LOCUS       sequence-267826-        6982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encoding Marburg virus NP gene under control of the AG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Schmidt KM, Schumann M, Olejnik J, Krahling V, Muhlberger E
  TITLE     Recombinant Marburg virus expressing EGFP allows rapid screening of 
            virus growth and real-time visualization of virus spread.
  JOURNAL   J Infect Dis. 2011 Nov;204 Suppl 3:S861-70. doi: 
            10.1093/infdis/jir308.
  PUBMED    21987762
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Infect 
            Dis."; date: "2011-11"; volume: "204 Suppl 3:S861-70. doi"; pages: "
            10.1093/infdis/jir308"
COMMENT     Sequence Label: pCAGGS_NP_MARV
FEATURES             Location/Qualifiers
     source          1..6982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(582..650,651..1442)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(800..819)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1613..2201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2102..2121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2355..2372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    2440..2574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2464..2483)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2518..2537
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2580..2775)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2594..2729
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    2834..2855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2870..2875,2876..2893,2894..2900)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2908..2924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2913..2935
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2932..2948
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2932..2948
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3291..3310
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3309..3364
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3410..3429
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     complement(5791..5810)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          5818..6834
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(5872..5894)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 caaaattggg ggtggggagg ggggggagat ggggagagtg aagcagaacg tggggctcac
       61 ctcgaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
      121 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
      181 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
      241 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
      301 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
      361 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
      421 ttaataacta gtcaataatc aatgtcgacc caggtggcac ttttcgggga aatgtgcgcg
      481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
      541 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
      601 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
      661 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
      721 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
      781 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
      841 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
      901 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
      961 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     1021 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     1081 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     1141 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     1201 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     1261 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     1321 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     1381 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     1441 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1501 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1561 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1621 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1681 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1741 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1801 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1861 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1921 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1981 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2041 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2101 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2161 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2221 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2281 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2341 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     2401 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcgg atccagacat gataagatac
     2461 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2521 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttagcttt
     2581 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     2641 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     2701 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     2761 gactaattga gatgcggatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2821 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2881 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2941 gctatgacca tgattacgcc aagcttgggc tgcaggtcga gggatctcca taagagaaga
     3001 gggacagcta tgactgggag tagtcaggag aggaggaaaa atctggctag taaaacatgt
     3061 aaggaaaatt ttagggatgt taaagaaaaa aataacacaa aacaaaatat aaaaaaaatc
     3121 taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg ctgtttcata
     3181 tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg ggcatatgtt
     3241 gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct cccatatgtc
     3301 cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat aaatttcctt
     3361 tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt tttggcagag
     3421 ggaaaaagat ctgctagcta attaagagct cgcggccgcc ccgggggtac cctcgaggaa
     3481 ttcgaccacc atagtatgcc cagtccttgc ccgtgattgt tatttgatgg cttatgttgt
     3541 ctcacacaat agtaacaagt aataggatac taaaaggtgg ttcaaatatt caaataaatt
     3601 tgattgctgt gatctgagga tgagtcatat caaaatagat aagtgagtaa ccgtgctgtg
     3661 ttatctggac tacaagttca tcgcaacatg tctcctttca tcaaatgaca tgtcgggcca
     3721 atctgcttga agctccttag ctgagacagg attttggtat tcctcaacga gggctgttat
     3781 aagtgactct ggaggatttg tttgcagaag atcattagga taaaggaaag ttctaccctt
     3841 ctttgtcacc actctttgtg gccaattctg ctgattatcc tcatcatttg tgaaatccgg
     3901 cgacaattca taggcttccc tcatccttgt gtcttcctga ggagcagatg gtgaagaagg
     3961 tgatcttata ggttctaaga tatcaccatt tacatcacca atactgccga agggatcctg
     4021 agggtttgct gctgggtgct gtattgggtc ctgtcttttt tcctgagcgt acaacggagg
     4081 aggtggaaga tcaactcctt gttcattgtc ggatcctggt tggtcgatgc tttcttgaga
     4141 gtcagttgtg tattccgatt catcatcctc ttgaacagga ggcaattctt gatatctcag
     4201 aaactgtttc ttaatcggat ttgtggattc atcttcctgc tttccttggc tgttattgag
     4261 gtcttgggat tgtcttggcg gttcaggaat cccttgaaat tctctcgatg ttgtgcccgg
     4321 gatcatgaca ctgtcatcaa gagtatcctc gtcctcattc agcagtgcaa atggatcatt
     4381 caagtcaaca aagctacttg aagaagagct atcttctgta gattcatgct caatcttgtc
     4441 gtcaactggg ggaggcagag cagttggtcg attcatgggc ctggcttgaa ccgttacttc
     4501 cacaggtgta gggtcattca aaaacgactg tgacacccga ttctgtgatt gcttaaatcc
     4561 ctgatcttcc acaatattgt tttcaatttc tgcagcgaga cgagctaatt tttctcgttt
     4621 ctggctgagg acggctagtg tctgactgtg tgtgatttca gttttctgaa ggtggaattg
     4681 ttctaatatc ttcctttcct cgtcatcctc ggcaatagct tgaatttcct gatgttcatg
     4741 tcgcctttgt agttttactt ccgcatcatg tgccgcctct cgtagttgtt gatattgttc
     4801 gccaacattg acaccagcca atgtactgcc gtgtgctgtt gccacaccca gcgcaattgc
     4861 tgaaagctga ggatagagtc catgttcgag gttgttaatc cctgataaat tcagaacccg
     4921 tgcaaatggt gcgtattccc catgtcgggc taggttgctc aacgcctgct tgaaactagc
     4981 aacttcattt tttactttgg aggtccgcac caaaggatgt agtgtcaccc ctgaatcagt
     5041 tttttgcaag atgaactcga gaactgtttt cacgataaga agtcctgaga atctagtttg
     5101 acctactgaa ttactaatga tactgtcata ggcatcatga cctgtcacca aatttactcc
     5161 ttgatgaatc aacacaaact ttaaaatgaa gctggacctc aaaatcccga aaattacttt
     5221 catgtggcct gtggtaagcc aatgattagg gtatgtgacg atcccctgtt cttgatgcac
     5281 tgttacttgt cttaaagcct tttcgatact agctcggtct ccgacgacaa gttttgggag
     5341 gaaaagactg caaaatgata aaaagagccc aattctcccc ctctgagatt cagtactttc
     5401 caatgtctta agggctagaa ttaaagggct gtaatgaggt tcattaggaa tcacatccag
     5461 aaagcgggtt gcatctgcat tcttgatgac atcaaattca tagcccgcat cacgtaagta
     5521 cttcgcgata ggacttgtgt tgaatttatc cttatcagaa ttatagtaat gctcaacaca
     5581 caacgtcagc aaaccccctt ctaggagatc tccaagatca atccctgagt ttattgcatc
     5641 tattatctga ttacagatac taacctgatg atttgtgtca aataatatca ctttcttatt
     5701 acgaacatga ggggcagtgg gttttgtacc caactccaac aaactgtgta aatccatatc
     5761 ttcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg
     5821 taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg
     5881 ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca
     5941 gccccgaggc tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc
     6001 gcccatttcc ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg
     6061 gcggcgcctc ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct
     6121 ctcgcacgat taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agccgccggc
     6181 gctccggggg ccgccgcgcc cctcccccga gccctccccg gcccgaggcg gccccgcccc
     6241 gcccggcacc cccacctgcc gccacccccc gcccggcacg gcgagccccg cgccacgccc
     6301 cgtacggagc cccgcacccg aagccgggcc gtgctcagca actcggggag gggggtgcag
     6361 ggggggttgc agcccgaccg acgcgcccac accccctgct caccccccca cgcacacacc
     6421 ccgcacgcag cctttgttcc cctcgcagcc ccccccgcac cgcggggcac cgcccccggc
     6481 cgcgctcccc tcgcgcacac tgcggagcgc acaaagcccc gcgccgcgcc cgcagcgctc
     6541 acagccgccg ggcagcgcgg agccgcacgc ggcgctcccc acgcacacac acacgcacgc
     6601 accccccgag ccgctccccc cgcacaaagg gccctcccgg agcccctcaa ggctttcacg
     6661 cagccacaga aaagaaacaa gccgtcatta aaccaagcgc taattacagc ccggaggaga
     6721 agggccgtcc cgcccgctca cctgtgggag taacgcggtc agtcagagcc ggggcgggcg
     6781 gcgcgaggcg gcggcggagc ggggcacggg gcgaaggcag cgcgcagcga ctcccgcccg
     6841 ccgcgcgctt cgctttttat agggccgccg ccgccgccgc ctcgccataa aaggaaactt
     6901 tcggagcgcg ccgctctgat tggctgccgc cgcacctctc cgcctcgccc cgccccgccc
     6961 ctcgccccgc cccgccccgc ct
//
