LOCUS       sequence-267806-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (N130 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 N130 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        180..559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        560..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     714..734
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     808..827
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        808..826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1242..1262
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1278..1298
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4716..4763
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4764..4787
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4797..4853
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4854..5252
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5323..5340)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5329..5553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5599..6027
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5686..5705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5896..5917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6036..6056)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6041..6370
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6221..6356
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6283..6302
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6437..7231
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6491..6510)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7101..7120
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7405..7526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7442..7461)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7496..7515
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7575..7591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7575..7591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7588..7610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7599..7615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7623..7629,7630..7647,7648..7653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7668..7689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7806..7823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7977..8565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8057..8076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8736..9527,9528..9596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9359..9378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9597..9701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9776..9787,1..8))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
       61 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      121 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      181 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      241 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      301 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      361 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      421 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      481 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      541 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      601 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      661 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      721 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      781 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      841 ctagcgccac catgaagcga aattacattc tggggctggc cattggcatt acatcagtgg
      901 gctatggcat cattgactac gagacaaggg acgtgatcga cgccggcgtg agactgttca
      961 aggaggccaa cgtggagaac aatgagggcc ggagatccaa gaggggagca aggcgcctga
     1021 agcggagaag gcgccacaga atccagagag tgaagaagct gctgttcgat tacaacctgc
     1081 tgaccgacca ctccgagctg tctggcatca atccttatga ggccagagtg aagggcctgt
     1141 cccagaagct gtctgaggag gagtttagcg ccgccctgct gcacctggca aagaggagag
     1201 gcgtgcacaa cgtgaatgag gtggaggagg acaccggcaa cccgaagaag aaacgtaagg
     1261 tgggtagcag cggtagcccg aaaaagaagc gcaaagtggg cagcgacgcg ctggacgatt
     1321 tcgatctgga catgctgggc agcgatgcgc tggatgattt cggcggtggc agctctgagg
     1381 tggagttttc ccacgagtac tggatgagac atgccctgac cctggccaag agggcacgcg
     1441 atgagaggga ggtgcctgtg ggagccgtgc tggtgctgaa caatagagtg atcggcgagg
     1501 gctggaacag agccatcggc ctgcacgacc caacagccca tgccgaaatt atggccctga
     1561 gacagggcgg cctggtcatg cagaactaca gactgattga cgccaccctg tacggcacat
     1621 tcgagccttg cgtgatgtgc gccggcgcca tgatccactc taggatcggc cgcgtggtgt
     1681 ttggcgtgag gaacgcaaaa accggcgccg caggctccct gatggacgtg ctgcactacc
     1741 ccggcatgaa tcaccgcgtc gaaattaccg agggaatcct ggcagatgaa tgtgccgccc
     1801 tgctgtgcta tttctttcgg atgcctagac aggtgttcaa tgctcagaag aaggcccaga
     1861 gctccaccga cggcggtagc ggcggtagcg gtggtagcgg cggcagcggt ggtagcggtg
     1921 gcagcggtgg taacgagctg tccacaaagg agcagatcag ccgcaattcc aaggccctgg
     1981 aggagaagta tgtggccgag ctgcagctgg agcggctgaa gaaggatggc gaggtgaggg
     2041 gctccatcaa tcgcttcaag acctctgact acgtgaagga ggccaagcag ctgctgaagg
     2101 tgcagaaggc ctaccaccag ctggatcagt cctttatcga tacatatatc gacctgctgg
     2161 agacaaggcg cacatactat gagggaccag gagagggctc tcccttcggc tggaaggaca
     2221 tcaaggagtg gtacgagatg ctgatgggcc actgcaccta ttttccagag gagctgagaa
     2281 gcgtgaagta cgcctataac gccgatctgt acaacgccct gaatgacctg aacaacctgg
     2341 tcatcaccag ggatgagaac gagaagctgg agtactatga gaagttccag atcatcgaga
     2401 acgtgttcaa gcagaagaag aagcctacac tgaagcagat cgccaaggag atcctggtga
     2461 acgaggagga catcaagggc taccgcgtga cctccacagg caagccagag ttcaccaatc
     2521 tgaaggtgta tcacgatatc aaggacatca cagcccggaa ggagatcatc gagaacgccg
     2581 agctgctgga tcagatcgcc aagatcctga ccatctatca gagctccgag gacatccagg
     2641 aggagctgac caacctgaat agcgagctga cacaggagga gatcgagcag atcagcaatc
     2701 tgaagggcta caccggcaca cacaacctga gcctgaaggc catcaatctg atcctggatg
     2761 agctgtggca cacaaacgac aatcagatcg ccatctttaa ccggctgaag ctggtgccaa
     2821 agaaggtgga cctgtcccag cagaaggaga tcccaaccac actggtggac gatttcatcc
     2881 tgtctcccgt ggtgaagcgg agcttcatcc agagcatcaa agtgatcaac gccatcatca
     2941 agaagtacgg cctgcccaat gatatcatca tcgagctggc cagggagaag aactccaagg
     3001 acgcccagaa gatgatcaat gagatgcaga agaggaaccg ccagaccaat gagcggatcg
     3061 aggagatcat cagaaccaca ggcaaggaga acgccaagta cctgatcgag aagatcaagc
     3121 tgcacgatat gcaggagggc aagtgtctgt attctctgga ggccatccct ctggaggacc
     3181 tgctgaacaa tccattcaac tacgaggtgg atcacatcat cccccggagc gtgagcttcg
     3241 acaattcttt taacaataag gtgctggtga agcaggagga gaacagcaag aagggcaata
     3301 ggaccccttt ccagtacctg tctagctccg attctaagat cagctacgag acattcaaga
     3361 agcacatcct gaatctggcc aagggcaagg gccgcatcag caagaccaag aaggagtacc
     3421 tgctggagga gcgggacatc aacagattct ccgtgcagaa ggacttcatc aaccggaatc
     3481 tggtggacac cagatacgcc acacgcggcc tgatgaatct gctgcggtct tatttcagag
     3541 tgaacaatct ggatgtgaag gtgaagagca tcaacggcgg cttcacctcc tttctgcgga
     3601 gaaagtggaa gtttaagaag gagcgcaaca agggctataa gcaccacgcc gaggatgccc
     3661 tgatcatcgc caatgccgac ttcatcttta aggagtggaa gaagctggac aaggccaaga
     3721 aagtgatgga gaaccagatg ttcgaggaga agcaggccga gagcatgccc gagatcgaga
     3781 cagagcagga gtacaaggag attttcatca cacctcacca gatcaagcac atcaaggact
     3841 tcaaggacta caagtattct cacagggtgg ataagaagcc caaccgcgag ctgatcaatg
     3901 acaccctgta tagcacacgg aaggacgata agggcaatac cctgatcgtg aacaatctga
     3961 acggcctgta cgacaaggat aatgacaagc tgaagaagct gatcaacaag tctcccgaga
     4021 agctgctgat gtaccaccac gatcctcaga catatcagaa gctgaagctg atcatggagc
     4081 agtacggcga cgagaagaac ccactgtata agtactatga ggagacaggc aactacctga
     4141 caaagtatag caagaaggat aatggccccg tgatcaagaa gatcaagtac tatggcaaca
     4201 agctgaatgc ccacctggac atcaccgacg attaccctaa ctctcgcaat aaggtggtga
     4261 agctgagcct gaagccatac cggttcgacg tgtacctgga caacggcgtg tataagtttg
     4321 tgacagtgaa gaatctggat gtgatcaaga aggagaacta ctatgaggtg aacagcaagt
     4381 gctacgagga ggccaagaag ctgaagaaga tcagcaacca ggccgagttc atcgcctctt
     4441 tttacaacaa tgacctgatc aagatcaatg gcgagctgta tagagtgatc ggcgtgaaca
     4501 atgatctgct gaacagaatc gaagtgaata tgatcgacat cacctacagg gagtatctgg
     4561 agaacatgaa tgataagagg ccccctcgca tcatcaagac catcgcctct aagacacaga
     4621 gcatcaagaa gtacagcaca gacatcctgg ggaacctgta tgaagtcaag agcaagaaac
     4681 atcctcagat tatcaagaaa ggctctggtg gttctaagcg acctgccgcc acaaagaagg
     4741 ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga tccggcgcaa
     4801 caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggcca
     4861 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     4921 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     4981 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     5041 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     5101 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     5161 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     5221 tgctgccctc tggttatgtg tgggagggct aagaattctg cagatatcca gcacagtggc
     5281 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     5341 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5401 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5461 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5521 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     5581 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5641 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5701 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5761 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5821 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5881 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5941 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6001 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6061 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6121 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6181 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6241 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6301 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6361 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     6421 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6481 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6541 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6601 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6661 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6721 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6781 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6841 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     6901 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6961 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7021 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7081 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7141 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7201 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     7261 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7321 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7381 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7441 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7501 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7561 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7621 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7681 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7741 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7801 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7861 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7921 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7981 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8461 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8521 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8581 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8641 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8761 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8821 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8881 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8941 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9061 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9121 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9181 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9241 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9361 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9421 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9481 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9541 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9661 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9721 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     9781 tctgctc
//
