LOCUS       sequence-267823-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (P742 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 P742 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        150..529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        530..733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     684..704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     778..797
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        778..796
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3048..3068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3084..3104
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4686..4733
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4734..4757
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4767..4823
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4824..5222
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5293..5310)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5299..5523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5569..5997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5656..5675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5866..5887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6006..6026)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6011..6340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6191..6326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6253..6272
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6407..7201
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6461..6480)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7071..7090
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7375..7496
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7412..7431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7466..7485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7545..7561)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7545..7561)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7558..7580)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7569..7585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7593..7599,7600..7617,7618..7623))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7638..7659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7776..7793)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7947..8535)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8027..8046)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8706..9497,9498..9566))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9329..9348
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9567..9671)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9746..9765)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
       61 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      121 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      721 tataagcaga gctctctggc taactagaga acccactgct tactggctta tcgaaattaa
      781 tacgactcac tatagggaga cccaagctgg ctagcgccac catgaagcga aattacattc
      841 tggggctggc cattggcatt acatcagtgg gctatggcat cattgactac gagacaaggg
      901 acgtgatcga cgccggcgtg agactgttca aggaggccaa cgtggagaac aatgagggcc
      961 ggagatccaa gaggggagca aggcgcctga agcggagaag gcgccacaga atccagagag
     1021 tgaagaagct gctgttcgat tacaacctgc tgaccgacca ctccgagctg tctggcatca
     1081 atccttatga ggccagagtg aagggcctgt cccagaagct gtctgaggag gagtttagcg
     1141 ccgccctgct gcacctggca aagaggagag gcgtgcacaa cgtgaatgag gtggaggagg
     1201 acaccggcaa cgagctgtcc acaaaggagc agatcagccg caattccaag gccctggagg
     1261 agaagtatgt ggccgagctg cagctggagc ggctgaagaa ggatggcgag gtgaggggct
     1321 ccatcaatcg cttcaagacc tctgactacg tgaaggaggc caagcagctg ctgaaggtgc
     1381 agaaggccta ccaccagctg gatcagtcct ttatcgatac atatatcgac ctgctggaga
     1441 caaggcgcac atactatgag ggaccaggag agggctctcc cttcggctgg aaggacatca
     1501 aggagtggta cgagatgctg atgggccact gcacctattt tccagaggag ctgagaagcg
     1561 tgaagtacgc ctataacgcc gatctgtaca acgccctgaa tgacctgaac aacctggtca
     1621 tcaccaggga tgagaacgag aagctggagt actatgagaa gttccagatc atcgagaacg
     1681 tgttcaagca gaagaagaag cctacactga agcagatcgc caaggagatc ctggtgaacg
     1741 aggaggacat caagggctac cgcgtgacct ccacaggcaa gccagagttc accaatctga
     1801 aggtgtatca cgatatcaag gacatcacag cccggaagga gatcatcgag aacgccgagc
     1861 tgctggatca gatcgccaag atcctgacca tctatcagag ctccgaggac atccaggagg
     1921 agctgaccaa cctgaatagc gagctgacac aggaggagat cgagcagatc agcaatctga
     1981 agggctacac cggcacacac aacctgagcc tgaaggccat caatctgatc ctggatgagc
     2041 tgtggcacac aaacgacaat cagatcgcca tctttaaccg gctgaagctg gtgccaaaga
     2101 aggtggacct gtcccagcag aaggagatcc caaccacact ggtggacgat ttcatcctgt
     2161 ctcccgtggt gaagcggagc ttcatccaga gcatcaaagt gatcaacgcc atcatcaaga
     2221 agtacggcct gcccaatgat atcatcatcg agctggccag ggagaagaac tccaaggacg
     2281 cccagaagat gatcaatgag atgcagaaga ggaaccgcca gaccaatgag cggatcgagg
     2341 agatcatcag aaccacaggc aaggagaacg ccaagtacct gatcgagaag atcaagctgc
     2401 acgatatgca ggagggcaag tgtctgtatt ctctggaggc catccctctg gaggacctgc
     2461 tgaacaatcc attcaactac gaggtggatc acatcatccc ccggagcgtg agcttcgaca
     2521 attcttttaa caataaggtg ctggtgaagc aggaggagaa cagcaagaag ggcaatagga
     2581 cccctttcca gtacctgtct agctccgatt ctaagatcag ctacgagaca ttcaagaagc
     2641 acatcctgaa tctggccaag ggcaagggcc gcatcagcaa gaccaagaag gagtacctgc
     2701 tggaggagcg ggacatcaac agattctccg tgcagaagga cttcatcaac cggaatctgg
     2761 tggacaccag atacgccaca cgcggcctga tgaatctgct gcggtcttat ttcagagtga
     2821 acaatctgga tgtgaaggtg aagagcatca acggcggctt cacctccttt ctgcggagaa
     2881 agtggaagtt taagaaggag cgcaacaagg gctataagca ccacgccgag gatgccctga
     2941 tcatcgccaa tgccgacttc atctttaagg agtggaagaa gctggacaag gccaagaaag
     3001 tgatggagaa ccagatgttc gaggagaagc aggccgagag catgcccccg aagaagaaac
     3061 gtaaggtggg tagcagcggt agcccgaaaa agaagcgcaa agtgggcagc gacgcgctgg
     3121 acgatttcga tctggacatg ctgggcagcg atgcgctgga tgatttcggc ggtggcagct
     3181 ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg gccaagaggg
     3241 cacgcgatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat agagtgatcg
     3301 gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc gaaattatgg
     3361 ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc accctgtacg
     3421 gcacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg atcggccgcg
     3481 tggtgtttgg cgtgaggaac gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc
     3541 actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg
     3601 ccgccctgct gtgctatttc tttcggatgc ctagacaggt gttcaatgct cagaagaagg
     3661 cccagagctc caccgacggc ggtagcggcg gtagcggtgg tagcggcggc agcggtggta
     3721 gcggtggcag cggtggtccc gagatcgaga cagagcagga gtacaaggag attttcatca
     3781 cacctcacca gatcaagcac atcaaggact tcaaggacta caagtattct cacagggtgg
     3841 ataagaagcc caaccgcgag ctgatcaatg acaccctgta tagcacacgg aaggacgata
     3901 agggcaatac cctgatcgtg aacaatctga acggcctgta cgacaaggat aatgacaagc
     3961 tgaagaagct gatcaacaag tctcccgaga agctgctgat gtaccaccac gatcctcaga
     4021 catatcagaa gctgaagctg atcatggagc agtacggcga cgagaagaac ccactgtata
     4081 agtactatga ggagacaggc aactacctga caaagtatag caagaaggat aatggccccg
     4141 tgatcaagaa gatcaagtac tatggcaaca agctgaatgc ccacctggac atcaccgacg
     4201 attaccctaa ctctcgcaat aaggtggtga agctgagcct gaagccatac cggttcgacg
     4261 tgtacctgga caacggcgtg tataagtttg tgacagtgaa gaatctggat gtgatcaaga
     4321 aggagaacta ctatgaggtg aacagcaagt gctacgagga ggccaagaag ctgaagaaga
     4381 tcagcaacca ggccgagttc atcgcctctt tttacaacaa tgacctgatc aagatcaatg
     4441 gcgagctgta tagagtgatc ggcgtgaaca atgatctgct gaacagaatc gaagtgaata
     4501 tgatcgacat cacctacagg gagtatctgg agaacatgaa tgataagagg ccccctcgca
     4561 tcatcaagac catcgcctct aagacacaga gcatcaagaa gtacagcaca gacatcctgg
     4621 ggaacctgta tgaagtcaag agcaagaaac atcctcagat tatcaagaaa ggctctggtg
     4681 gttctaagcg acctgccgcc acaaagaagg ctggacaggc taagaagaag aaagattaca
     4741 aagacgatga cgataaggga tccggcgcaa caaacttctc tctgctgaaa caagccggag
     4801 atgtcgaaga gaatcctgga ccgatggcca agcctttgtc tcaagaagaa tccaccctca
     4861 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4921 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     4981 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5041 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5101 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5161 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     5221 aagaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
     5281 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5341 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5401 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5461 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5521 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6001 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     6301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     6361 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     6421 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     6481 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6541 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6601 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6661 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6721 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6781 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6841 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6901 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6961 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7021 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7081 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7141 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7201 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     7261 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7501 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7561 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7621 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7681 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     9721 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     9781 atctgct
//
