LOCUS       sequence-267833-        7782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse Myo 1g in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Olety B, Walte M, Honnert U, Schillers H, Bahler M
  TITLE     Myosin 1G (Myo1G) is a haematopoietic specific myosin that localises
            to the plasma membrane and regulates cell elasticity.
  JOURNAL   FEBS Lett. 2010 Feb 5;584(3):493-9. doi: 
            10.1016/j.febslet.2009.11.096. Epub 2009 Dec 4.
  PUBMED    19968988
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2009.11.096"; journalName: "FEBS Lett"; date: 
            "2010-02-5- 5"; volume: "584"; issue: "3"; pages: "493-9"
COMMENT     Sequence Label: GFP-Myo 1g
FEATURES             Location/Qualifiers
     source          1..7782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3290..3301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4570..4691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4607..4626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4661..4680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4698..5153)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4835..4856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5047..5066
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5180..5284
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5286..5643
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5286..5477
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5309..5329)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5494..5629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5678..6472
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5678..6472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5732..5751)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6342..6361
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6660..6679)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6704..6751
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7080..7668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7569..7588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatgctt gcagtgggca ggatggagga cgaagaaggc
     1381 cctgagtatg ggaaaccaga cttcgtgctt ttggatcaac tgaccatgga ggacttcatg
     1441 aagaacctag agctcaggtt tgagaagggc cgtatctata cctacattgg tgaggtgctc
     1501 gtatccgtga acccctacca ggaactgcca ttgtatgggc cagaggccat tgccaagtac
     1561 cagggccgcg agctctatga gcgaccacct catctttacg ccgtggccaa tgctgcttac
     1621 aaggcaatga agcgcagatc cagggacacc tgcatcgtca tctcagggga gagtggggca
     1681 gggaagacag aagccagcaa gcacatcatg cagtacattg ctgctgtcac caacccaagc
     1741 cagagggctg aggtggagag ggtgaagaat gtgctcctca agtccacctg tgtgctcgaa
     1801 gcctttggca atgcccgcac caatcgcaac cacaactcca gccgctttgg caagtacatg
     1861 gacatcaact tcgacttcaa gggggatcct gttggtggac acatccacag ctacctgctg
     1921 gagaagtcta gggttctcaa gcaacatgta ggtgagagga acttccatgc cttctaccag
     1981 ttgcttcgag gcagtgagga ccaagagctg caaggactgc atctggaaag aaatcctgct
     2041 gtgtataatt tcacgcgtca gggagctggg ctcaacatgg gtgtgcacaa tgccttggac
     2101 agtgatgaga agagccacca aggagtgatg gaggccatga ggatcatcgg cttcagtcct
     2161 gacgaggtgg agtccatcca tcgcatcctt gccgccatat tacacctggg aaacatcgag
     2221 tttgtggaga cagaggaaaa tggaccacag aaaggaggcc tggaagtggc tgatgaggcc
     2281 ctggtaggat atgtggccaa gctgacagcc actcccagag accttgttct gcgaaccctg
     2341 ctggctcgaa cagtggcttc aggaggccga gaagtcattg agaagagcca caccgtggct
     2401 gaggccagct atgcccggga tgcctgtgca aaggcgatgt accagcgact gtttgagtgg
     2461 gttgtgaaca agatcaatag catcatggaa ccccgaaacc gagaccctcg gtgtgatggc
     2521 aaggatactg tcattggggt gctggacatt tacggctttg aggtgttccc tgtcaacagc
     2581 tttgagcagt tctgcatcaa ctactgcaac gagaagctgc agcagctctt tatccagctt
     2641 atcctgaagc aagagcagga ggagtatgag cgagagggca tcgcctggca gaccatcgag
     2701 tacttcaaca acgctaccat tgtggaactt gtagagcagc cccacagagg catcctggct
     2761 gtgttagatg aagcctgcag cacggcaggc cccatcactg accgaatctt cctgcagacc
     2821 ctggacacac accaccgcca ccacccacac tattccagcc gccagctttg ccctacggac
     2881 aagaccatgg agtttggccg agacttccag atcaaacact atgcaggcga tgtcacgtac
     2941 tctgtggaag gcttcattga caagaataga gactctctct tccaggactt caaacggctg
     3001 ctgtacaata gtgtggatcc caccttgcga gccatgtggc ctgacgggca acaggacatc
     3061 acggaagtga ccaagcgtcc cctgacagcc ggcacactct ttaagaattc catggttgcc
     3121 ctggtggaaa acttggcttc caaggaaccc ttctatgtcc gctgcatcaa acccaacgaa
     3181 gacaaggtgg ctgggcggct cgatgaagcc cactgtcgtc accaggtcga atacctggga
     3241 ctattggaga atgtgagggt ccgcagggct ggctttgctt cccggcagcc ctaccctcga
     3301 ttcctgctca ggtacaagat gacctgtgag tacacgtggc ccaaccacct gctgggctct
     3361 gaccgggatg cggtgagcgc cctgctggaa cagcatgggc tgcaagggga tgtggccttt
     3421 ggccacagca agctgttcat ccgatcccca aggacgctgg tcactctgga gcagagccga
     3481 gctcgcctga ttcccatcat tgtgttattg ctgcagaagg cttggcgggg caccctggct
     3541 aggtggcact gccggcgact aagggccatc tacaccatca tgcgctggtt ccggaggcac
     3601 aaggtgcgtg ctcacctgat tgaactacag cgccggttcc aggctgcacg gcagccccca
     3661 ctctatggcc gtgaccttgt gtggcccaca cctcctgctg tgctgcagcc cttccaggac
     3721 acttgccgtg ttctcttcag caggtggcgg gcacggcagt tagtgaagaa catccctcct
     3781 tcagacatga tccagatcaa ggccaaggtg gctgctatgg gggccttgca aggattgcgg
     3841 caggactggg gttgccagcg ggcctgggct cgagactacc tgtcctctga cactgacaac
     3901 cccacagctt cccatctgtt tgctgagcaa ctaaaggcac ttcgggagaa agatggcttt
     3961 ggctctgtgc ttttctccag ccatgtgcgc aaggtgaatc gcttccgcaa gagccgggac
     4021 cgggcccttc tgctcacaga tcggtatctg tacaagctgg agcctggacg acagtaccgg
     4081 gtgatgcggg ctgtgcctct ggaggcggtg acagggctga gtgtgaccag tggaagagat
     4141 cagctggtgg tgttacatgc ccaaggctat gatgatcttg tagtgtgtct acaccgttcc
     4201 caaccaccac tggacaatcg aattggggag ctggtgggca tgctggctgc acactgccag
     4261 ggagagggac gaactctgga ggtccgtgtc tctgactgca tcccactgag ccagcgtggt
     4321 gcccggcgcc tcatttctgt ggagcccagg ccagagcagc ctgagccaga tttccaaagc
     4381 agccgtagca cctttaccct cctctggcca agccactgag tcgacggtac cgcgggcccg
     4441 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     4501 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4561 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4681 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4741 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4801 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4861 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4921 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4981 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5041 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5101 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5281 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5401 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5461 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5641 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5701 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5761 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5821 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5881 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5941 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     6001 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     6061 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     6121 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     6181 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6241 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6301 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6361 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6421 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6481 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6601 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6661 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6721 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6781 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6841 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6901 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7741 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
