LOCUS       sequence-267791-        9036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF1Balpha 387-1150.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF1Balpha tail 387-1150
FEATURES             Location/Qualifiers
     source          1..9036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..275
                     /label=chicken beta-actin promoter
     primer_bind     1218..1240
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1308..1310,1311..1313,1314..2024)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1353..1374)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1614..1633)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1961..1982
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4455..4589
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4492..4511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4546..4565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4620..4636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4620..4636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4633..4655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4644..4660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4668..4674,4675..4692,4693..4698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4713..4734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4851..4868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5022..5610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5102..5121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5781..6572,6573..6641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6404..6423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6642..6746)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6814..6832
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6870..6892)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6992..7011
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7205..7227
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7219..7236
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7220..7236
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggc
      121 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      181 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      241 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      301 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      361 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      421 aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc
      481 ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      541 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      601 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      661 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      721 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
      781 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
      841 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
      901 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
      961 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1021 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1081 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1141 ccgtcccctt ctccatctcc agcctcgggg ctgtccgcag ggggacggct gccttcgggg
     1201 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt atatcttccc
     1261 ttctctgttc ctccgcagcc cccaagcttg gcgcgcctag gcctaccatg gtgagcaagg
     1321 gcggggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggagga tccggaggcg
     2041 atacatccat ggggtccctc acttcatccc cgtcttcatg ctcacttaat agtcaggcgg
     2101 gcctaacctc tgtgaccagt attcaagaga ggattatgtc tacacctgga ggggaggaag
     2161 ccattgaacg tctgaaggag tcagagaaga tcattgctga gttgaatgaa acctgggaag
     2221 agaaacttcg taaaacagag gccatcagaa tggagagaga agccttgttg gctgagatgg
     2281 gagttgccat acgggaagat gggggaacac ttggcgtttt ctcacctaaa aagactccac
     2341 accttgttaa cctcaatgaa gaccctctaa tgtcggagtg tttgctttat tatatcaagg
     2401 acggaattac aagggttggc caagcagatg ctgagcggcg ccaggacatc gtgctgagtg
     2461 gggcccacat taaagaagag cattgtatct ttcggagtga gagaaacaac actggcgaag
     2521 ttattgtgac cttagagccc tgtgagcgct cagaaaccta tgtgaatggc aagagggtgg
     2581 cccatcctgt tcagctgcgc tcagggaacc gtatcatcat gggtaaaaat catgttttcc
     2641 gctttaacca ccctgagcaa gctcgagcag agcgggagaa gactccctct gctgagaccc
     2701 cctctgagcc tgtggattgg acatttgctc agagagaact tcttgaaaaa caagggattg
     2761 atatgaaaca ggagatggag aaaaggcttc aggaaatgga gatcctttac aaaagggaga
     2821 aggaggaagc tgaccttctt ctggagcagc agaggctgga cgcggattct gatagcgggg
     2881 acgattctga caagagatcc tgtgaagaga gctggaaact gattacttct ctgagagaaa
     2941 agctacctcc cagcaagtta caaaccattg ttaaaaagtg tgggctcccc agcagcggaa
     3001 agaaacgaga accaattaaa atgtatcaga tcccacaaag aaggcgcctg agtaaagatt
     3061 ccaagtgggt caccatctca gatcttaaga tccaggctgt taaagagatt tgctatgagg
     3121 ttgccctcaa tgactttagg cacagtcggc aggagatcga agccttggcc atcgttaaga
     3181 tgaaggaact ctgtgccatg tatgggaaga aagaccccaa tgagcgggac tcctggaggg
     3241 ctgtggccag ggacgtgtgg gacactgttg gggtggggga tgagaagatt gaagatatga
     3301 tggtcactgg gaaagctggg acagatgtag atgacctcaa ggtccacata gacaagctgg
     3361 aagatatttt gcaagaagtc aaaaagcaaa ataatatgaa agatgaagag atcaaagtct
     3421 taaggaataa aatgctcaaa atggagaaag tgttgccatt gattggatca caggaacaga
     3481 agagccaagg aaaccaaaag acagaagagc ccctggttgc tagtgctaac agcatccctg
     3541 atactggtat ccgtaaagga gacagcagag aacttgccaa agaagaacgt gtttcccagc
     3601 tgatgaacgg ggatccggct tttagacgtg gacgtctgcg ttggatgaga caagaacaga
     3661 ttcggtttaa gaacctgcaa cagcaagaga taacaaaaca gcttcgtcgg cagaatgtac
     3721 cccacaggtt catcccccct gagaaccgga agccccgctt tcccttcaag agcaatgcta
     3781 agcacagaaa ctcttggagt cctgggacac atattatcat aacagaagat gaggtaattg
     3841 agctaagagt tccaaaagat gaagaaggaa ggagaggaaa taaggaagaa aaggtgggga
     3901 gagcagcttc tgaagacccc cagtcagcct gcggctccca gggcacgcga agtcagggtc
     3961 acatccaagt cagcaaacag cacattatca gtcaacaagc accacctccg ctacgctgga
     4021 gaagcaattc cctcaataat ggccaaccga aaaacatgcg ttgccaggca gctgcctcct
     4081 cagagtcact aaactcccac agcagtcacc ccaacgctga tgtacaaact ttccaggcca
     4141 agcgtcacat tcaccaacac cgtcagtctt actgtaatta taacactgga gatcaggtag
     4201 agggcagtac agccaactgc tgtcaaaagc agactgacaa gcccagccac tgtagccaat
     4261 ttgagacacc tccaaggatg aggagacagt tctcagcacc caatctcaaa gcgggtcgag
     4321 aaaccacagt ctaatctaga taagtaatga tcataatcag ccatatcaca tctgtagagg
     4381 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4441 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4561 tcatcaatgt atcttatcat gtctggatcc ccgggtaccg agctcgaatt cgtaatcatg
     4621 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4681 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4741 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4801 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4861 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4921 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4981 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5041 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5101 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5161 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5221 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5281 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5341 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5401 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5461 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5521 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5581 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5641 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5701 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5761 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5821 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5881 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5941 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6001 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6061 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6121 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6181 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6241 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6301 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6361 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6421 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6481 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6541 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6601 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6661 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6721 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     6781 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6841 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     6901 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     6961 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     7021 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     7081 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7141 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     7201 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg
     7261 cccacggctc tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat
     7321 ggtcctggct ctgtggcatg gcagtggcac tgtgaggagc cccctaccag cagcacacag
     7381 tggggttggc actgccacgc tccggatgcc gcgctctgat ccaaccccat aatcaaggga
     7441 acccgaattg ccccatcatt gcccccacca cccccatcct gccgggccct cacaccccac
     7501 gctgccttct ggtgacattc cccagcccaa acccacggct tcatggctac cgcggggcat
     7561 ttcccattgc cgccccatta tcagctctgc acacctcccg ctgtacccat gcctcgtggc
     7621 tgcccttctt tgacgtataa tcttctaatt aatacccggc cttgtcaaag tggagcacaa
     7681 acgttaatta attccccagc aggcaggtaa ttaacagtgt gactcccttt ttgctgcgag
     7741 tggggctgat acagagagat gtggcactat ggagcccacg gggtcctggc actgggtgcc
     7801 cacggaggtc cccatgtgct gcagtgtcac cgcctccgag gagacagtat tgtccctgcg
     7861 gtgtccctgc agctcagctc tgtccacagg gccacctcca gtttggaggg gacacaatgc
     7921 agccccgatg caacccatcc tcgcagcatc ccagggacaa agaccccact gcaagaccgc
     7981 acacagggct gggtcccgct cccctaatat ctacagtgct tcttgcatgg ccccttaatc
     8041 aatgcagtta atcagcatgc gctcatgcac cgctctggag ctgcaaagcc cctcgcagcg
     8101 ctgctcacca acaccgcgca ccgccccggc ccagcctgca gcacgcgctg caaacaggaa
     8161 agaaacaaaa tattgcccaa atgtaggcaa aggcattcgg ctgccttgac ctccgccggg
     8221 ccgggccctg cctgactcag ctccttactc agcgctcgct tcctccctcc ggctgccacc
     8281 gccgcagcgc acaccctgac aaagagtggc ccttaacggg ctctgaggtg cacccagcag
     8341 tgcactcagc agtccaaggg ccggcctgga ggtttgcacc gctacgtgct gacattagca
     8401 ttgaacttgg ccctgggtag tgctgcaggc cgggcggggt gggtgtagag agtgcagcgc
     8461 gcgttgcacc cggtgcccct tcccctccct tgcatcccag caggctgcac cccagcacca
     8521 ggcccgtgca tgcatgctcc tggtgttatt gcagcctggt gcatgcatgc gtcttgatgg
     8581 tgcagcgctg tgcatgcatc ctccttggtg tgtagcagtc tagtgcatgc atacccctcg
     8641 gtgttattgc tgctctgtgc acgcacgctc attgtatcac ttcatcccag tgcatgcact
     8701 cacactggag cgattgctgc tcggtgcacg cacactcatt gtatcactgc agctcagtgg
     8761 ctgcacgcac accggtgtta ttgctgctcg gtgcgtgcat gcacatcagt gtcgctgcag
     8821 ctcagtgcat gcacgctcat tgcccatcgc tatccctgcc tctcctgctg gcgctccccg
     8881 ggaggtgact tcaaggggac cgcaggacca cctcgggggt ggggggaggg ctgcacacgc
     8941 ggaccccgct ccccctcccc aacaaagcac tgtggaatca aaaagggggg aggggggatg
     9001 gaggggcgcg tcacaccccc gccccacacc ctcacc
//
