LOCUS       sequence-267836-       12573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rat Myo 9a (Myr 7) in mammalian cells.GAP minus 
            mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Chieregatti E, Gartner A, Stoffler HE, Bahler M
  TITLE     Myr 7 is a novel myosin IX-RhoGAP expressed in rat brain.
  JOURNAL   J Cell Sci. 1998 Dec 18;111 ( Pt 24):3597-608.
  PUBMED    9819351
REFERENCE   2  (bases 1 to 12573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "1998-12-18"; volume: "111 ( Pt 24)"; pages: "3597-608"
COMMENT     Sequence Label: Myo 9a-GFP R2166M
FEATURES             Location/Qualifiers
     source          1..12573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      8513..8522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8519..8521,8522..8524,8525..9238)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8519..8521,8522..8524,8525..9235)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8564..8585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8825..8844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9172..9193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    9361..9482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9398..9417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9452..9471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9489..9944)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9626..9647)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9838..9857
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9971..10075
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10077..10434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10077..10268
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10100..10120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10285..10420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10469..11263
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10469..11263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10523..10542)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11133..11152
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11451..11470)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11495..11542
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11871..12459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12360..12379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcggcgcagc
      661 cagagttctt ttgccaagaa aatggtttgc ccagtcctga acacatactg tcctttctga
      721 tgggacaaga tccaatatga atgtaagtga tggaggaaga cgacgctttg aggataatga
      781 acacacattg cgaatatatc ctgggacaat ttcagaagga acaatttact gtccaattcc
      841 tgccagaaaa aactccacag ctgctgaggt gattgactcc cttataaaca gacttcacct
      901 agacaagaca aaatgctacg ttttagcaga ggtaaaggaa tttggtggag aagaatggat
      961 tctcaatcca accgactgtc cagttcagcg aatgatgctg tggccccgaa tggctttgga
     1021 aaatcgccta agtggggagg actatcgctt tcttctgaga gaaaaaaacc tcgatggatc
     1081 aattcattat ggtagcctcc agtcatggct acgggtaacc gaagaacgcc gaaggatgat
     1141 ggagcggggt tttcttccac agcctcaaca gaaagacttt gatgatttat gtagcttgcc
     1201 tgatttgaat gagaaaactc tcttagaaaa cctacggaat cgctttaagc atgaaaaaat
     1261 ttatacttat gttggcagta ttctaatagc tattaatcca ttcaaatttc ttcctattta
     1321 taaccccaaa tatgtcaaaa tgtatgataa ccaccagctg ggaaaacttg aaccccacat
     1381 atatgctgtg gcggatgttg cttatcatgc catgcttcag cgcaaaaaga atcagtgtat
     1441 tgtgatttca ggagagagtg gttctgggaa gactcaaagc acaaactttc ttattcatca
     1501 ccttactgcc ctcagccaga aaggatttgc cagtggagta gaacagatta ttcttggagc
     1561 tggaccagta cttgaggcct ttggaaatgc aaagacagct cataataaca attcaagtcg
     1621 ttttggcaag tttattcaag taaattacca agaaacaggc actgtacttg gggcctatgt
     1681 tgaaaaatat cttctggaaa aatctagact tgtttatcaa gagcataatg aacggaacta
     1741 tcatgtattc tattacctcc ttgcaggagc aagtgaagaa gagaggttag cattccacct
     1801 taagcagcct gaggagtatc attttctcaa ccaggattgc ttcacagtgg agggagaaga
     1861 tttgaggcat gattttgaac gcttacaatt ggccatggaa atggtaggat ttcttcccaa
     1921 gacacgaaga cagattttct cactgctctc agcaatacta catttgggta atatctcata
     1981 taaaaagaag acctatcgag atgactctat tgatatctgt aatcctgaag tactgcctat
     2041 tgtctcagaa ttactagaag ttaaagaaga gatgctattt gaagcattgg ttacaaggaa
     2101 gacggtgaca gtgggtgaaa agcttatttt accatataaa ctggcagagg ctgtgacagt
     2161 gagaaactcc atggctaaat ctttatatag tgctctgttt gactggatag tgtttcgtat
     2221 taatcatgca cttctgaata gtaaagattt ggaaaaagat actaagactt tgtccattgg
     2281 tgtacttgat atttttgggt ttgaagatta tgaaaacaat agttttgaac agttctgtat
     2341 taattttgct aatgaacgtt tacagcacta ctttaatcag catatcttta agttggagca
     2401 agaggaatat agaactgaag gtatcagttg gcacaacatt gattatattg ataatacttg
     2461 ttgtataaat cttattagca aaaaaccaac aggactgctc caccttttgg atgaagaaag
     2521 caactttcca caggctacaa atcaaacatt acttgacaag tttaaacatc aacatgaaga
     2581 gaattcttac attgaatttc cagctgtaat ggagcctgct tttatcataa aacattacgc
     2641 tggaaaagta aaatatggag taaaggattt tcgggaaaaa aatacagatc atatgcgtcc
     2701 agacattgtg gctcttctga gaagtagcag gaatgctttt gtttctggga tgactggcat
     2761 tgatcccgta gctgttttcc gatgggcagt tctccgagca ttttttagag ctgtggttgc
     2821 tttcagggaa gctgggaaaa gacatattca gagaaaaagt ggacatgatg atacaacacc
     2881 atgtacaatt ttgaaaagta tggatagttt tagctttctc caacacccag tccaccagag
     2941 gagcttagag attctgcaga ggtgcaagga agagaagtac agtataaccc ggaaaaaccc
     3001 aagaacacct ctttctgatc ttcaaggcat gaacactctg aatgaaaaaa atcaacatga
     3061 tacatttgat attgcctgga atgtcagaac tggaattcgc caaagcagac taccaactaa
     3121 caatacttcc ttgcttgata aagatgggat atttgctaat tcagcaagca gcaaactcct
     3181 ggaaagagct catggaattc tcacgagaaa caaaaatttc aggtcaaaac ctgttcttcc
     3241 aaagcacttg ctggaggtaa attctttgaa acatctgacc agactgaccc ttcaggatcg
     3301 aattaccaaa tctcttcttc atttgcataa gaagaagaaa cctcccagta tcagcgccca
     3361 gtttcaggtc tccttaagca agctgatgga gacacttgat caagcagaac catattttgt
     3421 aaaatgcatt cgctcaaatg ctgaaaagct gcccttaaga ttcagtgatg ccttggtact
     3481 aagacagctt cggtacactg ggatgctgga gacagttaga attcgccaat ctggatatag
     3541 ctccaaatat tctttccagg attttgtgag ccactttcat gtacttcttc cccaacatat
     3601 tatcccatca aaatttaaca ttcaagattt cttcagaaaa ataaatatca atcctgataa
     3661 ttatcaagtt ggaaaaacca tggtgttcct aaaggagcat gaacgacagc acttacagga
     3721 tctgcttcat caggaagtgc ttcgcagaat catactgctg cagcggtggt tcagggtctt
     3781 gctctctagg cagcagttcc ttcacttgag acaggcgtct gttattattc agcgattctg
     3841 gaggaactat ctaaatcaga agcaagttag aaatgcggct gtggagaaag atgctttcat
     3901 catggctagt gcagcttccc ttcttcaagc gtcctggcgt gctcacttag agaggcagcg
     3961 gtacttggag ttacgagctg cagctgtcat tatccagcag agatggaggg aactctgtag
     4021 gcgtagacac agggctgcta cctgtataca gtcaagatgg aggggctaca ggcaaagtaa
     4081 gaagtacaaa gaacaaagga ataaaattat cctgttacag tcaatatata gaggattcag
     4141 agcacgacaa agatataagg ctttaaaaga ggaaaggctg aaagaaacaa aactagagca
     4201 tggattggcg cagatcaaga catgtggacc tctagaaatc cagggttcag acccttctga
     4261 gtgggaggat cgttcctttg ccaacagagt gaaagccata gaagaatgta aatctgtgat
     4321 agagagtaat cgaattagcc gggagagttc aatggacttc tcaaaggagt ctccagacaa
     4381 gcaacaggag agaggcagga gccaaagtgg tacagattta cagggggatg tgattgtaag
     4441 acagaggccc aagtcattgg aagatctaca tcagaaaaaa gtaggtcggg ccaaaagaga
     4501 aagtcggaga atgagagaat tagaacaagc tatatttagc ttagaattgc tcaaagtccg
     4561 ttctcttggt ggcatgtctc cttcagagga acgtagatgg tctacagaat tgatgcctga
     4621 aggccttcaa tctccacagg gtacacctga tagtgagagc tctcaaggga gcttagaact
     4681 tttaacctgt gatgagaacc aaaagagcaa accagagtcc ctcattttag atgatggaga
     4741 attgaaaatt tcatcaccca gtacatttac caatccaaaa tttgattcac aaaataatgc
     4801 cctcagtgcc tcaagtgaga caagctctac gttttctgga aaaggagcat cctctgactc
     4861 agagcatttg aagaatggaa ctgcagagga aaagcttgtc tatagttctc aacccatcac
     4921 ttgtaaatct cagctgagag actcttttgt ttcaagtagt ctgcctacat ttttttatat
     4981 tcctcatcaa gaacctctga aaacaagctc ccaactagat acaagtatcc aaagaaacaa
     5041 actaccagaa agagaaacca ctttgaagac aactcttact ctggatatta acagagaagc
     5101 caggaagtgt cagttttcag gtcaggtgac tcctttgaat ccagattctt catgtactgt
     5161 gcttaagaag ctagaaaagc tgaatattga aaaggagaaa aggcaaaagc agttacagca
     5221 gcagaatgaa aaagaaatga tggaacagat tcgtcagcag acagatattt tagagaagga
     5281 acgcaaggct ttcaagacaa ttgaacagtc acgaactgag gcctctctct tggcaccatc
     5341 cttttaccag tcaagacaaa aagtagagag gccatcttct ctgcatatcc aaaatactcc
     5401 aagtaaggga gaagctggtg tactaggttc cccatcagct cttgccacaa aagatagtcc
     5461 ctccattcat ttacccccaa aagaccgacc tgttaccctg ttctttgaaa gaaaaggaag
     5521 cccatgccaa tctagaactg tcaaggaatt aaccaagaca gaaagaatgg gtacccaaca
     5581 tgatgctgcc tgtagactct ccaataatca caatacagaa agagaacact ttaagtcaac
     5641 tcactcttac agccacagat ctgatgaccc ttccagagag ggaagttcga gacctatttt
     5701 cttcacacca aaagacaatg tgattactcc ccttgtacac agtggaaatc ctcaagtcca
     5761 caaacaagat gaaccagctt ggaaatctaa gttagcaggg cctgggcaac gagaggttgc
     5821 cagaccggcc cataaaaaga aagctcgaat ggcgcggacg cgctccgatt tcttgactag
     5881 aggtactttt gccgatgggg agggggatac agaggaagat gattacgatg acattattga
     5941 gccccttctc tcccttgacc aagcttctca ctctgagcta gggcctgtat ccagcctggg
     6001 tcaggcctct cacagtgact ccgaaatgac gtcacaacga ttttcttcag ttgatgaaca
     6061 agcaaggctt cataaggcta tgtctcaagg agagattaca aaattggcag gacgacagaa
     6121 gtcttcagat ttggacataa gaccccagag agcgaagatg agattctggg ccaaagggaa
     6181 acaaggagag aagaagacta ctagagtaaa acctgctccc cagtcggagg tctcatcact
     6241 ctttgctggc tcagatgtga cacctgttca tccattttct gatgagttaa ctcagtacca
     6301 cccaacacct cctttgagtc cagaactgcc cgggagttgt cgaaaagagt tcaaagagaa
     6361 taaggaacca tcgccaaagg caaaacgcaa gagaggtgtg aaaatcagca gtgtggcctt
     6421 ggactctatg cactggcaaa atgactctgt ccaaatcata gcaagtgcca acgatttaaa
     6481 aagtatggat gagtttcttc tgaaaaagat gaatgaccta gataatgaag acagcaagaa
     6541 ggatacatta gtggatgttg tatttaaaaa agccctcaaa gaatttcggc aaaatatctt
     6601 cagctcttac tcgtctgcac tggcgatgga tgatgggaaa agcatacgct ataaagatct
     6661 gtatgcgcta tttgaacaga tcctggaaaa gaccatgagg tttgagcaac gtgattggaa
     6721 tgaatctcca gtgagagttt ggattaacac ttttaaggtg tttttagatg aatacatgaa
     6781 tgaattcaag actttggata gcacagcccc aaaggtgctt aaaacagaaa gaaagaaaag
     6841 gaggaaaaag gaaactgatt tggtggaaga gcacaatggc cacatgttta aggccaccca
     6901 gtacagcatc cctacctact gtgaatactg ttcttccttg atatggataa tggacagagc
     6961 ctctgtttgc aaattgtgta agtacgcttg ccataagaag tgctgtctga aaaccacagc
     7021 caagtgttct aagaagtatg atccagagct gtcatctcgg caatttgggg ttgagctgtc
     7081 ccgtctgact agtgaagacc gagctgttcc tttagtggta gagaagctca taaactacat
     7141 tgaaatgcat ggactttata cagagggtat ttacatgaag tctggttcaa ctaataaaat
     7201 caaggagctt cgacaaggtc tagatacaga tgctgaaagt gtaaacctag atgactataa
     7261 catacatgtc attgcaagtg tattcaaaca atggcttcgt gatttgccca atccactcat
     7321 gacttttgaa ctctatgaag aattccttcg agctatgggc cttcaggaaa gaaaggagac
     7381 aatccgtggt gtgtactctg taattgatca gctctcccgg actcacctca gtacactgga
     7441 acgccttatc tttcatctag ttaggattgc tctacaggaa gacactaata gaatgtctgc
     7501 taatgctttg gccatcgtgt ttgcaccttg cattctccgt tgccctgaca ctactgaccc
     7561 actacaaagt gttcaagata tcagtaagac taccacatgt gtggaactga tagttgtaga
     7621 acaaatgaat aaatacaagg ctcgtctcaa agacatcagt agcctggaat ttgctgaaaa
     7681 taaagcaaag accagactat cactgattcg tagatcaatg ggaaaggggc gtctacatcg
     7741 aggaagccat ccaaatccat cctcccctgt tatagtccga ttgccttcca tgtctgatgt
     7801 cccagaagag accttgacta gtgagacagc catggacact gacgtcacag accagcagca
     7861 agcagcaatg cagcaggagg agaaagtact gactgagcag attgagaacc tacagaaaga
     7921 aaaggaagaa ctaacatttg agatgcttgt attggagcct cgtgcctctg atgatgaagc
     7981 ccttgagtct gaggcttcta ttgggactgc tgatagctcg gaaaatttga atatggatcc
     8041 tgaagagaga agcttagccc ttagctccct gaaagcagct ggcaagtctg agccttcaag
     8101 caagtttcga aagcagctaa gaaagcagcc agactctttg gactcagtca gctcctccgt
     8161 ttcttcatgt ctatcaaaca ctacatcatc tcatggcact agaaagcgat ttcagattta
     8221 ttccaagtct ccattttacc gagctgcctc agcttgtgag gcccagggaa tggaaggacc
     8281 actaggccaa gccaaatccc tagaagacag gcctcagttc atcagcagag gaaccttcaa
     8341 ccctgaaaag ggcaaacaaa aactaaagaa tgtgaaaaac tcacctcaga aaaccaaaga
     8401 gaccccagag gggacggttt catctggccg aaagaaaact gtggactcag actgcagctc
     8461 cacacagcaa ctaccactgt ttggaaataa tgagtttatg gtcccaccgg tcgccaccat
     8521 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     8581 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     8641 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     8701 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     8761 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     8821 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     8881 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     8941 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     9001 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     9061 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     9121 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     9181 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     9241 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     9301 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     9361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9481 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9541 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9601 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9661 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9721 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9781 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9841 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9901 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     9961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
    10081 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    10141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    10201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
    10441 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
    10501 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
    10561 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
    10621 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
    10681 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
    10741 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
    10801 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
    10861 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
    10921 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
    10981 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
    11041 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
    11101 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
    11161 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
    11221 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
    11281 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
    11341 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    11401 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
    11461 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
    11521 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
    11581 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
    11641 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
    11701 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
    11761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12001 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    12061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12541 gattctgtgg ataaccgtat taccgccatg cat
//
