LOCUS       sequence-267796-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (D126 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 D126 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        46..425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        426..629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     580..600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     674..693
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        674..692
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1096..1116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1132..1152
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4582..4629
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4630..4653
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4663..4719
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4720..5118
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5189..5206)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5195..5419
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5465..5893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5552..5571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5762..5783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5902..5922)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5907..6236
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6087..6222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6149..6168
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6303..7097
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6357..6376)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6967..6986
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7271..7392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7308..7327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7362..7381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7441..7457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7441..7457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7454..7476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7465..7481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7489..7495,7496..7513,7514..7519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7534..7555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7672..7689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7843..8431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7923..7942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8602..9393,9394..9462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9225..9244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9463..9567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9642..9661)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
       61 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      421 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      481 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      541 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      601 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
      661 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgccaccatg
      721 aagcgaaatt acattctggg gctggccatt ggcattacat cagtgggcta tggcatcatt
      781 gactacgaga caagggacgt gatcgacgcc ggcgtgagac tgttcaagga ggccaacgtg
      841 gagaacaatg agggccggag atccaagagg ggagcaaggc gcctgaagcg gagaaggcgc
      901 cacagaatcc agagagtgaa gaagctgctg ttcgattaca acctgctgac cgaccactcc
      961 gagctgtctg gcatcaatcc ttatgaggcc agagtgaagg gcctgtccca gaagctgtct
     1021 gaggaggagt ttagcgccgc cctgctgcac ctggcaaaga ggagaggcgt gcacaacgtg
     1081 aatgaggtgg aggagccgaa gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag
     1141 aagcgcaaag tgggcagcga cgcgctggac gatttcgatc tggacatgct gggcagcgat
     1201 gcgctggatg atttcggcgg tggcagctct gaggtggagt tttcccacga gtactggatg
     1261 agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc tgtgggagcc
     1321 gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac
     1381 gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac
     1441 tacagactga ttgacgccac cctgtacggc acattcgagc cttgcgtgat gtgcgccggc
     1501 gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc
     1561 gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt
     1621 accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt tcggatgcct
     1681 agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgacggcgg tagcggcggt
     1741 agcggtggta gcggcggcag cggtggtagc ggtggcagcg gtggtgagga caccggcaac
     1801 gagctgtcca caaaggagca gatcagccgc aattccaagg ccctggagga gaagtatgtg
     1861 gccgagctgc agctggagcg gctgaagaag gatggcgagg tgaggggctc catcaatcgc
     1921 ttcaagacct ctgactacgt gaaggaggcc aagcagctgc tgaaggtgca gaaggcctac
     1981 caccagctgg atcagtcctt tatcgataca tatatcgacc tgctggagac aaggcgcaca
     2041 tactatgagg gaccaggaga gggctctccc ttcggctgga aggacatcaa ggagtggtac
     2101 gagatgctga tgggccactg cacctatttt ccagaggagc tgagaagcgt gaagtacgcc
     2161 tataacgccg atctgtacaa cgccctgaat gacctgaaca acctggtcat caccagggat
     2221 gagaacgaga agctggagta ctatgagaag ttccagatca tcgagaacgt gttcaagcag
     2281 aagaagaagc ctacactgaa gcagatcgcc aaggagatcc tggtgaacga ggaggacatc
     2341 aagggctacc gcgtgacctc cacaggcaag ccagagttca ccaatctgaa ggtgtatcac
     2401 gatatcaagg acatcacagc ccggaaggag atcatcgaga acgccgagct gctggatcag
     2461 atcgccaaga tcctgaccat ctatcagagc tccgaggaca tccaggagga gctgaccaac
     2521 ctgaatagcg agctgacaca ggaggagatc gagcagatca gcaatctgaa gggctacacc
     2581 ggcacacaca acctgagcct gaaggccatc aatctgatcc tggatgagct gtggcacaca
     2641 aacgacaatc agatcgccat ctttaaccgg ctgaagctgg tgccaaagaa ggtggacctg
     2701 tcccagcaga aggagatccc aaccacactg gtggacgatt tcatcctgtc tcccgtggtg
     2761 aagcggagct tcatccagag catcaaagtg atcaacgcca tcatcaagaa gtacggcctg
     2821 cccaatgata tcatcatcga gctggccagg gagaagaact ccaaggacgc ccagaagatg
     2881 atcaatgaga tgcagaagag gaaccgccag accaatgagc ggatcgagga gatcatcaga
     2941 accacaggca aggagaacgc caagtacctg atcgagaaga tcaagctgca cgatatgcag
     3001 gagggcaagt gtctgtattc tctggaggcc atccctctgg aggacctgct gaacaatcca
     3061 ttcaactacg aggtggatca catcatcccc cggagcgtga gcttcgacaa ttcttttaac
     3121 aataaggtgc tggtgaagca ggaggagaac agcaagaagg gcaataggac ccctttccag
     3181 tacctgtcta gctccgattc taagatcagc tacgagacat tcaagaagca catcctgaat
     3241 ctggccaagg gcaagggccg catcagcaag accaagaagg agtacctgct ggaggagcgg
     3301 gacatcaaca gattctccgt gcagaaggac ttcatcaacc ggaatctggt ggacaccaga
     3361 tacgccacac gcggcctgat gaatctgctg cggtcttatt tcagagtgaa caatctggat
     3421 gtgaaggtga agagcatcaa cggcggcttc acctcctttc tgcggagaaa gtggaagttt
     3481 aagaaggagc gcaacaaggg ctataagcac cacgccgagg atgccctgat catcgccaat
     3541 gccgacttca tctttaagga gtggaagaag ctggacaagg ccaagaaagt gatggagaac
     3601 cagatgttcg aggagaagca ggccgagagc atgcccgaga tcgagacaga gcaggagtac
     3661 aaggagattt tcatcacacc tcaccagatc aagcacatca aggacttcaa ggactacaag
     3721 tattctcaca gggtggataa gaagcccaac cgcgagctga tcaatgacac cctgtatagc
     3781 acacggaagg acgataaggg caataccctg atcgtgaaca atctgaacgg cctgtacgac
     3841 aaggataatg acaagctgaa gaagctgatc aacaagtctc ccgagaagct gctgatgtac
     3901 caccacgatc ctcagacata tcagaagctg aagctgatca tggagcagta cggcgacgag
     3961 aagaacccac tgtataagta ctatgaggag acaggcaact acctgacaaa gtatagcaag
     4021 aaggataatg gccccgtgat caagaagatc aagtactatg gcaacaagct gaatgcccac
     4081 ctggacatca ccgacgatta ccctaactct cgcaataagg tggtgaagct gagcctgaag
     4141 ccataccggt tcgacgtgta cctggacaac ggcgtgtata agtttgtgac agtgaagaat
     4201 ctggatgtga tcaagaagga gaactactat gaggtgaaca gcaagtgcta cgaggaggcc
     4261 aagaagctga agaagatcag caaccaggcc gagttcatcg cctcttttta caacaatgac
     4321 ctgatcaaga tcaatggcga gctgtataga gtgatcggcg tgaacaatga tctgctgaac
     4381 agaatcgaag tgaatatgat cgacatcacc tacagggagt atctggagaa catgaatgat
     4441 aagaggcccc ctcgcatcat caagaccatc gcctctaaga cacagagcat caagaagtac
     4501 agcacagaca tcctggggaa cctgtatgaa gtcaagagca agaaacatcc tcagattatc
     4561 aagaaaggct ctggtggttc taagcgacct gccgccacaa agaaggctgg acaggctaag
     4621 aagaagaaag attacaaaga cgatgacgat aagggatccg gcgcaacaaa cttctctctg
     4681 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tggccaagcc tttgtctcaa
     4741 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     4801 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     4861 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     4921 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     4981 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     5041 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     5101 tatgtgtggg agggctaaga attctgcaga tatccagcac agtggcggcc gctcgagtct
     5161 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     5221 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5281 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5341 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5401 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5461 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5521 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5581 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5641 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5701 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5761 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5881 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6001 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6061 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6121 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6181 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6241 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6301 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6361 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6421 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6481 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6541 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6601 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6661 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6721 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6781 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6841 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6901 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6961 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     7021 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     7081 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     7141 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7201 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7261 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7381 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8281 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9601 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     9661 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     9721 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     9781 agggtta
//
