LOCUS       sequence-267817-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (H119 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 H119 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        132..511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        512..715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     666..686
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     760..779
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        760..778
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1161..1181
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4668..4715
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4716..4739
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4749..4805
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4806..5204
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5275..5292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5281..5505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5551..5979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5638..5657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5848..5869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5988..6008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5993..6322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6173..6308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6235..6254
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6389..7183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6443..6462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7053..7072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7357..7478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7394..7413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7448..7467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7527..7543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7527..7543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7540..7562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7551..7567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7575..7581,7582..7599,7600..7605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7620..7641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7758..7775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7929..8517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8009..8028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8688..9479,9480..9548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9311..9330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9549..9653)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9728..9747)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
       61 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      121 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      361 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      421 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      481 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      541 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      601 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      661 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      721 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      781 gacccaagct ggctagcgcc accatgaagc gaaattacat tctggggctg gccattggca
      841 ttacatcagt gggctatggc atcattgact acgagacaag ggacgtgatc gacgccggcg
      901 tgagactgtt caaggaggcc aacgtggaga acaatgaggg ccggagatcc aagaggggag
      961 caaggcgcct gaagcggaga aggcgccaca gaatccagag agtgaagaag ctgctgttcg
     1021 attacaacct gctgaccgac cactccgagc tgtctggcat caatccttat gaggccagag
     1081 tgaagggcct gtcccagaag ctgtctgagg aggagtttag cgccgccctg ctgcacctgg
     1141 caaagaggag aggcgtgcac ccgaagaaga aacgtaaggt gggtagcagc ggtagcccga
     1201 aaaagaagcg caaagtgggc agcgacgcgc tggacgattt cgatctggac atgctgggca
     1261 gcgatgcgct ggatgatttc ggcggtggca gctctgaggt ggagttttcc cacgagtact
     1321 ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag gtgcctgtgg
     1381 gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga gccatcggcc
     1441 tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc ctggtcatgc
     1501 agaactacag actgattgac gccaccctgt acggcacatt cgagccttgc gtgatgtgcg
     1561 ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa
     1621 ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat caccgcgtcg
     1681 aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat ttctttcgga
     1741 tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac ggcggtagcg
     1801 gcggtagcgg tggtagcggc ggcagcggtg gtagcggtgg cagcggtggt cacaacgtga
     1861 atgaggtgga ggaggacacc ggcaacgagc tgtccacaaa ggagcagatc agccgcaatt
     1921 ccaaggccct ggaggagaag tatgtggccg agctgcagct ggagcggctg aagaaggatg
     1981 gcgaggtgag gggctccatc aatcgcttca agacctctga ctacgtgaag gaggccaagc
     2041 agctgctgaa ggtgcagaag gcctaccacc agctggatca gtcctttatc gatacatata
     2101 tcgacctgct ggagacaagg cgcacatact atgagggacc aggagagggc tctcccttcg
     2161 gctggaagga catcaaggag tggtacgaga tgctgatggg ccactgcacc tattttccag
     2221 aggagctgag aagcgtgaag tacgcctata acgccgatct gtacaacgcc ctgaatgacc
     2281 tgaacaacct ggtcatcacc agggatgaga acgagaagct ggagtactat gagaagttcc
     2341 agatcatcga gaacgtgttc aagcagaaga agaagcctac actgaagcag atcgccaagg
     2401 agatcctggt gaacgaggag gacatcaagg gctaccgcgt gacctccaca ggcaagccag
     2461 agttcaccaa tctgaaggtg tatcacgata tcaaggacat cacagcccgg aaggagatca
     2521 tcgagaacgc cgagctgctg gatcagatcg ccaagatcct gaccatctat cagagctccg
     2581 aggacatcca ggaggagctg accaacctga atagcgagct gacacaggag gagatcgagc
     2641 agatcagcaa tctgaagggc tacaccggca cacacaacct gagcctgaag gccatcaatc
     2701 tgatcctgga tgagctgtgg cacacaaacg acaatcagat cgccatcttt aaccggctga
     2761 agctggtgcc aaagaaggtg gacctgtccc agcagaagga gatcccaacc acactggtgg
     2821 acgatttcat cctgtctccc gtggtgaagc ggagcttcat ccagagcatc aaagtgatca
     2881 acgccatcat caagaagtac ggcctgccca atgatatcat catcgagctg gccagggaga
     2941 agaactccaa ggacgcccag aagatgatca atgagatgca gaagaggaac cgccagacca
     3001 atgagcggat cgaggagatc atcagaacca caggcaagga gaacgccaag tacctgatcg
     3061 agaagatcaa gctgcacgat atgcaggagg gcaagtgtct gtattctctg gaggccatcc
     3121 ctctggagga cctgctgaac aatccattca actacgaggt ggatcacatc atcccccgga
     3181 gcgtgagctt cgacaattct tttaacaata aggtgctggt gaagcaggag gagaacagca
     3241 agaagggcaa taggacccct ttccagtacc tgtctagctc cgattctaag atcagctacg
     3301 agacattcaa gaagcacatc ctgaatctgg ccaagggcaa gggccgcatc agcaagacca
     3361 agaaggagta cctgctggag gagcgggaca tcaacagatt ctccgtgcag aaggacttca
     3421 tcaaccggaa tctggtggac accagatacg ccacacgcgg cctgatgaat ctgctgcggt
     3481 cttatttcag agtgaacaat ctggatgtga aggtgaagag catcaacggc ggcttcacct
     3541 cctttctgcg gagaaagtgg aagtttaaga aggagcgcaa caagggctat aagcaccacg
     3601 ccgaggatgc cctgatcatc gccaatgccg acttcatctt taaggagtgg aagaagctgg
     3661 acaaggccaa gaaagtgatg gagaaccaga tgttcgagga gaagcaggcc gagagcatgc
     3721 ccgagatcga gacagagcag gagtacaagg agattttcat cacacctcac cagatcaagc
     3781 acatcaagga cttcaaggac tacaagtatt ctcacagggt ggataagaag cccaaccgcg
     3841 agctgatcaa tgacaccctg tatagcacac ggaaggacga taagggcaat accctgatcg
     3901 tgaacaatct gaacggcctg tacgacaagg ataatgacaa gctgaagaag ctgatcaaca
     3961 agtctcccga gaagctgctg atgtaccacc acgatcctca gacatatcag aagctgaagc
     4021 tgatcatgga gcagtacggc gacgagaaga acccactgta taagtactat gaggagacag
     4081 gcaactacct gacaaagtat agcaagaagg ataatggccc cgtgatcaag aagatcaagt
     4141 actatggcaa caagctgaat gcccacctgg acatcaccga cgattaccct aactctcgca
     4201 ataaggtggt gaagctgagc ctgaagccat accggttcga cgtgtacctg gacaacggcg
     4261 tgtataagtt tgtgacagtg aagaatctgg atgtgatcaa gaaggagaac tactatgagg
     4321 tgaacagcaa gtgctacgag gaggccaaga agctgaagaa gatcagcaac caggccgagt
     4381 tcatcgcctc tttttacaac aatgacctga tcaagatcaa tggcgagctg tatagagtga
     4441 tcggcgtgaa caatgatctg ctgaacagaa tcgaagtgaa tatgatcgac atcacctaca
     4501 gggagtatct ggagaacatg aatgataaga ggccccctcg catcatcaag accatcgcct
     4561 ctaagacaca gagcatcaag aagtacagca cagacatcct ggggaacctg tatgaagtca
     4621 agagcaagaa acatcctcag attatcaaga aaggctctgg tggttctaag cgacctgccg
     4681 ccacaaagaa ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg
     4741 gatccggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg
     4801 gaccgatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     4861 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     4921 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     4981 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     5041 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     5101 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     5161 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagaattc tgcagatatc
     5221 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     5281 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5341 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5401 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5461 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5521 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5581 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5641 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5701 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5761 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5821 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5881 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5941 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6001 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6061 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6121 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6241 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6301 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6361 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6421 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6481 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6541 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6601 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6661 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6721 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6781 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6841 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6901 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6961 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7021 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7081 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7141 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7201 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7261 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7321 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7381 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7441 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7501 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7561 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7621 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7681 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7741 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7801 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7861 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7921 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7981 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8041 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8101 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8161 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8221 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8281 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8341 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8401 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8461 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8521 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8581 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8641 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8701 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8761 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8821 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8881 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8941 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9001 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9061 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9121 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9181 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9241 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9301 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9361 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9421 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9481 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9541 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9601 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9661 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     9721 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     9781 tgttgga
//
