LOCUS       sequence-267831-       11682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rat Myo 9b (Myr 5) in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Hemkemeyer SA, Vollmer V, Schwarz V, Lohmann B, Honnert U, Taha M, 
            Schnittler HJ, Bahler M
  TITLE     Local Myo9b RhoGAP activity regulates cell motility.
  JOURNAL   J Biol Chem. 2021 Jan-Jun;296:100136. doi: 10.1074/jbc.RA120.013623.
            Epub 2020 Dec 6.
  PUBMED    33268376
REFERENCE   2  (bases 1 to 11682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2021 Jan-Jun;296:100136. doi: 10.1074/jbc.RA120.013623. Epub 
            2020 Dec 6."
COMMENT     Sequence Label: mcherry-Myo 9b
FEATURES             Location/Qualifiers
     source          1..11682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             597..1304
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(742..760)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1010..1029
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8470..8591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8507..8526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8561..8580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8598..9053)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8735..8756)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8947..8966
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9080..9184
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9186..9543
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9186..9377
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9209..9229)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9394..9529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9578..10372
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9578..10372
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9632..9651)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10242..10261
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10560..10579)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10604..10651
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10980..11568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11469..11488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg
      601 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
      661 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      721 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
      781 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
      841 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      901 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
      961 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1021 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1081 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     1201 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     1261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagatct cgagggagtg
     1321 ctcacgaggc tggcagctca ggccgaaggc ggccggccac cttccacctg cacatctacc
     1381 ctcagctgcc cagtgctggg agccagacct catgccgtgt aaccgccacc aaggacagca
     1441 caacaagcga tgtcatccga gacgtggtgg ccagtctaca cctggacggc tcgaagcact
     1501 atgtgctggt ggaggtgaag gagtcgggtg gggaggagtg ggtgctggat gccagcgact
     1561 cgcctgtgca ccgagtgctg ctgtggcctc ggcgagcgca gaaggagcac cctcgggagg
     1621 acggctacta cttcctgctg caggagcgca acgctgacgg cagcattcag tacctgcacg
     1681 tgcagctgct ggctcagccc acagccgcat gtcgcctggt ggagcgaggg ctgctgccaa
     1741 ggcctcaagc ggacttcgac gacctgtgca acctgccgga gctgaacgag gccaacctgc
     1801 tgcagagcct gaagctgcgc ttcgtgcagc agaagatcta cacgtacgcg ggcagcatcc
     1861 tggtggccat caaccccttc aagttcctgc ccatttacaa ccccaagtat gtgaagatgt
     1921 acgagaacca gcagctggga aagctggagc cgcacgtgtt cgctctggcc gatgtagcct
     1981 actacaccat gctgcgcaag cacgtgaacc agtgcatcgt catctctggt gagagcgggt
     2041 ccggcaagac gcaaagcacc aacttcctca tccactgcct cacagcgctc agccagaagg
     2101 gctacgccag cggcgtcgag aggaccatcc tgggtgcagg gcctgtgctg gaggcttttg
     2161 ggaacgccaa gacagcccac aacaacaact ccagccgctt cgggaagttc atccaagtca
     2221 actacctgga gagtggcatc gtgaggggag ctgttgtgga aaaatacctt cttgaaaagt
     2281 ctcgcctggt ttcccaggag aaggatgagc ggaactacca tgtgttttat tatctgctgc
     2341 tgggcgtcag tgaggaagag cgtcaggaat ttcagctgaa gcagcctcaa gactatttct
     2401 acctcaacca gcataacttg aatattgaag atggagaaga cctcaaacat gactttgaaa
     2461 ggcttcagca ggccatggag atggtgggct tcctacctgc cacgaagaag cagatcttct
     2521 ctgtcctctc agccatcctg tatcttggca atgtcaccta taagaagaga gccacaggcc
     2581 gggatgaagg cctggaggtc gggcccccgg aggtattgga cacactatcc cagctcctga
     2641 aggtaaagcg ggagaccctg gtggaggtcc taaccaagag aaaaacaatc acggtcaatg
     2701 acaaactcat cctgccttac agcctcagtg aggctatcac tgcacgagac tccatggcta
     2761 agtctctgta cagcgccctg tttgactgga ttgtgctgag gatcaaccac gccctcctca
     2821 acaagaagga catggaagag gctgtttctt gcttgtccat tggcgtcctg gacatattcg
     2881 gatttgagga ctttgaacgg aacagcttcg agcagttttg catcaactac gccaacgagc
     2941 agttgcagta ctacttcacc cagcacatct tcaagctgga gcaggaggag taccagggtg
     3001 agggtatctc gtggcacaac attgactaca ccgacaacgt gggctgtatc cacctcatca
     3061 gcaagaagcc cactggcctc ttctacctgc tggacgagga aagcaacttt ccccatgcca
     3121 caagccacac tctgctggcc aaattcaagc agcagcatga ggacaacaag tacttcctgg
     3181 gcacaccagt cctggagccc gccttcatca tccagcactt cgcaggcaga gtaaagtacc
     3241 agatcaagga cttccgggag aagaacatgg actacatgcg gcctgacatc gtggcactgc
     3301 taaggggcag tgacagctcc tatgtgcgcc aactcatcgg catggacccg gtagctgtgt
     3361 tccgctgggc tgtattacgg gcagccatca gggccatggc tgtgctgcgg gaggctgggc
     3421 gcctgcgtgc agagagagca gagaaagcag aagcaggtgt aagtagtcct gtcattcgaa
     3481 gtcacgtgga agagctgcca agaggagcca acaccccttc agagaaactg taccgcgatt
     3541 tgcataacca aatcatcaag agcctcaaag gactgccatg gcagggcgag gacccgcgga
     3601 ggcttctcca gtccctcagt cggctccaga agccccgcac cttcttcctg aagagtaaag
     3661 gtatcaaaca aaagcagatc attcccaaga acctgctgga ctctaagtcc ctgaggctca
     3721 tcatcagcat gacgctgcat gaccgaacta ccaagtcact gctgcacctg cacaagaaga
     3781 agaagccgcc cagcatcagt gcacagttcc agacatctct taacaagctg ttggaggcgc
     3841 tgggcaaggc tgagcccttc ttcatccgct gcattcgctc caatgccgag aagaaggagc
     3901 tctgctttga tgatgagctg gtgcttcagc aactgcgcta cacaggcatg ctggagaccg
     3961 tgcgcatccg gcgctctggc tacagcgcca agtacacctt ccaggacttc acggagcagt
     4021 tccaggtgct gctgcccaag gatgtccagc cctgtaggga ggccattgct gccctgttgg
     4081 agaagctgca ggtggacagg cagaactacc agatcgggaa gacgaaggtc ttcctgaagg
     4141 agacagagcg gcaggccctg caggagaggc tgcatggtga ggtcttacgc aggatcctgc
     4201 tgctgcagag ttggttccgg atggtgctgg aacgcaggca ctttgtgcag atgaagcatg
     4261 ctgccctgac catccaggcc tgctggcggt cttatcgtgt gcgccgtaca ctggagagga
     4321 cgcgggcagc tgtgtatctt caggctgcct ggagaggcta cctgcagaga caggcctacc
     4381 accaccagag gcatagcatc atccgcctgc agagcctctg ccgtggccac ctacagcgca
     4441 ggagcttcag ccagatgatg ttagagaagc agaaggcaga gcaagccagg gagactgcag
     4501 gagcagagat gtcagaggga gagcccagcc ctgtggccgc tggggagcag ccgtctgagc
     4561 accctgtgga ggaccctgag agcctgggtg tggagactga gacctggatg aacagcaagt
     4621 ccccaaatgg cttgtcacct aagaaggaga tccccagccc ggagatggag accccagccc
     4681 aaaaaacagt gccagctgaa agtcatgaga aagtccccag tagccgggag aagcgagagt
     4741 cacggcggca gcgagggttg gagcatgttg aacgacagaa caaacacatc caatcctgca
     4801 gggaggagaa tagcaccctc cgagaacctt ccagaaaggc aagcctggaa acaggggaga
     4861 gcttccctga ggacacaaag gagcccagag aagatggact tgagacatgg actgagaccg
     4921 cagccccctc ttgtccaaag caggtgccga ttgtgggaga tccgcctagg agtcccagtc
     4981 ccttgcagag gcccgccagc ctggacctag acagcagggt tagcccagtg ctccccagca
     5041 gctccctgga atccccccag gatgaggaca agggtgagaa cagcaccaag gttcaggaca
     5101 agcccgagag tcccagtggc tccacccaga tccaacggta ccaacacccg gacacagagc
     5161 ggctagccac tgctgtggag atatggcgag gcaagaagct cgccagtgcc atgctgagcc
     5221 aatccctgga cctgagtgag aagccccgga ctgcaggggc agccctgact cccacagagg
     5281 agaggcgcat ctccttctcc accagtgatg tctccaagct gtccccggtc aagacttcaa
     5341 ctgaagtcga tggggatctg agcgccaaga agccagccgg ccataagaag aagtcagaag
     5401 acccatctgc tggtcccgat gcaggcctgc ccacaggctc ccagggtgac tctaaatctg
     5461 cctttaagcg actcttcctg cacaaagcca aggataagaa gcccagcctg gagggtgtgg
     5521 aggagacaga gggcagtgga gggcaggctg cacaggaggc cccggccagg aagactctag
     5581 atgtaccttc tagccagcag caccgccaca ccacaggcga gaagcctcta aaagggaaga
     5641 agaaccgaaa tcgtaaggtc ggacagatca cagtgtccga gaagtggcga gagtcggtgt
     5701 tccgtaagat cactaatgcc aacgagctca agtttctgga tgagttcctg ctcaacaagg
     5761 tgaatgacct tcgctcacag aagacaccca tcgagagctt gttcattgag gccactgagc
     5821 gtttcaggag caatatcaag accatgtatt ctgtgcctaa cgggaagatc catgtaggct
     5881 acaaggactt gatggagaac taccagatcg ttgtcagtaa cctggctgcc gagcgtgggg
     5941 agaaggacac caacctggtc ctcaatgtct tccagtcact gctggatgaa ttcactcgca
     6001 gctacaacaa gactgacttt gagccggtca agggcaaagc ccagaagaag aagaggaagc
     6061 aggagcgtgc tgtccaggaa cacaatggac atgtgtttgc cagctaccaa gtgaacatcc
     6121 cgcagtcgtg tgagcagtgt ctgtcctaca tctggctcat ggacaaggcc ctgctatgca
     6181 gtgtgtgcaa gatgacctgc cacaagaaat gtgtgcacaa gattcagagc tattgctcct
     6241 acactggacg gaggaagagt gagctgggtg ccgaaccagg ccacttcggc gtgtgtgtag
     6301 acagcctgac cagtgacaag gcctccgtgc ccattgtgct ggagaagctt ctggaacatg
     6361 tagagatgca tggcctgtat accgagggtc tttaccgcaa gtcaggcgct gccaaccgga
     6421 cacgggaatt acgccaggct ctgcagacag accctgctac agttaagctg gaggacttcc
     6481 ccatccacgc catcaccggg gtcctgaagc aatggctgcg ggagctgcct gagccactca
     6541 tgacttttgc ccagtatgga gacttcctca gggctgttga gcttccagag aagcaggagc
     6601 agctggctgc catctatgca gtcctggacc acctgcctga agccaaccac acctccctgg
     6661 agaggctcat cttccacctt gtcaaagtgg ccctgcttga agatgtgaac cgcatgtctc
     6721 cgggagctct agctatcatc ttcgcgccct gcctgcttcg ctgccctgac aactccgacc
     6781 ccctgaccag catgaaggac gtactaaaga ttaccacgtg tgtggagatg ctcatcaagg
     6841 aacagatgag gaaatacaag gtgaagatgg aggagatcaa ccacctggag gcagctgaga
     6901 gcattgcgtt ccgcaggctc tccctgctga ggcagaatgc cccgtggcct ctcaaactgg
     6961 ggttttcgtc tccctatgag ggggtccgga ccaaaagccc caggacccca gtggtccaag
     7021 acttggagga gctgggggct ctccccgagg aggcggcagg tggtgacgag gaccgggaaa
     7081 aggagattct catggagagg attcagtcca tcaaagaaga gaaggaggac atcacatatc
     7141 gactgccgga gctggaccca cggggttctg acgaggagaa ccttgactca gagacatcag
     7201 ccagcactga gagcctgctg gaggagaggg ccgtgcgggg ggccgcagaa gagtgaagac
     7261 accccggagg acccccatca tgcccatggc caacatcaag ctccctccgg gcctgccctt
     7321 gcacctgacg agctgggcac ctgctctcca ggaggctgct gttccagtga agcgccgaga
     7381 gccacctgcc cgccgacagg accaggtaca ttccgtatac atcgcccctg gagctgacct
     7441 gccatcgcag ggtacactag gacccctgga ccaccatgat gccatacttc ctggggccaa
     7501 acgcaggtat tcagaccccc ctacctactg cctgccctcc agctccagcc aagccaatgg
     7561 ctgaggacca tgactggcca tctgcgtgtt ctaaaatccc cgaactggca tcccagctgt
     7621 ggagccggcc ttcactctct gagaaggatc tagaatgaaa agttcccaga gggatgctgt
     7681 ggccagctct gtgtgttgtg gaggctgggg actgctggcc aggaccatca gagccccaac
     7741 ctgcacagca gtcccccccc cccctttgta cttttcagta actgtttctc tttttgtggt
     7801 ttacataact tttaagttca taacagcctt aatggaggac caaacttttt gtacgtgtgt
     7861 ctgagctttg atattaactg tccaccgctg taacctggac atgggcagga ctagcctgca
     7921 aagtggatat gtgggccaca cctggcgact gcactccaca gccatggaga gcaagcaagc
     7981 aagcgaacga gcggactcac aagacacctc ttacccttga atacctccca gcagcggtca
     8041 tctggccaga gaaccttcca gagattgtgg gagctgtcca cagggtcaca gctgaaggca
     8101 ggacagaatg ggaagtccct gcatcgcagg gatgctctac ccactgcctg tgggctgcaa
     8161 ggagccaggg atcggagctg tagggacaag cagtgcatgt cccgaatttc ctctgcagcc
     8221 cccgtgtcag cctgggttct taagaggctg gctctcttct caggccctac ccagtcctgg
     8281 acacaaggaa gccccaccgg aggtgtgaaa taaaagtgct tgacaagtgt ggaattcgat
     8341 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     8401 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8461 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8581 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8641 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8701 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8761 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8821 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8881 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8941 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9001 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     9061 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9181 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     9241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     9301 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9361 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9421 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9481 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9541 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     9601 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     9661 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     9721 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     9781 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     9841 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     9901 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     9961 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
    10021 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
    10081 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
    10141 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
    10201 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
    10261 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
    10321 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
    10381 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
    10441 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    10501 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
    10561 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
    10621 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    10681 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    10741 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    10801 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
    10861 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    10921 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    10981 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11041 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11101 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11161 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11221 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11281 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11341 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    11401 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    11461 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11521 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    11581 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    11641 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
