LOCUS       sequence-267834-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (T128 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 T128 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     766..785
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        766..784
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1194..1214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1230..1250
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4674..4721
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4722..4745
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4755..4811
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4812..5210
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5281..5298)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5287..5511
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5557..5985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5644..5663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5854..5875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5994..6014)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5999..6328
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6179..6314
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6241..6260
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6395..7189
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6449..6468)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7059..7078
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7363..7484
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7400..7419)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7454..7473
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7533..7549)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7533..7549)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7546..7568)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7557..7573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7581..7587,7588..7605,7606..7611))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7626..7647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7764..7781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7935..8523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8015..8034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8694..9485,9486..9554))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9317..9336
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9555..9659)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9734..9753)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
       61 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
      121 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      661 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
      781 tagggagacc caagctggct agcgccacca tgaagcgaaa ttacattctg gggctggcca
      841 ttggcattac atcagtgggc tatggcatca ttgactacga gacaagggac gtgatcgacg
      901 ccggcgtgag actgttcaag gaggccaacg tggagaacaa tgagggccgg agatccaaga
      961 ggggagcaag gcgcctgaag cggagaaggc gccacagaat ccagagagtg aagaagctgc
     1021 tgttcgatta caacctgctg accgaccact ccgagctgtc tggcatcaat ccttatgagg
     1081 ccagagtgaa gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc gccctgctgc
     1141 acctggcaaa gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac accccgaaga
     1201 agaaacgtaa ggtgggtagc agcggtagcc cgaaaaagaa gcgcaaagtg ggcagcgacg
     1261 cgctggacga tttcgatctg gacatgctgg gcagcgatgc gctggatgat ttcggcggtg
     1321 gcagctctga ggtggagttt tcccacgagt actggatgag acatgccctg accctggcca
     1381 agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg aacaatagag
     1441 tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc catgccgaaa
     1501 ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt gacgccaccc
     1561 tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc catgatccac tctaggatcg
     1621 gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg
     1681 tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc ctggcagatg
     1741 aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc aatgctcaga
     1801 agaaggccca gagctccacc gacggcggta gcggcggtag cggtggtagc ggcggcagcg
     1861 gtggtagcgg tggcagcggt ggtaccggca acgagctgtc cacaaaggag cagatcagcc
     1921 gcaattccaa ggccctggag gagaagtatg tggccgagct gcagctggag cggctgaaga
     1981 aggatggcga ggtgaggggc tccatcaatc gcttcaagac ctctgactac gtgaaggagg
     2041 ccaagcagct gctgaaggtg cagaaggcct accaccagct ggatcagtcc tttatcgata
     2101 catatatcga cctgctggag acaaggcgca catactatga gggaccagga gagggctctc
     2161 ccttcggctg gaaggacatc aaggagtggt acgagatgct gatgggccac tgcacctatt
     2221 ttccagagga gctgagaagc gtgaagtacg cctataacgc cgatctgtac aacgccctga
     2281 atgacctgaa caacctggtc atcaccaggg atgagaacga gaagctggag tactatgaga
     2341 agttccagat catcgagaac gtgttcaagc agaagaagaa gcctacactg aagcagatcg
     2401 ccaaggagat cctggtgaac gaggaggaca tcaagggcta ccgcgtgacc tccacaggca
     2461 agccagagtt caccaatctg aaggtgtatc acgatatcaa ggacatcaca gcccggaagg
     2521 agatcatcga gaacgccgag ctgctggatc agatcgccaa gatcctgacc atctatcaga
     2581 gctccgagga catccaggag gagctgacca acctgaatag cgagctgaca caggaggaga
     2641 tcgagcagat cagcaatctg aagggctaca ccggcacaca caacctgagc ctgaaggcca
     2701 tcaatctgat cctggatgag ctgtggcaca caaacgacaa tcagatcgcc atctttaacc
     2761 ggctgaagct ggtgccaaag aaggtggacc tgtcccagca gaaggagatc ccaaccacac
     2821 tggtggacga tttcatcctg tctcccgtgg tgaagcggag cttcatccag agcatcaaag
     2881 tgatcaacgc catcatcaag aagtacggcc tgcccaatga tatcatcatc gagctggcca
     2941 gggagaagaa ctccaaggac gcccagaaga tgatcaatga gatgcagaag aggaaccgcc
     3001 agaccaatga gcggatcgag gagatcatca gaaccacagg caaggagaac gccaagtacc
     3061 tgatcgagaa gatcaagctg cacgatatgc aggagggcaa gtgtctgtat tctctggagg
     3121 ccatccctct ggaggacctg ctgaacaatc cattcaacta cgaggtggat cacatcatcc
     3181 cccggagcgt gagcttcgac aattctttta acaataaggt gctggtgaag caggaggaga
     3241 acagcaagaa gggcaatagg acccctttcc agtacctgtc tagctccgat tctaagatca
     3301 gctacgagac attcaagaag cacatcctga atctggccaa gggcaagggc cgcatcagca
     3361 agaccaagaa ggagtacctg ctggaggagc gggacatcaa cagattctcc gtgcagaagg
     3421 acttcatcaa ccggaatctg gtggacacca gatacgccac acgcggcctg atgaatctgc
     3481 tgcggtctta tttcagagtg aacaatctgg atgtgaaggt gaagagcatc aacggcggct
     3541 tcacctcctt tctgcggaga aagtggaagt ttaagaagga gcgcaacaag ggctataagc
     3601 accacgccga ggatgccctg atcatcgcca atgccgactt catctttaag gagtggaaga
     3661 agctggacaa ggccaagaaa gtgatggaga accagatgtt cgaggagaag caggccgaga
     3721 gcatgcccga gatcgagaca gagcaggagt acaaggagat tttcatcaca cctcaccaga
     3781 tcaagcacat caaggacttc aaggactaca agtattctca cagggtggat aagaagccca
     3841 accgcgagct gatcaatgac accctgtata gcacacggaa ggacgataag ggcaataccc
     3901 tgatcgtgaa caatctgaac ggcctgtacg acaaggataa tgacaagctg aagaagctga
     3961 tcaacaagtc tcccgagaag ctgctgatgt accaccacga tcctcagaca tatcagaagc
     4021 tgaagctgat catggagcag tacggcgacg agaagaaccc actgtataag tactatgagg
     4081 agacaggcaa ctacctgaca aagtatagca agaaggataa tggccccgtg atcaagaaga
     4141 tcaagtacta tggcaacaag ctgaatgccc acctggacat caccgacgat taccctaact
     4201 ctcgcaataa ggtggtgaag ctgagcctga agccataccg gttcgacgtg tacctggaca
     4261 acggcgtgta taagtttgtg acagtgaaga atctggatgt gatcaagaag gagaactact
     4321 atgaggtgaa cagcaagtgc tacgaggagg ccaagaagct gaagaagatc agcaaccagg
     4381 ccgagttcat cgcctctttt tacaacaatg acctgatcaa gatcaatggc gagctgtata
     4441 gagtgatcgg cgtgaacaat gatctgctga acagaatcga agtgaatatg atcgacatca
     4501 cctacaggga gtatctggag aacatgaatg ataagaggcc ccctcgcatc atcaagacca
     4561 tcgcctctaa gacacagagc atcaagaagt acagcacaga catcctgggg aacctgtatg
     4621 aagtcaagag caagaaacat cctcagatta tcaagaaagg ctctggtggt tctaagcgac
     4681 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa gacgatgacg
     4741 ataagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga
     4801 atcctggacc gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     4861 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     4921 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     4981 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5041 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5101 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5161 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattctgca
     5221 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
     5281 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5341 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5401 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5461 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5521 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6001 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6061 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6121 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6181 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6241 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6301 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6361 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6421 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6481 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6541 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     6601 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6661 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6721 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6781 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6841 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6901 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6961 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     7021 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     7081 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     7141 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     7201 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     7261 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7321 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7381 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7441 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7501 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7561 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7621 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7681 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7741 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7801 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7861 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7921 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7981 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8041 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8101 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8161 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8221 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8281 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8341 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8401 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8461 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8521 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8581 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8641 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8701 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8761 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8821 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8881 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8941 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9001 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9061 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9121 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9181 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9241 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9301 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9361 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9421 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9481 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9541 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9601 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9661 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     9721 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9781 cttgtgt
//
