ggggatttat
     3721 tttgaaccac agcatagccg gacgcgaatg ttcttaatga aaacttgtat gtggctgatt
     3781 gtgctggatg atacatttga taactacggg acctatgaag aactcgaaat tttcacgcaa
     3841 gctgtggaac gttggtccat cacttgttta gatgaattgc ccgaatatat gaagctcatt
     3901 taccatgagc aatttcgcgt acaccaagaa atggaagaaa gtctcgaaaa agagggtaag
     3961 gcttaccaga tccattacat taaagaaatg gccaaggaag gtactcggtc cctactctta
     4021 gaagcaaagt ggttgaaaga agggtacatg ccgaccttag atgagtacct ctccaacagt
     4081 ttggtcactt gcggctatgc actcatgacg gcacgctcct atgtcgcgcg tgatgacggt
     4141 attgtcacgg aagacgcctt taaatgggtc gctacccatc ctcccattgt taaagctgcg
     4201 tgtaagatcc tacggttaat ggatgatatt gccacgcata aagaggagca ggaacgaggc
     4261 cacatcgcaa gtagtatcga atgctatcgc aaagagacgg gcgcttctga ggaggaagcg
     4321 tgcatggact tcttaaagca agtagaagat ggttggaaag ttatcaatca ggaaagtctg
     4381 atgcctaccg atgtaccatt ccccttgcta atccccgcaa ttaatctcgc ccgcgtgagc
     4441 gacactttat acaaggataa tgatggttat aatcacgctg acaaggaagt gatcggatac
     4501 atcaaaagcc tgttcgttca tcccatgatt gttgggagcg gatccgggag ttggtctcac
     4561 ccccaatttg agaaataatg accgcggcca ggcatcaaat aaaacgaaag gctcagtcga
     4621 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     4681 ctggctcacc ttcgggtggg cctttctgcg tttatatact agtagcggcc gctgcaggag
     4741 cagaagagca tacatctgga agcaaagcca ggaaagcggc ctatggagct gtgcggcagc
     4801 gctcagtagg caatttttca aaatattgtt aagccttttc tgagcatggt atttttcatg
     4861 gtattaccaa ttagcaggaa aataagccat tgaatataaa agataaaaat gtcttgttta
     4921 caatagagtg gggggggtca gcctgccgcc ttgggccggg tgatgtcgta cttgcccgcc
     4981 gcgaactcgg ttaccgtcca gcccagcgcg accagctccg gcaacgcctc gcgcacccgc
     5041 tggcggcgct tgcgcatggt cgaaccactg gcctctgacg gccagacata gccgcacaag
     5101 gtatctatgg aagccttgcc ggttttgccg gggtcgatcc agccacacag ccgctggtgc
     5161 agcaggcggg cggtttcgct gtccagcgcc cgcacctcgt ccatgctgat gcgcacatgc
     5221 tggccgccac ccatgacggc ctgcgcgatc aaggggttca gggccacgta caggcgcccg
     5281 tccgcctcgt cgctggcgta ctccgacagc agccgaaacc cctgccgctt gcggccattc
     5341 tgggcgatga tggatacctt ccaaaggcgc tcgatgcagt cctgtatgtg cttgagcgcc
     5401 ccaccactat cgacctctgc cccgatttcc tttgccagcg cccgatagct acctttgacc
     5461 accatggcat cagcggtgac ggcctcccac ttgggttcca ggaacagccg gagctgccgt
     5521 ccgccttcgg tcttgggttc cgggccaagc actaggccat taggcccagc catggccacc
     5581 agcccttgca ggatgcgcag atcatcagcg cccagcggct ccgggccgct gaactcgatc
     5641 cgcttgccgt cgccgtagtc atacgtcacg tccagcttgc tgcgcttgcg ctcgccccgc
     5701 ttgagggcac ggaacaggcc gggggccaga cagtgcgccg ggtcgtgccg gacgtggctg
     5761 aggctgtgct tgttcttagg cttcaccacg gggcaccccc ttgctcttgc gctgcctctc
     5821 cagcacggcg ggcttgagca ccccgccgtc atgccgcctg aaccaccgat cagcgaacgg
     5881 tgcgccatag ttggccttgc tcacaccgaa gcggacgaag aaccggcgct ggtcgtcgtc
     5941 cacaccccat tcctcggcct cggcgctggt catgctcgac aggtaggact gccagcggat
     6001 gttatcgacc agtaccgagc tgccccggct ggcctgctgc tggtcgcctg cgcccatcat
     6061 ggccgcgccc ttgctggcat ggtgcaggaa cacgatagag cacccggtat cggcggcgat
     6121 ggcctccatg cgaccgatga cctgggccat ggggccgctg gcgttttctt cctcgatgtg
     6181 gaaccggcgc agcgtgtcca gcaccatcag gcggcggccc tcggcggcgc gcttgaggcc
     6241 gtcgaaccac tccggggcca tgatgttggg caggctgccg atcagcggct ggatcagcag
     6301 gccgtcagcc acggcttgcc gttcctcggc gctgaggtgc gccccaaggg cgtgcaggcg
     6361 gtgatgaatg gcggtgggcg ggtcttcggc gggcaggtag atcaccgggc cggtgggcag
     6421 ttcgcccacc tccagcagat ccggcccgcc tgcaatctgt gcggccagtt gcagggccag
     6481 catggattta ccggcaccac cgggcgacac cagcgccccg accgtaccgg ccaccatgtt
     6541 gggcaaaacg tagtccagcg gtggcggcgc tgctgcgaac gcctccagaa tattgatagg
     6601 cttatgggta gccattgatt gcctcctttg caggcagttg gtggttaggc gctggcgggg
     6661 tcactacccc cgccctgcgc cgctctgagt tcttccaggc actcgcgcag cgcctcgtat
     6721 tcgtcgtcgg tcagccagaa cttgcgctga cgcatccctt tggccttcat gcgctcggca
     6781 tatcgcgctt ggcgtacagc gtcagggctg gccagcaggt cgccggtctg cttgtccttt
     6841 tggtctttca tatcagtcac cgagaaactt gccggggccg aaaggcttgt cttcgcggaa
     6901 caaggacaag gtgcagccgt caaggttaag gctggccata tcagcgactg aaaagcggcc
     6961 agcctcggcc ttgtttgacg tataaccaaa gccaccgggc aaccaatagc ccttgtcact
     7021 tttgatcagg tagaccgacc ctgaagcgct tttttcgtat tccataaaac ccccttctgt
     7081 gcgtgagtac tcatagtata acaggcgtga gtaccaacgc aagcactaca tgctgaaatc
     7141 tggcccgccc ctgtccatgc ctcgctggcg gggtgccggt gcccgtgcca gctcggcccg
     7201 cgcaagctgg acgctgggca gacccatgac cttgctgacg gtgcgctcga tgtaatccgc
     7261 ttcgtggccg ggcttgcgct ctgccagcgc tgggctggcc tcggccatgg ccttgccgat
     7321 ttcctcggca ctgcggcccc ggctggccag cttctgcgcg gcgataaagt cgcacttgct
     7381 gaggtcatca ccgaagcgct tgaccagccc ggccatctcg ctgcggtact cgtccagcgc
     7441 cgtgcgccgg tggcggctaa gctgccgctc gggcagttcg aggctggcca gcctgcgggc
     7501 cttctcctgc tgccgctggg cctgctcgat ctgctggcca gcctgctgca ccagcgccgg
     7561 gccagcggtg gcggtcttgc ccttggattc acgcagcagc acccacggct gataaccggc
     7621 gcgggtggtg tgcttgtcct tgcggttggt gaagcccgcc aagcggccat agtggcggct
     7681 gtcggcgctg gccgggtcgg cgtcgtactc gctggccagc gtccgggcaa tctgcccccg
     7741 aagttcaccg cctgcggcgt cggccacctt gacccatgcc tgatagttct tcgggctggt
     7801 ttccactacc agggcaggct cccggccctc ggctttcatg tcatccaggt caaactcgct
     7861 gaggtcgtcc accagcacca gaccatgccg ctcctgctcg gcgggcctga tatacacgtc
     7921 attgccctgg gcattcatcc gcttgagcca tggcgtgttc tggagcactt cggcggctga
     7981 ccattcccgg ttcatcatct ggccggtggt ggcgtccctg acgccgatat cgaagcgctc
     8041 acagcccatg gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg
     8101 gccaccaagc gcagccagat cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag
     8161 agcaacgatg cgatcagcag caccaccgta ggcatcatgg aagccagcat cacggttagc
     8221 catagcttcc agtgccaccc ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc
     8281 tcggcggcta cctcccgcaa ctctttggcc agctccaccc atgccgcccc tgtctggcgc
     8341 tgggctttca gccactccgc cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc
     8401 tgccgggctt cgtcggccag tgtcgccatg ctctgggcca gcggttcgat ctgctccgct
     8461 aactcgttga tgcctctgga tttcttcact ctgtcgattg cgttcatggt ctattgcctc
     8521 ccggtattcc tgtaagtcga tgatctgggc gttggcggtg tcgatgttca gggccacgtc
     8581 tgcccggtcg gtgcggatgc cccggccttc catctccacc acgttcggcc ccaggtgaac
     8641 accgggcagg cgctcgatgc cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg
     8701 gccagcccgc tctaatgccc ggttggcatg gtcggcccat gcctcgcggg tctgctcaag
     8761 ccatgccttg ggcttgagcg cttcggtctt ctgtgccccg cccttctccg gggtcttgcc
     8821 gttgtaccgc ttgaaccact gagcggcggg ccgctcgatg ccgtcattga tccgctcgga
     8881 gatcatcagg tggcagtgcg ggttctcgcc gccaccggca tggatggcca gcgtatacgg
     8941 caggcgctcg gcaccggtca ggtgctgggc gaactcggac gccagcgcct tctgctggtc
     9001 gagggtcagc tcgaccggca gggcaaattc gacctccttg aacagccgcc cattggcgcg
     9061 ttcatacagg tcggcagcat cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg
     9121 cccggattcg gcgtgcaaga cttcatccat gtcgcgggca tacttgcctt cgcgctggat
     9181 gtagtcggcc ttggccctgg ccgattggcc gcccgacctg ctgccggttt tcgccgtaag
     9241 gtgataaatc gccatgctgc ctcgctgttg cttttgcttt tcggctccat gcaatggccc
     9301 tcggagagcg caccgcccga agggtggccg ttaggccagt ttctcgaaga gaaaccggta
     9361 agtgcgccct cccctacaaa gtagggtcgg gattgccgcc gctgtgcctc catgatagcc
     9421 tacgagacag cacattaaca atggggtgtc aagatggtta aggggagcaa caaggcggcg
     9481 gatcggctgg ccaagctcga agaacaacga gcgcgaatca atgccgaaat tcagcgggtg
     9541 cgggcaaggg aacagcagca agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg
     9601 gccatgattt tggccaaggt gaacagcagc gagtggccgg aggatcggct catggcggca
     9661 atggatgcgt accttgaacg cgaccacgac cgcgccttgt tcggtctgcc gccacgccag
     9721 aaggatgagc cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc
     9781 ccacccttcg ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct
     9841 gcggggtttg gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt
     9901 ggggcggtgt gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata
     9961 ttgggcaagg gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct
    10021 tagataatca tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc
    10081 aggtttgggg gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga
    10141 cagttattgc aggggttcgt gacagttagt acgggagtga cggg
//
