LOCUS       sequence-267815-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (I744 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 I744 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     652..672
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     746..765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        746..764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3022..3042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3058..3078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4654..4701
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4702..4725
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4735..4791
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4792..5190
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5261..5278)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5267..5491
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5537..5965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5624..5643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5834..5855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5974..5994)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5979..6308
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6159..6294
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6221..6240
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6375..7169
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6429..6448)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7039..7058
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7343..7464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7380..7399)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7434..7453
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7513..7529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7513..7529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7526..7548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7537..7553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7561..7567,7568..7585,7586..7591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7606..7627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7744..7761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7915..8503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7995..8014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8674..9465,9466..9534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9297..9316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9535..9639)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9714..9733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
       61 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      121 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      181 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      241 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      301 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      361 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      421 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      481 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      541 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      601 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      661 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
      721 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
      781 agcgccacca tgaagcgaaa ttacattctg gggctggcca ttggcattac atcagtgggc
      841 tatggcatca ttgactacga gacaagggac gtgatcgacg ccggcgtgag actgttcaag
      901 gaggccaacg tggagaacaa tgagggccgg agatccaaga ggggagcaag gcgcctgaag
      961 cggagaaggc gccacagaat ccagagagtg aagaagctgc tgttcgatta caacctgctg
     1021 accgaccact ccgagctgtc tggcatcaat ccttatgagg ccagagtgaa gggcctgtcc
     1081 cagaagctgt ctgaggagga gtttagcgcc gccctgctgc acctggcaaa gaggagaggc
     1141 gtgcacaacg tgaatgaggt ggaggaggac accggcaacg agctgtccac aaaggagcag
     1201 atcagccgca attccaaggc cctggaggag aagtatgtgg ccgagctgca gctggagcgg
     1261 ctgaagaagg atggcgaggt gaggggctcc atcaatcgct tcaagacctc tgactacgtg
     1321 aaggaggcca agcagctgct gaaggtgcag aaggcctacc accagctgga tcagtccttt
     1381 atcgatacat atatcgacct gctggagaca aggcgcacat actatgaggg accaggagag
     1441 ggctctccct tcggctggaa ggacatcaag gagtggtacg agatgctgat gggccactgc
     1501 acctattttc cagaggagct gagaagcgtg aagtacgcct ataacgccga tctgtacaac
     1561 gccctgaatg acctgaacaa cctggtcatc accagggatg agaacgagaa gctggagtac
     1621 tatgagaagt tccagatcat cgagaacgtg ttcaagcaga agaagaagcc tacactgaag
     1681 cagatcgcca aggagatcct ggtgaacgag gaggacatca agggctaccg cgtgacctcc
     1741 acaggcaagc cagagttcac caatctgaag gtgtatcacg atatcaagga catcacagcc
     1801 cggaaggaga tcatcgagaa cgccgagctg ctggatcaga tcgccaagat cctgaccatc
     1861 tatcagagct ccgaggacat ccaggaggag ctgaccaacc tgaatagcga gctgacacag
     1921 gaggagatcg agcagatcag caatctgaag ggctacaccg gcacacacaa cctgagcctg
     1981 aaggccatca atctgatcct ggatgagctg tggcacacaa acgacaatca gatcgccatc
     2041 tttaaccggc tgaagctggt gccaaagaag gtggacctgt cccagcagaa ggagatccca
     2101 accacactgg tggacgattt catcctgtct cccgtggtga agcggagctt catccagagc
     2161 atcaaagtga tcaacgccat catcaagaag tacggcctgc ccaatgatat catcatcgag
     2221 ctggccaggg agaagaactc caaggacgcc cagaagatga tcaatgagat gcagaagagg
     2281 aaccgccaga ccaatgagcg gatcgaggag atcatcagaa ccacaggcaa ggagaacgcc
     2341 aagtacctga tcgagaagat caagctgcac gatatgcagg agggcaagtg tctgtattct
     2401 ctggaggcca tccctctgga ggacctgctg aacaatccat tcaactacga ggtggatcac
     2461 atcatccccc ggagcgtgag cttcgacaat tcttttaaca ataaggtgct ggtgaagcag
     2521 gaggagaaca gcaagaaggg caataggacc cctttccagt acctgtctag ctccgattct
     2581 aagatcagct acgagacatt caagaagcac atcctgaatc tggccaaggg caagggccgc
     2641 atcagcaaga ccaagaagga gtacctgctg gaggagcggg acatcaacag attctccgtg
     2701 cagaaggact tcatcaaccg gaatctggtg gacaccagat acgccacacg cggcctgatg
     2761 aatctgctgc ggtcttattt cagagtgaac aatctggatg tgaaggtgaa gagcatcaac
     2821 ggcggcttca cctcctttct gcggagaaag tggaagttta agaaggagcg caacaagggc
     2881 tataagcacc acgccgagga tgccctgatc atcgccaatg ccgacttcat ctttaaggag
     2941 tggaagaagc tggacaaggc caagaaagtg atggagaacc agatgttcga ggagaagcag
     3001 gccgagagca tgcccgagat cccgaagaag aaacgtaagg tgggtagcag cggtagcccg
     3061 aaaaagaagc gcaaagtggg cagcgacgcg ctggacgatt tcgatctgga catgctgggc
     3121 agcgatgcgc tggatgattt cggcggtggc agctctgagg tggagttttc ccacgagtac
     3181 tggatgagac atgccctgac cctggccaag agggcacgcg atgagaggga ggtgcctgtg
     3241 ggagccgtgc tggtgctgaa caatagagtg atcggcgagg gctggaacag agccatcggc
     3301 ctgcacgacc caacagccca tgccgaaatt atggccctga gacagggcgg cctggtcatg
     3361 cagaactaca gactgattga cgccaccctg tacggcacat tcgagccttg cgtgatgtgc
     3421 gccggcgcca tgatccactc taggatcggc cgcgtggtgt ttggcgtgag gaacgcaaaa
     3481 accggcgccg caggctccct gatggacgtg ctgcactacc ccggcatgaa tcaccgcgtc
     3541 gaaattaccg agggaatcct ggcagatgaa tgtgccgccc tgctgtgcta tttctttcgg
     3601 atgcctagac aggtgttcaa tgctcagaag aaggcccaga gctccaccga cggcggtagc
     3661 ggcggtagcg gtggtagcgg cggcagcggt ggtagcggtg gcagcggtgg tatcgagaca
     3721 gagcaggagt acaaggagat tttcatcaca cctcaccaga tcaagcacat caaggacttc
     3781 aaggactaca agtattctca cagggtggat aagaagccca accgcgagct gatcaatgac
     3841 accctgtata gcacacggaa ggacgataag ggcaataccc tgatcgtgaa caatctgaac
     3901 ggcctgtacg acaaggataa tgacaagctg aagaagctga tcaacaagtc tcccgagaag
     3961 ctgctgatgt accaccacga tcctcagaca tatcagaagc tgaagctgat catggagcag
     4021 tacggcgacg agaagaaccc actgtataag tactatgagg agacaggcaa ctacctgaca
     4081 aagtatagca agaaggataa tggccccgtg atcaagaaga tcaagtacta tggcaacaag
     4141 ctgaatgccc acctggacat caccgacgat taccctaact ctcgcaataa ggtggtgaag
     4201 ctgagcctga agccataccg gttcgacgtg tacctggaca acggcgtgta taagtttgtg
     4261 acagtgaaga atctggatgt gatcaagaag gagaactact atgaggtgaa cagcaagtgc
     4321 tacgaggagg ccaagaagct gaagaagatc agcaaccagg ccgagttcat cgcctctttt
     4381 tacaacaatg acctgatcaa gatcaatggc gagctgtata gagtgatcgg cgtgaacaat
     4441 gatctgctga acagaatcga agtgaatatg atcgacatca cctacaggga gtatctggag
     4501 aacatgaatg ataagaggcc ccctcgcatc atcaagacca tcgcctctaa gacacagagc
     4561 atcaagaagt acagcacaga catcctgggg aacctgtatg aagtcaagag caagaaacat
     4621 cctcagatta tcaagaaagg ctctggtggt tctaagcgac ctgccgccac aaagaaggct
     4681 ggacaggcta agaagaagaa agattacaaa gacgatgacg ataagggatc cggcgcaaca
     4741 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gatggccaag
     4801 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
     4861 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
     4921 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
     4981 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
     5041 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
     5101 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
     5161 ctgccctctg gttatgtgtg ggagggctaa gaattctgca gatatccagc acagtggcgg
     5221 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     5281 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5341 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5401 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5461 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5521 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5581 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5641 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5701 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5761 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5821 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5881 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5941 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     6001 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6061 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6121 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6181 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6241 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6301 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6361 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6421 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6481 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6541 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6601 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6661 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6721 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6781 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6841 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6901 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6961 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7021 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7081 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7141 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7201 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7261 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7321 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7441 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7501 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7561 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7621 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7681 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7741 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7801 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7861 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7921 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7981 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8041 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8101 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8161 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8221 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8281 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8341 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8521 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8581 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8641 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8701 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8761 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8821 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8881 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8941 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9001 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9061 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9661 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9721 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9781 gagtagt
//
