LOCUS       sequence-267822-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (N120 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 N120 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        164..543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        544..747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     698..718
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     792..811
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        792..810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1196..1216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1232..1252
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4700..4747
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4748..4771
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4781..4837
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4838..5236
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5307..5324)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5313..5537
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5583..6011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5670..5689)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5880..5901
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6020..6040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6025..6354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6205..6340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6267..6286
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6421..7215
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6475..6494)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7085..7104
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7389..7510
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7426..7445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7480..7499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7559..7575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7559..7575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7572..7594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7583..7599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7607..7613,7614..7631,7632..7637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7652..7673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7790..7807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7961..8549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8041..8060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8720..9511,9512..9580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9343..9362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9581..9685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9760..9779)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
       61 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
      121 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      361 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      721 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
      781 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg ccaccatgaa
      841 gcgaaattac attctggggc tggccattgg cattacatca gtgggctatg gcatcattga
      901 ctacgagaca agggacgtga tcgacgccgg cgtgagactg ttcaaggagg ccaacgtgga
      961 gaacaatgag ggccggagat ccaagagggg agcaaggcgc ctgaagcgga gaaggcgcca
     1021 cagaatccag agagtgaaga agctgctgtt cgattacaac ctgctgaccg accactccga
     1081 gctgtctggc atcaatcctt atgaggccag agtgaagggc ctgtcccaga agctgtctga
     1141 ggaggagttt agcgccgccc tgctgcacct ggcaaagagg agaggcgtgc acaacccgaa
     1201 gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag aagcgcaaag tgggcagcga
     1261 cgcgctggac gatttcgatc tggacatgct gggcagcgat gcgctggatg atttcggcgg
     1321 tggcagctct gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc
     1381 caagagggca cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag
     1441 agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga
     1501 aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac
     1561 cctgtacggc acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat
     1621 cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga
     1681 cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
     1741 tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca
     1801 gaagaaggcc cagagctcca ccgacggcgg tagcggcggt agcggtggta gcggcggcag
     1861 cggtggtagc ggtggcagcg gtggtaacgt gaatgaggtg gaggaggaca ccggcaacga
     1921 gctgtccaca aaggagcaga tcagccgcaa ttccaaggcc ctggaggaga agtatgtggc
     1981 cgagctgcag ctggagcggc tgaagaagga tggcgaggtg aggggctcca tcaatcgctt
     2041 caagacctct gactacgtga aggaggccaa gcagctgctg aaggtgcaga aggcctacca
     2101 ccagctggat cagtccttta tcgatacata tatcgacctg ctggagacaa ggcgcacata
     2161 ctatgaggga ccaggagagg gctctccctt cggctggaag gacatcaagg agtggtacga
     2221 gatgctgatg ggccactgca cctattttcc agaggagctg agaagcgtga agtacgccta
     2281 taacgccgat ctgtacaacg ccctgaatga cctgaacaac ctggtcatca ccagggatga
     2341 gaacgagaag ctggagtact atgagaagtt ccagatcatc gagaacgtgt tcaagcagaa
     2401 gaagaagcct acactgaagc agatcgccaa ggagatcctg gtgaacgagg aggacatcaa
     2461 gggctaccgc gtgacctcca caggcaagcc agagttcacc aatctgaagg tgtatcacga
     2521 tatcaaggac atcacagccc ggaaggagat catcgagaac gccgagctgc tggatcagat
     2581 cgccaagatc ctgaccatct atcagagctc cgaggacatc caggaggagc tgaccaacct
     2641 gaatagcgag ctgacacagg aggagatcga gcagatcagc aatctgaagg gctacaccgg
     2701 cacacacaac ctgagcctga aggccatcaa tctgatcctg gatgagctgt ggcacacaaa
     2761 cgacaatcag atcgccatct ttaaccggct gaagctggtg ccaaagaagg tggacctgtc
     2821 ccagcagaag gagatcccaa ccacactggt ggacgatttc atcctgtctc ccgtggtgaa
     2881 gcggagcttc atccagagca tcaaagtgat caacgccatc atcaagaagt acggcctgcc
     2941 caatgatatc atcatcgagc tggccaggga gaagaactcc aaggacgccc agaagatgat
     3001 caatgagatg cagaagagga accgccagac caatgagcgg atcgaggaga tcatcagaac
     3061 cacaggcaag gagaacgcca agtacctgat cgagaagatc aagctgcacg atatgcagga
     3121 gggcaagtgt ctgtattctc tggaggccat ccctctggag gacctgctga acaatccatt
     3181 caactacgag gtggatcaca tcatcccccg gagcgtgagc ttcgacaatt cttttaacaa
     3241 taaggtgctg gtgaagcagg aggagaacag caagaagggc aataggaccc ctttccagta
     3301 cctgtctagc tccgattcta agatcagcta cgagacattc aagaagcaca tcctgaatct
     3361 ggccaagggc aagggccgca tcagcaagac caagaaggag tacctgctgg aggagcggga
     3421 catcaacaga ttctccgtgc agaaggactt catcaaccgg aatctggtgg acaccagata
     3481 cgccacacgc ggcctgatga atctgctgcg gtcttatttc agagtgaaca atctggatgt
     3541 gaaggtgaag agcatcaacg gcggcttcac ctcctttctg cggagaaagt ggaagtttaa
     3601 gaaggagcgc aacaagggct ataagcacca cgccgaggat gccctgatca tcgccaatgc
     3661 cgacttcatc tttaaggagt ggaagaagct ggacaaggcc aagaaagtga tggagaacca
     3721 gatgttcgag gagaagcagg ccgagagcat gcccgagatc gagacagagc aggagtacaa
     3781 ggagattttc atcacacctc accagatcaa gcacatcaag gacttcaagg actacaagta
     3841 ttctcacagg gtggataaga agcccaaccg cgagctgatc aatgacaccc tgtatagcac
     3901 acggaaggac gataagggca ataccctgat cgtgaacaat ctgaacggcc tgtacgacaa
     3961 ggataatgac aagctgaaga agctgatcaa caagtctccc gagaagctgc tgatgtacca
     4021 ccacgatcct cagacatatc agaagctgaa gctgatcatg gagcagtacg gcgacgagaa
     4081 gaacccactg tataagtact atgaggagac aggcaactac ctgacaaagt atagcaagaa
     4141 ggataatggc cccgtgatca agaagatcaa gtactatggc aacaagctga atgcccacct
     4201 ggacatcacc gacgattacc ctaactctcg caataaggtg gtgaagctga gcctgaagcc
     4261 ataccggttc gacgtgtacc tggacaacgg cgtgtataag tttgtgacag tgaagaatct
     4321 ggatgtgatc aagaaggaga actactatga ggtgaacagc aagtgctacg aggaggccaa
     4381 gaagctgaag aagatcagca accaggccga gttcatcgcc tctttttaca acaatgacct
     4441 gatcaagatc aatggcgagc tgtatagagt gatcggcgtg aacaatgatc tgctgaacag
     4501 aatcgaagtg aatatgatcg acatcaccta cagggagtat ctggagaaca tgaatgataa
     4561 gaggccccct cgcatcatca agaccatcgc ctctaagaca cagagcatca agaagtacag
     4621 cacagacatc ctggggaacc tgtatgaagt caagagcaag aaacatcctc agattatcaa
     4681 gaaaggctct ggtggttcta agcgacctgc cgccacaaag aaggctggac aggctaagaa
     4741 gaagaaagat tacaaagacg atgacgataa gggatccggc gcaacaaact tctctctgct
     4801 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gccaagcctt tgtctcaaga
     4861 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     4921 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     4981 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     5041 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     5101 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     5161 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     5221 tgtgtgggag ggctaagaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     5281 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5341 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5401 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5461 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5521 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5581 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5641 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5701 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5761 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5821 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5881 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5941 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6001 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6061 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6121 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6181 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     6301 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6361 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6421 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6481 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6541 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6601 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6661 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6721 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6781 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6841 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6901 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6961 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     7021 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     7081 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     7141 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     7201 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     7261 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     7321 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     7381 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7501 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     7561 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7621 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7681 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7741 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7801 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7861 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7921 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7981 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     8041 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     8101 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8161 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8221 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8281 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8341 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8401 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8461 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8521 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8581 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8641 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8701 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8761 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8821 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8881 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8941 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9001 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9061 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9121 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9181 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9241 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9301 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9361 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9421 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9481 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9541 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9601 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9661 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9721 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9781 atagtta
//
