LOCUS       sequence-267825-        9485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EBFP with an upstream T7 promoter in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, 
            Marshall JL, Chen F
  TITLE     Efficient, continuous mutagenesis in human cells using a 
            pseudo-random DNA editor.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 
            10.1038/s41587-019-0331-8.
  PUBMED    31844291
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 
            10.1038/s41587-019-0331-8."
COMMENT     Sequence Label: pLX - T7pro - EBFP
FEATURES             Location/Qualifiers
     source          1..9485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1294..1312
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1313..1315,1316..1318,1319..2032)
                     /codon_start=1
                     /product="enhanced blue variant of GFP"
                     /label=EBFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1358..1379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1619..1638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1966..1987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2083..2671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2136..2156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2554..2565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2743..2976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3054..3175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3091..3110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3145..3164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3215..3350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(3371..3389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3399..3416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3399..3415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3408..3430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3557..4012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3644..3663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3854..3875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4038..4142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4143..4211,4212..5003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4361..4380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5174..5762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5663..5682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5916..5933
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6050..6071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6086..6091,6092..6109,6110..6116)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6124..6140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6129..6151
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6148..6164
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6148..6164
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6183..6203
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6185..6203
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6231..6457
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6458..6638
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6685..6810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7303..7536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7721..7765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7996..8206
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8018..8214
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8065..8200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8236..9255
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    9326..9443
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcataatacg actcactata ggatggtgag
     1321 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1381 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1441 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1501 caccctgacc cacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1561 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1621 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1681 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1741 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1801 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     1861 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     1921 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     1981 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gatagtaatg
     2041 aactagtacc ggttaagtcg acaatcaacg cgttaagtcg acaatcaacc tctggattac
     2101 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2161 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2221 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2281 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2341 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2401 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2461 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2521 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2581 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2641 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     2701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     2761 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     2821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     3001 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     3061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3181 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3301 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3361 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3541 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3601 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3661 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3721 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3781 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3841 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3901 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     3961 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4021 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4081 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4141 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4201 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4261 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4321 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4381 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4441 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4501 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4561 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4621 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4681 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4741 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4801 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4861 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4921 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4981 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5041 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5101 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6061 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6121 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6181 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6241 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6301 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6361 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6421 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7141 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7921 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     7981 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     8041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     8221 tctagaggta ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg
     8281 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct ccgagggcga agaatctcgg
     8341 gctttcagct tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat
     8401 ggtttctaca aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc
     8461 gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca
     8521 cagggtgtca ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc
     8581 gccgaggcca tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca
     8641 ttcggacctc aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct
     8701 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc
     8761 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     8821 cacgccgatt tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt
     8881 gactggagcg aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     8941 aggccctggt tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag
     9001 cttgcaggat ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat
     9061 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca
     9121 atcgtccggt ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc
     9181 gtctggaccg atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact
     9241 cgtcctaggg caaaggatct gcagtaatga ggcgcgccgt taacgaattc tagatcttga
     9301 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9361 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9421 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9481 gccgg
//
