LOCUS       sequence-267827-       12284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF13B 442-1843.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF13B
FEATURES             Location/Qualifiers
     source          1..12284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1309..1311,1312..1314,1315..2022)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1354..1375)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1615..1634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1962..1983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3023..3032
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7703..7837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7740..7759)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7794..7813
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7868..7884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7868..7884)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7881..7903)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7892..7908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7916..7922,7923..7940,7941..7946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7961..7982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8099..8116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8270..8858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8350..8369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9029..9820,9821..9889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9652..9671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9890..9994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10062..10080
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10118..10140)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10240..10259
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10453..10475
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10467..10484
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10468..10484
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccta ggcctaccat ggtgagcaag
     1321 ggcggggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acatgacgcg tggaggagct
     2041 ggaataggga tgggagactc caaagtgaaa gtggcggtac gggtccggcc aatgaaccga
     2101 cgagaaattg acttacatac caaatgtgtg gtggatgttg aagcaaacaa agttattttg
     2161 aatcctataa atacaaatct ttccaaagga gatgcccggg gccagccaaa gatatttgcc
     2221 tatgatcatt gtttctggtc catggatgag tctgtccgag agaagtatgc aggtcaagat
     2281 gatgttttca agtgccttgg ggagaacatt cttcagaatg catttgatgg ctacaatgcc
     2341 tgtatctttg cctatgggca gacagggtct ggaaaatctt acactatgat gggaacagcc
     2401 gaccaaccgg ggttaatccc acgtctctgc agtggactct ttgaacgaac ccagaaagaa
     2461 gagaatgaag agcagagttt taaagtcgaa gtgtcgtaca tggaaattta taatgaaaaa
     2521 gtccgagacc ttcttgaccc caaaggaagc cgacaaacgt tgaaagtgag agagcacagt
     2581 gtgttgggac cctacgtgga tggactttct aaactggctg tcacaagcta caaggatata
     2641 gagtctctga tgtctgaggg caacaagtct cggacagttg ctgcaaccaa catgaatgaa
     2701 gaaagtagcc gttcccatgc agtcttcaaa attaccctga cacatactct gtatgatgtg
     2761 aagtctggga catctggaga gaaagtgggc aagctgagct tagtggattt agctggcagt
     2821 gaacgagcca cgaaaactgg agctgcgggc gacaggctga aggagggcag caacatcaac
     2881 aagtccctca caaccctggg tctggttatc tctgccctag ccgatcaggg tgctggcaaa
     2941 aacaaaaata aatttgttcc ctatcgagac tcagttctca catggctgct caaggacagt
     3001 ctgggaggta atagcaagac cgccatggtg gccactgtga gcccagctgc tgataactat
     3061 gatgaaacac tttcaaccct gaggtatgca gatcgagcaa agcacatcat caaccatgct
     3121 gtggtgaatg aggatcctaa tgctcggatt atccgagatc tccgtgaaga ggtggagaag
     3181 ctccgtgagc agctgactaa ggcagaggca atgaaatctc cagagctgaa agatcgcctg
     3241 gaagaatctg agaaactcat ccaggaaatg actgtgacct gggaggagaa actaaggaaa
     3301 accgaggaga ttgcgcagga gcggcagaag cagcttgaga gccttggcat atctcttcag
     3361 acttctggaa tcaaagttgg ggatgacaaa tgcttcctgg ttaacttgaa tgcggaccca
     3421 gctctgaatg aactcctggt gtactactta aaggagcaca cgctgatagg gtcagccaat
     3481 tctcaggaca tccagctctg tgggatgggc attctccctg agcatggtat catagacatc
     3541 atgcccgaag gccaggtcat gctgactcct cagaagaata ccagaacatt tgtcaatggg
     3601 tcttctgtct ccagccctat ccagctacac catggggaca ggatactatg gggaaacaac
     3661 catttcttca gactcaatct gccaaaaaag aaaaagaaag cagagcgaga ggacgaggag
     3721 cgtgaggcat ctctgaaaaa tgacagcagt tctgagcagc tggatgccga cggcgactcc
     3781 tccagtgagg tgtccagcga aatcaacttc aactttgagt atgcacagat ggaggtgacc
     3841 atgaaggctc tgggcagcaa tgacccaatg cagtccatat tgagcagcct agaacagcag
     3901 catgaggagg agaagcggtc tgctctcgag cgccagaggc tcatgtacga acacgaactg
     3961 gaacagctcc gaagaaggct gtctccggag aggcagaatt gtcgaggtgt ggaccggctt
     4021 tccttccatt cacccagtgc ccagcagcgc ttgagacagt gggctgagga gagagaagca
     4081 acattgaata acagcttgat gaggctgagg gagcagattg taaaggccaa cctgctggta
     4141 cgggaagcta gttacatcgc agaggagctg gataagagaa ccgagtataa agttaccctc
     4201 cagattccag cctccagtct ggatgccaat aggaagcgag gctctctcct cagtgaacct
     4261 gccatccagg tgagaagaaa agggaaaggg aagcagattt ggtctttgga aaagctggaa
     4321 aacaggcttt tggatatgag agacctctac caggagtgga aagaatgtga agaagacagc
     4381 ccggtttccc gatcctactt caagcgtgct gacccattct atgatgagca ggaaaatcat
     4441 agtctcatcg gtgtggccaa tgtcttcctg gagaccctgt tttatgatgt gaagttacaa
     4501 tatgctgtgc cgatcatcaa ccagaaggga gaggtggcag gccggctgca tgtggaggtg
     4561 atgcggctca gtggggccat tggggagagg attgcaggag gagacgatcc tacagaggtc
     4621 agctctgaga aggaagtcca ggagaacagg ctggtctgca tggttaaaat actccaggcc
     4681 acagggttgc ctcagcatct gtgccacttt gtcttctgca aatatgactt ctgggatcag
     4741 caggagccag tgaccgttgc tcccgaagtg gacacctctt catctcccac cagcaaggag
     4801 ccacagtgta tggttgtgtt cgatcattgc agcgagttca gtgttaacat cacagaagac
     4861 tttattgaat atctctcaga aggggccctg gccattgaag tgtatggaca taaaatgaat
     4921 gatccccgga aaaacccagc cctgtgggat ttgggaatca tccaagcaaa aacaaggagt
     4981 cttcgggaca ggtggagtga agtcaccaga aagttggaat tctgggttca gatcttggaa
     5041 cagaatgaga atggcgaata ctgccctgtg gaagtaattg ctgcaaaaga tgtcccaaca
     5101 ggggggatct tccagctccg acaggggcag tctcggcgag ttcaagttga agtgaagtca
     5161 gtgcaggagt cagggacttt gccactgatg gaagagtgca tattgtcggt tggcattggc
     5221 tgtgtgaaag ttagaccgct caggtcccct aagacccacg agaacatcca tgaggaagag
     5281 gaggacatgg acagctacca ggatagggat ttagagcggc ttcgtagaaa atggctcaat
     5341 gcattaacca aacgtcaaga atacttggat caacaactgc agaagcttgt cagtaaacat
     5401 gacaaaactg aggatgatgc tgaccgagag gcacagctct tggagatgcg gctgacactc
     5461 acagaggaaa gaaatgcggt gatggtgccg tctgctggga gtggcattcc gggggcaccg
     5521 gcagaatgga ccccagtgcc tgggatggag gcccacattc ctgttgtatt tcttgactta
     5581 aatgcggatg atttcagctc tcaggataac cttgatgacc cagaggccgg atgggatgct
     5641 accctgacta gggaagagga ggaagagttc tttgagttgc agattgtgaa gcatcacgat
     5701 ggagaggtga aggcagaggc ctcctgggac tctgcagtgc acaactgtcc tcagctcagt
     5761 aaaggcacac ctgctgacga gcgagtgttc ctcatcctga gagtggcagt ccagctcagc
     5821 caccctgcag acatgcaact ggtcctacgc aagaggattt gtgtccatgt tcatgggcgc
     5881 cagggctttg ctcaaagtct cctcaaaaag atgactcatc ggagctctat ccctggctgt
     5941 ggagtgacct ttgaaattgt ctccaatatt ccagaggatg cccagggagt ggaggagcga
     6001 gaggcactgg caaggatggc tgctaacgtg gaaaacgcag cttctgctga ctccgaggcc
     6061 tgtattgaga agtacttgag gagtgtgctg gctgtggaaa acctcctgac cctagatcgc
     6121 ctgcgtcagg aagtggccgt gaaggagcag ctgacaggaa aagggaagct gaaccggagg
     6181 agtatcagct ctcctagcat gaacagattg tcaggaagcc gacaggagct tagcccatcc
     6241 cacagtctaa gcagcaacaa gggtcggtgg gaaagtcagc aggatgtgtc tcaaacacta
     6301 gtttccagag gaatagcttc aggtcctcca gctctctctg tttcacccca aaataaccag
     6361 tctcctgatc ctggactgaa cccagcctct tacttgaacc cagtgaagtc cctggtgcca
     6421 cagatgccaa agctgctgaa gtctctcttc cctgacagaa ggggcagaca ctcatctcct
     6481 cttgtacagc agcccgtgcc ccgaatcttg gtgcagccta ctttctcaga tgcccgggca
     6541 acaaggacag aggaggctca gcagggcagc cctgggccta gtggggcttt ggagtctatg
     6601 gtgaagatgg cggcccctac agtaaagatc tgcgacaaac ctgtcagagt gtcttcccct
     6661 ccatctacca tggtagttac tcagccccaa gaagggcagg atggacctcc cagccccttg
     6721 agcgaagctt caagtggcta cttctctcac agtgtctcca ctgccaccct gtcagaaacg
     6781 ctcaccttag gcctggacac cacaggcctc ggcagccaga cacctggatc cccccctgcc
     6841 ctgtgtcagg tcacccaaga gcctgagctg gctttcctct cctgcacaca gagccatcca
     6901 actgacccag aggagcccca tataccacct gcaactccta ctcaaagcac agagctggag
     6961 gtccccagac cgcctcccct ttctgacccc acacctgctg tgcccacgtc tccattcaga
     7021 atccggaaag tgcggccctc agagcttaca tccttcacag gcatgctggg gggagcctcc
     7081 tctggagcac aggaggaccc agtggtttca gaagacccga gccatgctag gggacagact
     7141 ctagggaggc tagaagtgac atctgactct gaggatgcca gcgaagttcc tgagtggctc
     7201 agagagggag agtatgttgt tgtgggcacc aacaagacgg gcattgtaag atatattgga
     7261 ccaactgatt tccaggaggg aacgtggatt ggtgtggagc tggacctgcc tgcaggaaag
     7321 aatgatggct ccattggtgg gaaacagtat ttcagatgca accctggtta cgggctgctg
     7381 gtgaggccag gccgggtccg cagggctgca ggcactgggc gcaggcgcag ttcagggctc
     7441 cagcctcagg gagccccaga ggcccgcagg agtgctacta tctcgggctc tgccaccaac
     7501 ctggcttcac tgacagctgc attggccaag ggagacagga gctacaagaa tcctgagaac
     7561 cggaagtcct gggccagcta agtctagata agtaatgatc ataatcagcc atatcacatc
     7621 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     7681 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     7741 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7801 gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag ctcgaattcg
     7861 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7921 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7981 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8041 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8101 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8161 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8221 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8281 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8341 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8401 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8461 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8521 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8581 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8641 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8701 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8761 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8821 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8881 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8941 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9001 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9061 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    10021 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    10081 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    10141 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
    10201 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
    10261 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10321 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    10381 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    10441 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt
    10501 tgcattggcc cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg
    10561 aaactgatgg tcctggctct gtggcatggc agtggcactg tgaggagccc cctaccagca
    10621 gcacacagtg gggttggcac tgccacgctc cggatgccgc gctctgatcc aaccccataa
    10681 tcaagggaac ccgaattgcc ccatcattgc ccccaccacc cccatcctgc cgggccctca
    10741 caccccacgc tgccttctgg tgacattccc cagcccaaac ccacggcttc atggctaccg
    10801 cggggcattt cccattgccg ccccattatc agctctgcac acctcccgct gtacccatgc
    10861 ctcgtggctg cccttctttg acgtataatc ttctaattaa tacccggcct tgtcaaagtg
    10921 gagcacaaac gttaattaat tccccagcag gcaggtaatt aacagtgtga ctcccttttt
    10981 gctgcgagtg gggctgatac agagagatgt ggcactatgg agcccacggg gtcctggcac
    11041 tgggtgccca cggaggtccc catgtgctgc agtgtcaccg cctccgagga gacagtattg
    11101 tccctgcggt gtccctgcag ctcagctctg tccacagggc cacctccagt ttggagggga
    11161 cacaatgcag ccccgatgca acccatcctc gcagcatccc agggacaaag accccactgc
    11221 aagaccgcac acagggctgg gtcccgctcc cctaatatct acagtgcttc ttgcatggcc
    11281 ccttaatcaa tgcagttaat cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc
    11341 tcgcagcgct gctcaccaac accgcgcacc gccccggccc agcctgcagc acgcgctgca
    11401 aacaggaaag aaacaaaata ttgcccaaat gtaggcaaag gcattcggct gccttgacct
    11461 ccgccgggcc gggccctgcc tgactcagct ccttactcag cgctcgcttc ctccctccgg
    11521 ctgccaccgc cgcagcgcac accctgacaa agagtggccc ttaacgggct ctgaggtgca
    11581 cccagcagtg cactcagcag tccaagggcc ggcctggagg tttgcaccgc tacgtgctga
    11641 cattagcatt gaacttggcc ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag
    11701 tgcagcgcgc gttgcacccg gtgccccttc ccctcccttg catcccagca ggctgcaccc
    11761 cagcaccagg cccgtgcatg catgctcctg gtgttattgc agcctggtgc atgcatgcgt
    11821 cttgatggtg cagcgctgtg catgcatcct ccttggtgtg tagcagtcta gtgcatgcat
    11881 acccctcggt gttattgctg ctctgtgcac gcacgctcat tgtatcactt catcccagtg
    11941 catgcactca cactggagcg attgctgctc ggtgcacgca cactcattgt atcactgcag
    12001 ctcagtggct gcacgcacac cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt
    12061 cgctgcagct cagtgcatgc acgctcattg cccatcgcta tccctgcctc tcctgctggc
    12121 gctccccggg aggtgacttc aaggggaccg caggaccacc tcgggggtgg ggggagggct
    12181 gcacacgcgg accccgctcc ccctccccaa caaagcactg tggaatcaaa aaggggggag
    12241 gggggatgga ggggcgcgtc acacccccgc cccacaccct cacc
//
