LOCUS       sequence-267804-       10993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF13A 361-1749.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF13A tail 361-1749
FEATURES             Location/Qualifiers
     source          1..10993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1309..1311,1312..1314,1315..2022)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1354..1375)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1615..1634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1962..1983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2070..2126
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4792..4801
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6412..6546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6449..6468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6503..6522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6577..6593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6577..6593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6590..6612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6601..6617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6625..6631,6632..6649,6650..6655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6670..6691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6808..6825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6979..7567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7059..7078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7738..8529,8530..8598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8361..8380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8599..8703)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8771..8789
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8827..8849)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8949..8968
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9162..9184
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9176..9193
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9177..9193
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccta ggcctaccat ggtgagcaag
     1321 ggcggggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acggcggagg atccggaggc
     2041 ggctctggcg gagggctgta caagtactca gatctcgagc tcaagcttcg aattctgcag
     2101 tcgacggtac cgcgggcccg ggatcccaat gccaaggtca tccgagagct tcgagaggaa
     2161 gtggagaaac tgagggagca gctctcgcag gctgaggcca tgaaagcccc cgagctaaag
     2221 gagaagctcg aagagtctga gaagctgata aaggagctaa cggtgacgtg ggaagagaag
     2281 ctgaggaaaa cagaagcgat cgcacaggag agacagcggc agctggaaag catgggaatc
     2341 tccctggaga cctctggcat caaggtgggg gacgacaagt gctacctcgt caacctgaac
     2401 gcagaccctg ccctcaacga gcttctggtt tactatttaa aggatcatac ccgggtgggt
     2461 gcagatacct ctcaggacat ccagctcttc ggaataggga ttcagcccga gcactgtgag
     2521 atcgacatcg cagcggatgg agacatcact ctcacgccca aagaaaatgc aaggtcctgt
     2581 ggcaatggca cccttgtctg cagtactacc cagctgtggc acggcgacag gatcttatgg
     2641 ggaaacaacc acttttttag aattaacttg cctaagagga aacggcggga ctggttgaaa
     2701 gactttgaga gagaaaccag ttccgcggag cacgatctag acgctgcgag cgaggcttcc
     2761 tcggagccag actacaacta cgagttcgca cagatggagg tcatcatgaa aacactgaac
     2821 agcaatgatc cagttcaaaa tgtggttcag gtcctggaga agcagtacct ggaagagaag
     2881 cggaccgccc tggaggaaca gcggctcatg tacgaacgcg agctggagca gcttcgccag
     2941 cagctctctc ccgagaggca gccgcccagc agcgcctcag accgtctggc ctacagcagc
     3001 cagacagccc agcagaaggt gacccagtgg gccgaggaga gggatgaact ctttaggcag
     3061 agcttggcca agcttcgaga gcagctggtg aaagccaaca ctttggtgag ggaagccaac
     3121 ttcctagccg aggagatgag caaactcact gactaccaag tgactctgca gatccctgcg
     3181 gccaacctca gtgccaacag gaagagaggc gcaatcgtca gcgagcccgc catccaagcc
     3241 aggaggaaag ggaagggcac ccaggtctgg accattgaga agctggagaa taagctaatt
     3301 gatatgagag acctttacca agaatggaaa gaaaatgttc ctgaggccaa gaggctctat
     3361 gggaagcgag gtgacccttt ttatgaagcg caggagaatc acaacctcat tggtgtggct
     3421 aatgtgtttt tggaatgtct cttctgtgat gtgaagcttc aatatgcagt acccatcatc
     3481 agccagcagg gggaggttgc cgggcgcctc catgtggagg tgacgcgcat tacaggaacc
     3541 attccagaac gcatggcgga ggatgactct tcagagaact ctagtgagag cgggagtctt
     3601 gaagtggtcg acagcagcgg cgaagtcatt caccgggtca aaaagctgac atgcagggtg
     3661 ataattaaag aggcaaccgg gctacccata agcctctcaa atttcgtctt ctgtcagtac
     3721 acgttctggg accagtgtga gtctacagtg gctgctccag tggtggaccc agatgtgccc
     3781 tccccgcagt ccaaggacgc acagtacacg gtgaccttct ctcactgtaa ggactacgtg
     3841 gtgactgtca cagaagagtt cctggagttc atttctgatg gtgcgctggc gattgaagtg
     3901 tggggccaca ggtgtgctgg gaacggaagc cccatctggg aggtggactc gctccatgcg
     3961 aagacaagga cattgcatga caggtggaat gaagtgaccc ggagaataga ggtgtggatc
     4021 tccattctgg agctgaatga gctcggggat tatgccgctg tggagcttca ccaggcgaaa
     4081 gacgtcaaca ccggcggcgt cttccaactc agacagggtc actcccgtag agtgcaggtc
     4141 acagtgaaac ctgtacagca ctcagggaca ctgccactca tggttgaagc catcctatca
     4201 gtatccattg gctgcgtgac cgccaggtcc accaagctgc agagagggct ggacagctac
     4261 caggaagaag acttgaattg tgtaagagag agatggtccg atgcactcat taaacgacgg
     4321 gaatacttag acgaacagat taaaaaagtc agcaataaaa aagagaaaac agaagacgac
     4381 atggagcggg aagccaggct ggtggagcag tgggtggggc tgacagagga gaggaatgct
     4441 gtgctggtgc ccgcccctgg cagcggaatc cccggggcac ctgctgactg ggtcccacct
     4501 cctggcatgg aaacacacat cccggttctc ttcctggatc tgaacgcgga tgacctcagt
     4561 gccaatgagc agctggtggg cccccatgcg tccggtgtga actccatcct gcccaaggaa
     4621 cacggcagtc agtttttcta cctgcccatc ataaagcaca gtgatgacga ggtttcagcc
     4681 acagcctcct gggactcctc tgtgcacgac tctcttcacc tgaatcgggt cacaccgcag
     4741 aatgaaagga tctacctgat cgtgaagacc accgtgcagc tcagccaccc ggccgccatg
     4801 gagctagtgt tgcggaagcg gatcgcggcc aatatttata acaagcagag tttcacccag
     4861 agtttgaaga gacgaatatc actgatcaat atacgttact cctgcggcgt gacctatgag
     4921 atagtatcca atatcccaaa ggcgaccgag gagattgagg accgggaaac cctggccctc
     4981 ctggcagcgc ggagtgagaa cgagggcact ttggatgggg agacatacat cgagaagtac
     5041 acgcggggcg tgctgcaggt ggagaatatc ctgagcctgg agcggctccg gcaggctgtc
     5101 acggtgaagg aagcgctttc caccaaagcg aggcacattc ggagaagcct cagtacaccg
     5161 aacgttcaca acgtctcctc tagccggcca gatctctctg gcttcgatga agacgataag
     5221 ggctggcccg agaaccagtt agacgtgtct gactacagct ccagctacca agacgtcgcg
     5281 tgctatggga ctttacccag ggactcacct cgcaggagca aggaaggttg tccatcagag
     5341 aatcctcatg ccttaacagt cagccctttt aaagcgttct ctcctcagcc accaaagttc
     5401 ttcaaacccc tcatgcctgt aaaagaggag cataagaaaa ggttggccct ggaagccaga
     5461 cctctcctaa gccaagagga ctcggaagag gaggaaaacg agctggaagc tctcagcagg
     5521 aagctgatgc ttacacagcc ttatgtgccc gtggagttcg ctgacttcag tgtttacaat
     5581 gccagcttgg agaacaggga gtggtcctct tctaaggcgg acctgacaga ctcgagagcc
     5641 ttggagaaag cagtgtcccg tagccctacc accagcagcc tcaccagtgg ctacttctcc
     5701 cacagtgcct ccaatgccac tctgtctgac atggcagtcc cctccagcga cagctcagac
     5761 cagcttgccg tctcgacaaa ggaggtcgag tgcgccgagc ccccgggacc atcacttgca
     5821 ccggatgtca gacgagcttc gaatcaggag cttacagaag taggaagagg ctcagggaag
     5881 gatgagacaa tcgctgtgcc gctggaggaa aacagtgcct tacccaaggg gaccccgtca
     5941 ccccagagca tccctgagga aagttccaga atgccatgca ggactgcttc atgttcagaa
     6001 ctggatgtcg gccccagcaa agatggccac caagccagag aattctgccc aggagaggtg
     6061 acgatagaac acactacaaa cattttggag gaccattcct tcacagagtt catgggtgtt
     6121 tctgatggca aagactttga tggtttggca gattgttctg taggggagcc ctccaggagg
     6181 agggctctaa caaacgaaac agaccataaa ggtatcccag aaagacctcc agatgccgac
     6241 cggctgcatc ccaagataga gaacgaccag gaagccacag ccactcggta agctagataa
     6301 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
     6361 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6541 tggatccccg ggtaccgagc tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     6601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8701 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8761 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     8821 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8881 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     8941 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     9001 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     9061 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9121 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     9181 aacgacggcc agtgccaagt tgggatcttt gcattggccc acggctctca ggatggggat
     9241 gctccccttc agcacccggt tccccttgga aactgatggt cctggctctg tggcatggca
     9301 gtggcactgt gaggagcccc ctaccagcag cacacagtgg ggttggcact gccacgctcc
     9361 ggatgccgcg ctctgatcca accccataat caagggaacc cgaattgccc catcattgcc
     9421 cccaccaccc ccatcctgcc gggccctcac accccacgct gccttctggt gacattcccc
     9481 agcccaaacc cacggcttca tggctaccgc ggggcatttc ccattgccgc cccattatca
     9541 gctctgcaca cctcccgctg tacccatgcc tcgtggctgc ccttctttga cgtataatct
     9601 tctaattaat acccggcctt gtcaaagtgg agcacaaacg ttaattaatt ccccagcagg
     9661 caggtaatta acagtgtgac tccctttttg ctgcgagtgg ggctgataca gagagatgtg
     9721 gcactatgga gcccacgggg tcctggcact gggtgcccac ggaggtcccc atgtgctgca
     9781 gtgtcaccgc ctccgaggag acagtattgt ccctgcggtg tccctgcagc tcagctctgt
     9841 ccacagggcc acctccagtt tggaggggac acaatgcagc cccgatgcaa cccatcctcg
     9901 cagcatccca gggacaaaga ccccactgca agaccgcaca cagggctggg tcccgctccc
     9961 ctaatatcta cagtgcttct tgcatggccc cttaatcaat gcagttaatc agcatgcgct
    10021 catgcaccgc tctggagctg caaagcccct cgcagcgctg ctcaccaaca ccgcgcaccg
    10081 ccccggccca gcctgcagca cgcgctgcaa acaggaaaga aacaaaatat tgcccaaatg
    10141 taggcaaagg cattcggctg ccttgacctc cgccgggccg ggccctgcct gactcagctc
    10201 cttactcagc gctcgcttcc tccctccggc tgccaccgcc gcagcgcaca ccctgacaaa
    10261 gagtggccct taacgggctc tgaggtgcac ccagcagtgc actcagcagt ccaagggccg
    10321 gcctggaggt ttgcaccgct acgtgctgac attagcattg aacttggccc tgggtagtgc
    10381 tgcaggccgg gcggggtggg tgtagagagt gcagcgcgcg ttgcacccgg tgccccttcc
    10441 cctcccttgc atcccagcag gctgcacccc agcaccaggc ccgtgcatgc atgctcctgg
    10501 tgttattgca gcctggtgca tgcatgcgtc ttgatggtgc agcgctgtgc atgcatcctc
    10561 cttggtgtgt agcagtctag tgcatgcata cccctcggtg ttattgctgc tctgtgcacg
    10621 cacgctcatt gtatcacttc atcccagtgc atgcactcac actggagcga ttgctgctcg
    10681 gtgcacgcac actcattgta tcactgcagc tcagtggctg cacgcacacc ggtgttattg
    10741 ctgctcggtg cgtgcatgca catcagtgtc gctgcagctc agtgcatgca cgctcattgc
    10801 ccatcgctat ccctgcctct cctgctggcg ctccccggga ggtgacttca aggggaccgc
    10861 aggaccacct cgggggtggg gggagggctg cacacgcgga ccccgctccc cctccccaac
    10921 aaagcactgt ggaatcaaaa aggggggagg ggggatggag gggcgcgtca cacccccgcc
    10981 ccacaccctc acc
//
