LOCUS       sequence-267798-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E745 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E745 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        169..548
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        549..752
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     703..723
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     797..816
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        797..815
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3076..3096
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3112..3132
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4705..4752
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4753..4776
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4786..4842
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4843..5241
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5312..5329)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5318..5542
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5588..6016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5675..5694)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5885..5906
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6025..6045)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6030..6359
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6210..6345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6272..6291
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6426..7220
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6480..6499)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7090..7109
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7394..7515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7431..7450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7485..7504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7564..7580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7564..7580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7577..7599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7588..7604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7612..7618,7619..7636,7637..7642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7657..7678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7795..7812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7966..8554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8046..8065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8725..9516,9517..9585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9348..9367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9586..9690)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9765..9784)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
       61 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
      121 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      181 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      241 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      301 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      361 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      421 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      481 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      541 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      601 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      661 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      721 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
      781 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgccacc
      841 atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc
      901 attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac
      961 gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg
     1021 cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac
     1081 tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg
     1141 tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac
     1201 gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc
     1261 aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag
     1321 gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc
     1381 aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca
     1441 tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc
     1501 ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt
     1561 ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat
     1621 gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag
     1681 ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc
     1741 aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag
     1801 ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag
     1861 atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc
     1921 tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc
     1981 gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc
     2041 aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg
     2101 ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg
     2161 gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg
     2221 atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg
     2281 gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag
     2341 accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg
     2401 atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc
     2461 atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc
     2521 cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac
     2581 agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc
     2641 tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag
     2701 accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac
     2761 ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg
     2821 cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc
     2881 acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac
     2941 cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag
     3001 ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc
     3061 atgcccgaga tcgagccgaa gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag
     3121 aagcgcaaag tgggcagcga cgcgctggac gatttcgatc tggacatgct gggcagcgat
     3181 gcgctggatg atttcggcgg tggcagctct gaggtggagt tttcccacga gtactggatg
     3241 agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc tgtgggagcc
     3301 gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac
     3361 gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac
     3421 tacagactga ttgacgccac cctgtacggc acattcgagc cttgcgtgat gtgcgccggc
     3481 gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc
     3541 gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt
     3601 accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt tcggatgcct
     3661 agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgacggcgg tagcggcggt
     3721 agcggtggta gcggcggcag cggtggtagc ggtggcagcg gtggtgagac agagcaggag
     3781 tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac
     3841 aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat
     3901 agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac
     3961 gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg
     4021 taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac
     4081 gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc
     4141 aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc
     4201 cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg
     4261 aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag
     4321 aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag
     4381 gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat
     4441 gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg
     4501 aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat
     4561 gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag
     4621 tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt
     4681 atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct
     4741 aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct
     4801 ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct
     4861 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
     4921 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
     4981 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
     5041 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
     5101 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
     5161 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
     5221 ggttatgtgt gggagggcta agaattctgc agatatccag cacagtggcg gccgctcgag
     5281 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5341 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5401 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5461 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5521 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     5581 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5641 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5701 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5761 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5821 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5881 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5941 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6001 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     6061 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     6121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     6301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     6361 agctcccggg agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt
     6421 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     6481 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     6541 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     6601 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     6661 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     6721 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     6781 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     6841 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     6901 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     6961 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     7021 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     7081 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     7141 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     7201 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     7261 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7321 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7381 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7441 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7501 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7561 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7621 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7681 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7741 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7801 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7861 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8161 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8401 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9721 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     9781 gccgcat
//
