LOCUS       sequence-267814-       16504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS 
            for cloning gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16504)
  AUTHORS   Worthen JM, Yamburenko MV, Lim J, Nimchuk ZL, Kieber JJ, Schaller GE
  TITLE     Type-B response regulators of rice play key roles in growth, 
            development, and cytokinin signaling.
  JOURNAL   Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870.
  PUBMED    31160418
REFERENCE   2  (bases 1 to 16504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870."
COMMENT     Sequence Label: pARS3_MUbCAS9_MC
FEATURES             Location/Qualifiers
     source          1..16504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    88..112
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     132..154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3692..3716
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3735..3790
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3798..7901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3798..7901
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             5584..5592
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7902..7928
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8195..8219)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      8237..8944
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(8977..8995)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8978..8995)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        9088..10796
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          9835..10796
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     CDS             11031..12056
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12075..12249
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12362..12386
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      12466..13097
                     /label=oriV
                     /note="incP origin of replication"
     CDS             13309..14103
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             14402..15550
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(15619..15636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15790..16378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15870..15889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
       61 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
      121 tagtttcgcg tagcggataa caatttcaca cagggatatc actagtaaaa ggtacattta
      181 aatcagttcg atgaccctag gctgaactgg tacggccggc ctcgatgctt acgcacgtga
      241 attccggatc gcctgcaggg acttgacaat tgtaccagtt taaacaagtt gtaatgagtt
      301 gctggcctct cttgggattg ttcatcaaag tagctgtccc tacacaccac aacagaatgg
      361 tgttgtggag agaaagcaca gacacatcct tgagatggca agggcactta agtttcagag
      421 tggtgtacct accaggtttt ggggagactg tgtcaagact gttgtatacc tgatcaatag
      481 actccctact ccaatcttac aaggcaaatg tccatatgaa ctactctatc agaaacatgc
      541 caagcttgat cacttgagag tgtttgggtg tcttgctttt gcagcaacac tgcccaaagg
      601 tgacaaactt gcacctagag ccaagcgaac catcttcatt ggttattctg agactcaaaa
      661 gggttacagg ttgtatgact tggataataa ggtgatcata gtgagcaggg atgtagtttt
      721 cagagagttt cagttccctt tcaaagagtc catctcccat gaacctgata tgttcactcg
      781 agcaggttca cttagctctg aagatacagc tttgcagcta gtgtataatg atatttttcc
      841 ttccagcttt catactgatg atgatgatac tccagttctt attcctgaca ttgtagaggc
      901 caatgaggat atcactttgg ggactcattc tacccaaaat catatcactt cagctgatgc
      961 tgcaccttca ggccacctgc atgctcctgc agagtcagtt gagtcagctg agcctgacct
     1021 agcaaatgct gaatttgagc accatactgc tgtagctgat ccctctctag ttccagcaaa
     1081 cctgcacact agacctaaac gcaatgcagg tcctcccatc tggctcaagg actttgtgac
     1141 actgaacaaa ggctctaggg atgttccata ccctatagcc aactatgttt cctatgatca
     1201 cttgcctgtt cactatcaag cttatttgag tgctttctcc acttatactg aacctaaatc
     1261 cttcaaagaa gcagctcaag atgagaaatg gatggaggcc atgtcccttg agatacaggc
     1321 tcttgaggat aataacacct gggagattgt ccctttaccc cctggtaaac agcctatagg
     1381 gtccaaatgg gtgtacaaaa ttaaatacaa agctaatggt gaagttgaca ggtttaaggc
     1441 aaggctagtg gccaagggat acactcagca agaaggcctt gactaccatg aaactttttc
     1501 tccagtggcc aaaatggtca ctgtaaaagc tgtcatatct gttgctgctt ccaagggctg
     1561 gttccttttc cagatggatg tcaacaatgc ttttctacaa ggtgacctca tggaagaagt
     1621 ttatatgtct ctgcctcagg gttttcacag ccaaggggag accaaagtgt gcaggctgtt
     1681 gaatccctct atggtttaaa gcaagcatca aggcagtgga acatcaagct taccactgtc
     1741 cttatgcagg ctggttttat gcaaagtgct tatgatcact ccttgtttac caaaagagag
     1801 gggactgacc ttgtcataat cctgatttat gtggatgatc tgttaataac aggcagcagc
     1861 aatgttttga tttcagaagc aaaggcaacc ctgcatcagc atttcaagat gaaagatttg
     1921 ggagaactaa aatacttctt aggcattgag gtgctgagat cagaaaaggg aatcctactg
     1981 aaccagagga agtatgcact ggaactgata tcaggtgttg gtctgggagg ctgcagacca
     2041 gtgtcaaccc caatggaaca gaaccaaagg ctgactactg tagaatatga caagcactta
     2101 ggaaagactg atgatgcaga gttagaggat gtgggttcct atcagagact ggtgggaaag
     2161 cttctctact tgacaatcac aaggccagat atcagctttg cagtgcaggt gctgagtcaa
     2221 ttcatgcagc aacccaaaca gtcacacctg gaagcagcat tgagggttgt gaagtacata
     2281 aaaggttctc caggtgttag gaagcggccg ctgcagcgtc gcgtcgggcc aagcgaagca
     2341 gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt
     2401 ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc
     2461 acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca
     2521 ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct
     2581 ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc
     2641 cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc cctctctacc
     2701 ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt
     2761 ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac
     2821 ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg
     2881 gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat
     2941 agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc
     3001 atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc
     3061 tagatcggag tagaaatctg tttcaaacta cctggtggat ttattaattt tggatctgta
     3121 tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa atatcgatct
     3181 aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt
     3241 cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta
     3301 gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat
     3361 acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat
     3421 gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc
     3481 tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt attttgatct
     3541 tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt
     3601 catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt
     3661 gttacttctg cagggcgcgc cgatatcatc aacaagtttg tacaaaaaag caggctcttt
     3721 aaagtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta caattactat
     3781 ttacaattac aaaaaaaatg gacaaaaaat acagcattgg gttggacatt gggacaaata
     3841 gcgtgggttg ggcagtcatt acagacgaat acaaggttcc tagcaagaag ttcaaggtgt
     3901 tgggcaacac tgacaggcac tcgatcaaga aaaatctcat tggtgcactc cttttcgata
     3961 gtggggagac tgccgaagcg accagactga aaaggactgc tagaaggcgc tatacccggc
     4021 gtaagaatcg catttgctac ctccaggaga tattctctaa cgaaatggcc aaggttgatg
     4081 acagcttctt tcatcgcttg gaggaatcat tcctcgtcga ggaagacaag aaacacgagc
     4141 ggcatccgat ctttggaaac attgttgatg aggtcgcata tcacgaaaag taccccacga
     4201 tctaccatct tcgcaagaaa ctggtggatt caactgacaa ggctgatctg cggttgatat
     4261 acctcgcact tgctcacatg atcaagttcc gtggccattt tcttattgag ggagacctga
     4321 accctgataa ttctgacgtc gataagttgt tcatccagct cgtgcaaaca tacaatcagc
     4381 tctttgagga aaaccctatc aacgcctctg gagttgatgc caaagcgatc ctttccgcga
     4441 ggctgtcgaa gagtagaagg ctggagaact tgatcgccca acttcctggc gaaaagaaaa
     4501 acggtttgtt cgggaatctc attgcgctga gcttgggact cacgccaaac ttcaagtcta
     4561 acttcgacct cgcagaggat gctaaacttc agctgtccaa ggatacttat gatgacgatc
     4621 ttgacaacct gttggcacaa atcggtgacc agtacgctga tttgttcctc gctgccaaga
     4681 acctcagcga tgccatactc ctttcagaca tcctccgcgt gaacactgag attaccaaag
     4741 cacctctgtc tgcttccatg ataaagcgtt atgatgagca ccatcaagac ctcactctgt
     4801 tgaaggcgtt ggttagacag caactcccag agaagtacaa agaaatcttc tttgaccaat
     4861 ctaagaatgg ctacgccgga tatattgatg gcggagcgtc ccaggaggag ttctacaagt
     4921 ttatcaaacc tattcttgag aagatggatg gtacggagga actccttgtt aaactgaata
     4981 gagaggatct cttgcgcaag caacggactt tcgacaacgg cagcataccg caccagatcc
     5041 atctcggaga gcttcacgcc atccttcgcc ggcaggaaga tttctacccc tttctgaagg
     5101 acaaccgtga gaagatcgaa aaaatcctta ctttcagaat cccttactat gtcggtccac
     5161 tggctagagg caactctaga tttgcttgga tgacacgcaa gagtgaggaa actatcaccc
     5221 cttggaactt cgaggaagtg gttgacaagg gcgcatcggc tcaaagtttc attgagagaa
     5281 tgactaattt tgataagaac cttccgaacg agaaagtcct gcccaagcat tcactccttt
     5341 acgagtattt cacagtgtat aacgaactta cgaaggtgaa atacgttact gagggtatgc
     5401 ggaagccggc attcctgtcg ggggaacaaa agaaagctat agtggatctg ttgttcaaaa
     5461 ctaacaggaa ggttaccgtc aagcagctta aggaggatta cttcaagaaa atagaatgtt
     5521 ttgactcggt ggagatcagt ggcgttgaag atagattcaa cgcttcactg ggaacctacc
     5581 acgacctcct taagatcatt aaggacaaag atttcttgga taacgaggaa aatgaggaca
     5641 ttctcgaaga tatagtcctt acactgacgt tgtttgagga ccgtgaaatg atcgaggaaa
     5701 gattgaagac ctatgctcat ctcttcgacg ataaggttat gaagcagctc aagcgtagaa
     5761 ggtacacagg ttgggggcgt ctttctagaa agctgattaa tggcataagg gataaacaat
     5821 ctggaaagac gattctggat ttcctcaagt ccgacggctt cgccaaccgc aattttatgc
     5881 agcttataca cgacgattcc ctgactttca aagaggacat ccagaaggcc caagttagcg
     5941 gccaaggaga ttcacttcac gagcatatcg ccaacctggc gggtagcccg gcgatcaaga
     6001 aagggattct tcagaccgtc aaggtcgtgg atgagctggt gaaagttatg ggccggcata
     6061 agcccgaaaa catagtgatc gagatggccc gtgaaaatca gactacccaa aaaggacaga
     6121 agaactcccg cgagcggatg aaaaggatag aggaaggcat caaggaactt ggaagccaga
     6181 tcctgaagga gcacccagtt gaaaacacac agttgcaaaa tgagaagctc tatctttact
     6241 atctccaaaa tggtagagac atgtatgtgg atcaggagtt ggacattaac aggctctcag
     6301 actacgatgt cgaccatatc gtgccgcaat cgttccttaa ggacgatagt atagataata
     6361 aggtgctgac tcggtcggat aaaaaccgtg gcaagtcgga caatgttccc agtgaggaag
     6421 ttgtgaagaa gatgaagaac tactggcgcc aactgttgaa tgcaaaattg atcacccaga
     6481 ggaagtttga caacctcaca aaagcagagc gcggtgggct cagtgaactt gataaagctg
     6541 gattcattaa gaggcaactt gttgagacac gccagattac gaagcacgtc gcgcagatac
     6601 tggattctag gatgaacacc aagtacgacg agaatgataa attgatcaga gaagtgaagg
     6661 ttatcacatt gaagtctaaa ctcgtgtccg attttcgcaa agacttccaa ttttataagg
     6721 ttcgggagat caacaattat caccatgcac atgatgctta cctcaacgcc gtggttggta
     6781 cggcgttgat taagaaatac ccgaagctgg agtctgagtt cgtgtacggg gattataagg
     6841 tctacgacgt gaggaaaatg atcgctaaga gtgagcagga aattggtaaa gccaccgcga
     6901 agtatttctt ttactctaac atcatgaact tcttcaagac agagataacg ttggcaaatg
     6961 gtgaaatacg taagagacct ctcatcgaga ctaacggtga aaccggggaa atcgtttggg
     7021 ataaagggcg ggactttgct actgttcgta aggtcctctc catgccgcaa gtgaatattg
     7081 ttaagaaaac agaggtccag acgggcggat tctctaagga atccatcctt cccaaaagaa
     7141 acagcgacaa gctgattgcg aggaagaaag attgggaccc taagaaatat ggtgggttcg
     7201 attctccaac cgttgcctac tccgtcttgg tcgtggcgaa ggttgagaag ggtaaatcta
     7261 agaaactcaa atccgtcaag gaactccttg ggattactat catggagagg tcttccttcg
     7321 aaaagaatcc tatcgatttc cttgaggcta aaggctataa ggaagtgaag aaggatctca
     7381 tcatcaagct cccaaagtac agcctgtttg agttggaaaa cggaaggaag cgcatgttgg
     7441 catcagctgg agagctccag aaagggaacg agctggcctt gccttctaag tatgtgaact
     7501 tcctctatct tgcgtcgcac tacgagaagc tgaaaggcag tccagaggac aacgaacaga
     7561 aacaactttt tgttgagcaa cacaagcatt atctggatga gattatagaa cagatcagcg
     7621 agttctcaaa aagagtcatt cttgccgatg ctaacctcga caaggtgctc tccgcataca
     7681 acaaacacag agacaagccg atcagggagc aggctgaaaa tatcattcat ctcttcactc
     7741 ttaccaacct gggtgccccc gcggcattca agtattttga tacaacgatt gaccggaaac
     7801 gttacacaag cacgaaggag gtccttgatg cgaccctgat tcatcaaagc ataacagggc
     7861 tctatgagac gagaatcgat ttgtcacagc tcggcggaga ctacccgtat gacgtgcccg
     7921 attacgctgc cgcggcagct gagttcaagc gcgaggaaca ggcccggaag gcgaaagtga
     7981 acaatgagaa gaaaacagaa atcgttaaac ctgagagctg ctcaaacgag ggagacgtta
     8041 aggatcttaa gaggaaagac tctgaggatg gcaacgaagg agaggaagag gaagctagct
     8101 caaaacctaa gaaaccaaag gtggcactct cacatctcca agacattgac gacaccgaag
     8161 cagaccaaga agaagaataa ctcgagatat ctagacccag ctttcttgta caaagtggtt
     8221 gatgatatct agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
     8281 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
     8341 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
     8401 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
     8461 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
     8521 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
     8581 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
     8641 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
     8701 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
     8761 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
     8821 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
     8881 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
     8941 gcaggaccgg catgcaagct agcttactag tgatattcta tagtgtcacc taaatctgcg
     9001 gccgcaaagt cgacgaattc tcccgggtgg atccaaggcc ttctagaaaa actcgagaac
     9061 gttgggcccc aattggctag caagcttatt cgggtcaagg ggaagccagc gcgccacccc
     9121 acgtcagcaa atacggaggc gcggggttga cggcgtcacc cggtcctaac ggcgaccaac
     9181 aaaccagcca gaagaaatta cagtaaaaaa aaagtaaatt gcactttgat ccacctttta
     9241 ttacctaagt ctcaatttgg atcaccctta aacctatctt ttcaatttgg gccgggttgt
     9301 ggtttggact accatgaaca acttttcgtc atgtctaact tccctttcag caaacatatg
     9361 aaccatatat agaggagatc ggccgtatac tagagctgat gtgtttaagg tcgttgattg
     9421 cacgagaaaa aaaaatccaa atcgcaacaa tagcaaattt atctggttca aagtgaaaag
     9481 atatgtttaa aggtagtcca aagtaaaact tatagataat aaaatgtggt ccaaagcgta
     9541 attcactcaa aaaaaatcaa cgagacgtgt accaaacgga gacaaacggc atcttctcga
     9601 aatttcccaa ccgctcgctc gcccgcctcg tcttcccgga aaccgcggtg gtttcagcgt
     9661 ggcggattct ccaagcagac ggagacgtca cggcacggga ctcctcccac cacccaaccg
     9721 ccataaatac cagccccctc atctcctctc ctcgcatcag ctccaccccc gaaaaatttc
     9781 tccccaatct cgcgaggctc tcgtcgtcga atcgaatcct ctcgcgtcct caaggtacgc
     9841 tgcttctcct ctcctcgctt cgtttcgatt cgatttcgga cgggtgaggt tgttttgttg
     9901 ctagatccga ttggtggtta gggttgtcga tgtgattatc gtgagatgtt taggggttgt
     9961 agatctgatg gttgtgattt gggcacggtt ggttcgatag gtggaatcgt ggttaggttt
    10021 tgggattgga tgttggttct gatgattggg gggaattttt acggttagat gaattgttgg
    10081 atgattcgat tggggaaatc ggtgtagatc tgttggggaa ttgtggaact agtcatgcct
    10141 gagtgattgg tgcgatttgt agcgtgttcc atcttgtagg ccttgttgcg agcatgttca
    10201 gatctactgt tccgctcttg attgagttat tggtgccttg ggttggtgca aacacaggct
    10261 ttaatatgtt atatctgttt tgtgtttgat gtagatctgt agggtagttc ttcttagaca
    10321 tggttcaatt atgtagcttg tgcgtttcga tttgatttca tatgttcaca gattagataa
    10381 tgatgaactc ttttaattaa ttgtcaatgg taaataggaa gtcttgtcgc tatatctgtc
    10441 ataatgatct catgttacta tctgccagta atttatgcta agaactatat tagaatatca
    10501 tgttacaatc tgtagtaata tcatgttaca atctgtagtt catctatata atctattgtg
    10561 gtaatttctt tttactatct gtgtgaagat tattgccact agttcattct acttatttct
    10621 gaagttcagg atacgtgtgc tgttactacc tatctgaata catgtgtgat gtgcctgtta
    10681 ctatcttttt gaatacatgt atgttctgtt ggaatatgtt tgctgtttga tccgttgttg
    10741 tgtccttaat cttgtgctag ttcttaccct atctgtttgg tgattatttc ttgcagatgc
    10801 agatctttgt gaagacattg accggcaaga ctatcaccct ggaggtggag tcctctgaca
    10861 ccatcgacaa tgtcaaggct aagatccaag ataaggaggg catccccccg gaccagcagc
    10921 gtctcatctt cgctggcaag cagctcgaag atggcaggac ccttgctgac tacaacatcc
    10981 agaaggagtc cacccttcac cttgtcctcc gcctccgtgg tggcggatcc atgaaaaagc
    11041 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
    11101 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
    11161 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
    11221 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta
    11281 gcgagagcct gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc
    11341 ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg
    11401 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
    11461 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
    11521 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
    11581 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
    11641 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
    11701 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
    11761 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg
    11821 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
    11881 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
    11941 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
    12001 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagttaa
    12061 tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
    12121 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
    12181 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
    12241 atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgc gtacggttaa
    12301 aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
    12361 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
    12421 gcacaaaatc accactcgat acaggcagcc catcagtcca ctagagacgc tcaccgggct
    12481 ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc
    12541 gtcgaagccg tgtgcgagac accgcagccg ccggcgttgt ggatacctcg cggaaaactt
    12601 ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc
    12661 ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc
    12721 gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct
    12781 ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc
    12841 gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt
    12901 tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg
    12961 ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa
    13021 ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg
    13081 aaccctcccg gcccgctctc gcgttggcag catcacccat aattgtggtt tcaaaatcgg
    13141 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
    13201 gtatttaaag ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
    13261 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
    13321 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
    13381 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
    13441 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
    13501 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc accttgaacg gcatgatggc
    13561 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
    13621 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
    13681 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
    13741 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacacccca
    13801 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
    13861 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
    13921 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
    13981 gtccggtcga tcagggagga tattggggaa gaacagtatg tcgagctatt ttttgactta
    14041 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
    14101 tagtacctag atgtggcgca acgatgctgg cgacaagcag gagcgcaccg acttcttccg
    14161 catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct
    14221 ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta
    14281 cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac caggcggatc
    14341 aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg
    14401 aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc
    14461 cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt
    14521 ccagtccgtc ggctcgatgg cccagcaagc tacggccaag atcgagcgcg acagcgtgca
    14581 actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga
    14641 acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac
    14701 caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaagc
    14761 cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat
    14821 tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt
    14881 caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt
    14941 caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact
    15001 ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt
    15061 cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa
    15121 ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt
    15181 tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa
    15241 aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca
    15301 ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt
    15361 cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat
    15421 gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga
    15481 agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg
    15541 caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca gcgctttact
    15601 gagatcctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    15661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    15721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    15781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    15841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    15901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    15961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    16021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    16081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    16141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    16201 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    16261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    16321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    16381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    16441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgg atctcctgtg
    16501 gttg
//
