LOCUS       sequence-267835-       10789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding miniABEMax (evolved Tada monomer with V82G) at the 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 miniABEMax V82G strategy1
FEATURES             Location/Qualifiers
     source          1..10789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     770..789
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        770..788
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1337..1357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1373..1393
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5657..5704
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5705..5728
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5738..5794
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6287..6304)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6293..6517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6563..6991
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6650..6669)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6860..6881
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7000..7020)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7005..7334
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7185..7320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7247..7266
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7401..8195
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7455..7474)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8065..8084
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8369..8490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8406..8425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8460..8479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8539..8555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8539..8555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8552..8574)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8563..8579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8587..8593,8594..8611,8612..8617))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8632..8653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8770..8787)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8941..9529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9021..9040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9700..10491,10492..10560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10323..10342
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10561..10665)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10740..10759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
       61 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      121 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      181 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      241 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      301 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      361 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      421 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      481 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      541 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      601 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      661 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      721 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      781 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgtc
      841 tgaggtggag ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc
      901 acgcgatgag agggaggtgc ctgtgggagc cgtgctggtg ctgaacaata gagtgatcgg
      961 cgagggctgg aacagagcca tcggcctgca cgacccaaca gcccatgccg aaattatggc
     1021 cctgagacag ggcggcctgg tcatgcagaa ctacagactg attgacgcca ccctgtacgg
     1081 cacattcgag ccttgcgtga tgtgcgccgg cgccatgatc cactctagga tcggccgcgt
     1141 ggtgtttggc gtgaggaacg caaaaaccgg cgccgcaggc tccctgatgg acgtgctgca
     1201 ctaccccggc atgaatcacc gcgtcgaaat taccgaggga atcctggcag atgaatgtgc
     1261 cgccctgctg tgctatttct ttcggatgcc tagacaggtg ttcaatgctc agaagaaggc
     1321 ccagagctcc accgacccga agaaaaagcg taaagtgggt agcagcggca gcccgaaaaa
     1381 gaaacgcaaa gtgggtagcg acgctctgga tgacttcgac ctggatatgc tgggcagcga
     1441 cgccctggat gactttggcg gtggcagcat aggcaaggca accgctaagt atttttttta
     1501 cagcaacatc atgaacttct ttaagaccga gattaccctg gccaacggtg aaatccgtaa
     1561 acgcccgctg attgaaacca acggtgaaac cggcgaaatc gtgtgggaca agggccgtga
     1621 ttttgcgacc gtgcgcaaag tgctgtccat gccgcaggtg aacattgtga agaaaaccga
     1681 ggtgcagacc ggcggtttca gcaaggaaag cattctgccg aaacgtaaca gcgacaagct
     1741 gatcgcccgc aagaaagatt gggacccgaa gaaatacggc ggtttcgata gcccgaccgt
     1801 ggcttatagc gtgctggtgg tggccaaagt ggagaagggt aaaagcaaga aactgaagag
     1861 cgtgaaagaa ctgctgggca tcaccattat ggagcgtagc agcttcgaaa agaacccgat
     1921 cgactttctg gaggcgaagg gttacaagga agtgaagaaa gatctgatca ttaagctgcc
     1981 gaaatacagc ctgtttgagc tggaaaatgg tcgtaaacgt atgctggcca gcgccggcga
     2041 gctgcaaaag ggcaacgaac tggcgctgcc gagcaaatat gtgaacttcc tgtacctggc
     2101 cagccactat gagaagctga aaggtagccc ggaggacaac gaacagaagc agctgtttgt
     2161 ggagcagcac aaacactatc tggatgagat cattgaacag atcagcgagt tcagcaagcg
     2221 tgtgattctg gcggacgcta acctggataa agtgctgagc gcctacaaca aacaccgtga
     2281 caagccgatc cgcgagcagg cggaaaacat cattcacctg ttcaccctga ccaacctggg
     2341 tgcgccggcc gcgttcaagt actttgacac caccattgat cgtaaacgct ataccagcac
     2401 caaggaggtg ctggatgcta ccctgatcca ccagagcatt accggcctgt acgaaacccg
     2461 tattgatctg agccagctgg gcggtgacgg cggtagcggc ggtagcggcg gtagcggcgg
     2521 tagcggcggt agcggcggta gcggcggtga caagaaatat agcatcggtc tggcgattgg
     2581 caccaacagc gtgggttggg ctgtgatcac cgatgagtac aaggtgccga gcaagaagtt
     2641 caaggtgctg ggcaacaccg accgccacag catcaagaaa aacctgattg gtgccctgct
     2701 gtttgatagc ggtgaaaccg ccgaggccac ccgtctgaag cgcaccgccc gtcgccgtta
     2761 cacccgtcgt aaaaaccgta tctgctacct gcaagagatt ttcagcaacg aaatggccaa
     2821 agtggacgat agcttctttc accgcctgga ggagagcttc ctggtggagg aagacaagaa
     2881 acacgagcgt cacccgatct ttggcaacat tgtggatgag gtggcttacc acgaaaagta
     2941 tccgaccatc taccacctgc gtaagaaact ggtggatagc accgacaagg ccgatctgcg
     3001 cctgatctat ctggctctgg cccacatgat caagttccgt ggtcactttc tgatcgaggg
     3061 cgacctgaac ccggataaca gcgatgtgga caagctgttc atccagctgg tgcagaccta
     3121 caaccagctg tttgaggaaa acccgattaa cgccagcggt gtggacgcga aggctatcct
     3181 gagcgcgcgt ctgagcaaaa gccgccgtct ggagaacctg atcgcgcagc tgccgggtga
     3241 aaagaaaaac ggtctgtttg gcaacctgat tgctctgagc ctgggcctga ccccgaactt
     3301 caaaagcaac tttgacctgg cggaggatgc taagctgcaa ctgagcaagg atacctatga
     3361 cgatgacctg gacaacctgc tggcgcagat cggcgaccag tacgctgatc tgttcctggc
     3421 tgccaagaac ctgagcgatg cgatcctgct gagcgacatt ctgcgtgtga acaccgagat
     3481 taccaaggcc ccgctgagcg cgagcatgat caaacgctat gatgaacacc accaggacct
     3541 gaccctgctg aaagctctgg tgcgtcagca gctgccggag aagtacaaag aaatcttctt
     3601 tgaccagagc aagaacggtt acgccggcta tattgatggc ggtgcgagcc aggaggagtt
     3661 ctacaagttt atcaaaccga ttctggagaa aatggacggc accgaggaac tgctggtgaa
     3721 gctgaaccgt gaagatctgc tgcgtaaaca gcgcaccttc gacaacggta gcatcccgca
     3781 ccagattcac ctgggtgagc tgcacgccat cctgcgccgt caggaagatt tctacccgtt
     3841 tctgaaggac aaccgtgaga agatcgaaaa aattctgacc ttccgcattc cgtactatgt
     3901 gggtccgctg gctcgtggca acagccgctt tgcctggatg acccgcaaga gcgaggaaac
     3961 catcaccccg tggaacttcg aggaagtggt ggacaaaggc gccagcgcgc agagcttcat
     4021 tgagcgtatg accaactttg ataagaacct gccgaacgaa aaagtgctgc cgaagcacag
     4081 cctgctgtac gagtatttca ccgtgtacaa cgaactgacc aaggtgaaat atgtgaccga
     4141 gggtatgcgc aagccggcct tcctgagcgg cgaacagaag aaagcgatcg tggacctgct
     4201 gtttaaaacc aaccgtaagg tgaccgtgaa gcagctgaaa gaggattatt tcaagaaaat
     4261 tgaatgcttt gacagcgtgg agatcagcgg tgtggaagat cgttttaacg cgagcctggg
     4321 cacctaccac gacctgctga aaatcattaa ggataaagac ttcctggata acgaggaaaa
     4381 cgaggacatc ctggaagata ttgtgctgac cctgaccctg tttgaggacc gtgaaatgat
     4441 cgaggaacgc ctgaagacct atgcccacct gttcgatgac aaagtgatga aacagctgaa
     4501 gcgccgtcgc tacaccggtt ggggccgtct gagccgcaaa ctgatcaacg gtattcgcga
     4561 taaacagagc ggcaagacca tcctggattt tctgaagagc gacggtttcg cgaaccgtaa
     4621 ctttatgcag ctgattcacg atgacagcct gaccttcaaa gaggacatcc agaaggcgca
     4681 ggtgagcggt cagggcgata gcctgcacga acatattgct aacctggccg gtagcccggc
     4741 catcaagaag ggcatcctgc aaaccgtgaa ggtggtggac gagctggtga aagtgatggg
     4801 tcgtcacaag ccggaaaaca tcgtgattga gatggcccgc gaaaaccaga ccacccagaa
     4861 aggccagaag aacagccgtg agcgcatgaa gcgtatcgag gaaggcatca aggaactggg
     4921 cagccagatc ctgaaggagc acccggtgga aaacacccag ctgcaaaacg agaaactgta
     4981 cctgtactac ctgcaaaacg gtcgtgacat gtatgtggat caggaactgg acattaaccg
     5041 cctgagcgat tatgacgtgg atgcgatcgt gccgcagagc ttcctgaagg atgacagcat
     5101 tgacaacaaa gtgctgaccc gtagcgataa aaaccgcggc aagagcgaca acgtgccgag
     5161 cgaggaagtg gtgaagaaaa tgaaaaacta ctggcgtcag ctgctgaacg ccaaactgat
     5221 cacccagcgt aagtttgaca acctgaccaa agctgagcgc ggcggtctga gcgaactgga
     5281 taaagccggt ttcattaagc gtcagctggt ggaaacccgc cagatcacca agcacgtggc
     5341 gcagattctg gacagccgca tgaacaccaa gtatgacgag aacgataaac tgatccgtga
     5401 agtgaaagtg atcaccctga agagcaaact ggtgagcgat tttcgtaaag acttccagtt
     5461 ttacaaggtg cgcgagatca acaactacca ccacgctcac gacgcctatc tgaacgcggt
     5521 ggtgggcacc gccctgatca agaagtaccc gaagctggag agcgagttcg tgtacggcga
     5581 ctacaaggtg tatgatgtgc gtaagatgat cgccaaaagc gagcaggaaa ttggcggcgg
     5641 cgatagcggt ggtagcaagc gtccggcggc taccaagaaa gcgggccagg ctaagaaaaa
     5701 gaaagactac aaagatgacg atgacaaggg tagcggcgct accaacttca gcctgctgaa
     5761 gcaggcgggc gacgtggagg aaaatccggg cccgatggct aaaccgctga gccaggagga
     5821 aagcaccctg atcgagcgcg ctaccgccac cattaacagc atcccgatta gcgaagacta
     5881 tagcgtggcc agcgccgcgc tgagcagcga tggtcgtatt ttcaccggcg tgaacgtgta
     5941 ccactttacc ggtggcccgt gcgccgagct ggtggtgctg ggcaccgccg ccgccgccgc
     6001 cgccggcaac ctgacctgca tcgtggcgat tggtaacgaa aatcgtggca tcctgagccc
     6061 gtgcggccgt tgccgccagg tgctgctgga cctgcatccg ggcatcaagg cgattgtgaa
     6121 ggatagcgac ggccaaccga ccgccgtggg tattcgtgaa ctgctgccat ccggctatgt
     6181 ttgggagggc taaggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag
     6241 tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6301 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6361 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6421 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6481 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6541 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6601 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6661 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6721 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6781 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6841 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6901 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6961 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7021 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7081 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7141 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7201 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7261 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7321 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     7381 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7441 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7501 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7561 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7621 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7681 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7741 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7801 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7861 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7921 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     7981 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8041 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8101 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8161 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     8221 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     8281 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8341 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8401 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8461 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     8521 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9361 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10681 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
    10741 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgctt
//
