LOCUS       sequence-267820-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (M741 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 M741 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        148..527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     682..702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     776..795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        776..794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3043..3063
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3079..3099
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4684..4731
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4732..4755
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4765..4821
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4822..5220
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5291..5308)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5297..5521
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5567..5995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5654..5673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5864..5885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6004..6024)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6009..6338
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6189..6324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6251..6270
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6405..7199
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6459..6478)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7069..7088
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7373..7494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7410..7429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7464..7483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7543..7559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7543..7559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7556..7578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7567..7583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7591..7597,7598..7615,7616..7621))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7636..7657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7774..7791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7945..8533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8025..8044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8704..9495,9496..9564))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9327..9346
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9565..9669)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9744..9763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
       61 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      121 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      721 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
      781 cgactcacta tagggagacc caagctggct agcgccacca tgaagcgaaa ttacattctg
      841 gggctggcca ttggcattac atcagtgggc tatggcatca ttgactacga gacaagggac
      901 gtgatcgacg ccggcgtgag actgttcaag gaggccaacg tggagaacaa tgagggccgg
      961 agatccaaga ggggagcaag gcgcctgaag cggagaaggc gccacagaat ccagagagtg
     1021 aagaagctgc tgttcgatta caacctgctg accgaccact ccgagctgtc tggcatcaat
     1081 ccttatgagg ccagagtgaa gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc
     1141 gccctgctgc acctggcaaa gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac
     1201 accggcaacg agctgtccac aaaggagcag atcagccgca attccaaggc cctggaggag
     1261 aagtatgtgg ccgagctgca gctggagcgg ctgaagaagg atggcgaggt gaggggctcc
     1321 atcaatcgct tcaagacctc tgactacgtg aaggaggcca agcagctgct gaaggtgcag
     1381 aaggcctacc accagctgga tcagtccttt atcgatacat atatcgacct gctggagaca
     1441 aggcgcacat actatgaggg accaggagag ggctctccct tcggctggaa ggacatcaag
     1501 gagtggtacg agatgctgat gggccactgc acctattttc cagaggagct gagaagcgtg
     1561 aagtacgcct ataacgccga tctgtacaac gccctgaatg acctgaacaa cctggtcatc
     1621 accagggatg agaacgagaa gctggagtac tatgagaagt tccagatcat cgagaacgtg
     1681 ttcaagcaga agaagaagcc tacactgaag cagatcgcca aggagatcct ggtgaacgag
     1741 gaggacatca agggctaccg cgtgacctcc acaggcaagc cagagttcac caatctgaag
     1801 gtgtatcacg atatcaagga catcacagcc cggaaggaga tcatcgagaa cgccgagctg
     1861 ctggatcaga tcgccaagat cctgaccatc tatcagagct ccgaggacat ccaggaggag
     1921 ctgaccaacc tgaatagcga gctgacacag gaggagatcg agcagatcag caatctgaag
     1981 ggctacaccg gcacacacaa cctgagcctg aaggccatca atctgatcct ggatgagctg
     2041 tggcacacaa acgacaatca gatcgccatc tttaaccggc tgaagctggt gccaaagaag
     2101 gtggacctgt cccagcagaa ggagatccca accacactgg tggacgattt catcctgtct
     2161 cccgtggtga agcggagctt catccagagc atcaaagtga tcaacgccat catcaagaag
     2221 tacggcctgc ccaatgatat catcatcgag ctggccaggg agaagaactc caaggacgcc
     2281 cagaagatga tcaatgagat gcagaagagg aaccgccaga ccaatgagcg gatcgaggag
     2341 atcatcagaa ccacaggcaa ggagaacgcc aagtacctga tcgagaagat caagctgcac
     2401 gatatgcagg agggcaagtg tctgtattct ctggaggcca tccctctgga ggacctgctg
     2461 aacaatccat tcaactacga ggtggatcac atcatccccc ggagcgtgag cttcgacaat
     2521 tcttttaaca ataaggtgct ggtgaagcag gaggagaaca gcaagaaggg caataggacc
     2581 cctttccagt acctgtctag ctccgattct aagatcagct acgagacatt caagaagcac
     2641 atcctgaatc tggccaaggg caagggccgc atcagcaaga ccaagaagga gtacctgctg
     2701 gaggagcggg acatcaacag attctccgtg cagaaggact tcatcaaccg gaatctggtg
     2761 gacaccagat acgccacacg cggcctgatg aatctgctgc ggtcttattt cagagtgaac
     2821 aatctggatg tgaaggtgaa gagcatcaac ggcggcttca cctcctttct gcggagaaag
     2881 tggaagttta agaaggagcg caacaagggc tataagcacc acgccgagga tgccctgatc
     2941 atcgccaatg ccgacttcat ctttaaggag tggaagaagc tggacaaggc caagaaagtg
     3001 atggagaacc agatgttcga ggagaagcag gccgagagca tgccgaagaa gaaacgtaag
     3061 gtgggtagca gcggtagccc gaaaaagaag cgcaaagtgg gcagcgacgc gctggacgat
     3121 ttcgatctgg acatgctggg cagcgatgcg ctggatgatt tcggcggtgg cagctctgag
     3181 gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc
     3241 gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag
     3301 ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg
     3361 agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacggcaca
     3421 ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg
     3481 tttggcgtga ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac
     3541 cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc
     3601 ctgctgtgct atttctttcg gatgcctaga caggtgttca atgctcagaa gaaggcccag
     3661 agctccaccg acggcggtag cggcggtagc ggtggtagcg gcggcagcgg tggtagcggt
     3721 ggcagcggtg gtatgcccga gatcgagaca gagcaggagt acaaggagat tttcatcaca
     3781 cctcaccaga tcaagcacat caaggacttc aaggactaca agtattctca cagggtggat
     3841 aagaagccca accgcgagct gatcaatgac accctgtata gcacacggaa ggacgataag
     3901 ggcaataccc tgatcgtgaa caatctgaac ggcctgtacg acaaggataa tgacaagctg
     3961 aagaagctga tcaacaagtc tcccgagaag ctgctgatgt accaccacga tcctcagaca
     4021 tatcagaagc tgaagctgat catggagcag tacggcgacg agaagaaccc actgtataag
     4081 tactatgagg agacaggcaa ctacctgaca aagtatagca agaaggataa tggccccgtg
     4141 atcaagaaga tcaagtacta tggcaacaag ctgaatgccc acctggacat caccgacgat
     4201 taccctaact ctcgcaataa ggtggtgaag ctgagcctga agccataccg gttcgacgtg
     4261 tacctggaca acggcgtgta taagtttgtg acagtgaaga atctggatgt gatcaagaag
     4321 gagaactact atgaggtgaa cagcaagtgc tacgaggagg ccaagaagct gaagaagatc
     4381 agcaaccagg ccgagttcat cgcctctttt tacaacaatg acctgatcaa gatcaatggc
     4441 gagctgtata gagtgatcgg cgtgaacaat gatctgctga acagaatcga agtgaatatg
     4501 atcgacatca cctacaggga gtatctggag aacatgaatg ataagaggcc ccctcgcatc
     4561 atcaagacca tcgcctctaa gacacagagc atcaagaagt acagcacaga catcctgggg
     4621 aacctgtatg aagtcaagag caagaaacat cctcagatta tcaagaaagg ctctggtggt
     4681 tctaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa
     4741 gacgatgacg ataagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     4801 gtcgaagaga atcctggacc gatggccaag cctttgtctc aagaagaatc caccctcatt
     4861 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     4921 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     4981 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     5041 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     5101 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     5161 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     5221 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
     5281 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5341 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5401 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5461 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5521 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5581 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5641 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5701 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5761 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5821 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5881 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5941 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6001 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6061 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6181 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6241 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6301 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6361 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     6421 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     6481 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     6541 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     6601 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6661 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6721 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6781 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6841 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6901 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6961 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7021 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7081 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7141 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7201 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7261 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7321 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7381 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7441 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7501 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     7561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7621 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7681 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7741 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8581 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8641 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8701 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8761 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8821 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8881 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8941 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9001 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9061 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9121 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9181 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9241 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9301 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9361 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9421 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9481 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9541 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9601 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9661 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9721 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9781 ctgctcc
//
