LOCUS       sequence-267824-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (Q731 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 Q731 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..796
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        777..795
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3014..3034
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3050..3070
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4685..4732
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4733..4756
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4766..4822
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4823..5221
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5292..5309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5298..5522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5568..5996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5655..5674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5865..5886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6005..6025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6010..6339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6190..6325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6252..6271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6406..7200
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6460..6479)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7070..7089
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7374..7495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7411..7430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7465..7484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7544..7560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7544..7560)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7557..7579)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7568..7584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7592..7598,7599..7616,7617..7622))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7637..7658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7775..7792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7946..8534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8026..8045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8705..9496,9497..9565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9328..9347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9566..9670)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9745..9764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
       61 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      121 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      781 acgactcact atagggagac ccaagctggc tagcgccacc atgaagcgaa attacattct
      841 ggggctggcc attggcatta catcagtggg ctatggcatc attgactacg agacaaggga
      901 cgtgatcgac gccggcgtga gactgttcaa ggaggccaac gtggagaaca atgagggccg
      961 gagatccaag aggggagcaa ggcgcctgaa gcggagaagg cgccacagaa tccagagagt
     1021 gaagaagctg ctgttcgatt acaacctgct gaccgaccac tccgagctgt ctggcatcaa
     1081 tccttatgag gccagagtga agggcctgtc ccagaagctg tctgaggagg agtttagcgc
     1141 cgccctgctg cacctggcaa agaggagagg cgtgcacaac gtgaatgagg tggaggagga
     1201 caccggcaac gagctgtcca caaaggagca gatcagccgc aattccaagg ccctggagga
     1261 gaagtatgtg gccgagctgc agctggagcg gctgaagaag gatggcgagg tgaggggctc
     1321 catcaatcgc ttcaagacct ctgactacgt gaaggaggcc aagcagctgc tgaaggtgca
     1381 gaaggcctac caccagctgg atcagtcctt tatcgataca tatatcgacc tgctggagac
     1441 aaggcgcaca tactatgagg gaccaggaga gggctctccc ttcggctgga aggacatcaa
     1501 ggagtggtac gagatgctga tgggccactg cacctatttt ccagaggagc tgagaagcgt
     1561 gaagtacgcc tataacgccg atctgtacaa cgccctgaat gacctgaaca acctggtcat
     1621 caccagggat gagaacgaga agctggagta ctatgagaag ttccagatca tcgagaacgt
     1681 gttcaagcag aagaagaagc ctacactgaa gcagatcgcc aaggagatcc tggtgaacga
     1741 ggaggacatc aagggctacc gcgtgacctc cacaggcaag ccagagttca ccaatctgaa
     1801 ggtgtatcac gatatcaagg acatcacagc ccggaaggag atcatcgaga acgccgagct
     1861 gctggatcag atcgccaaga tcctgaccat ctatcagagc tccgaggaca tccaggagga
     1921 gctgaccaac ctgaatagcg agctgacaca ggaggagatc gagcagatca gcaatctgaa
     1981 gggctacacc ggcacacaca acctgagcct gaaggccatc aatctgatcc tggatgagct
     2041 gtggcacaca aacgacaatc agatcgccat ctttaaccgg ctgaagctgg tgccaaagaa
     2101 ggtggacctg tcccagcaga aggagatccc aaccacactg gtggacgatt tcatcctgtc
     2161 tcccgtggtg aagcggagct tcatccagag catcaaagtg atcaacgcca tcatcaagaa
     2221 gtacggcctg cccaatgata tcatcatcga gctggccagg gagaagaact ccaaggacgc
     2281 ccagaagatg atcaatgaga tgcagaagag gaaccgccag accaatgagc ggatcgagga
     2341 gatcatcaga accacaggca aggagaacgc caagtacctg atcgagaaga tcaagctgca
     2401 cgatatgcag gagggcaagt gtctgtattc tctggaggcc atccctctgg aggacctgct
     2461 gaacaatcca ttcaactacg aggtggatca catcatcccc cggagcgtga gcttcgacaa
     2521 ttcttttaac aataaggtgc tggtgaagca ggaggagaac agcaagaagg gcaataggac
     2581 ccctttccag tacctgtcta gctccgattc taagatcagc tacgagacat tcaagaagca
     2641 catcctgaat ctggccaagg gcaagggccg catcagcaag accaagaagg agtacctgct
     2701 ggaggagcgg gacatcaaca gattctccgt gcagaaggac ttcatcaacc ggaatctggt
     2761 ggacaccaga tacgccacac gcggcctgat gaatctgctg cggtcttatt tcagagtgaa
     2821 caatctggat gtgaaggtga agagcatcaa cggcggcttc acctcctttc tgcggagaaa
     2881 gtggaagttt aagaaggagc gcaacaaggg ctataagcac cacgccgagg atgccctgat
     2941 catcgccaat gccgacttca tctttaagga gtggaagaag ctggacaagg ccaagaaagt
     3001 gatggagaac cagccgaaga agaaacgtaa ggtgggtagc agcggtagcc cgaaaaagaa
     3061 gcgcaaagtg ggcagcgacg cgctggacga tttcgatctg gacatgctgg gcagcgatgc
     3121 gctggatgat ttcggcggtg gcagctctga ggtggagttt tcccacgagt actggatgag
     3181 acatgccctg accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt
     3241 gctggtgctg aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga
     3301 cccaacagcc catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta
     3361 cagactgatt gacgccaccc tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc
     3421 catgatccac tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc
     3481 cgcaggctcc ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac
     3541 cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag
     3601 acaggtgttc aatgctcaga agaaggccca gagctccacc gacggcggta gcggcggtag
     3661 cggtggtagc ggcggcagcg gtggtagcgg tggcagcggt ggtcagatgt tcgaggagaa
     3721 gcaggccgag agcatgcccg agatcgagac agagcaggag tacaaggaga ttttcatcac
     3781 acctcaccag atcaagcaca tcaaggactt caaggactac aagtattctc acagggtgga
     3841 taagaagccc aaccgcgagc tgatcaatga caccctgtat agcacacgga aggacgataa
     3901 gggcaatacc ctgatcgtga acaatctgaa cggcctgtac gacaaggata atgacaagct
     3961 gaagaagctg atcaacaagt ctcccgagaa gctgctgatg taccaccacg atcctcagac
     4021 atatcagaag ctgaagctga tcatggagca gtacggcgac gagaagaacc cactgtataa
     4081 gtactatgag gagacaggca actacctgac aaagtatagc aagaaggata atggccccgt
     4141 gatcaagaag atcaagtact atggcaacaa gctgaatgcc cacctggaca tcaccgacga
     4201 ttaccctaac tctcgcaata aggtggtgaa gctgagcctg aagccatacc ggttcgacgt
     4261 gtacctggac aacggcgtgt ataagtttgt gacagtgaag aatctggatg tgatcaagaa
     4321 ggagaactac tatgaggtga acagcaagtg ctacgaggag gccaagaagc tgaagaagat
     4381 cagcaaccag gccgagttca tcgcctcttt ttacaacaat gacctgatca agatcaatgg
     4441 cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg aacagaatcg aagtgaatat
     4501 gatcgacatc acctacaggg agtatctgga gaacatgaat gataagaggc cccctcgcat
     4561 catcaagacc atcgcctcta agacacagag catcaagaag tacagcacag acatcctggg
     4621 gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt atcaagaaag gctctggtgg
     4681 ttctaagcga cctgccgcca caaagaaggc tggacaggct aagaagaaga aagattacaa
     4741 agacgatgac gataagggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga
     4801 tgtcgaagag aatcctggac cgatggccaa gcctttgtct caagaagaat ccaccctcat
     4861 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     4921 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     4981 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5041 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5101 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5161 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5221 agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     5281 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5341 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5401 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5461 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5521 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     5581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5641 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5761 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5881 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5941 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     6001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     6061 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     6121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     6181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     6241 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     6301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     6361 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     6421 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6481 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6541 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     6601 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6661 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6721 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6781 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6841 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6901 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6961 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     7021 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     7081 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     7141 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     7201 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     7261 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     7321 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     7381 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7441 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7501 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     7561 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7621 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7681 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7741 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7801 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7861 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7921 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7981 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8041 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8101 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8161 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8221 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8281 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8341 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8401 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8461 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8641 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     9721 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9781 tctgctc
//
