LOCUS       sequence-267797-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E739 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E739 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        156..535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        536..739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     690..710
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     784..803
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        784..802
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3045..3065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3081..3101
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4692..4739
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4740..4763
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4773..4829
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4830..5228
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5299..5316)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5305..5529
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5575..6003
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5662..5681)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5872..5893
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6012..6032)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6017..6346
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6197..6332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6259..6278
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6413..7207
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6467..6486)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7077..7096
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7381..7502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7418..7437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7472..7491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7551..7567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7551..7567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7564..7586)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7575..7591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7599..7605,7606..7623,7624..7629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7644..7665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7782..7799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7953..8541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8033..8052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8712..9503,9504..9572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9335..9354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9573..9677)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9752..9771)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
       61 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
      121 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      781 aattaatacg actcactata gggagaccca agctggctag cgccaccatg aagcgaaatt
      841 acattctggg gctggccatt ggcattacat cagtgggcta tggcatcatt gactacgaga
      901 caagggacgt gatcgacgcc ggcgtgagac tgttcaagga ggccaacgtg gagaacaatg
      961 agggccggag atccaagagg ggagcaaggc gcctgaagcg gagaaggcgc cacagaatcc
     1021 agagagtgaa gaagctgctg ttcgattaca acctgctgac cgaccactcc gagctgtctg
     1081 gcatcaatcc ttatgaggcc agagtgaagg gcctgtccca gaagctgtct gaggaggagt
     1141 ttagcgccgc cctgctgcac ctggcaaaga ggagaggcgt gcacaacgtg aatgaggtgg
     1201 aggaggacac cggcaacgag ctgtccacaa aggagcagat cagccgcaat tccaaggccc
     1261 tggaggagaa gtatgtggcc gagctgcagc tggagcggct gaagaaggat ggcgaggtga
     1321 ggggctccat caatcgcttc aagacctctg actacgtgaa ggaggccaag cagctgctga
     1381 aggtgcagaa ggcctaccac cagctggatc agtcctttat cgatacatat atcgacctgc
     1441 tggagacaag gcgcacatac tatgagggac caggagaggg ctctcccttc ggctggaagg
     1501 acatcaagga gtggtacgag atgctgatgg gccactgcac ctattttcca gaggagctga
     1561 gaagcgtgaa gtacgcctat aacgccgatc tgtacaacgc cctgaatgac ctgaacaacc
     1621 tggtcatcac cagggatgag aacgagaagc tggagtacta tgagaagttc cagatcatcg
     1681 agaacgtgtt caagcagaag aagaagccta cactgaagca gatcgccaag gagatcctgg
     1741 tgaacgagga ggacatcaag ggctaccgcg tgacctccac aggcaagcca gagttcacca
     1801 atctgaaggt gtatcacgat atcaaggaca tcacagcccg gaaggagatc atcgagaacg
     1861 ccgagctgct ggatcagatc gccaagatcc tgaccatcta tcagagctcc gaggacatcc
     1921 aggaggagct gaccaacctg aatagcgagc tgacacagga ggagatcgag cagatcagca
     1981 atctgaaggg ctacaccggc acacacaacc tgagcctgaa ggccatcaat ctgatcctgg
     2041 atgagctgtg gcacacaaac gacaatcaga tcgccatctt taaccggctg aagctggtgc
     2101 caaagaaggt ggacctgtcc cagcagaagg agatcccaac cacactggtg gacgatttca
     2161 tcctgtctcc cgtggtgaag cggagcttca tccagagcat caaagtgatc aacgccatca
     2221 tcaagaagta cggcctgccc aatgatatca tcatcgagct ggccagggag aagaactcca
     2281 aggacgccca gaagatgatc aatgagatgc agaagaggaa ccgccagacc aatgagcgga
     2341 tcgaggagat catcagaacc acaggcaagg agaacgccaa gtacctgatc gagaagatca
     2401 agctgcacga tatgcaggag ggcaagtgtc tgtattctct ggaggccatc cctctggagg
     2461 acctgctgaa caatccattc aactacgagg tggatcacat catcccccgg agcgtgagct
     2521 tcgacaattc ttttaacaat aaggtgctgg tgaagcagga ggagaacagc aagaagggca
     2581 ataggacccc tttccagtac ctgtctagct ccgattctaa gatcagctac gagacattca
     2641 agaagcacat cctgaatctg gccaagggca agggccgcat cagcaagacc aagaaggagt
     2701 acctgctgga ggagcgggac atcaacagat tctccgtgca gaaggacttc atcaaccgga
     2761 atctggtgga caccagatac gccacacgcg gcctgatgaa tctgctgcgg tcttatttca
     2821 gagtgaacaa tctggatgtg aaggtgaaga gcatcaacgg cggcttcacc tcctttctgc
     2881 ggagaaagtg gaagtttaag aaggagcgca acaagggcta taagcaccac gccgaggatg
     2941 ccctgatcat cgccaatgcc gacttcatct ttaaggagtg gaagaagctg gacaaggcca
     3001 agaaagtgat ggagaaccag atgttcgagg agaagcaggc cgagccgaag aagaaacgta
     3061 aggtgggtag cagcggtagc ccgaaaaaga agcgcaaagt gggcagcgac gcgctggacg
     3121 atttcgatct ggacatgctg ggcagcgatg cgctggatga tttcggcggt ggcagctctg
     3181 aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc aagagggcac
     3241 gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga gtgatcggcg
     3301 agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa attatggccc
     3361 tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc ctgtacggca
     3421 cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg
     3481 tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac gtgctgcact
     3541 accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat gaatgtgccg
     3601 ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag aagaaggccc
     3661 agagctccac cgacggcggt agcggcggta gcggtggtag cggcggcagc ggtggtagcg
     3721 gtggcagcgg tggtgagagc atgcccgaga tcgagacaga gcaggagtac aaggagattt
     3781 tcatcacacc tcaccagatc aagcacatca aggacttcaa ggactacaag tattctcaca
     3841 gggtggataa gaagcccaac cgcgagctga tcaatgacac cctgtatagc acacggaagg
     3901 acgataaggg caataccctg atcgtgaaca atctgaacgg cctgtacgac aaggataatg
     3961 acaagctgaa gaagctgatc aacaagtctc ccgagaagct gctgatgtac caccacgatc
     4021 ctcagacata tcagaagctg aagctgatca tggagcagta cggcgacgag aagaacccac
     4081 tgtataagta ctatgaggag acaggcaact acctgacaaa gtatagcaag aaggataatg
     4141 gccccgtgat caagaagatc aagtactatg gcaacaagct gaatgcccac ctggacatca
     4201 ccgacgatta ccctaactct cgcaataagg tggtgaagct gagcctgaag ccataccggt
     4261 tcgacgtgta cctggacaac ggcgtgtata agtttgtgac agtgaagaat ctggatgtga
     4321 tcaagaagga gaactactat gaggtgaaca gcaagtgcta cgaggaggcc aagaagctga
     4381 agaagatcag caaccaggcc gagttcatcg cctcttttta caacaatgac ctgatcaaga
     4441 tcaatggcga gctgtataga gtgatcggcg tgaacaatga tctgctgaac agaatcgaag
     4501 tgaatatgat cgacatcacc tacagggagt atctggagaa catgaatgat aagaggcccc
     4561 ctcgcatcat caagaccatc gcctctaaga cacagagcat caagaagtac agcacagaca
     4621 tcctggggaa cctgtatgaa gtcaagagca agaaacatcc tcagattatc aagaaaggct
     4681 ctggtggttc taagcgacct gccgccacaa agaaggctgg acaggctaag aagaagaaag
     4741 attacaaaga cgatgacgat aagggatccg gcgcaacaaa cttctctctg ctgaaacaag
     4801 ccggagatgt cgaagagaat cctggaccga tggccaagcc tttgtctcaa gaagaatcca
     4861 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     4921 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     4981 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     5041 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     5101 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     5161 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     5221 agggctaaga attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     5281 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5341 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5401 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5461 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5521 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5581 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5641 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5701 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5761 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5821 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5881 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5941 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6001 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6361 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6421 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6481 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6541 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6601 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6661 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6721 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6781 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6841 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6901 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     6961 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7021 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7081 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7141 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7201 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7261 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7321 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7381 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7441 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7501 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7561 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7621 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7681 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7741 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7801 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7861 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7921 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7981 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8041 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8101 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8161 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8221 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8281 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8341 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8401 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8461 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8521 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8581 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8641 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8821 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9661 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9721 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9781 gccagta
//
