LOCUS       sequence-267793-       10898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF1Bbeta 387-1170.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10898)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 10898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF1Bbeta tail 387-1770
FEATURES             Location/Qualifiers
     source          1..10898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1309..1311,1312..1314,1315..2025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1354..1375)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1615..1634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1962..1983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6317..6451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6354..6373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6408..6427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6482..6498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6482..6498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6495..6517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6506..6522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6530..6536,6537..6554,6555..6560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6575..6596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6713..6730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6884..7472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6964..6983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7643..8434,8435..8503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8266..8285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8504..8608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8676..8694
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8732..8754)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8854..8873
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9067..9089
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9081..9098
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9082..9098
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccta ggcctaccat ggtgagcaag
     1321 ggcggggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggagg atccggaggc
     2041 gatacatcca tggggtccct cacttcatcc ccgtcttcat gctcacttaa tagtcaggcg
     2101 ggcctaacct ctgtgaccag tattcaagag aggattatgt ctacacctgg aggggaggaa
     2161 gccattgaac gtctgaagga gtcagagaag atcattgctg agttgaatga aacctgggaa
     2221 gagaaacttc gtaaaacaga ggccatcaga atggagagag aagccttgtt ggctgagatg
     2281 ggagttgcca tacgggaaga tgggggaaca cttggcgttt tctcacctaa aaagactcca
     2341 caccttgtta acctcaatga agaccctcta atgtcggagt gtttgcttta ttatatcaag
     2401 gacggaatta caagggttgg ccaagcagat gctgagcggc gccaggacat cgtgctgagt
     2461 ggggcccaca ttaaagaaga gcattgtatc tttcggagtg agagaaacaa cactggcgaa
     2521 gttattgtga ccttagagcc ctgtgagcgc tcagaaacat acgtgaatgg caagagggtg
     2581 gcccatcctg ttcagctgcg ctcagggaac cgtatcatca tgggtaaaaa ccatgttttt
     2641 cgttttaacc accctgaaca agcgcgagca gaaagggaaa agactccctc agctgagacc
     2701 ccctctgagc ctgtggactg gacctttgct cagagagagc ttctcgaaaa acaaggaatt
     2761 gatatgaaac aggagatgga aaagaggctt caggaaatgg agatccttta caaaaaggag
     2821 aaggaggaag ccgatcttct cctggagcag cagagactgg actacgagag taaattgcag
     2881 gccttgcaga ggcaggttga gactcgatct ctggctgcag agacaacgga agaggaggaa
     2941 gaggaggagg aagttccttg gacacagcat gaatttgagt tggcccagtg ggcgttccgg
     3001 aaatggaagt ctcaccagtt tacttcatta agggacttac tctggggcaa tgcggtgtac
     3061 ctgaaggaag ccaatgccat cagtgtggaa ctaaagaaaa aggtgcagtt ccagtttgtt
     3121 ctgctgactg acacgctgta ctcccctgtg cctcctgaac tgcttccctc ggagatggag
     3181 aagacgcacg aggacagacc tttccctcgg actgtggtgg cggtggaggt ccaggatctg
     3241 aagaatggag caacacatta ttggtctttg gataaactca agcagagact ggatctgatg
     3301 cgagagatgt atgacagggc aggggaggtg gcctccagtg cccaagatga cagcgagacg
     3361 accatgaccg gcagcgaccc attctacgat cggttccact ggttcaaact tgtgggaagc
     3421 tcccctattt tccacggctg tgtgaatgag cgccttgccg accgcacacc ctcccccact
     3481 ttttccacgg ccgattccga catcactgaa ctggctgacg agcagcagga tgccatggag
     3541 gactttgatg atgaggcatt cgtggatgac accggctctg acgcagggac ggaagaggga
     3601 tcagagctct tcagtgatgg gcatgaccca ttttacgacc gatccccttg gttcatttta
     3661 gtaggaaggg catttgtgta cttgagcaac ctgctgtgtc cggtgcccct gattcacagg
     3721 gtggccattg tcagtgagaa gggggaagtc cggggatttc ttcgtgtggc tgtgcaggcc
     3781 attgcagcgg atgaagaagc ccctgattat ggctctggaa tccggcagtc gggaacagct
     3841 aaaatatctt ttgataatga atactttaat cagagtgact tctcgtcagc tgcaatgact
     3901 cgttctggtc tgtccttgga ggagctgagg attgtggaag gtcagggcca gagttctgaa
     3961 gtcatcagtc ctccagaaga agtcaatcga atgaacgacc tggatttgaa gtcaggcact
     4021 ttgctggatg ggaagatggt gatggaaggg ttttctgaag agattggtaa ccacctgaag
     4081 ctgggcagtg ccttcacttt ccgagtcact gtgctccaag ccagcgggat ccttccagag
     4141 tatgcagaca tcttctgtcg gttcaacttt ttgcatcggc atgatgaagc gttctccact
     4201 gaacccctca agaacaatgg aagaggaagt cccctgggct tctaccatgt acagaacatt
     4261 gcggtggagg tcacagagtc gtttgtggac tacatcaaaa ccaagcccat tgtattcgag
     4321 gtctttgggc actaccagca gcacccactc catctgcaag gacaagatct taacagtcca
     4381 cctcagccat ctcggagatt cttccccccg cccatgccac tctccaagcc agttccagct
     4441 attaagttaa acaccatgaa caaaaccacc cttgggcaaa gcatgagcaa gtacgacctt
     4501 ctggtttggt ttgagatcag tgaactggag cctactggag agtatattcc agctgtggtt
     4561 gaccatactg caggcctgcc ctgccagggg acatttttgc tgcaccaggg catccagcga
     4621 cggatcacag tgactatcat ccacgagaag ggcagtgagc tccactggaa agatgtccgt
     4681 gagctggtgg taggtcggat taggaataaa ccggaggtag atgaagctgc agttgatgct
     4741 gttctttccc tgaacatcat ctctgctaag tccctgaagg ctgcccacag ctccagcagg
     4801 accttctacc gtttcgaggc agtatgggac agctccctac ataactccct ccttctgaac
     4861 cgagtgacac cctatggaga aaagatctac atgaccttat ctgcttatct ggagttggat
     4921 cattgcatcc agccggccgt catcactaag gatgtgtgca tggtcttcta ttcccgagat
     4981 gccaagatct caccaccgcg ttctctgcga aacctctttg gtagtggcta ctcaaagtca
     5041 ccagactcca accgagtgac tgggatctat gagctgagct tatgcaaaat ggcagatact
     5101 ggaagcccag gcatgcagag gcggaggaga aaagtcttgg atacctcagt ggcatatgtg
     5161 cggggagagg agaacctagc gggctggcga cctcgtggag acagcctcat tctggaacac
     5221 cagtgggagc tggagaagct ggagctgctg catgaggtgg aaaaaactcg ccacttcttg
     5281 ctgcttcggg agagacttgg tgacagcgtc cctaagtctc tgagtgactc actgtccccc
     5341 agcctgagca gtggaaccct tagcacctcc accagcatct cctctcagat ctcaaccacc
     5401 acctttgaaa gcgccatcac accgagcgag agcagtggct acgactcggc agacgttgaa
     5461 agcctggtcg accgggagaa agagctggct acaaagtgcc tgcagcttct cacccacact
     5521 ttcaaccgag aattcagcca ggtgcacggc agcatcagtg actgtaagtt gtctgacatc
     5581 tctccaattg gacgggaccc ctctgtgtcc agtttcagca gttccaccct cacgccctcc
     5641 tccacttgcc cctccttggt ggactccagg agcagctcta tggatcagaa gaccccagaa
     5701 gccaactctc gtgcctccag tccctgccaa gaatttgaac agtttcagat cgtcccaact
     5761 gtggagacac cctatttggc ccgagcaggg aagaatgaat ttctcaatct tgttccagac
     5821 attgaagaag tcagagcggg atcagtggta tctaagaaag gatacctgca tttcaaagag
     5881 ccactttcca gtaactgggc taaacatttc gttgtggttc gtcgccctta cgtcttcatc
     5941 tataacagtg acgaagaccc agtagagcgt ggcatcatta acctgtctac agcacaggtg
     6001 gagtacagcg aggaccagca ggccatggtg aagacaccta atacttttgc tgtatgcaca
     6061 aagcaccgtg gggtccttct gcaagctctc aatgacaaag acatgaatga ctggttatat
     6121 gcctttaacc cacttctggc tggcacaata aggtcaaaac tctctcgcag atgcccgaac
     6181 cagccaaagt actgactcta gataagtaat gatcataatc agccatatca catctgtaga
     6241 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     6301 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6421 actcatcaat gtatcttatc atgtctggat ccccgggtac cgagctcgaa ttcgtaatca
     6481 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6541 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6601 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6661 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8641 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8701 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8761 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8821 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8881 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8941 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     9001 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     9061 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagttggga tctttgcatt
     9121 ggcccacggc tctcaggatg gggatgctcc ccttcagcac ccggttcccc ttggaaactg
     9181 atggtcctgg ctctgtggca tggcagtggc actgtgagga gccccctacc agcagcacac
     9241 agtggggttg gcactgccac gctccggatg ccgcgctctg atccaacccc ataatcaagg
     9301 gaacccgaat tgccccatca ttgcccccac cacccccatc ctgccgggcc ctcacacccc
     9361 acgctgcctt ctggtgacat tccccagccc aaacccacgg cttcatggct accgcggggc
     9421 atttcccatt gccgccccat tatcagctct gcacacctcc cgctgtaccc atgcctcgtg
     9481 gctgcccttc tttgacgtat aatcttctaa ttaatacccg gccttgtcaa agtggagcac
     9541 aaacgttaat taattcccca gcaggcaggt aattaacagt gtgactccct ttttgctgcg
     9601 agtggggctg atacagagag atgtggcact atggagccca cggggtcctg gcactgggtg
     9661 cccacggagg tccccatgtg ctgcagtgtc accgcctccg aggagacagt attgtccctg
     9721 cggtgtccct gcagctcagc tctgtccaca gggccacctc cagtttggag gggacacaat
     9781 gcagccccga tgcaacccat cctcgcagca tcccagggac aaagacccca ctgcaagacc
     9841 gcacacaggg ctgggtcccg ctcccctaat atctacagtg cttcttgcat ggccccttaa
     9901 tcaatgcagt taatcagcat gcgctcatgc accgctctgg agctgcaaag cccctcgcag
     9961 cgctgctcac caacaccgcg caccgccccg gcccagcctg cagcacgcgc tgcaaacagg
    10021 aaagaaacaa aatattgccc aaatgtaggc aaaggcattc ggctgccttg acctccgccg
    10081 ggccgggccc tgcctgactc agctccttac tcagcgctcg cttcctccct ccggctgcca
    10141 ccgccgcagc gcacaccctg acaaagagtg gcccttaacg ggctctgagg tgcacccagc
    10201 agtgcactca gcagtccaag ggccggcctg gaggtttgca ccgctacgtg ctgacattag
    10261 cattgaactt ggccctgggt agtgctgcag gccgggcggg gtgggtgtag agagtgcagc
    10321 gcgcgttgca cccggtgccc cttcccctcc cttgcatccc agcaggctgc accccagcac
    10381 caggcccgtg catgcatgct cctggtgtta ttgcagcctg gtgcatgcat gcgtcttgat
    10441 ggtgcagcgc tgtgcatgca tcctccttgg tgtgtagcag tctagtgcat gcatacccct
    10501 cggtgttatt gctgctctgt gcacgcacgc tcattgtatc acttcatccc agtgcatgca
    10561 ctcacactgg agcgattgct gctcggtgca cgcacactca ttgtatcact gcagctcagt
    10621 ggctgcacgc acaccggtgt tattgctgct cggtgcgtgc atgcacatca gtgtcgctgc
    10681 agctcagtgc atgcacgctc attgcccatc gctatccctg cctctcctgc tggcgctccc
    10741 cgggaggtga cttcaagggg accgcaggac cacctcgggg gtggggggag ggctgcacac
    10801 gcggaccccg ctccccctcc ccaacaaagc actgtggaat caaaaagggg ggagggggga
    10861 tggaggggcg cgtcacaccc ccgccccaca ccctcacc
//
