LOCUS       sequence-267799-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (N730 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 N730 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        124..503
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        504..707
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     658..678
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     752..771
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        752..770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2986..3006
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3022..3042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4660..4707
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4708..4731
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4741..4797
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4798..5196
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5267..5284)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5273..5497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5543..5971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5630..5649)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5840..5861
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5980..6000)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5985..6314
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6165..6300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6227..6246
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6381..7175
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6435..6454)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7045..7064
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7349..7470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7386..7405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7440..7459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7519..7535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7519..7535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7532..7554)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7543..7559
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7567..7573,7574..7591,7592..7597))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7612..7633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7750..7767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7921..8509)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8001..8020)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8680..9471,9472..9540))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9303..9322
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9541..9645)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9720..9739)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
       61 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      121 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      181 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      241 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      301 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      361 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      421 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      481 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      541 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      601 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      661 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
      721 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
      781 ctggctagcg ccaccatgaa gcgaaattac attctggggc tggccattgg cattacatca
      841 gtgggctatg gcatcattga ctacgagaca agggacgtga tcgacgccgg cgtgagactg
      901 ttcaaggagg ccaacgtgga gaacaatgag ggccggagat ccaagagggg agcaaggcgc
      961 ctgaagcgga gaaggcgcca cagaatccag agagtgaaga agctgctgtt cgattacaac
     1021 ctgctgaccg accactccga gctgtctggc atcaatcctt atgaggccag agtgaagggc
     1081 ctgtcccaga agctgtctga ggaggagttt agcgccgccc tgctgcacct ggcaaagagg
     1141 agaggcgtgc acaacgtgaa tgaggtggag gaggacaccg gcaacgagct gtccacaaag
     1201 gagcagatca gccgcaattc caaggccctg gaggagaagt atgtggccga gctgcagctg
     1261 gagcggctga agaaggatgg cgaggtgagg ggctccatca atcgcttcaa gacctctgac
     1321 tacgtgaagg aggccaagca gctgctgaag gtgcagaagg cctaccacca gctggatcag
     1381 tcctttatcg atacatatat cgacctgctg gagacaaggc gcacatacta tgagggacca
     1441 ggagagggct ctcccttcgg ctggaaggac atcaaggagt ggtacgagat gctgatgggc
     1501 cactgcacct attttccaga ggagctgaga agcgtgaagt acgcctataa cgccgatctg
     1561 tacaacgccc tgaatgacct gaacaacctg gtcatcacca gggatgagaa cgagaagctg
     1621 gagtactatg agaagttcca gatcatcgag aacgtgttca agcagaagaa gaagcctaca
     1681 ctgaagcaga tcgccaagga gatcctggtg aacgaggagg acatcaaggg ctaccgcgtg
     1741 acctccacag gcaagccaga gttcaccaat ctgaaggtgt atcacgatat caaggacatc
     1801 acagcccgga aggagatcat cgagaacgcc gagctgctgg atcagatcgc caagatcctg
     1861 accatctatc agagctccga ggacatccag gaggagctga ccaacctgaa tagcgagctg
     1921 acacaggagg agatcgagca gatcagcaat ctgaagggct acaccggcac acacaacctg
     1981 agcctgaagg ccatcaatct gatcctggat gagctgtggc acacaaacga caatcagatc
     2041 gccatcttta accggctgaa gctggtgcca aagaaggtgg acctgtccca gcagaaggag
     2101 atcccaacca cactggtgga cgatttcatc ctgtctcccg tggtgaagcg gagcttcatc
     2161 cagagcatca aagtgatcaa cgccatcatc aagaagtacg gcctgcccaa tgatatcatc
     2221 atcgagctgg ccagggagaa gaactccaag gacgcccaga agatgatcaa tgagatgcag
     2281 aagaggaacc gccagaccaa tgagcggatc gaggagatca tcagaaccac aggcaaggag
     2341 aacgccaagt acctgatcga gaagatcaag ctgcacgata tgcaggaggg caagtgtctg
     2401 tattctctgg aggccatccc tctggaggac ctgctgaaca atccattcaa ctacgaggtg
     2461 gatcacatca tcccccggag cgtgagcttc gacaattctt ttaacaataa ggtgctggtg
     2521 aagcaggagg agaacagcaa gaagggcaat aggacccctt tccagtacct gtctagctcc
     2581 gattctaaga tcagctacga gacattcaag aagcacatcc tgaatctggc caagggcaag
     2641 ggccgcatca gcaagaccaa gaaggagtac ctgctggagg agcgggacat caacagattc
     2701 tccgtgcaga aggacttcat caaccggaat ctggtggaca ccagatacgc cacacgcggc
     2761 ctgatgaatc tgctgcggtc ttatttcaga gtgaacaatc tggatgtgaa ggtgaagagc
     2821 atcaacggcg gcttcacctc ctttctgcgg agaaagtgga agtttaagaa ggagcgcaac
     2881 aagggctata agcaccacgc cgaggatgcc ctgatcatcg ccaatgccga cttcatcttt
     2941 aaggagtgga agaagctgga caaggccaag aaagtgatgg agaacccgaa gaagaaacgt
     3001 aaggtgggta gcagcggtag cccgaaaaag aagcgcaaag tgggcagcga cgcgctggac
     3061 gatttcgatc tggacatgct gggcagcgat gcgctggatg atttcggcgg tggcagctct
     3121 gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca
     3181 cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc
     3241 gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc
     3301 ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacggc
     3361 acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg
     3421 gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac
     3481 taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc
     3541 gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc
     3601 cagagctcca ccgacggcgg tagcggcggt agcggtggta gcggcggcag cggtggtagc
     3661 ggtggcagcg gtggtaacca gatgttcgag gagaagcagg ccgagagcat gcccgagatc
     3721 gagacagagc aggagtacaa ggagattttc atcacacctc accagatcaa gcacatcaag
     3781 gacttcaagg actacaagta ttctcacagg gtggataaga agcccaaccg cgagctgatc
     3841 aatgacaccc tgtatagcac acggaaggac gataagggca ataccctgat cgtgaacaat
     3901 ctgaacggcc tgtacgacaa ggataatgac aagctgaaga agctgatcaa caagtctccc
     3961 gagaagctgc tgatgtacca ccacgatcct cagacatatc agaagctgaa gctgatcatg
     4021 gagcagtacg gcgacgagaa gaacccactg tataagtact atgaggagac aggcaactac
     4081 ctgacaaagt atagcaagaa ggataatggc cccgtgatca agaagatcaa gtactatggc
     4141 aacaagctga atgcccacct ggacatcacc gacgattacc ctaactctcg caataaggtg
     4201 gtgaagctga gcctgaagcc ataccggttc gacgtgtacc tggacaacgg cgtgtataag
     4261 tttgtgacag tgaagaatct ggatgtgatc aagaaggaga actactatga ggtgaacagc
     4321 aagtgctacg aggaggccaa gaagctgaag aagatcagca accaggccga gttcatcgcc
     4381 tctttttaca acaatgacct gatcaagatc aatggcgagc tgtatagagt gatcggcgtg
     4441 aacaatgatc tgctgaacag aatcgaagtg aatatgatcg acatcaccta cagggagtat
     4501 ctggagaaca tgaatgataa gaggccccct cgcatcatca agaccatcgc ctctaagaca
     4561 cagagcatca agaagtacag cacagacatc ctggggaacc tgtatgaagt caagagcaag
     4621 aaacatcctc agattatcaa gaaaggctct ggtggttcta agcgacctgc cgccacaaag
     4681 aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccggc
     4741 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     4801 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     4861 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     4921 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     4981 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     5041 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     5101 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     5161 gaattgctgc cctctggtta tgtgtgggag ggctaagaat tctgcagata tccagcacag
     5221 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     5281 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5341 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5401 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5461 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5521 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5581 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5641 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5701 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5761 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5821 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5881 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5941 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6001 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6061 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6121 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6181 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6241 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6301 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     6361 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6421 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6481 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6541 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6601 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6661 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6721 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6781 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6841 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6901 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6961 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7021 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7081 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7141 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     7201 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     7261 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7321 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7381 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7441 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7501 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7621 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8101 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9661 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     9721 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9781 gtcgctg
//
