LOCUS       sequence-26782-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Largman Plasmids
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXA9-pRSET
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..99
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(103..111,112..126)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             439..456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgcggg gttctcatca tcatcatcat
       61 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      121 gataaggatc gatggatccc ccgggctgca ggaattcggc acgaggcggg ccaggcgctg
      181 ggcacggtga tggccaccac tggggccctg ggcaactact acgtggactc gttcctgctg
      241 ggcgccgacg ccgcggatga gctgagcgtt ggccgctatg cgccggggac cctgggccag
      301 cctccccggc aggcggcgac gctggccgag caccccgact tcagcccgtg cagcttccag
      361 tccaaggcga cggtgtttgg cgcctcgtgg aacccagtgc acgcggcggg cgccaacgct
      421 gtacccgctg cggtgtacca ccaccatcac caccacccct acgtgcaccc ccaggcgccc
      481 gtggcggcgg cggcgccgga cggcaggtac atgcgctcct ggctggagcc cacgcccggt
      541 gcgctctcct tcgcgggctt gccctccagc cggccttatg gcattaaacc tgaaccgctg
      601 tcggccagaa ggggtgactg tcccacgctt gacactcaca ctttgtccct gactgactat
      661 gcttgtggtt ctcctccagt tgatagagaa aaacaaccca gcgaaagcgc cttctctgaa
      721 aacaatgctg agaatgagag cggcggagac agccccccat cgatcccaat aacccagcag
      781 ccaactggct tcatgcgcgc tccactcgga aaaag
//
