LOCUS       sequence-267805-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (L132 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 L132 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        159..538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..742
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     693..713
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     787..806
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        787..805
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1227..1247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1263..1283
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4695..4742
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4743..4766
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4776..4832
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4833..5231
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5302..5319)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5308..5532
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5578..6006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5665..5684)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5875..5896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6015..6035)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6020..6349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6200..6335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6262..6281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6416..7210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6470..6489)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7080..7099
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7384..7505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7421..7440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7475..7494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7554..7570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7554..7570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7567..7589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7578..7594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7602..7608,7609..7626,7627..7632))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7647..7668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7785..7802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7956..8544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8036..8055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8715..9506,9507..9575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9338..9357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9576..9680)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9755..9774)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
       61 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      121 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      601 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      661 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      721 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      781 cgaaattaat acgactcact atagggagac ccaagctggc tagcgccacc atgaagcgaa
      841 attacattct ggggctggcc attggcatta catcagtggg ctatggcatc attgactacg
      901 agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac gtggagaaca
      961 atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg cgccacagaa
     1021 tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac tccgagctgt
     1081 ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg tctgaggagg
     1141 agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac gtgaatgagg
     1201 tggaggagga caccggcaac gagctgccga agaagaaacg taaggtgggt agcagcggta
     1261 gcccgaaaaa gaagcgcaaa gtgggcagcg acgcgctgga cgatttcgat ctggacatgc
     1321 tgggcagcga tgcgctggat gatttcggcg gtggcagctc tgaggtggag ttttcccacg
     1381 agtactggat gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc
     1441 ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca
     1501 tcggcctgca cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg
     1561 tcatgcagaa ctacagactg attgacgcca ccctgtacgg cacattcgag ccttgcgtga
     1621 tgtgcgccgg cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaacg
     1681 caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc
     1741 gcgtcgaaat taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct
     1801 ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc ccagagctcc accgacggcg
     1861 gtagcggcgg tagcggtggt agcggcggca gcggtggtag cggtggcagc ggtggtctgt
     1921 ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat gtggccgagc
     1981 tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat cgcttcaaga
     2041 cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc taccaccagc
     2101 tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc acatactatg
     2161 agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg tacgagatgc
     2221 tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac gcctataacg
     2281 ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg gatgagaacg
     2341 agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag cagaagaaga
     2401 agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac atcaagggct
     2461 accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat cacgatatca
     2521 aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat cagatcgcca
     2581 agatcctgac catctatcag agctccgagg acatccagga ggagctgacc aacctgaata
     2641 gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac accggcacac
     2701 acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac acaaacgaca
     2761 atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac ctgtcccagc
     2821 agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg gtgaagcgga
     2881 gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc ctgcccaatg
     2941 atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag atgatcaatg
     3001 agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc agaaccacag
     3061 gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg caggagggca
     3121 agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat ccattcaact
     3181 acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt aacaataagg
     3241 tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc cagtacctgt
     3301 ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg aatctggcca
     3361 agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag cgggacatca
     3421 acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc agatacgcca
     3481 cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg gatgtgaagg
     3541 tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag tttaagaagg
     3601 agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc aatgccgact
     3661 tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag aaccagatgt
     3721 tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag tacaaggaga
     3781 ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac aagtattctc
     3841 acagggtgga taagaagccc aaccgcgagc tgatcaatga caccctgtat agcacacgga
     3901 aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac gacaaggata
     3961 atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg taccaccacg
     4021 atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac gagaagaacc
     4081 cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc aagaaggata
     4141 atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc cacctggaca
     4201 tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg aagccatacc
     4261 ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag aatctggatg
     4321 tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag gccaagaagc
     4381 tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaacaat gacctgatca
     4441 agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg aacagaatcg
     4501 aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat gataagaggc
     4561 cccctcgcat catcaagacc atcgcctcta agacacagag catcaagaag tacagcacag
     4621 acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt atcaagaaag
     4681 gctctggtgg ttctaagcga cctgccgcca caaagaaggc tggacaggct aagaagaaga
     4741 aagattacaa agacgatgac gataagggat ccggcgcaac aaacttctct ctgctgaaac
     4801 aagccggaga tgtcgaagag aatcctggac cgatggccaa gcctttgtct caagaagaat
     4861 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     4921 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     4981 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     5041 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     5101 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     5161 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     5221 gggagggcta agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     5281 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5341 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5401 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5461 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5521 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5581 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5641 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5701 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5761 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5821 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5881 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5941 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6001 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6061 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6301 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6361 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6421 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6481 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6541 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6601 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6661 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6721 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6781 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6841 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6901 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6961 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7021 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7081 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7141 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7201 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7261 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7321 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7381 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7441 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7501 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7561 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7621 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7681 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7741 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7801 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7861 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7921 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7981 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8041 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8101 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8161 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8221 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8281 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8341 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8401 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8461 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8521 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8581 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8641 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8701 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8761 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8821 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8881 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8941 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9001 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9061 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9121 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9181 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9241 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9301 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9361 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9421 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9481 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9541 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9601 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9661 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9721 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9781 taagcca
//
