LOCUS       sequence-267821-        9654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF5B 376-963.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF5B
FEATURES             Location/Qualifiers
     source          1..9654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..275
                     /label=chicken beta-actin promoter
     primer_bind     1218..1240
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1308..1310,1311..1313,1314..2021)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1353..1374)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1614..1633)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1961..1982
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5073..5207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5110..5129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5164..5183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5238..5254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5238..5254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5251..5273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5262..5278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5286..5292,5293..5310,5311..5316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5331..5352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5469..5486)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5640..6228)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5720..5739)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6399..7190,7191..7259))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7022..7041
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7260..7364)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7432..7450
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7488..7510)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7610..7629
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7823..7845
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7837..7854
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7838..7854
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggc
      121 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
      181 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
      241 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc
      301 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
      361 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag cgcttggttt
      421 aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc cgggagggcc
      481 ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
      541 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
      601 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
      661 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
      721 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
      781 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
      841 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
      901 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
      961 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1021 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1081 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     1141 ccgtcccctt ctccatctcc agcctcgggg ctgtccgcag ggggacggct gccttcgggg
     1201 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt atatcttccc
     1261 ttctctgttc ctccgcagcc cccaagcttg gcgcgcctag gcctaccatg gtgagcaagg
     1321 gcggggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta catgacgcgt gaattcggga
     2041 agctggccgc tatacgtacg atggcggacc cggcggagtg caacatcaaa gtgatgtgtc
     2101 gcttcagacc tctcaacgaa tctgaagtga accgcggcga taagtacgtc gccaaattcc
     2161 agggagaaga cacggtgatg atcgcgtcca agccttatgc ctttgatcgt gtgttccagt
     2221 caagcacatc tcaagagcaa gtatacaacg actgtgcaaa aaagattgtt aaagatgttc
     2281 ttgagggcta taatggaaca atatttgcat atggacaaac atcatctggg aagacccaca
     2341 cgatggaggg taaacttcat gatccagaag gcatgggaat tattccaaga atagtgcaag
     2401 atatttttaa ttatatttac tccatggatg aaaatttgga atttcatatt aaggtttcat
     2461 attttgaaat atatttggat aagataaggg acttgttaga tgtttcaaag actaaccttt
     2521 cagtccatga agacaaaaac cgtgttccct atgtaaaggg gtgcacagaa cgtttcgtgt
     2581 gtagtccaga tgaagtcatg gataccatag atgaagggaa atccaacaga catgtcgcag
     2641 ttacaaatat gaatgaacat agctctagga gccacagcat atttcttatt aatgtaaaac
     2701 aagagaatac acaaacggaa cagaaactca gtggaaagct ttatctggtt gatttagctg
     2761 gcagtgagaa ggttagtaag actggggctg aaggtgctgt gctggatgaa gctaagaaca
     2821 tcaacaagtc actttctgca cttggaaatg tcatttctgc tttggcagag ggcagtacct
     2881 atgttcctta tcgagatagt aaaatgacca gaattcttca agattcatta ggtggcaact
     2941 gtaggaccac tattgtcata tgctgctctc catcatcata caatgagtct gagacaaagt
     3001 caacactcct ctttggtcaa agggccaaaa caattaagaa cacagtctgt gtcaatgtag
     3061 agttaactgc agagcagtgg aaaaagaagt atgaaaaaga aaaggaaaaa aataagactc
     3121 tacggaacac tattcagtgg ctggaaaacg agctaaaccg ttggcgtaac ggggagacag
     3181 tgcctattga tgagcagttt gacaaagaga aagctaattt ggaagccttc acagcggata
     3241 aagatactgc tattaccagt gataaaccag ctgctgcagt cggaatggct ggtagtttta
     3301 ccgatgctga aagaagaaag tgtgaagaag aacttgctaa attgtataaa cagcttgatg
     3361 acaaggatga agagattaac caacaaagcc aattggtaga gaaattgaag acacaaatgc
     3421 tggatcagga agagcttctg gcatcaacca gaagggatca agataatatg caagctgaac
     3481 tgaaccgcct ccaagcagaa aatgatgctt ctaaagaaga agtcaaagaa gttttacagg
     3541 ccttagagga actggctgtt aattatgatc agaagtctca ggaagttgaa gacaaaacaa
     3601 aggaatatga attgcttagt gatgaattga atcaaaaatc tgcaacttta gcaagtattg
     3661 atgctgagct tcagaagctg aaggaaatga ccaaccacca gaagaaacga gcagctgaaa
     3721 tgatggcatc attattaaaa gaccttgcag aaataggaat tgctgtgggg aataacgatg
     3781 tgaagcaacc agaaggaact ggtatgatag atgaagagtt tactgttgca agactctaca
     3841 ttagcaaaat gaaatcagaa gtaaagacca tggtgaaacg ctgcaaacag ctagaaagca
     3901 cgcagactga gagcaacaaa aaaatggaag aaaatgagaa agagttagca gcatgccagc
     3961 ttcggatctc ccaacatgaa gccaaaatca agtcactgac tgagtacctt cagaatgtag
     4021 aacaaaagaa gaggcagctg gaggaatctg ttgattccct tggtgaggag ctagtccaac
     4081 tccgagcaca agagaaagtc catgaaatgg aaaaagagca cttgaacaag gttcagactg
     4141 caaatgaagt caagcaagct gttgagcagc agatccagag tcacagagaa acccaccaaa
     4201 aacaaatcag tagcttgcga gatgaagttg aggcaaagga aaagctaatc actgacctcc
     4261 aagaccaaaa ccagaagatg gtgttggagc aggaacggct aagggtggag catgagaggc
     4321 tgaaggctac agaccaagag aagagcagga agctgcatga gctcacggtt atgcaagaca
     4381 gacgagaaca agcaagacaa gacttgaagg gtttggagga gaccgtggca aaagaacttc
     4441 agactttaca caacctgcgt aagctctttg ttcaggactt ggctaccagg gtgaaaaaga
     4501 gcgccgaggt cgactctgac gacactggcg gcagtgctgc acagaagcag aaaatctcct
     4561 tccttgaaaa caaccttgaa cagctcacca aagtgcacaa gcagttggta cgtgataatg
     4621 cagatcttcg ctgtgagcat cctaagttag agaaacggct tagagctact gcagaaagag
     4681 tgaaagcttt ggagtcagcc ctgaaagaag ccaaagaaaa tgcatctcga gaccgtaaac
     4741 gctatcagca agaagtagac cgaataaagg aagcagtcag gtcaaagaat atggccagaa
     4801 ggggacattc tgcccagatt gcaaagccta tccgccctgg acagcatcca gcggcctcgc
     4861 caactcaccc aggcacagtt cgtggaggag gctcatttgt tcagaacaac cagccagtgg
     4921 ggcttcgtgg tggtggaggc aagcagtcgt aatctagata agtaatgatc ataatcagcc
     4981 atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5041 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5101 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5161 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag
     5221 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5281 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5341 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5401 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5461 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5521 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5581 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5641 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5701 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5761 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5821 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5881 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5941 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6001 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6061 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6121 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6181 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6241 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6301 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6361 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6421 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6481 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6541 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6601 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6661 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6721 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6781 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6841 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6901 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6961 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7021 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7081 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7141 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7201 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7261 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7321 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7381 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7441 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7501 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7561 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     7621 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7681 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7741 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7801 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     7861 ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt cagcacccgg
     7921 ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg tgaggagccc
     7981 cctaccagca gcacacagtg gggttggcac tgccacgctc cggatgccgc gctctgatcc
     8041 aaccccataa tcaagggaac ccgaattgcc ccatcattgc ccccaccacc cccatcctgc
     8101 cgggccctca caccccacgc tgccttctgg tgacattccc cagcccaaac ccacggcttc
     8161 atggctaccg cggggcattt cccattgccg ccccattatc agctctgcac acctcccgct
     8221 gtacccatgc ctcgtggctg cccttctttg acgtataatc ttctaattaa tacccggcct
     8281 tgtcaaagtg gagcacaaac gttaattaat tccccagcag gcaggtaatt aacagtgtga
     8341 ctcccttttt gctgcgagtg gggctgatac agagagatgt ggcactatgg agcccacggg
     8401 gtcctggcac tgggtgccca cggaggtccc catgtgctgc agtgtcaccg cctccgagga
     8461 gacagtattg tccctgcggt gtccctgcag ctcagctctg tccacagggc cacctccagt
     8521 ttggagggga cacaatgcag ccccgatgca acccatcctc gcagcatccc agggacaaag
     8581 accccactgc aagaccgcac acagggctgg gtcccgctcc cctaatatct acagtgcttc
     8641 ttgcatggcc ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct
     8701 gcaaagcccc tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc
     8761 acgcgctgca aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct
     8821 gccttgacct ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc
     8881 ctccctccgg ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct
     8941 ctgaggtgca cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc
     9001 tacgtgctga cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg
     9061 gtgtagagag tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca
     9121 ggctgcaccc cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc
     9181 atgcatgcgt cttgatggtg cagcgctgtg catgcatcct ccttggtgtg tagcagtcta
     9241 gtgcatgcat acccctcggt gttattgctg ctctgtgcac gcacgctcat tgtatcactt
     9301 catcccagtg catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt
     9361 atcactgcag ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc
     9421 acatcagtgt cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc
     9481 tcctgctggc gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg
     9541 ggggagggct gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa
     9601 aaggggggag gggggatgga ggggcgcgtc acacccccgc cccacaccct cacc
//
